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The map label for this gene is fruK [H]

Identifier: 157371466

GI number: 157371466

Start: 3560299

End: 3561240

Strand: Reverse

Name: fruK [H]

Synonym: Spro_3229

Alternate gene names: 157371466

Gene position: 3561240-3560299 (Counterclockwise)

Preceding gene: 157371467

Following gene: 157371465

Centisome position: 65.36

GC content: 58.6

Gene sequence:

>942_bases
ATGAGCAGAAGAGTAGCAACAATTACCCTGAATCCGGCTTACGATCTGGTGGGCTTCTGCCCGGAGATCGAGCGCGGTGA
AGTTAATCTGGTCAAAACCGCCGGTCTGCACGCCGCCGGAAAAGGCATTAACGTCGCCAAGGTGCTGAAAGATTTGGGCA
TTGATGTCACTGTGGGCGGTTTCCTGGGCAAAGATAACCAGGATGGCTTCCAGCTGCTGTTCAGCGATCTGGGCATTGCC
AACCGCTTCCAGGTGGTACCGGGCCGCACCCGCATTAACGTCAAGCTGACGGAAAAAGACGGTGAAGTGACAGATTTCAA
CTTCTCCGGCTTTGAGGTGAGTGGTCAGGATTGGGAACGTTTCGTGACCGACTCCCTGAGCTGGCTCGGCCAGTTCGATA
TGGTGGCAGTGAGCGGTAGTCTGCCGGCGGGGGTTGATCCCGATGCCTTTACCGACTGGATGATCCGCCTGCGCGCTCAG
TGCCCGTGCATCATTTTCGACAGCAGCCGTGAAGCCTTGGTTGCCGGCTTGAAAGCCGCGCCATGGTTGGTGAAACCCAA
TCGTCGCGAGCTGGAAATCTGGGCCGGTCGCCCGTTGCCACAGTTGGCTGACGTGGTTGAGGCCGCACATGCGCTGCGCG
ATCAGGGCATTGCTCACGTGGTGATTTCTCTCGGTGCCGAAGGGGCGCTGTGGGTCAATGCTTCCGGCGCCTGGATCGCC
AAGCCACCGGCCTGCGAAGTGGTCAGTACCGTTGGTGCCGGTGACTCGATGGTCGGCGGCCTGATTTATGGCCTGCTGAT
GCGCGAATCCAGTGAACATACTTTGCGTTTGGCTACTGCCGTAGCTGCGCTGGCCGTCAGCCAGAGCAACGTTGGCGTGA
CCGACCGTCCACAGTTGGCCGCGATGATGGCGCGTGTCGACCTGAAACCTTTTAATCAATAG

Upstream 100 bases:

>100_bases
AAGGACATCACCTGCGCTTTACCGCCAAGGGTGAAGATGCTGAAACGGCGCTGAAGGCGATCGGTGAGGCCATTAACGAA
GGACTGGGCGAGGGCGCAGC

Downstream 100 bases:

>100_bases
CGGGAGGCAGAATGAAAACACTGCTGATGATAGACAGTTCGCTGGGGCAGGCGCGTGGTCACCTGGCGAAACGCATGCTC
GGCGCTGCTGCAGCGAAGGC

Product: 1-phosphofructokinase

Products: NA

Alternate protein names: Fructose 1-phosphate kinase [H]

Number of amino acids: Translated: 313; Mature: 312

Protein sequence:

>313_residues
MSRRVATITLNPAYDLVGFCPEIERGEVNLVKTAGLHAAGKGINVAKVLKDLGIDVTVGGFLGKDNQDGFQLLFSDLGIA
NRFQVVPGRTRINVKLTEKDGEVTDFNFSGFEVSGQDWERFVTDSLSWLGQFDMVAVSGSLPAGVDPDAFTDWMIRLRAQ
CPCIIFDSSREALVAGLKAAPWLVKPNRRELEIWAGRPLPQLADVVEAAHALRDQGIAHVVISLGAEGALWVNASGAWIA
KPPACEVVSTVGAGDSMVGGLIYGLLMRESSEHTLRLATAVAALAVSQSNVGVTDRPQLAAMMARVDLKPFNQ

Sequences:

