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The map label for this gene is sotB [H]

Identifier: 157371431

GI number: 157371431

Start: 3517489

End: 3518748

Strand: Reverse

Name: sotB [H]

Synonym: Spro_3194

Alternate gene names: 157371431

Gene position: 3518748-3517489 (Counterclockwise)

Preceding gene: 157371432

Following gene: 157371430

Centisome position: 64.58

GC content: 57.38

Gene sequence:

>1260_bases
ATGTCGCATTTACCCTGCCGATTGGTTAAAATATCGCTACTTCCCGCTCCCGTCCGGATCCCGTTTAACATGGAACAAAC
TCAGGTCTCTCGCTCAACTGCCTGGTTGCGCGTGGTCATTCTTGCCGTTTCGGCCTTTATCTTCAATACCACCGAGTTCA
TTCCGGTCGGGTTGCTGAGTGATATCGCCCAAAGCTTCTCGATGCAGACCGAGCAGGTGGGGTTGATCATCACTATTTAT
GCCTGGATCGTCGCCGCCAGTTCGCTGGTGTGTATGCTGCTGACCAGCCGGATTGAGCGGCGCAAACTGCTGATCGGGGT
ATTTATCCTGTTTATCGCCAGCCATGTGCTGACCGCCGTGGCATGGAACTTCACCACGCTGGTGATTTCCCGTGCCGGTG
TGGCGCTGGCCCATGCGGTGTTTTGGTCGATCACCGCCTCGCTGGCGATCCGGGTCGCACCCGCGGGTAAAAAGGCGCAG
GCATTGAGCCTGTTGGCGGGGGGAACCGCGCTGGCGATGGTGCTCGGCCTGCCGCTGGGCCGTATCGTGGGTCAAATCCT
CGGCTGGCGCATGACCTTTATTGGTATCGCCGTCTGTGCCACGCTGGCGTTGGTGCTGCTGTGGCGTTTGTTGCCGGTGC
TGAAAAGCGAGCATTCCGGCTCGCTGGCCAGCGTGCCAATGCTGTTTAAGCGCCCGGCGTTGGTCAGCCTGTATATGCTG
ACCATCATTGTGGTGACGGCCAATTTTACCGCCTACAGCTATATCGAACCCTTTATTCAGACCGTGGCCGGGCTGTCGGA
AAACTTCACCACCCTGATGCTGTTGCTGTTCGGTGCCGCCGGTATCATCGGCAGTCTGCTGTTTAGCCGTTTCAGCGAGC
GTTTCCCGTCGGGCTTTTTTATCGGCGCCATCGCTTTATTGATGCTGAGCCTGCTGTTGCTGTTACCGGCCTCCGGTGAT
GAAAACCACCTGACGGTGCTGTTTGCGGTATGGGGCATGGCAATCATGGCAATTGGTTTGTCGATGCAGGCCAAAGTATT
GAACCTGGCCCCGGACGCTACCGACGTGGCGATGTCGATCTATTCCGGGCTGTATAACTTCGGTATCGGTGCCGGCGCAC
TGCTGGGTAACCAGGTCAGCCAGTATCTGGGTATGGGCAACATTGGCTTTGTCGCTGCCCCGCTGGCGTTGATCGCCCTT
GGCTGGTGCCTGCTGAACTTTTACCGTAGTGAACGCCTGCAACACCACCAAATCCGCTAA

Upstream 100 bases:

>100_bases
AGTCAGTAAACGGCATCCGTTGCTGTCCAGGGTCTGCCGTTGTGCAGGCCGTGGATGGCAAAAGGCTGGCGAAAAAAACA
GGTTAATAAAAATGTGACGC

Downstream 100 bases:

>100_bases
CTTCCTGCGCTCTGATAGTGGGCCGCCGGCTCACTGTCAGGCATCTTCCCACGGCTTAAATTTTCTCTCAGCAAAACAGA
TTGCTGCAGTTTTTTATGCT

Product: sugar efflux transporter

Products: arabinose [Periplasm]; Proton [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 419; Mature: 418

Protein sequence:

>419_residues
MSHLPCRLVKISLLPAPVRIPFNMEQTQVSRSTAWLRVVILAVSAFIFNTTEFIPVGLLSDIAQSFSMQTEQVGLIITIY
AWIVAASSLVCMLLTSRIERRKLLIGVFILFIASHVLTAVAWNFTTLVISRAGVALAHAVFWSITASLAIRVAPAGKKAQ
ALSLLAGGTALAMVLGLPLGRIVGQILGWRMTFIGIAVCATLALVLLWRLLPVLKSEHSGSLASVPMLFKRPALVSLYML
TIIVVTANFTAYSYIEPFIQTVAGLSENFTTLMLLLFGAAGIIGSLLFSRFSERFPSGFFIGAIALLMLSLLLLLPASGD
ENHLTVLFAVWGMAIMAIGLSMQAKVLNLAPDATDVAMSIYSGLYNFGIGAGALLGNQVSQYLGMGNIGFVAAPLALIAL
GWCLLNFYRSERLQHHQIR

Sequences:

