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The map label for this gene is kgtP [H]

Identifier: 157371986

GI number: 157371986

Start: 4142187

End: 4143497

Strand: Reverse

Name: kgtP [H]

Synonym: Spro_3751

Alternate gene names: 157371986

Gene position: 4143497-4142187 (Counterclockwise)

Preceding gene: 157371987

Following gene: 157371980

Centisome position: 76.04

GC content: 52.33

Gene sequence:

>1311_bases
ATGACAGAAACAACCTCGGCCATTGAGGATGAAGGGACCGCCGATCTGGCTGTTCAGGACAAAAAGAAACGTATTTTCGC
GATTGTTGGGGCTTCATCAGGGAATCTGGTGGAGTGGTTTGATTTTTACGTCTATTCCTTCTGCTCTATCTACTTTGCCG
CCTCGTTTTTCCCCGCCGGTAACAGCACTACCCAGCTGCTGCAAACGGCCGGTGTGTTTGCCGCCGGTTTCTTTATGCGC
CCAATTGGTGGCTGGTTGTTCGGTTATATTGCCGACAAGCATGGCCGTAAAAACTCGATGCTGATCTCGGTCTGTATGAT
GTGCGCCGGATCGCTGGTGATCGCTTGCTTACCGACCTACGAGTCGATCGGCAGTTGGGCACCGGTGCTGCTGCTGGTTG
CACGCCTGTTCCAGGGGCTGTCGGTCGGCGGTGAATATGGTACCAGTGCCACCTATATGAGTGAGGTGGCCATAAAGGGG
CGACGCGGGTTCTATGCCTCTTTCCAATATGTCACGCTGATTGGCGGCCAACTGTTGGCGTTGCTGGTTTTGGTGGCGCT
ACAACAAATCCTGTCCACGGAAGAGCTCAAAGCCTGGGGCTGGCGTATTCCCTTCGCGATAGGAGCGGTATTGGCCGTAG
TGGCGTTGTATCTGCGCCGTTCACTGAATGAAACTTCCGATGCCGAAACCCGTAACCATAAGGATGCCGGTTCGCTGAAA
GGGCTGTGGAAGAATCGCAAAGCCTTCCTGATGGTGCTGGGGTTTACCGCCGGTGGCTCGCTGAGTTTTTATACTTTTAC
CACCTACATGCAAAAGTATCTGGTCAATACCTCAGGGATGGATGCCAAGCTGGCCAGCGGTATTATGACTGCAGCCTTGT
TTATCTATATGCTGCTGCAACCGTTGGTTGGGGCACTATCGGACAAGATTGGTCGCCGTAGCTCAATGCTGATCTTTGCC
TCTTTCGCCACCTTACTCACAGTACCTATTCTGTTAACCTTGAAAAATGTCAGCAATCCCTATGCGGCATTCGGTCTGAT
CGTACTGGCACTGTTTATTGTCAGTTTTTATACTTCGATCAGTGGGTTGCTGAAAGCCGAAATGTTCCCGCCGGAAGTGC
GGGCGCTAGGCGTTGGTCTGTCTTATGCGGTGGCTAACGCGATGTTTGGCGGGTCTGCAGAATATGTTGCCCTGTCGCTG
AAGTCATATGGCATAGAGAATGCCTTCTTCTGGTACGTTTCCGGCATGTGTCTGTTGGCGCTGATCGTTTCACTTCGCTT
GCATCGCAAGGGGAAAGAGATCCAACTGTGA

Upstream 100 bases:

>100_bases
CCCGCTATTTGTTTTAATCGTTAACTAAATACTAACAATTGAATCACATTTAAACGGCAGGACGCCGACTTCACTGTTTA
TCTGGCTCGGAGAATATAAA

Downstream 100 bases:

