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The map label for this gene is menH

Identifier: 157371519

GI number: 157371519

Start: 3625233

End: 3626000

Strand: Reverse

Name: menH

Synonym: Spro_3282

Alternate gene names: 157371519

Gene position: 3626000-3625233 (Counterclockwise)

Preceding gene: 157371520

Following gene: 157371518

Centisome position: 66.55

GC content: 61.07

Gene sequence:

>768_bases
ATGCTGGCGACAAAAGTATTGCAACAGGGCGAAGCCGATCGCCCATGGCTAATCTGGTTACACGGCCTGTTGGGCAATAA
TAATGAATGGCGAGTGATCGCTTCGCGTTGTCCTGAATGGCCGTCGTTGGCCATAGATCTGCCCGGCCACGGCGATTCCG
TTGCGGTCGTCTGCACGAGTTTTGACGATATCAGCGCGCAGATCAGCGCTACTTTGCAGATGCATGGTATCGAACGCTAT
TGGCTGGTGGGGTATTCGCTGGGCGGACGTATTGCCATGTATCACGCCTGTCACGGTGAACCCCGGGGCCTGCAGGGCGT
GATTATCGAAGGTGGCAATCCAGGGCTGGCCGATGCCGCACAGCGCGAACTGCGCTTGGCGCATGATACGGCCTGGGCTG
AGCGTTTCCGCCGAGAACCCCTCGCTCAGGTGCTGGCGGACTGGTATCAACAGCCGGTGTTCGCCAATCTCAGTGCGGTG
CACCGTGATGCGCTGATCGATGCCCGTTCTGACAACGCTGGCGCGGCCGTGGCGGACATGCTCGAAGCCACTTCGCTGGG
CACACAGCCCTGTCTGGCGCCGCAGCTGCAACAACTGGCGGTGCCGCTGGTGGTGTTATGCGGCGCCGACGATCACAAAT
TTCAGCAGCTGACGCGCGACGCCGGGTTGCCGTTGCGCATCGTGCCGCAGGCCGGGCATAACTCGCACCTGGCCAATCCG
CAGGCGTTCGCCGCCGAGCTGCGCAATTTTCTCGTTAACCCTGGTTAA

Upstream 100 bases:

>100_bases
GCTGGCGTCACGGTGGCGCTACGCTGATTGAGCTGCAGGTGCCGCCGAGCGATGGTGCGCAGACGCTGCAGCATCTGGTG
CAACAGATGACGGAGCAATG

Downstream 100 bases:

>100_bases
AGGAACCGAACATGCTCTATCCAAATGAAGAACAGCTGTATGCCGCTATCGAATGGCAGGATTGCTCCGGTGATTTCGAA
GATATTCTGTATCACAAGTC

Product: acyl-CoA thioester hydrolase YfbB

Products: NA

Alternate protein names: SHCHC synthase

Number of amino acids: Translated: 255; Mature: 255

Protein sequence:

>255_residues
MLATKVLQQGEADRPWLIWLHGLLGNNNEWRVIASRCPEWPSLAIDLPGHGDSVAVVCTSFDDISAQISATLQMHGIERY
WLVGYSLGGRIAMYHACHGEPRGLQGVIIEGGNPGLADAAQRELRLAHDTAWAERFRREPLAQVLADWYQQPVFANLSAV
HRDALIDARSDNAGAAVADMLEATSLGTQPCLAPQLQQLAVPLVVLCGADDHKFQQLTRDAGLPLRIVPQAGHNSHLANP
QAFAAELRNFLVNPG

Sequences:

>Translated_255_residues
MLATKVLQQGEADRPWLIWLHGLLGNNNEWRVIASRCPEWPSLAIDLPGHGDSVAVVCTSFDDISAQISATLQMHGIERY
WLVGYSLGGRIAMYHACHGEPRGLQGVIIEGGNPGLADAAQRELRLAHDTAWAERFRREPLAQVLADWYQQPVFANLSAV
HRDALIDARSDNAGAAVADMLEATSLGTQPCLAPQLQQLAVPLVVLCGADDHKFQQLTRDAGLPLRIVPQAGHNSHLANP
QAFAAELRNFLVNPG
>Mature_255_residues
MLATKVLQQGEADRPWLIWLHGLLGNNNEWRVIASRCPEWPSLAIDLPGHGDSVAVVCTSFDDISAQISATLQMHGIERY
WLVGYSLGGRIAMYHACHGEPRGLQGVIIEGGNPGLADAAQRELRLAHDTAWAERFRREPLAQVLADWYQQPVFANLSAV
HRDALIDARSDNAGAAVADMLEATSLGTQPCLAPQLQQLAVPLVVLCGADDHKFQQLTRDAGLPLRIVPQAGHNSHLANP
QAFAAELRNFLVNPG

Specific function: Catalyzes a proton abstraction reaction that results in 2,5-elimination of pyruvate from 2-succinyl-5-enolpyruvyl-6- hydroxy-3-cyclohexene-1-carboxylate (SEPHCHC) and the formation of 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate (SHCHC). Is also

COG id: COG0596

COG function: function code R; Predicted hydrolases or acyltransferases (alpha/beta hydrolase superfamily)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AB hydrolase superfamily. MenH family

Homologues:

Organism=Escherichia coli, GI1788598, Length=244, Percent_Identity=45.4918032786885, Blast_Score=217, Evalue=6e-58,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MENH_SERP5 (A8GGZ0)

Other databases:

- EMBL:   CP000826
- RefSeq:   YP_001479508.1
- ProteinModelPortal:   A8GGZ0
- SMR:   A8GGZ0
- STRING:   A8GGZ0
- GeneID:   5607102
- GenomeReviews:   CP000826_GR
- KEGG:   spe:Spro_3282
- eggNOG:   COG0596
- HOGENOM:   HBG748003
- OMA:   ESCHPGL
- ProtClustDB:   PRK11126
- BioCyc:   SPRO399741:SPRO_3282-MONOMER
- HAMAP:   MF_01660
- InterPro:   IPR000073
- InterPro:   IPR022485
- TIGRFAMs:   TIGR03695

Pfam domain/function: PF00561 Abhydrolase_1

EC number: =4.2.99.20

Molecular weight: Translated: 27751; Mature: 27751

Theoretical pI: Translated: 5.97; Mature: 5.97

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLATKVLQQGEADRPWLIWLHGLLGNNNEWRVIASRCPEWPSLAIDLPGHGDSVAVVCTS
CCHHHHHHCCCCCCCEEEEEEEHHCCCCCEEEECCCCCCCCCEEEECCCCCCCEEEEEEC
FDDISAQISATLQMHGIERYWLVGYSLGGRIAMYHACHGEPRGLQGVIIEGGNPGLADAA
CCCHHHHHHEEEEECCCCEEEEEEECCCCEEEEEEECCCCCCCCEEEEEECCCCCHHHHH
QRELRLAHDTAWAERFRREPLAQVLADWYQQPVFANLSAVHRDALIDARSDNAGAAVADM
HHHHHHHHHHHHHHHHHHCHHHHHHHHHHHCCHHHHHHHHHHHHHEECCCCCCCHHHHHH
LEATSLGTQPCLAPQLQQLAVPLVVLCGADDHKFQQLTRDAGLPLRIVPQAGHNSHLANP
HHHHHCCCCCCCHHHHHHHHCCEEEEECCCCHHHHHHHHHCCCCEEEEECCCCCCCCCCH
QAFAAELRNFLVNPG
HHHHHHHHHHHCCCC
>Mature Secondary Structure
MLATKVLQQGEADRPWLIWLHGLLGNNNEWRVIASRCPEWPSLAIDLPGHGDSVAVVCTS
CCHHHHHHCCCCCCCEEEEEEEHHCCCCCEEEECCCCCCCCCEEEECCCCCCCEEEEEEC
FDDISAQISATLQMHGIERYWLVGYSLGGRIAMYHACHGEPRGLQGVIIEGGNPGLADAA
CCCHHHHHHEEEEECCCCEEEEEEECCCCEEEEEEECCCCCCCCEEEEEECCCCCHHHHH
QRELRLAHDTAWAERFRREPLAQVLADWYQQPVFANLSAVHRDALIDARSDNAGAAVADM
HHHHHHHHHHHHHHHHHHCHHHHHHHHHHHCCHHHHHHHHHHHHHEECCCCCCCHHHHHH
LEATSLGTQPCLAPQLQQLAVPLVVLCGADDHKFQQLTRDAGLPLRIVPQAGHNSHLANP
HHHHHCCCCCCCHHHHHHHHCCEEEEECCCCHHHHHHHHHCCCCEEEEECCCCCCCCCCH
QAFAAELRNFLVNPG
HHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA