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The map label for this gene is yfiF [H]

Identifier: 157371980

GI number: 157371980

Start: 4135012

End: 4136112

Strand: Reverse

Name: yfiF [H]

Synonym: Spro_3745

Alternate gene names: 157371980

Gene position: 4136112-4135012 (Counterclockwise)

Preceding gene: 157371986

Following gene: 157371972

Centisome position: 75.91

GC content: 59.4

Gene sequence:

>1101_bases
ATGAACGATTCATTTAGTGGCAAGAACGGTAAAGTTAAAGTGATGTACGTCCGCAGTGACGACGACAACAACAGCGACGA
CCGTAACAAGAATAAGCGTCCGGCCGGCAAAGGCCGTCCGGCAGACGGTGCGCGCTCTTCGGGCCGTACCGGTCAGGACA
AGCCGCGCGGTAATAACGATCGCCGTGGCTCTGGCTCTGATTCCCGCCGCAGTGAAAGCGATCGTCCTCGCCGTCCAGCG
CACAATGAAGACCGTGGCGGTTATGATTCGCCGTGGAAAACCGTGTCACGTGGCCCGGAAGAAGAGCCGGCGTTTGATCA
CGGCGGCATCAGCGGCAAGAGCTTTATCGATCCAGAACAGTTACGCCGTCAGCGTGCGGAAGAAACCCGCGTTTACGGTG
AAAACGCCTGTCAGGCCCTGTTTGCCAGCCGTCCGGACGCTATCGTCCGCGCCTGGTTCGTACAGTCGGTCACGCCACGT
TTTCGTGAAGCACTGCGCTGGATGGCGGCAAACCGCAAAGCCTATCACGTGGTGGATGAAGAAGAGCTGGCCAAAGCGTC
CGGCACCGAACACCACGGCGGCGTTTGCTTCCTGATTAAAAAGCGTCAGGGTCTGGATGCGCAAACCTATCTGAAAGAAG
CCCCGGCGAAAGACTGCGTGCTGGCGCTGGAAGAAGTAGGCAACCCGCACAACCTGGGTGCTATCGTGCGTTCTTGCGCT
CACTTTGGCGTGAACGGCGTGTTGCTGCAGGATCCGGCGGTGCTGGAATCAGGTGCTGCGGTGCGTACGGCCGAAGGCGG
CGCGGAACACATCAAGGCAATTAACGCCGATGACTTCCTGTCGGTGCTGGATACCTTCCGCAAGGCGGGTTACGCCATCG
TCACCACCTCGAGCCACAAAGGGACTCCGTTGGCGCAGGCTAAGCTGCCGGCCAAAATGGTACTGGTACTCGGCCAGGAA
CGTGACGGCCTGAGCGACAGTGCCTGGCAGCAGGGCGACATGAACGTGTCTATCGGCGGTACCGGCAAGGTGGAAAGCCT
GAACGTATCGGTCGCGACCGGCATTTTGCTGGCTGATTGGTGGCGTCAAAACCAGGCCTAA

Upstream 100 bases:

>100_bases
GTTCGATGAATACGATAGTTGTTAGCTTTTAGCGCAAGTAGGTGGCTTAGCACCCCAACATCAGGTAATCTTCGCGCCCT
GCGCGTTGGTGGAGAAGAAA

Downstream 100 bases:

>100_bases
TATACCCGTCGCCTTTCAAGCCGCAGCGTTGTTACCTGCACTAACTCACCCCAGTTACTTACTAGAGTAAGCGCCTGGGG
ATGAGTGAGTTGGTCGCCTA

Product: putative methyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 366; Mature: 366

Protein sequence:

>366_residues
MNDSFSGKNGKVKVMYVRSDDDNNSDDRNKNKRPAGKGRPADGARSSGRTGQDKPRGNNDRRGSGSDSRRSESDRPRRPA
HNEDRGGYDSPWKTVSRGPEEEPAFDHGGISGKSFIDPEQLRRQRAEETRVYGENACQALFASRPDAIVRAWFVQSVTPR
FREALRWMAANRKAYHVVDEEELAKASGTEHHGGVCFLIKKRQGLDAQTYLKEAPAKDCVLALEEVGNPHNLGAIVRSCA
HFGVNGVLLQDPAVLESGAAVRTAEGGAEHIKAINADDFLSVLDTFRKAGYAIVTTSSHKGTPLAQAKLPAKMVLVLGQE
RDGLSDSAWQQGDMNVSIGGTGKVESLNVSVATGILLADWWRQNQA

Sequences:

>Translated_366_residues
MNDSFSGKNGKVKVMYVRSDDDNNSDDRNKNKRPAGKGRPADGARSSGRTGQDKPRGNNDRRGSGSDSRRSESDRPRRPA
HNEDRGGYDSPWKTVSRGPEEEPAFDHGGISGKSFIDPEQLRRQRAEETRVYGENACQALFASRPDAIVRAWFVQSVTPR
FREALRWMAANRKAYHVVDEEELAKASGTEHHGGVCFLIKKRQGLDAQTYLKEAPAKDCVLALEEVGNPHNLGAIVRSCA
HFGVNGVLLQDPAVLESGAAVRTAEGGAEHIKAINADDFLSVLDTFRKAGYAIVTTSSHKGTPLAQAKLPAKMVLVLGQE
RDGLSDSAWQQGDMNVSIGGTGKVESLNVSVATGILLADWWRQNQA
>Mature_366_residues
MNDSFSGKNGKVKVMYVRSDDDNNSDDRNKNKRPAGKGRPADGARSSGRTGQDKPRGNNDRRGSGSDSRRSESDRPRRPA
HNEDRGGYDSPWKTVSRGPEEEPAFDHGGISGKSFIDPEQLRRQRAEETRVYGENACQALFASRPDAIVRAWFVQSVTPR
FREALRWMAANRKAYHVVDEEELAKASGTEHHGGVCFLIKKRQGLDAQTYLKEAPAKDCVLALEEVGNPHNLGAIVRSCA
HFGVNGVLLQDPAVLESGAAVRTAEGGAEHIKAINADDFLSVLDTFRKAGYAIVTTSSHKGTPLAQAKLPAKMVLVLGQE
RDGLSDSAWQQGDMNVSIGGTGKVESLNVSVATGILLADWWRQNQA

Specific function: Unknown

COG id: COG0566

COG function: function code J; rRNA methylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family [H]

Homologues:

Organism=Escherichia coli, GI1788935, Length=368, Percent_Identity=64.945652173913, Blast_Score=460, Evalue=1e-131,
Organism=Escherichia coli, GI1790623, Length=237, Percent_Identity=27.8481012658228, Blast_Score=78, Evalue=8e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001537
- InterPro:   IPR013123
- InterPro:   IPR016479 [H]

Pfam domain/function: PF00588 SpoU_methylase; PF08032 SpoU_sub_bind [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 39876; Mature: 39876

Theoretical pI: Translated: 8.87; Mature: 8.87

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNDSFSGKNGKVKVMYVRSDDDNNSDDRNKNKRPAGKGRPADGARSSGRTGQDKPRGNND
CCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
RRGSGSDSRRSESDRPRRPAHNEDRGGYDSPWKTVSRGPEEEPAFDHGGISGKSFIDPEQ
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCCCCCCCCCCCCCCCCCCHHH
LRRQRAEETRVYGENACQALFASRPDAIVRAWFVQSVTPRFREALRWMAANRKAYHVVDE
HHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCHHHHHHHHHHHHCCCEEEECCH
EELAKASGTEHHGGVCFLIKKRQGLDAQTYLKEAPAKDCVLALEEVGNPHNLGAIVRSCA
HHHHHHCCCCCCCCEEEEEECCCCCCHHHHHHHCCHHHHHHHHHHCCCCHHHHHHHHHHH
HFGVNGVLLQDPAVLESGAAVRTAEGGAEHIKAINADDFLSVLDTFRKAGYAIVTTSSHK
HHCCCCEEECCCHHHHCCCEEEECCCCHHHHEECCHHHHHHHHHHHHHCCEEEEEECCCC
GTPLAQAKLPAKMVLVLGQERDGLSDSAWQQGDMNVSIGGTGKVESLNVSVATGILLADW
CCCCHHHHCCEEEEEEECCCCCCCCCHHHCCCCCEEEECCCCCEEEEEEHHHHHHHHHHH
WRQNQA
HHCCCC
>Mature Secondary Structure
MNDSFSGKNGKVKVMYVRSDDDNNSDDRNKNKRPAGKGRPADGARSSGRTGQDKPRGNND
CCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
RRGSGSDSRRSESDRPRRPAHNEDRGGYDSPWKTVSRGPEEEPAFDHGGISGKSFIDPEQ
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCCCCCCCCCCCCCCCCCCHHH
LRRQRAEETRVYGENACQALFASRPDAIVRAWFVQSVTPRFREALRWMAANRKAYHVVDE
HHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCHHHHHHHHHHHHCCCEEEECCH
EELAKASGTEHHGGVCFLIKKRQGLDAQTYLKEAPAKDCVLALEEVGNPHNLGAIVRSCA
HHHHHHCCCCCCCCEEEEEECCCCCCHHHHHHHCCHHHHHHHHHHCCCCHHHHHHHHHHH
HFGVNGVLLQDPAVLESGAAVRTAEGGAEHIKAINADDFLSVLDTFRKAGYAIVTTSSHK
HHCCCCEEECCCHHHHCCCEEEECCCCHHHHEECCHHHHHHHHHHHHHCCEEEEEECCCC
GTPLAQAKLPAKMVLVLGQERDGLSDSAWQQGDMNVSIGGTGKVESLNVSVATGILLADW
CCCCHHHHCCEEEEEEECCCCCCCCCHHHCCCCCEEEECCCCCEEEEEEHHHHHHHHHHH
WRQNQA
HHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]