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The map label for this gene is ybiW [H]

Identifier: 157370882

GI number: 157370882

Start: 2910614

End: 2913046

Strand: Direct

Name: ybiW [H]

Synonym: Spro_2642

Alternate gene names: 157370882

Gene position: 2910614-2913046 (Clockwise)

Preceding gene: 157370881

Following gene: 157370883

Centisome position: 53.42

GC content: 57.71

Gene sequence:

>2433_bases
ATGACCACGCTGGACCTGACCACCCTGACCGCACGCACCCTGGCCCACAAGAACGCGTTGATCCATATCGTCAAACCGCC
GGTTTGCACCGAACGCGCACAACACTATACCGAAGCCTATCAGCAACACCTGGACAAACCCTTGCCGGTGCGTCGGGCGC
TGGCATTAGCTAATCATCTGGCCAAACGTACCCTGAGAATCGACAACGATGAGCTGATTATCGGCAACCAGGCCAGCGAA
TTACGCGCCGCGCCGATCTTCCCGGAATATACCGTCAGTTGGATCGAAGACGAGATTGACCAGTTGGCCGACCGTCCGGG
TGCCGGTTTTTCCATCAGCGAAGAGAATAAGGCCATCCTGCACCGGATATGCCCGTGGTGGCGTGGCCAGACGGTACAAG
ACCGCTGCTACGGCATGTTCACCGATGAACAACGGGCGCTGCTGGCCACCGGCATCATCAAGGCCGAAGGCAATATGACT
TCGGGAGATGCCCACCTGGCGGTGAATTTCCCACTGCTGTTGGAGAAAGGCCTCGACGGACTACGCGACAAGGTGGACGA
GCGCCGCAGCCGCATTCACCTCACCGACTGGCAAGAGTTACAGCAGGAACAATTCCTGAAGGCTATCGACCTGACCCTGG
AGGCGCTGAGCGCCCATATCCTGCGCTTTGCCGAACTGGCCCGCAGCATGGCACAGGCGGAAAGCCGCCACTGGCGGAGT
GAAGAGCTGCAGGCCATCGCGGAAAACTGCGAGTTGATCGCCCACCGCCCACCGCAGACCTTCTGGCAGGCGCTGCAACT
GTGTTACTTCATTCAGTTAACGCTACAAATTGAATCCAACGGCCATTCGGTGTCCTTTGGCCGTCTCGACCAATATCTGT
ACCCCTGGTATCGCCGCGATGTCGAGCTGGAGCAGACTCTGCCACGTGAGCGTGCGATTGAAATGCTGCACAGCTGCTGG
CTGAAACTTTTGGAGATCAACAAGATCCGTTCCGGCTCGCACTCCAAAGCCTCGGCAGGCAGCCCGCTGTATCAGAACGT
CACTATCGGCGGACAACAGCTGGTCGATGGCTCACCCCGCGACGCGGTGAACCCTTTGTCCTATACCATTCTGGAGTCCT
GCGGTCGCCTGCGTTCGACCCAACCCAACCTCAGCGTTCGTTACCATGCCGGGATGAGCAGCGACTTTCTCGATGCATGC
GTGCAGGTGATCCGCTGCGGGTTTGGCATGCCGGCCTTCAACAACGATGAGATAGTGATTCCGGAGTTTATCAAGCTGGG
CGTGGAACCTCAGGATGCCTATGATTATGCGGCGATCGGCTGCATCGAAACCGCCGTCGGTGGGAAATGGGGTTATCGCT
GCACCGGCATGAGCTTTATCAACTTCGCCCGAGTATTGCTGGCAGCACTGGAGCAAGGGCGTGATGCCACCTCCGGTAAA
GTCTTCCTGCCCCAGGAGTTGGCGTTGTCGCGGGGCAACTTCAGCCATTTCGACCAGGTGCTGGGTGCCTGGGACAGCCA
GATCCGCTACTACACCCGTAAGTCGATTGAAATCGAGTGCGTGGTCGATAGCGTGCTGGAAGAAAATGCTCATGACATCC
TGTGTTCAGCGCTGGTGGACGACTGCATCGAACGCGGTAAAAGCATTAAGCAAGGCGGTGCAAAATACGACTGGGTTTCG
GGCCTGCAGGTCGGTATCGCCAACCTGGGCAACAGCCTGGCGGCAGTGCGTAAACTGGTGTTCGAACAGGGTGCGATCGG
CCAACAGCAGTTGGCGGCAGCGCTGGCCAATGACTTTGACGGGCTGAGCGGCGAGCAGTTACGTCAGCGGTTGCTCAACG
CCGCGCCAAAATACGGCAATGACGTGGATGAGGTCGACCAATTGCTGGTACGCGCTTATCAAAGCTACATTGACGAACTC
GCGCAGTACCACAATACCCGCTTTGGTCGTGGCCCGATTGGCGGCACTTACTACGCCGGCACTTCGTCCATTTCGGCTAA
CGTCCCCTTTGGCGCAGCTACCCTGGCAACCCCTGATGGGCGCAAAGCGCATACGCCGCTGGCGGAAGGTGCCAGCCCGG
CATCAGGTACCGACCATCTCGGGCCGACTGCCGTGTTCAATTCACTGTCCAAGTTGCCTACCGCTGCAATCCTCGGTGGC
GTGCTGCTGAACCAGAAACTCAACCCGGCCACGCTGGAAAACCCGGTCGACCGTGAAAAGCTGATGTTGATGCTGCGCAC
CTTCTTTGAGACCTACCTGGGCTGGCACGTACAGTACAACATTGTGTCGCGGGAAACCCTGCTGGAAGCGAAGCAACATC
CTGACCAGTATCGTGATTTAGTGGTTCGCGTTGCTGGATATTCCGCGTTCTTTACCGCATTATCCCCTGACGCGCAGGAT
GATATTATTGCGCGAACAGAACATACTATTTAA

Upstream 100 bases:

>100_bases
CACGCTGCCCGTACCCCACTGGATGCGCCCGAACTGCTGGCCTATGCCGAGCAGTACGCCAACACCAAAGGGCTGACCGC
TATTTTGCGAGGATAACATC

Downstream 100 bases:

>100_bases
ACAACAAATATTAGCGGCCGCACCTTGGTGGCCGCACATCAGGTTGATATGAATTCAAGACAACAGACAATATTGCAATT
GGTTAACGATCGTCGGCGCA

Product: pyruvate formate-lyase

Products: NA

Alternate protein names: Pyruvate formate-lyase 3 [H]

Number of amino acids: Translated: 810; Mature: 809

Protein sequence:

>810_residues
MTTLDLTTLTARTLAHKNALIHIVKPPVCTERAQHYTEAYQQHLDKPLPVRRALALANHLAKRTLRIDNDELIIGNQASE
LRAAPIFPEYTVSWIEDEIDQLADRPGAGFSISEENKAILHRICPWWRGQTVQDRCYGMFTDEQRALLATGIIKAEGNMT
SGDAHLAVNFPLLLEKGLDGLRDKVDERRSRIHLTDWQELQQEQFLKAIDLTLEALSAHILRFAELARSMAQAESRHWRS
EELQAIAENCELIAHRPPQTFWQALQLCYFIQLTLQIESNGHSVSFGRLDQYLYPWYRRDVELEQTLPRERAIEMLHSCW
LKLLEINKIRSGSHSKASAGSPLYQNVTIGGQQLVDGSPRDAVNPLSYTILESCGRLRSTQPNLSVRYHAGMSSDFLDAC
VQVIRCGFGMPAFNNDEIVIPEFIKLGVEPQDAYDYAAIGCIETAVGGKWGYRCTGMSFINFARVLLAALEQGRDATSGK
VFLPQELALSRGNFSHFDQVLGAWDSQIRYYTRKSIEIECVVDSVLEENAHDILCSALVDDCIERGKSIKQGGAKYDWVS
GLQVGIANLGNSLAAVRKLVFEQGAIGQQQLAAALANDFDGLSGEQLRQRLLNAAPKYGNDVDEVDQLLVRAYQSYIDEL
AQYHNTRFGRGPIGGTYYAGTSSISANVPFGAATLATPDGRKAHTPLAEGASPASGTDHLGPTAVFNSLSKLPTAAILGG
VLLNQKLNPATLENPVDREKLMLMLRTFFETYLGWHVQYNIVSRETLLEAKQHPDQYRDLVVRVAGYSAFFTALSPDAQD
DIIARTEHTI

Sequences:

>Translated_810_residues
MTTLDLTTLTARTLAHKNALIHIVKPPVCTERAQHYTEAYQQHLDKPLPVRRALALANHLAKRTLRIDNDELIIGNQASE
LRAAPIFPEYTVSWIEDEIDQLADRPGAGFSISEENKAILHRICPWWRGQTVQDRCYGMFTDEQRALLATGIIKAEGNMT
SGDAHLAVNFPLLLEKGLDGLRDKVDERRSRIHLTDWQELQQEQFLKAIDLTLEALSAHILRFAELARSMAQAESRHWRS
EELQAIAENCELIAHRPPQTFWQALQLCYFIQLTLQIESNGHSVSFGRLDQYLYPWYRRDVELEQTLPRERAIEMLHSCW
LKLLEINKIRSGSHSKASAGSPLYQNVTIGGQQLVDGSPRDAVNPLSYTILESCGRLRSTQPNLSVRYHAGMSSDFLDAC
VQVIRCGFGMPAFNNDEIVIPEFIKLGVEPQDAYDYAAIGCIETAVGGKWGYRCTGMSFINFARVLLAALEQGRDATSGK
VFLPQELALSRGNFSHFDQVLGAWDSQIRYYTRKSIEIECVVDSVLEENAHDILCSALVDDCIERGKSIKQGGAKYDWVS
GLQVGIANLGNSLAAVRKLVFEQGAIGQQQLAAALANDFDGLSGEQLRQRLLNAAPKYGNDVDEVDQLLVRAYQSYIDEL
AQYHNTRFGRGPIGGTYYAGTSSISANVPFGAATLATPDGRKAHTPLAEGASPASGTDHLGPTAVFNSLSKLPTAAILGG
VLLNQKLNPATLENPVDREKLMLMLRTFFETYLGWHVQYNIVSRETLLEAKQHPDQYRDLVVRVAGYSAFFTALSPDAQD
DIIARTEHTI
>Mature_809_residues
TTLDLTTLTARTLAHKNALIHIVKPPVCTERAQHYTEAYQQHLDKPLPVRRALALANHLAKRTLRIDNDELIIGNQASEL
RAAPIFPEYTVSWIEDEIDQLADRPGAGFSISEENKAILHRICPWWRGQTVQDRCYGMFTDEQRALLATGIIKAEGNMTS
GDAHLAVNFPLLLEKGLDGLRDKVDERRSRIHLTDWQELQQEQFLKAIDLTLEALSAHILRFAELARSMAQAESRHWRSE
ELQAIAENCELIAHRPPQTFWQALQLCYFIQLTLQIESNGHSVSFGRLDQYLYPWYRRDVELEQTLPRERAIEMLHSCWL
KLLEINKIRSGSHSKASAGSPLYQNVTIGGQQLVDGSPRDAVNPLSYTILESCGRLRSTQPNLSVRYHAGMSSDFLDACV
QVIRCGFGMPAFNNDEIVIPEFIKLGVEPQDAYDYAAIGCIETAVGGKWGYRCTGMSFINFARVLLAALEQGRDATSGKV
FLPQELALSRGNFSHFDQVLGAWDSQIRYYTRKSIEIECVVDSVLEENAHDILCSALVDDCIERGKSIKQGGAKYDWVSG
LQVGIANLGNSLAAVRKLVFEQGAIGQQQLAAALANDFDGLSGEQLRQRLLNAAPKYGNDVDEVDQLLVRAYQSYIDELA
QYHNTRFGRGPIGGTYYAGTSSISANVPFGAATLATPDGRKAHTPLAEGASPASGTDHLGPTAVFNSLSKLPTAAILGGV
LLNQKLNPATLENPVDREKLMLMLRTFFETYLGWHVQYNIVSRETLLEAKQHPDQYRDLVVRVAGYSAFFTALSPDAQDD
IIARTEHTI

Specific function: Glucose metabolism (nonoxidative conversion). [C]

COG id: COG1882

COG function: function code C; Pyruvate-formate lyase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 pyruvate formate lyase domain [H]

Homologues:

Organism=Escherichia coli, GI1787044, Length=810, Percent_Identity=85.1851851851852, Blast_Score=1456, Evalue=0.0,
Organism=Escherichia coli, GI1790388, Length=806, Percent_Identity=37.3449131513648, Blast_Score=469, Evalue=1e-133,
Organism=Escherichia coli, GI48994926, Length=589, Percent_Identity=26.9949066213922, Blast_Score=167, Evalue=2e-42,
Organism=Escherichia coli, GI1787131, Length=561, Percent_Identity=25.3119429590018, Blast_Score=159, Evalue=8e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001150
- InterPro:   IPR019777
- InterPro:   IPR004184
- InterPro:   IPR010098 [H]