>Translated_313_residues
MSRRVATITLNPAYDLVGFCPEIERGEVNLVKTAGLHAAGKGINVAKVLKDLGIDVTVGGFLGKDNQDGFQLLFSDLGIA
NRFQVVPGRTRINVKLTEKDGEVTDFNFSGFEVSGQDWERFVTDSLSWLGQFDMVAVSGSLPAGVDPDAFTDWMIRLRAQ
CPCIIFDSSREALVAGLKAAPWLVKPNRRELEIWAGRPLPQLADVVEAAHALRDQGIAHVVISLGAEGALWVNASGAWIA
KPPACEVVSTVGAGDSMVGGLIYGLLMRESSEHTLRLATAVAALAVSQSNVGVTDRPQLAAMMARVDLKPFNQ
>Mature_312_residues
SRRVATITLNPAYDLVGFCPEIERGEVNLVKTAGLHAAGKGINVAKVLKDLGIDVTVGGFLGKDNQDGFQLLFSDLGIAN
RFQVVPGRTRINVKLTEKDGEVTDFNFSGFEVSGQDWERFVTDSLSWLGQFDMVAVSGSLPAGVDPDAFTDWMIRLRAQC
PCIIFDSSREALVAGLKAAPWLVKPNRRELEIWAGRPLPQLADVVEAAHALRDQGIAHVVISLGAEGALWVNASGAWIAK
PPACEVVSTVGAGDSMVGGLIYGLLMRESSEHTLRLATAVAALAVSQSNVGVTDRPQLAAMMARVDLKPFNQ

Specific function: Unknown

COG id: COG1105

COG function: function code G; Fructose-1-phosphate kinase and related fructose-6-phosphate kinase (PfkB)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the carbohydrate kinase pfkB family [H]

Homologues:

Organism=Escherichia coli, GI1788493, Length=312, Percent_Identity=91.9871794871795, Blast_Score=572, Evalue=1e-164,
Organism=Escherichia coli, GI1788017, Length=286, Percent_Identity=33.5664335664336, Blast_Score=105, Evalue=4e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022463
- InterPro:   IPR011611
- InterPro:   IPR002173
- InterPro:   IPR017583 [H]

Pfam domain/function: PF00294 PfkB [H]

EC number: =2.7.1.56 [H]

Molecular weight: Translated: 33554; Mature: 33423

Theoretical pI: Translated: 5.18; Mature: 5.18

Prosite motif: PS00464 RIBOSOMAL_L22 ; PS00583 PFKB_KINASES_1 ; PS00584 PFKB_KINASES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRRVATITLNPAYDLVGFCPEIERGEVNLVKTAGLHAAGKGINVAKVLKDLGIDVTVGG
CCCEEEEEEECCCHHHHHCCCCCCCCCEEEEEECCCCCCCCCCCHHHHHHHCCCEEEECC
FLGKDNQDGFQLLFSDLGIANRFQVVPGRTRINVKLTEKDGEVTDFNFSGFEVSGQDWER
EECCCCCHHHHHHHHHCCCCCEEEECCCCEEEEEEEECCCCCEEEECCCCEEECCHHHHH
FVTDSLSWLGQFDMVAVSGSLPAGVDPDAFTDWMIRLRAQCPCIIFDSSREALVAGLKAA
HHHHHHHHHCCCEEEEEECCCCCCCCCHHHHHHHHHHHCCCCEEEECCCCHHHHHCCCCC
PWLVKPNRRELEIWAGRPLPQLADVVEAAHALRDQGIAHVVISLGAEGALWVNASGAWIA
CEEECCCCCEEEEECCCCCHHHHHHHHHHHHHHHCCCEEEEEEECCCCEEEEECCCCEEE
KPPACEVVSTVGAGDSMVGGLIYGLLMRESSEHTLRLATAVAALAVSQSNVGVTDRPQLA
CCCCHHHHHHCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHH
AMMARVDLKPFNQ
HHHHHHCCCCCCC
>Mature Secondary Structure 
SRRVATITLNPAYDLVGFCPEIERGEVNLVKTAGLHAAGKGINVAKVLKDLGIDVTVGG
CCEEEEEEECCCHHHHHCCCCCCCCCEEEEEECCCCCCCCCCCHHHHHHHCCCEEEECC
FLGKDNQDGFQLLFSDLGIANRFQVVPGRTRINVKLTEKDGEVTDFNFSGFEVSGQDWER
EECCCCCHHHHHHHHHCCCCCEEEECCCCEEEEEEEECCCCCEEEECCCCEEECCHHHHH
FVTDSLSWLGQFDMVAVSGSLPAGVDPDAFTDWMIRLRAQCPCIIFDSSREALVAGLKAA
HHHHHHHHHCCCEEEEEECCCCCCCCCHHHHHHHHHHHCCCCEEEECCCCHHHHHCCCCC
PWLVKPNRRELEIWAGRPLPQLADVVEAAHALRDQGIAHVVISLGAEGALWVNASGAWIA
CEEECCCCCEEEEECCCCCHHHHHHHHHHHHHHHCCCEEEEEEECCCCEEEEECCCCEEE
KPPACEVVSTVGAGDSMVGGLIYGLLMRESSEHTLRLATAVAALAVSQSNVGVTDRPQLA
CCCCHHHHHHCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHH
AMMARVDLKPFNQ
HHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]