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The map label for this gene is amiC [H]

Identifier: 157372046

GI number: 157372046

Start: 4211180

End: 4212433

Strand: Reverse

Name: amiC [H]

Synonym: Spro_3811

Alternate gene names: 157372046

Gene position: 4212433-4211180 (Counterclockwise)

Preceding gene: 157372048

Following gene: 157372045

Centisome position: 77.31

GC content: 55.26

Gene sequence:

>1254_bases
ATGCCAAACTCAAATCACAATCTGGGCCGCCGTCGTTTATTACAGGGGGTTGCCGCAAGCTGGTTGCTGAGTGTGAGTCG
CACAGGTTTTGCTGCTTCATCGCACGTCATTGCCGTACGCGTCTGGCCATCCTCCACCTATACCCGGGTGACGCTCGAAT
CCAACGTTGAACTGAAATATAAGCAGTTTGCGCTGACCAATCCCGATCGCGTGGTGGTGGATATTGAAGGCGTGCAGCTC
AACAGCGTGCTGAAGGGGATTGTTGGCCAGGTTCGTGCCGACGATCCCTACCTGAAACAGGCGCGCGTTGGTCAGTTTGA
TCAAAACACCGTCCGGTTGGTGCTGGAGCTTAAGCGCAGCGTCAGCCCGCACATGTTTACTCTGGCGCCGGTGCCGGAAT
ACCGCAATCGTCTGGTGATGGATCTGTACCCGAGCAAGGGCGGCAGCGGTGAAGACTATGATCCGCTGTTGGCGTTACTG
GAAGATTACAACAAGGGCGATCTTGAGCGCACATTGCCGGCGGAAGCGCCACAGGCCGGTAAGGCGGGGCGTGACCGACC
GATTGTCATCATGCTGGATCCCGGTCACGGCGGCGAAGATCCCGGAGCGATTGGTAAATTCAAAACGCGTGAAAAAGACA
TCGTATTGCAGATTGCACGCCGGCTAAGCACGTTAATCAAGCGCGAGCCGAATATGAAGGTGTTTATGACGCGCAATGAA
GACGTCTTCATTCCGTTGAAGGTGAGGGTGGCGAAGGCGCGTAAGCAGCGTGCCGACCTGTTTGTGTCCATCCACGCCGA
TGCCTTTACCAGCCGGGCCGCGCGTGGGTCATCGGTGTTTGCGCTGTCGACCAAAGGTGCCACCAGTTCGGCGGCGAAGT
TCCTGGCGCAGACCCAAAATGAATCGGACCAAATCGGCGGCGTAAGCAAAAGCGGTGACCGCTATCTGGATCACACCATG
TTTGATCTGATTCAAACCGCGACCATCAATGACAGCCTGAAATTCGGTAAGGAAGTGCTGAACCGGATGGGAAAAATAAA
CCGTTTGCATAAGAATCGTGTCGATCAGGCCGGTTTTGCGGTATTGAAAGCGCCGGACATCCCTTCGATTCTGGTTGAGA
CGGCGTTTATCAGCAACATTGAAGAAGAACGCAAGCTGCGTACCACTCATTTCCAGCAGCAGGTGGCGGAATCTATTCTG
GCGGGTATCAAGGCGTACTTTGCCAACGGTGGTGCGGTAGCGCGCAGAGGGTAA

Upstream 100 bases:

>100_bases
TTCTGATGACAGCGCCGGAATGATTGGTTAAAGTTTTCGGCATTCCTGTTAACTGGTGTTTTTTTCATCTGTTTTAATCT
GTTACCACTGCTGGAGCCGC

Downstream 100 bases:

>100_bases
GGCTAATGCGGAAACGACTGGGCGGCTGCCCGGTCGATTTTTGGCGGATTTCAGATACAAAAAAACACCCGTTAAGGTGC
TTATTTGTAGTCATTAAGAT

Product: N-acetylmuramoyl-L-alanine amidase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 417; Mature: 416

Protein sequence:

>417_residues
MPNSNHNLGRRRLLQGVAASWLLSVSRTGFAASSHVIAVRVWPSSTYTRVTLESNVELKYKQFALTNPDRVVVDIEGVQL
NSVLKGIVGQVRADDPYLKQARVGQFDQNTVRLVLELKRSVSPHMFTLAPVPEYRNRLVMDLYPSKGGSGEDYDPLLALL
EDYNKGDLERTLPAEAPQAGKAGRDRPIVIMLDPGHGGEDPGAIGKFKTREKDIVLQIARRLSTLIKREPNMKVFMTRNE
DVFIPLKVRVAKARKQRADLFVSIHADAFTSRAARGSSVFALSTKGATSSAAKFLAQTQNESDQIGGVSKSGDRYLDHTM
FDLIQTATINDSLKFGKEVLNRMGKINRLHKNRVDQAGFAVLKAPDIPSILVETAFISNIEEERKLRTTHFQQQVAESIL
AGIKAYFANGGAVARRG

Sequences:

>Translated_417_residues
MPNSNHNLGRRRLLQGVAASWLLSVSRTGFAASSHVIAVRVWPSSTYTRVTLESNVELKYKQFALTNPDRVVVDIEGVQL
NSVLKGIVGQVRADDPYLKQARVGQFDQNTVRLVLELKRSVSPHMFTLAPVPEYRNRLVMDLYPSKGGSGEDYDPLLALL
EDYNKGDLERTLPAEAPQAGKAGRDRPIVIMLDPGHGGEDPGAIGKFKTREKDIVLQIARRLSTLIKREPNMKVFMTRNE
DVFIPLKVRVAKARKQRADLFVSIHADAFTSRAARGSSVFALSTKGATSSAAKFLAQTQNESDQIGGVSKSGDRYLDHTM
FDLIQTATINDSLKFGKEVLNRMGKINRLHKNRVDQAGFAVLKAPDIPSILVETAFISNIEEERKLRTTHFQQQVAESIL
AGIKAYFANGGAVARRG
>Mature_416_residues
PNSNHNLGRRRLLQGVAASWLLSVSRTGFAASSHVIAVRVWPSSTYTRVTLESNVELKYKQFALTNPDRVVVDIEGVQLN
SVLKGIVGQVRADDPYLKQARVGQFDQNTVRLVLELKRSVSPHMFTLAPVPEYRNRLVMDLYPSKGGSGEDYDPLLALLE
DYNKGDLERTLPAEAPQAGKAGRDRPIVIMLDPGHGGEDPGAIGKFKTREKDIVLQIARRLSTLIKREPNMKVFMTRNED
VFIPLKVRVAKARKQRADLFVSIHADAFTSRAARGSSVFALSTKGATSSAAKFLAQTQNESDQIGGVSKSGDRYLDHTMF
DLIQTATINDSLKFGKEVLNRMGKINRLHKNRVDQAGFAVLKAPDIPSILVETAFISNIEEERKLRTTHFQQQVAESILA
GIKAYFANGGAVARRG

Specific function: Cell-wall hydrolase involved in septum cleavage during cell division [H]

COG id: COG0860

COG function: function code M; N-acetylmuramoyl-L-alanine amidase

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 3 family [H]

Homologues:

Organism=Escherichia coli, GI87082163, Length=419, Percent_Identity=74.7016706443914, Blast_Score=652, Evalue=0.0,
Organism=Escherichia coli, GI1788776, Length=232, Percent_Identity=46.551724137931, Blast_Score=213, Evalue=2e-56,
Organism=Escherichia coli, GI1790611, Length=225, Percent_Identity=42.6666666666667, Blast_Score=185, Evalue=6e-48,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR021731
- InterPro:   IPR002508
- InterPro:   IPR006311 [H]

Pfam domain/function: PF01520 Amidase_3; PF11741 AMIN [H]

EC number: =3.5.1.28 [H]

Molecular weight: Translated: 46108; Mature: 45977

Theoretical pI: Translated: 10.48; Mature: 10.48

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPNSNHNLGRRRLLQGVAASWLLSVSRTGFAASSHVIAVRVWPSSTYTRVTLESNVELKY
CCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCEEEEEEECCCEEEE
KQFALTNPDRVVVDIEGVQLNSVLKGIVGQVRADDPYLKQARVGQFDQNTVRLVLELKRS
EEEEECCCCEEEEEECCCCHHHHHHHHHHCCCCCCHHHHHHHCCCCCCHHHHHHHHHHHC
VSPHMFTLAPVPEYRNRLVMDLYPSKGGSGEDYDPLLALLEDYNKGDLERTLPAEAPQAG
CCCCEEEEECCHHHHCCEEEEEECCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCC
KAGRDRPIVIMLDPGHGGEDPGAIGKFKTREKDIVLQIARRLSTLIKREPNMKVFMTRNE
CCCCCCCEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEECCC
DVFIPLKVRVAKARKQRADLFVSIHADAFTSRAARGSSVFALSTKGATSSAAKFLAQTQN
CEEEEEEEHHHHHHHHCCCEEEEEECHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHCCC
ESDQIGGVSKSGDRYLDHTMFDLIQTATINDSLKFGKEVLNRMGKINRLHKNRVDQAGFA
CCHHCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHCEE
VLKAPDIPSILVETAFISNIEEERKLRTTHFQQQVAESILAGIKAYFANGGAVARRG
EEECCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
PNSNHNLGRRRLLQGVAASWLLSVSRTGFAASSHVIAVRVWPSSTYTRVTLESNVELKY
CCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCEEEEEEECCCEEEE
KQFALTNPDRVVVDIEGVQLNSVLKGIVGQVRADDPYLKQARVGQFDQNTVRLVLELKRS
EEEEECCCCEEEEEECCCCHHHHHHHHHHCCCCCCHHHHHHHCCCCCCHHHHHHHHHHHC
VSPHMFTLAPVPEYRNRLVMDLYPSKGGSGEDYDPLLALLEDYNKGDLERTLPAEAPQAG
CCCCEEEEECCHHHHCCEEEEEECCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCC
KAGRDRPIVIMLDPGHGGEDPGAIGKFKTREKDIVLQIARRLSTLIKREPNMKVFMTRNE
CCCCCCCEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEECCC
DVFIPLKVRVAKARKQRADLFVSIHADAFTSRAARGSSVFALSTKGATSSAAKFLAQTQN
CEEEEEEEHHHHHHHHCCCEEEEEECHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHCCC
ESDQIGGVSKSGDRYLDHTMFDLIQTATINDSLKFGKEVLNRMGKINRLHKNRVDQAGFA
CCHHCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHCEE
VLKAPDIPSILVETAFISNIEEERKLRTTHFQQQVAESILAGIKAYFANGGAVARRG
EEECCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]