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The map label for this gene is fruB [H]

Identifier: 157371467

GI number: 157371467

Start: 3561237

End: 3562370

Strand: Reverse

Name: fruB [H]

Synonym: Spro_3230

Alternate gene names: 157371467

Gene position: 3562370-3561237 (Counterclockwise)

Preceding gene: 157371472

Following gene: 157371466

Centisome position: 65.38

GC content: 59.52

Gene sequence:

>1134_bases
ATGTTCCAGTTGTCACCGCAAGATATTCACCTGGGCGCAACTGCCGGCAGCAAGCAGGCAGCCATCCAGCAGGTTGCCGC
CGCACTGACCGAAGCCGGCTGCGTCAGCGCCGCCTACGTGGACGGCATGCTGCAGCGCGAGCTGCAAACCTCCACCTATT
TGGGTAACGGCATCGCCATCCCGCACGGCACTACCGATACCCGTGAACTGGTGCTGAATACCGGCGTACAGGTATTCCAG
TTCCCGCAGGGCATTGAGTGGGGCGAAGGCCAAACCGCCTTTGTGGTGATCGGCATTGCCGCCCGCTCCGACGAACACCT
TGCGCTGTTACGCCAGCTGACACACGTGCTGAGCGACGACAGCGTAGCCGAACAGTTGGCGAAAACCACCTCGACAGAAG
AGCTGCGCAGCCTGCTGATGGGCGAAAAGCTGACGGCAGAATTCAAATTTGACGCTTCTCTGATCGCGATCGATGTGGCC
GCCGACAGCCTGATGACGCTGCAGGCACTGAATGCCGGCCGTTTGCAGAAGATCGGTGCGGTCAACGCCCAGTTCGTCAG
CGACGTCATTACCCGCAAGCCGCTGAATTTGGGGCAGGGTATTTGGCTGAGCGACAGCACCGAAGGCAATCTGACCAGCG
CGGCCACTCTGAGCCGTCCGGTGCAGGCGTTTGACGAAGACGGCGAGAAAGTTGCGCTGCTGCTGACGCTGTCGGTGGCT
GACCAGCAACCGCTGGCGGTGCTGAACTACCTGAGTGACCTGCTGTTGGCAAATAAAGCTGAACGCTTGCTGAACGCCGA
TGCCATCACCGTGCTGGCATTGCTGACCAGTGAGGTCGAAGAAGAAAATACCACCCTGAGTGCCGAGTACGTCATTCGTA
ACGAACACGGCCTGCATGCCCGTCCGGGGACGGCGCTGGTCAACGTAATCAAGAAGTTCGAGAGCGAAATTACCGTCACC
AATCTGGATGGCAGTGGCAAACCGGCCAACGGACGCAGCCTGATGAAGGTCGTGGCGTTGGGCGTGAAAAAAGGACATCA
CCTGCGCTTTACCGCCAAGGGTGAAGATGCTGAAACGGCGCTGAAGGCGATCGGTGAGGCCATTAACGAAGGACTGGGCG
AGGGCGCAGCATGA

Upstream 100 bases:

>100_bases
TTGAAACGTTTCAGCGTTATGGGCGCGATGATTCCGTACCGATAACGCTCCTTTTTCCCAATAAAGCTGAAACGATTCAA
TTCAGCAGGAGAGGAAAATC

Downstream 100 bases:

>100_bases
GCAGAAGAGTAGCAACAATTACCCTGAATCCGGCTTACGATCTGGTGGGCTTCTGCCCGGAGATCGAGCGCGGTGAAGTT
AATCTGGTCAAAACCGCCGG

Product: bifunctional PTS system fructose-specific transporter subunit IIA/HPr protein

Products: protein histidine; sugar phosphate

Alternate protein names: MTP; Phosphotransferase FPr protein; Pseudo-HPr; Phosphocarrier protein HPr; Protein H; Fructose-specific phosphotransferase enzyme IIA component; PTS system fructose-specific EIIA component [H]

Number of amino acids: Translated: 377; Mature: 377

Protein sequence:

>377_residues
MFQLSPQDIHLGATAGSKQAAIQQVAAALTEAGCVSAAYVDGMLQRELQTSTYLGNGIAIPHGTTDTRELVLNTGVQVFQ
FPQGIEWGEGQTAFVVIGIAARSDEHLALLRQLTHVLSDDSVAEQLAKTTSTEELRSLLMGEKLTAEFKFDASLIAIDVA
ADSLMTLQALNAGRLQKIGAVNAQFVSDVITRKPLNLGQGIWLSDSTEGNLTSAATLSRPVQAFDEDGEKVALLLTLSVA
DQQPLAVLNYLSDLLLANKAERLLNADAITVLALLTSEVEEENTTLSAEYVIRNEHGLHARPGTALVNVIKKFESEITVT
NLDGSGKPANGRSLMKVVALGVKKGHHLRFTAKGEDAETALKAIGEAINEGLGEGAA

Sequences:

>Translated_377_residues
MFQLSPQDIHLGATAGSKQAAIQQVAAALTEAGCVSAAYVDGMLQRELQTSTYLGNGIAIPHGTTDTRELVLNTGVQVFQ
FPQGIEWGEGQTAFVVIGIAARSDEHLALLRQLTHVLSDDSVAEQLAKTTSTEELRSLLMGEKLTAEFKFDASLIAIDVA
ADSLMTLQALNAGRLQKIGAVNAQFVSDVITRKPLNLGQGIWLSDSTEGNLTSAATLSRPVQAFDEDGEKVALLLTLSVA
DQQPLAVLNYLSDLLLANKAERLLNADAITVLALLTSEVEEENTTLSAEYVIRNEHGLHARPGTALVNVIKKFESEITVT
NLDGSGKPANGRSLMKVVALGVKKGHHLRFTAKGEDAETALKAIGEAINEGLGEGAA
>Mature_377_residues
MFQLSPQDIHLGATAGSKQAAIQQVAAALTEAGCVSAAYVDGMLQRELQTSTYLGNGIAIPHGTTDTRELVLNTGVQVFQ
FPQGIEWGEGQTAFVVIGIAARSDEHLALLRQLTHVLSDDSVAEQLAKTTSTEELRSLLMGEKLTAEFKFDASLIAIDVA
ADSLMTLQALNAGRLQKIGAVNAQFVSDVITRKPLNLGQGIWLSDSTEGNLTSAATLSRPVQAFDEDGEKVALLLTLSVA
DQQPLAVLNYLSDLLLANKAERLLNADAITVLALLTSEVEEENTTLSAEYVIRNEHGLHARPGTALVNVIKKFESEITVT
NLDGSGKPANGRSLMKVVALGVKKGHHLRFTAKGEDAETALKAIGEAINEGLGEGAA

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This

COG id: COG4668

COG function: function code G; Mannitol/fructose-specific phosphotransferase system, IIA domain

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIA type-2 domain [H]

Homologues:

Organism=Escherichia coli, GI1788494, Length=376, Percent_Identity=69.1489361702128, Blast_Score=506, Evalue=1e-145,
Organism=Escherichia coli, GI1790027, Length=145, Percent_Identity=45.5172413793103, Blast_Score=127, Evalue=1e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016152
- InterPro:   IPR002178
- InterPro:   IPR001020
- InterPro:   IPR005698
- InterPro:   IPR000032
- InterPro:   IPR002114 [H]

Pfam domain/function: PF00381 PTS-HPr; PF00359 PTS_EIIA_2 [H]

EC number: 2.7.1.69

Molecular weight: Translated: 39975; Mature: 39975

Theoretical pI: Translated: 4.64; Mature: 4.64

Prosite motif: PS00369 PTS_HPR_HIS ; PS00589 PTS_HPR_SER ; PS00372 PTS_EIIA_TYPE_2_HIS ; PS51094 PTS_EIIA_TYPE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFQLSPQDIHLGATAGSKQAAIQQVAAALTEAGCVSAAYVDGMLQRELQTSTYLGNGIAI
CCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCEEC
PHGTTDTRELVLNTGVQVFQFPQGIEWGEGQTAFVVIGIAARSDEHLALLRQLTHVLSDD
CCCCCHHHHHHHHCCCCEEECCCCCCCCCCCEEEEEEEEECCCCHHHHHHHHHHHHHCCH
SVAEQLAKTTSTEELRSLLMGEKLTAEFKFDASLIAIDVAADSLMTLQALNAGRLQKIGA
HHHHHHHHCCCHHHHHHHHHCCHHCEEEEECCEEEEEEEHHHHHHHHHHCCCCHHHHHCC
VNAQFVSDVITRKPLNLGQGIWLSDSTEGNLTSAATLSRPVQAFDEDGEKVALLLTLSVA
CHHHHHHHHHHCCCCCCCCCEEECCCCCCCCCHHHHHHHHHHHHCCCCCEEEEEEEEECC
DQQPLAVLNYLSDLLLANKAERLLNADAITVLALLTSEVEEENTTLSAEYVIRNEHGLHA
CCCHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCEEEEEEEEECCCCCCC
RPGTALVNVIKKFESEITVTNLDGSGKPANGRSLMKVVALGVKKGHHLRFTAKGEDAETA
CCHHHHHHHHHHHHCCEEEEECCCCCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCHHHH
LKAIGEAINEGLGEGAA
HHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MFQLSPQDIHLGATAGSKQAAIQQVAAALTEAGCVSAAYVDGMLQRELQTSTYLGNGIAI
CCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCEEC
PHGTTDTRELVLNTGVQVFQFPQGIEWGEGQTAFVVIGIAARSDEHLALLRQLTHVLSDD
CCCCCHHHHHHHHCCCCEEECCCCCCCCCCCEEEEEEEEECCCCHHHHHHHHHHHHHCCH
SVAEQLAKTTSTEELRSLLMGEKLTAEFKFDASLIAIDVAADSLMTLQALNAGRLQKIGA
HHHHHHHHCCCHHHHHHHHHCCHHCEEEEECCEEEEEEEHHHHHHHHHHCCCCHHHHHCC
VNAQFVSDVITRKPLNLGQGIWLSDSTEGNLTSAATLSRPVQAFDEDGEKVALLLTLSVA
CHHHHHHHHHHCCCCCCCCCEEECCCCCCCCCHHHHHHHHHHHHCCCCCEEEEEEEEECC
DQQPLAVLNYLSDLLLANKAERLLNADAITVLALLTSEVEEENTTLSAEYVIRNEHGLHA
CCCHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCEEEEEEEEECCCCCCC
RPGTALVNVIKKFESEITVTNLDGSGKPANGRSLMKVVALGVKKGHHLRFTAKGEDAETA
CCHHHHHHHHHHHHCCEEEEECCCCCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCHHHH
LKAIGEAINEGLGEGAA
HHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: protein N(pi)-phosphohistidine; sugar

Specific reaction: protein N(pi)-phosphohistidine + sugar = protein histidine + sugar phosphate

General reaction: Transferring phosphorus-containing groups [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]