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The map label for this gene is hda [H]

Identifier: 157371756

GI number: 157371756

Start: 3893863

End: 3894474

Strand: Reverse

Name: hda [H]

Synonym: Spro_3519

Alternate gene names: 157371756

Gene position: 3894474-3893863 (Counterclockwise)

Preceding gene: 157371759

Following gene: 157371753

Centisome position: 71.47

GC content: 55.23

Gene sequence:

>612_bases
TTGAGCGCGATCCAGTCTGCCGTTCGTCAGGAACATGGCAGCTATATCTATTTTTGGTCACGCGAGGGTGGTGGGCGCAG
CCACCTGTTGCATGCGTCCTGCGCGGAGCTTTCGCTGCGTGGCGAGGCCGTAGGCTATGTGCCACTGGATAAGCGAGCCT
ATTTCGTGCCTGAAGTATTGGATGGCATGGAGCAGCTGGCCCTGGTGTGTATCGATAATATCGAATGCATTGCCGGTGAC
GAAGAGTGGGAAATGGCCATCTTCAATCTCTACAACCGCATTCTGGAAACCGGCCGTACGCGCCTGTTTATTACCGGCGA
TCGCCCGCCACGCCAATTGAATCTCCATTTGCCCGATCTGGCTTCACGCCTGGACTGGGGGCAAATCTACAAGTTACAGC
CGCTTTCGGATGAAGAGAAATTGCTGGCGTTGCAACTACGCGGCAAGCTGCGCGGTTTTGAATTACCGGAGGACGTGGGG
CGTTTCCTGCTCAAACGCCTGGATCGCGAAATGCGCACGTTGTTTATGACGCTGGATCAACTCGATCACGCTTCAATCAC
CGCCCAGCGCAAGCTGACCATCCCGTTTGTGAAAGAGATCCTGGGGCTATAA

Upstream 100 bases:

>100_bases
AGGTGCTTTTCTGAATACGCCGGCACAGCTTTCACTGCCACTTTATCTTCCCGATGATGAAACTTTTGCCAGTTTTTATC
CGGGGGAGAACCCATCCCTA

Downstream 100 bases:

>100_bases
CAGCTTCACTTCGAAAATTTTGGGCACCATAATCAGTGCCCACTATCACGTTTACAGAATTTCCAGCACCTGCTCCGGCG
GCCGGCCGATACGCGCCTTG

Product: DNA replication initiation factor

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 203; Mature: 202

Protein sequence:

>203_residues
MSAIQSAVRQEHGSYIYFWSREGGGRSHLLHASCAELSLRGEAVGYVPLDKRAYFVPEVLDGMEQLALVCIDNIECIAGD
EEWEMAIFNLYNRILETGRTRLFITGDRPPRQLNLHLPDLASRLDWGQIYKLQPLSDEEKLLALQLRGKLRGFELPEDVG
RFLLKRLDREMRTLFMTLDQLDHASITAQRKLTIPFVKEILGL

Sequences:

>Translated_203_residues
MSAIQSAVRQEHGSYIYFWSREGGGRSHLLHASCAELSLRGEAVGYVPLDKRAYFVPEVLDGMEQLALVCIDNIECIAGD
EEWEMAIFNLYNRILETGRTRLFITGDRPPRQLNLHLPDLASRLDWGQIYKLQPLSDEEKLLALQLRGKLRGFELPEDVG
RFLLKRLDREMRTLFMTLDQLDHASITAQRKLTIPFVKEILGL
>Mature_202_residues
SAIQSAVRQEHGSYIYFWSREGGGRSHLLHASCAELSLRGEAVGYVPLDKRAYFVPEVLDGMEQLALVCIDNIECIAGDE
EWEMAIFNLYNRILETGRTRLFITGDRPPRQLNLHLPDLASRLDWGQIYKLQPLSDEEKLLALQLRGKLRGFELPEDVGR
FLLKRLDREMRTLFMTLDQLDHASITAQRKLTIPFVKEILGL

Specific function: Mediates the interactions of dnaA with the beta subunit sliding clamp, promoting hydrolysis of dnaA-bound ATP. It is essential for the control of initiation of DNA replication by inhibiting reinitiation of replication [H]

COG id: COG0593

COG function: function code L; ATPase involved in DNA replication initiation

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dnaA family. HdA subfamily [H]

Homologues:

Organism=Escherichia coli, GI226510964, Length=203, Percent_Identity=85.2216748768473, Blast_Score=360, Evalue=1e-101,
Organism=Escherichia coli, GI2367267, Length=203, Percent_Identity=26.6009852216749, Blast_Score=72, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020591
- InterPro:   IPR017788
- InterPro:   IPR013317
- InterPro:   IPR022864 [H]

Pfam domain/function: PF00308 Bac_DnaA [H]

EC number: NA

Molecular weight: Translated: 23299; Mature: 23168

Theoretical pI: Translated: 6.03; Mature: 6.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAIQSAVRQEHGSYIYFWSREGGGRSHLLHASCAELSLRGEAVGYVPLDKRAYFVPEVL
CHHHHHHHHHHCCCEEEEECCCCCCCCCEEHHHHHHHEECCCEEEEEECCCCCEECHHHH
DGMEQLALVCIDNIECIAGDEEWEMAIFNLYNRILETGRTRLFITGDRPPRQLNLHLPDL
HHHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCEEEEECHHH
ASRLDWGQIYKLQPLSDEEKLLALQLRGKLRGFELPEDVGRFLLKRLDREMRTLFMTLDQ
HHHCCCCCEEEECCCCCHHHEEHEEHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
LDHASITAQRKLTIPFVKEILGL
CCCCCCCCCCEEEHHHHHHHHCC
>Mature Secondary Structure 
SAIQSAVRQEHGSYIYFWSREGGGRSHLLHASCAELSLRGEAVGYVPLDKRAYFVPEVL
HHHHHHHHHHCCCEEEEECCCCCCCCCEEHHHHHHHEECCCEEEEEECCCCCEECHHHH
DGMEQLALVCIDNIECIAGDEEWEMAIFNLYNRILETGRTRLFITGDRPPRQLNLHLPDL
HHHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCEEEEECHHH
ASRLDWGQIYKLQPLSDEEKLLALQLRGKLRGFELPEDVGRFLLKRLDREMRTLFMTLDQ
HHHCCCCCEEEECCCCCHHHEEHEEHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
LDHASITAQRKLTIPFVKEILGL
CCCCCCCCCCEEEHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA