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The map label for this gene is rsuA [H]

Identifier: 157371489

GI number: 157371489

Start: 3587173

End: 3587886

Strand: Reverse

Name: rsuA [H]

Synonym: Spro_3252

Alternate gene names: 157371489

Gene position: 3587886-3587173 (Counterclockwise)

Preceding gene: 157371492

Following gene: 157371488

Centisome position: 65.85

GC content: 56.72

Gene sequence:

>714_bases
ATGCGACTGGACAAGTTTTTATCTCAGCAGTTAGGTGTTAGCCGTGCGCTGGTAGCGCGTGAACTTCGGGCAAAACGCGT
CACCGTAGACGGTGAAGTGGTGAAGAGCGGGGCACTGAAACTGACTCCGGAGCAGGAGGTGACGTTTGACGGCAACCCAC
TGCAACGCCAAAACGGCCCGCGCTATTTTATGCTCAATAAACCGCAGGGTTACGTTTGCTCCACGGACGATCCCGATCAT
CCGACGGTGCTGTATTTCCTCGATGAGCCGGTGGCCTACAAGCTGCACGCCGCCGGTCGCCTGGATATCGATACCACCGG
CCTGGTGCTGATGACCGACGACGGCCAATGGTCGCACCGCATCACTTCACCCAAGCATCACTGTGAGAAAACCTATCTGG
TGACGCTGGAGCATCCGCTGGCGGCAGATACCGCGCAGCAATTTGCCGAAGGCGTGCAGTTGCATAACGAAAAAGATCTG
ACCCGACCTGCGCAACTGGAACAAATTGAAGACAAGCTGGTACGCCTGACCATCAGCGAAGGGCGTTATCACCAGGTGAA
ACGCATGTTCGCGGCGGTCGGCAACCGGGTTCTCGAGCTGCATCGCGAACGTATTGGTGCCATCGTGATGGATGAGGATC
TGGCAGAGGGCGAATACCGTCCGCTGACAGAAGAAGAAATCGCCAGCGTTGGTGCTCCGCACCTGCAAGATTAA

Upstream 100 bases:

>100_bases
CCTCTTCGAACCTGCCCTGTTGCACCTGGTGCAACGGTGGGTGTCATATTTTAGCAATACGATCAATGCGATCATAACAA
CGACAGGCAAAGCAGATTCA

Downstream 100 bases:

>100_bases
TTCATTACAAGACTACGGCTGATAAAGGAGTCGTCGAACGTGTTACAGAACCGGTCATCTCACCTCGGTTTGATTTTTAT
TCTGGGTCTGCTTTCCATGC

Product: 16S rRNA pseudouridylate synthase A

Products: pseudouridine 5'-phosphate; H2O [C]

Alternate protein names: 16S pseudouridine 516 synthase; 16S pseudouridylate 516 synthase; rRNA pseudouridylate synthase A; rRNA-uridine isomerase A [H]

Number of amino acids: Translated: 237; Mature: 237

Protein sequence:

>237_residues
MRLDKFLSQQLGVSRALVARELRAKRVTVDGEVVKSGALKLTPEQEVTFDGNPLQRQNGPRYFMLNKPQGYVCSTDDPDH
PTVLYFLDEPVAYKLHAAGRLDIDTTGLVLMTDDGQWSHRITSPKHHCEKTYLVTLEHPLAADTAQQFAEGVQLHNEKDL
TRPAQLEQIEDKLVRLTISEGRYHQVKRMFAAVGNRVLELHRERIGAIVMDEDLAEGEYRPLTEEEIASVGAPHLQD

Sequences:

>Translated_237_residues
MRLDKFLSQQLGVSRALVARELRAKRVTVDGEVVKSGALKLTPEQEVTFDGNPLQRQNGPRYFMLNKPQGYVCSTDDPDH
PTVLYFLDEPVAYKLHAAGRLDIDTTGLVLMTDDGQWSHRITSPKHHCEKTYLVTLEHPLAADTAQQFAEGVQLHNEKDL
TRPAQLEQIEDKLVRLTISEGRYHQVKRMFAAVGNRVLELHRERIGAIVMDEDLAEGEYRPLTEEEIASVGAPHLQD
>Mature_237_residues
MRLDKFLSQQLGVSRALVARELRAKRVTVDGEVVKSGALKLTPEQEVTFDGNPLQRQNGPRYFMLNKPQGYVCSTDDPDH
PTVLYFLDEPVAYKLHAAGRLDIDTTGLVLMTDDGQWSHRITSPKHHCEKTYLVTLEHPLAADTAQQFAEGVQLHNEKDL
TRPAQLEQIEDKLVRLTISEGRYHQVKRMFAAVGNRVLELHRERIGAIVMDEDLAEGEYRPLTEEEIASVGAPHLQD

Specific function: Responsible for synthesis of pseudouridine from uracil- 516 in 16S ribosomal RNA [H]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788510, Length=230, Percent_Identity=81.7391304347826, Blast_Score=400, Evalue=1e-113,
Organism=Escherichia coli, GI1787524, Length=234, Percent_Identity=29.9145299145299, Blast_Score=99, Evalue=2e-22,
Organism=Escherichia coli, GI1790453, Length=229, Percent_Identity=26.2008733624454, Blast_Score=80, Evalue=8e-17,
Organism=Escherichia coli, GI87081838, Length=187, Percent_Identity=32.0855614973262, Blast_Score=76, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496
- InterPro:   IPR002942 [H]

Pfam domain/function: PF00849 PseudoU_synth_2; PF01479 S4 [H]

EC number: 4.2.1.70 [C]

Molecular weight: Translated: 26790; Mature: 26790

Theoretical pI: Translated: 5.79; Mature: 5.79

Prosite motif: PS50889 S4 ; PS01149 PSI_RSU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLDKFLSQQLGVSRALVARELRAKRVTVDGEVVKSGALKLTPEQEVTFDGNPLQRQNGP
CCHHHHHHHHHCHHHHHHHHHHHHHEECCCHHHHCCCCEEECCCCCEEECCCCCCCCCCC
RYFMLNKPQGYVCSTDDPDHPTVLYFLDEPVAYKLHAAGRLDIDTTGLVLMTDDGQWSHR
EEEEEECCCCCEECCCCCCCCEEEEEECCCEEEEEECCCEEEECCCCEEEEECCCCCCCC
ITSPKHHCEKTYLVTLEHPLAADTAQQFAEGVQLHNEKDLTRPAQLEQIEDKLVRLTISE
CCCCHHHCCEEEEEEECCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHEEEEECC
GRYHQVKRMFAAVGNRVLELHRERIGAIVMDEDLAEGEYRPLTEEEIASVGAPHLQD
CHHHHHHHHHHHHHHHHHHHHHHHHCEEEEECCCCCCCCCCCCHHHHHHCCCCCCCC
>Mature Secondary Structure
MRLDKFLSQQLGVSRALVARELRAKRVTVDGEVVKSGALKLTPEQEVTFDGNPLQRQNGP
CCHHHHHHHHHCHHHHHHHHHHHHHEECCCHHHHCCCCEEECCCCCEEECCCCCCCCCCC
RYFMLNKPQGYVCSTDDPDHPTVLYFLDEPVAYKLHAAGRLDIDTTGLVLMTDDGQWSHR
EEEEEECCCCCEECCCCCCCCEEEEEECCCEEEEEECCCEEEECCCCEEEEECCCCCCCC
ITSPKHHCEKTYLVTLEHPLAADTAQQFAEGVQLHNEKDLTRPAQLEQIEDKLVRLTISE
CCCCHHHCCEEEEEEECCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHEEEEECC
GRYHQVKRMFAAVGNRVLELHRERIGAIVMDEDLAEGEYRPLTEEEIASVGAPHLQD
CHHHHHHHHHHHHHHHHHHHHHHHHCEEEEECCCCCCCCCCCCHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose phosphate [C]

Specific reaction: uracil + D-ribose phosphate = pseudouridine 5'-phosphate + H2O [C]

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11586360; 12142430 [H]