>Translated_419_residues
MSHLPCRLVKISLLPAPVRIPFNMEQTQVSRSTAWLRVVILAVSAFIFNTTEFIPVGLLSDIAQSFSMQTEQVGLIITIY
AWIVAASSLVCMLLTSRIERRKLLIGVFILFIASHVLTAVAWNFTTLVISRAGVALAHAVFWSITASLAIRVAPAGKKAQ
ALSLLAGGTALAMVLGLPLGRIVGQILGWRMTFIGIAVCATLALVLLWRLLPVLKSEHSGSLASVPMLFKRPALVSLYML
TIIVVTANFTAYSYIEPFIQTVAGLSENFTTLMLLLFGAAGIIGSLLFSRFSERFPSGFFIGAIALLMLSLLLLLPASGD
ENHLTVLFAVWGMAIMAIGLSMQAKVLNLAPDATDVAMSIYSGLYNFGIGAGALLGNQVSQYLGMGNIGFVAAPLALIAL
GWCLLNFYRSERLQHHQIR
>Mature_418_residues
SHLPCRLVKISLLPAPVRIPFNMEQTQVSRSTAWLRVVILAVSAFIFNTTEFIPVGLLSDIAQSFSMQTEQVGLIITIYA
WIVAASSLVCMLLTSRIERRKLLIGVFILFIASHVLTAVAWNFTTLVISRAGVALAHAVFWSITASLAIRVAPAGKKAQA
LSLLAGGTALAMVLGLPLGRIVGQILGWRMTFIGIAVCATLALVLLWRLLPVLKSEHSGSLASVPMLFKRPALVSLYMLT
IIVVTANFTAYSYIEPFIQTVAGLSENFTTLMLLLFGAAGIIGSLLFSRFSERFPSGFFIGAIALLMLSLLLLLPASGDE
NHLTVLFAVWGMAIMAIGLSMQAKVLNLAPDATDVAMSIYSGLYNFGIGAGALLGNQVSQYLGMGNIGFVAAPLALIALG
WCLLNFYRSERLQHHQIR

Specific function: Involved in the efflux of sugars. The physiological role may be the reduction of the intracellular concentration of toxic sugars or sugar metabolites. Transports IPTG, lactose and melibiose [H]

COG id: COG2814

COG function: function code G; Arabinose efflux permease

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. SotB (TC 2.A.1.2) family [H]

Homologues:

Organism=Escherichia coli, GI1787808, Length=386, Percent_Identity=65.2849740932643, Blast_Score=437, Evalue=1e-124,
Organism=Escherichia coli, GI1787947, Length=325, Percent_Identity=30.7692307692308, Blast_Score=143, Evalue=2e-35,
Organism=Escherichia coli, GI145693198, Length=334, Percent_Identity=28.4431137724551, Blast_Score=124, Evalue=1e-29,
Organism=Escherichia coli, GI1786595, Length=380, Percent_Identity=25.5263157894737, Blast_Score=103, Evalue=3e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 45185; Mature: 45054

Theoretical pI: Translated: 10.22; Mature: 10.22

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSHLPCRLVKISLLPAPVRIPFNMEQTQVSRSTAWLRVVILAVSAFIFNTTEFIPVGLLS
CCCCCCCCEEEEECCCCEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
DIAQSFSMQTEQVGLIITIYAWIVAASSLVCMLLTSRIERRKLLIGVFILFIASHVLTAV
HHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AWNFTTLVISRAGVALAHAVFWSITASLAIRVAPAGKKAQALSLLAGGTALAMVLGLPLG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCHHHHHHHHHHHHHHHHHHHCCHH
RIVGQILGWRMTFIGIAVCATLALVLLWRLLPVLKSEHSGSLASVPMLFKRPALVSLYML
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHCHHHHHCHHHHHHHHH
TIIVVTANFTAYSYIEPFIQTVAGLSENFTTLMLLLFGAAGIIGSLLFSRFSERFPSGFF
HHHHHHCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHH
IGAIALLMLSLLLLLPASGDENHLTVLFAVWGMAIMAIGLSMQAKVLNLAPDATDVAMSI
HHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCHHHHHHHH
YSGLYNFGIGAGALLGNQVSQYLGMGNIGFVAAPLALIALGWCLLNFYRSERLQHHQIR
HHHHHHHCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SHLPCRLVKISLLPAPVRIPFNMEQTQVSRSTAWLRVVILAVSAFIFNTTEFIPVGLLS
CCCCCCCEEEEECCCCEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
DIAQSFSMQTEQVGLIITIYAWIVAASSLVCMLLTSRIERRKLLIGVFILFIASHVLTAV
HHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AWNFTTLVISRAGVALAHAVFWSITASLAIRVAPAGKKAQALSLLAGGTALAMVLGLPLG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCHHHHHHHHHHHHHHHHHHHCCHH
RIVGQILGWRMTFIGIAVCATLALVLLWRLLPVLKSEHSGSLASVPMLFKRPALVSLYML
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHCHHHHHCHHHHHHHHH
TIIVVTANFTAYSYIEPFIQTVAGLSENFTTLMLLLFGAAGIIGSLLFSRFSERFPSGFF
HHHHHHCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHH
IGAIALLMLSLLLLLPASGDENHLTVLFAVWGMAIMAIGLSMQAKVLNLAPDATDVAMSI
HHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCHHHHHHHH
YSGLYNFGIGAGALLGNQVSQYLGMGNIGFVAAPLALIALGWCLLNFYRSERLQHHQIR
HHHHHHHCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: arabinose [Cytoplasm]; Proton [Periplasm] [C]

Specific reaction: arabinose [Cytoplasm] + Proton [Periplasm] = arabinose [Periplasm] + Proton [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 10671456 [H]