>100_bases
ATCAACGGGTGGCGTGGTACAGGCTATACCCCGTAGCCGCCCCCGCCACATCCCAGGCAAAATCCTTCCAGCTCCAGCCG
GTGCCGCCTTCGCGACTGTC

Product: alpha-ketoglutarate transporter

Products: Proton [Cytoplasm]; 2-oxoglutarate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 436; Mature: 435

Protein sequence:

>436_residues
MTETTSAIEDEGTADLAVQDKKKRIFAIVGASSGNLVEWFDFYVYSFCSIYFAASFFPAGNSTTQLLQTAGVFAAGFFMR
PIGGWLFGYIADKHGRKNSMLISVCMMCAGSLVIACLPTYESIGSWAPVLLLVARLFQGLSVGGEYGTSATYMSEVAIKG
RRGFYASFQYVTLIGGQLLALLVLVALQQILSTEELKAWGWRIPFAIGAVLAVVALYLRRSLNETSDAETRNHKDAGSLK
GLWKNRKAFLMVLGFTAGGSLSFYTFTTYMQKYLVNTSGMDAKLASGIMTAALFIYMLLQPLVGALSDKIGRRSSMLIFA
SFATLLTVPILLTLKNVSNPYAAFGLIVLALFIVSFYTSISGLLKAEMFPPEVRALGVGLSYAVANAMFGGSAEYVALSL
KSYGIENAFFWYVSGMCLLALIVSLRLHRKGKEIQL

Sequences:

>Translated_436_residues
MTETTSAIEDEGTADLAVQDKKKRIFAIVGASSGNLVEWFDFYVYSFCSIYFAASFFPAGNSTTQLLQTAGVFAAGFFMR
PIGGWLFGYIADKHGRKNSMLISVCMMCAGSLVIACLPTYESIGSWAPVLLLVARLFQGLSVGGEYGTSATYMSEVAIKG
RRGFYASFQYVTLIGGQLLALLVLVALQQILSTEELKAWGWRIPFAIGAVLAVVALYLRRSLNETSDAETRNHKDAGSLK
GLWKNRKAFLMVLGFTAGGSLSFYTFTTYMQKYLVNTSGMDAKLASGIMTAALFIYMLLQPLVGALSDKIGRRSSMLIFA
SFATLLTVPILLTLKNVSNPYAAFGLIVLALFIVSFYTSISGLLKAEMFPPEVRALGVGLSYAVANAMFGGSAEYVALSL
KSYGIENAFFWYVSGMCLLALIVSLRLHRKGKEIQL
>Mature_435_residues
TETTSAIEDEGTADLAVQDKKKRIFAIVGASSGNLVEWFDFYVYSFCSIYFAASFFPAGNSTTQLLQTAGVFAAGFFMRP
IGGWLFGYIADKHGRKNSMLISVCMMCAGSLVIACLPTYESIGSWAPVLLLVARLFQGLSVGGEYGTSATYMSEVAIKGR
RGFYASFQYVTLIGGQLLALLVLVALQQILSTEELKAWGWRIPFAIGAVLAVVALYLRRSLNETSDAETRNHKDAGSLKG
LWKNRKAFLMVLGFTAGGSLSFYTFTTYMQKYLVNTSGMDAKLASGIMTAALFIYMLLQPLVGALSDKIGRRSSMLIFAS
FATLLTVPILLTLKNVSNPYAAFGLIVLALFIVSFYTSISGLLKAEMFPPEVRALGVGLSYAVANAMFGGSAEYVALSLK
SYGIENAFFWYVSGMCLLALIVSLRLHRKGKEIQL

Specific function: Uptake of alpha-ketoglutarate across the boundary membrane with the concomitant import of a cation (symport system) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1788942, Length=430, Percent_Identity=77.6744186046512, Blast_Score=653, Evalue=0.0,
Organism=Escherichia coli, GI1790550, Length=373, Percent_Identity=31.9034852546917, Blast_Score=206, Evalue=2e-54,
Organism=Escherichia coli, GI1788292, Length=422, Percent_Identity=30.3317535545024, Blast_Score=189, Evalue=3e-49,
Organism=Escherichia coli, GI1789941, Length=449, Percent_Identity=31.6258351893096, Blast_Score=167, Evalue=2e-42,
Organism=Escherichia coli, GI87082231, Length=208, Percent_Identity=30.2884615384615, Blast_Score=82, Evalue=6e-17,
Organism=Escherichia coli, GI87082404, Length=345, Percent_Identity=24.0579710144928, Blast_Score=77, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 47400; Mature: 47269

Theoretical pI: Translated: 9.46; Mature: 9.46

Prosite motif: PS50850 MFS ; PS00216 SUGAR_TRANSPORT_1 ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTETTSAIEDEGTADLAVQDKKKRIFAIVGASSGNLVEWFDFYVYSFCSIYFAASFFPAG
CCCCCCCCCCCCCCCEEEECCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCC
NSTTQLLQTAGVFAAGFFMRPIGGWLFGYIADKHGRKNSMLISVCMMCAGSLVIACLPTY
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCH
ESIGSWAPVLLLVARLFQGLSVGGEYGTSATYMSEVAIKGRRGFYASFQYVTLIGGQLLA
HHHCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHH
LLVLVALQQILSTEELKAWGWRIPFAIGAVLAVVALYLRRSLNETSDAETRNHKDAGSLK
HHHHHHHHHHHCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHCCCCCCHHHH
GLWKNRKAFLMVLGFTAGGSLSFYTFTTYMQKYLVNTSGMDAKLASGIMTAALFIYMLLQ
HHHCCCCEEEEEEEECCCCCEEHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHH
PLVGALSDKIGRRSSMLIFASFATLLTVPILLTLKNVSNPYAAFGLIVLALFIVSFYTSI
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
SGLLKAEMFPPEVRALGVGLSYAVANAMFGGSAEYVALSLKSYGIENAFFWYVSGMCLLA
HHHHHHHCCCCHHHHHHCCHHHHHHHHHCCCCCHHEEEEHHHCCCCHHHHHHHHHHHHHH
LIVSLRLHRKGKEIQL
HHHHHHHHCCCCCCCC
>Mature Secondary Structure 
TETTSAIEDEGTADLAVQDKKKRIFAIVGASSGNLVEWFDFYVYSFCSIYFAASFFPAG
CCCCCCCCCCCCCCEEEECCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCC
NSTTQLLQTAGVFAAGFFMRPIGGWLFGYIADKHGRKNSMLISVCMMCAGSLVIACLPTY
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCH
ESIGSWAPVLLLVARLFQGLSVGGEYGTSATYMSEVAIKGRRGFYASFQYVTLIGGQLLA
HHHCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHH
LLVLVALQQILSTEELKAWGWRIPFAIGAVLAVVALYLRRSLNETSDAETRNHKDAGSLK
HHHHHHHHHHHCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHCCCCCCHHHH
GLWKNRKAFLMVLGFTAGGSLSFYTFTTYMQKYLVNTSGMDAKLASGIMTAALFIYMLLQ
HHHCCCCEEEEEEEECCCCCEEHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHH
PLVGALSDKIGRRSSMLIFASFATLLTVPILLTLKNVSNPYAAFGLIVLALFIVSFYTSI
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
SGLLKAEMFPPEVRALGVGLSYAVANAMFGGSAEYVALSLKSYGIENAFFWYVSGMCLLA
HHHHHHHCCCCHHHHHHCCHHHHHHHHHCCCCCHHEEEEHHHCCCCHHHHHHHHHHHHHH
LIVSLRLHRKGKEIQL
HHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; 2-oxoglutarate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + 2-oxoglutarate [Periplasm] = Proton [Cytoplasm] + 2-oxoglutarate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2118499; 9205837; 9278503; 1709493; 2190187; 2053984; 8419306 [H]