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The map label for this gene is bcr [H]

Identifier: 157371488

GI number: 157371488

Start: 3585925

End: 3587133

Strand: Reverse

Name: bcr [H]

Synonym: Spro_3251

Alternate gene names: 157371488

Gene position: 3587133-3585925 (Counterclockwise)

Preceding gene: 157371489

Following gene: 157371487

Centisome position: 65.83

GC content: 56.0

Gene sequence:

>1209_bases
GTGTTACAGAACCGGTCATCTCACCTCGGTTTGATTTTTATTCTGGGTCTGCTTTCCATGCTGATGCCGCTGGCTATCGA
CATGTACCTGCCCAGCATGCCGGTGATTGCCAAACAGTTCGGCGTAGAGTCGGGCAGCGTGCAGATGACGCTCAGCGCCT
ATATGCTCGGCTTTGCCCTGGGGCAGTTGTTCTACGGGCCGATGTCGGACAGCATTGGCCGCAAGCCGGTGATCCTGTGG
GGAACGTTGATCTTCGCCATTGCCGGTGGTGCCTGTGCGATGGCGCAGTCGATCGACCAATTGATCAATCTGCGTTTCCT
GCATGGGCTGTCGGCGGCGGCGGCCAGCGTAGTGATTAATGCCCTGATGCGCGATATGTTCACCAAGGACGAGTTCTCGC
GCATGATGTCGTTTGTCATCCTGGTGATGACCATTGCGCCCTTGCTGGCACCGATGATTGGTGGCGCGTTGCTGCTGTGG
TTCAGTTGGCATGCGATTTTCTGGACCATGGGCGGCGCGGCGCTGATCGGTTCATTGCTGGTGGCGTTCTTTATTAAAGA
GACGCTGCCGAAAGAGCGGCGGCAAAAATTCCATCTGCGTACCACGCTGGGTAACTTCGCTTCCTTGTTCCGCCATAAGC
GCGTGTTGAGCTATATGCTGGCCAGCGCCTTCTCCTTCGCCGGTATGTTCTCGTTCCTCAGCGCCGGGCCGTTCGTGTAT
ATCGAACTGAATCATGTTTCACCGCAGCACTTTGGTTACTATTTTGCGCTGAATATTGTTTTCCTGTTCCTGACCACGCT
GGTTAACAGCCGTAATGTACGGCGGTTGGGCGCGATCAAAATGTTCCGTCTGGGGCTGTTTGTGCAGTTGGCTATGGGGC
TGTGGTTACTGGCGGTGAGCGCTATTGGCCTGGGTTTCTGGGCGTTGGTGTTGGGCGTGGCCGTTTATCTGGGGTGTATC
GCCATGATTTCCTCAAACGCCATGGCAGTGATCCTGGACGACTTCCCGCATATGGCAGGTACGGCATCCTCTCTGGCAGG
TACCTTGCGTTTCAGCATCGGTGCGTTGGTGGGGGCGATACTGGCAATGGCGCCCGGCAAAAGTGCCTGGCCGATGGTTT
CCTCAATGGCGCTGTGTAGCATTGTTGCGGTGTTGTTCTATCTGTATGCCAGTCGGCCACGACAAAAATCCTCGTCTGAT
ACCCCCTAG

Upstream 100 bases:

>100_bases
ATACCGTCCGCTGACAGAAGAAGAAATCGCCAGCGTTGGTGCTCCGCACCTGCAAGATTAATTCATTACAAGACTACGGC
TGATAAAGGAGTCGTCGAAC

Downstream 100 bases:

>100_bases
TCTTTTCTTCCTGATCCAGGCTCAGCGTGATGTTGGGCCTGGGTGAAATATCCCCTCCGGCCCAAGCCCGCTCTGAAACC
CAATGGCAATGAAGACTTCG

Product: bicyclomycin/multidrug efflux system

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: Sulfonamide resistance protein [H]

Number of amino acids: Translated: 402; Mature: 402

Protein sequence:

>402_residues
MLQNRSSHLGLIFILGLLSMLMPLAIDMYLPSMPVIAKQFGVESGSVQMTLSAYMLGFALGQLFYGPMSDSIGRKPVILW
GTLIFAIAGGACAMAQSIDQLINLRFLHGLSAAAASVVINALMRDMFTKDEFSRMMSFVILVMTIAPLLAPMIGGALLLW
FSWHAIFWTMGGAALIGSLLVAFFIKETLPKERRQKFHLRTTLGNFASLFRHKRVLSYMLASAFSFAGMFSFLSAGPFVY
IELNHVSPQHFGYYFALNIVFLFLTTLVNSRNVRRLGAIKMFRLGLFVQLAMGLWLLAVSAIGLGFWALVLGVAVYLGCI
AMISSNAMAVILDDFPHMAGTASSLAGTLRFSIGALVGAILAMAPGKSAWPMVSSMALCSIVAVLFYLYASRPRQKSSSD
TP

Sequences:

>Translated_402_residues
MLQNRSSHLGLIFILGLLSMLMPLAIDMYLPSMPVIAKQFGVESGSVQMTLSAYMLGFALGQLFYGPMSDSIGRKPVILW
GTLIFAIAGGACAMAQSIDQLINLRFLHGLSAAAASVVINALMRDMFTKDEFSRMMSFVILVMTIAPLLAPMIGGALLLW
FSWHAIFWTMGGAALIGSLLVAFFIKETLPKERRQKFHLRTTLGNFASLFRHKRVLSYMLASAFSFAGMFSFLSAGPFVY
IELNHVSPQHFGYYFALNIVFLFLTTLVNSRNVRRLGAIKMFRLGLFVQLAMGLWLLAVSAIGLGFWALVLGVAVYLGCI
AMISSNAMAVILDDFPHMAGTASSLAGTLRFSIGALVGAILAMAPGKSAWPMVSSMALCSIVAVLFYLYASRPRQKSSSD
TP
>Mature_402_residues
MLQNRSSHLGLIFILGLLSMLMPLAIDMYLPSMPVIAKQFGVESGSVQMTLSAYMLGFALGQLFYGPMSDSIGRKPVILW
GTLIFAIAGGACAMAQSIDQLINLRFLHGLSAAAASVVINALMRDMFTKDEFSRMMSFVILVMTIAPLLAPMIGGALLLW
FSWHAIFWTMGGAALIGSLLVAFFIKETLPKERRQKFHLRTTLGNFASLFRHKRVLSYMLASAFSFAGMFSFLSAGPFVY
IELNHVSPQHFGYYFALNIVFLFLTTLVNSRNVRRLGAIKMFRLGLFVQLAMGLWLLAVSAIGLGFWALVLGVAVYLGCI
AMISSNAMAVILDDFPHMAGTASSLAGTLRFSIGALVGAILAMAPGKSAWPMVSSMALCSIVAVLFYLYASRPRQKSSSD
TP

Specific function: Involved in sulfonamide (sulfathiazole) and bicyclomycin resistance. Probable membrane translocase [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Bcr/CmlA family [H]

Homologues:

Organism=Escherichia coli, GI1788509, Length=396, Percent_Identity=68.1818181818182, Blast_Score=549, Evalue=1e-157,
Organism=Escherichia coli, GI48994889, Length=349, Percent_Identity=29.7994269340974, Blast_Score=125, Evalue=5e-30,
Organism=Escherichia coli, GI1790146, Length=391, Percent_Identity=29.156010230179, Blast_Score=117, Evalue=2e-27,
Organism=Escherichia coli, GI87082312, Length=343, Percent_Identity=26.8221574344023, Blast_Score=105, Evalue=7e-24,
Organism=Escherichia coli, GI1787065, Length=259, Percent_Identity=27.4131274131274, Blast_Score=92, Evalue=6e-20,
Organism=Escherichia coli, GI1790794, Length=233, Percent_Identity=25.7510729613734, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI87081738, Length=363, Percent_Identity=24.7933884297521, Blast_Score=65, Evalue=8e-12,
Organism=Escherichia coli, GI1790195, Length=165, Percent_Identity=24.2424242424242, Blast_Score=64, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6324264, Length=187, Percent_Identity=27.807486631016, Blast_Score=72, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6319480, Length=268, Percent_Identity=23.134328358209, Blast_Score=71, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6319517, Length=183, Percent_Identity=25.1366120218579, Blast_Score=66, Evalue=9e-12,
Organism=Saccharomyces cerevisiae, GI6322071, Length=171, Percent_Identity=24.5614035087719, Blast_Score=65, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004812
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR004734 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 43772; Mature: 43772

Theoretical pI: Translated: 10.44; Mature: 10.44

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
6.5 %Met     (Translated Protein)
7.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
6.5 %Met     (Mature Protein)
7.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure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HHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8486276; 9278503; 2694948 [H]