Pfam domain/function: PF01228 Gly_radical; PF02901 PFL [H]

EC number: =2.3.1.54 [H]

Molecular weight: Translated: 90196; Mature: 90065

Theoretical pI: Translated: 5.95; Mature: 5.95

Prosite motif: PS00850 GLY_RADICAL_1 ; PS51149 GLY_RADICAL_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTLDLTTLTARTLAHKNALIHIVKPPVCTERAQHYTEAYQQHLDKPLPVRRALALANHL
CCCEEHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
AKRTLRIDNDELIIGNQASELRAAPIFPEYTVSWIEDEIDQLADRPGAGFSISEENKAIL
HHHHEECCCCCEEECCCHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHH
HRICPWWRGQTVQDRCYGMFTDEQRALLATGIIKAEGNMTSGDAHLAVNFPLLLEKGLDG
HHHCCCCCCCCHHHHHCCCCCCCCHHHHHHHEEEECCCCCCCCCEEEEECHHHHHHCCHH
LRDKVDERRSRIHLTDWQELQQEQFLKAIDLTLEALSAHILRFAELARSMAQAESRHWRS
HHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
EELQAIAENCELIAHRPPQTFWQALQLCYFIQLTLQIESNGHSVSFGRLDQYLYPWYRRD
HHHHHHHHCCCEEECCCHHHHHHHHHHHHHHHEEEEEECCCCEEEHHHHHHHHHHHHHCC
VELEQTLPRERAIEMLHSCWLKLLEINKIRSGSHSKASAGSPLYQNVTIGGQQLVDGSPR
CCHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEECCCHHHCCCCCC
DAVNPLSYTILESCGRLRSTQPNLSVRYHAGMSSDFLDACVQVIRCGFGMPAFNNDEIVI
CCCCHHHHHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHHHHHCCCCCCCCCCCEEC
PEFIKLGVEPQDAYDYAAIGCIETAVGGKWGYRCTGMSFINFARVLLAALEQGRDATSGK
HHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCEECCHHHHHHHHHHHHHHHCCCCCCCCE
VFLPQELALSRGNFSHFDQVLGAWDSQIRYYTRKSIEIECVVDSVLEENAHDILCSALVD
EECCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEHHHHHHHHCCHHHHHHHHHHH
DCIERGKSIKQGGAKYDWVSGLQVGIANLGNSLAAVRKLVFEQGAIGQQQLAAALANDFD
HHHHCCCHHHCCCCCCHHHHHHHHHHHHHCHHHHHHHHHHHHCCCCCHHHHHHHHHHCCC
GLSGEQLRQRLLNAAPKYGNDVDEVDQLLVRAYQSYIDELAQYHNTRFGRGPIGGTYYAG
CCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEECC
TSSISANVPFGAATLATPDGRKAHTPLAEGASPASGTDHLGPTAVFNSLSKLPTAAILGG
CCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCHHHHHHH
VLLNQKLNPATLENPVDREKLMLMLRTFFETYLGWHVQYNIVSRETLLEAKQHPDQYRDL
HHHCCCCCCHHCCCCCCHHHHHHHHHHHHHHHCCCEEEEEEHHHHHHHHHHHCCHHHHHH
VVRVAGYSAFFTALSPDAQDDIIARTEHTI
HHHHHHHHHHHHHCCCCCCCCHHHHCCCCC
>Mature Secondary Structure 
TTLDLTTLTARTLAHKNALIHIVKPPVCTERAQHYTEAYQQHLDKPLPVRRALALANHL
CCEEHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
AKRTLRIDNDELIIGNQASELRAAPIFPEYTVSWIEDEIDQLADRPGAGFSISEENKAIL
HHHHEECCCCCEEECCCHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHH
HRICPWWRGQTVQDRCYGMFTDEQRALLATGIIKAEGNMTSGDAHLAVNFPLLLEKGLDG
HHHCCCCCCCCHHHHHCCCCCCCCHHHHHHHEEEECCCCCCCCCEEEEECHHHHHHCCHH
LRDKVDERRSRIHLTDWQELQQEQFLKAIDLTLEALSAHILRFAELARSMAQAESRHWRS
HHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
EELQAIAENCELIAHRPPQTFWQALQLCYFIQLTLQIESNGHSVSFGRLDQYLYPWYRRD
HHHHHHHHCCCEEECCCHHHHHHHHHHHHHHHEEEEEECCCCEEEHHHHHHHHHHHHHCC
VELEQTLPRERAIEMLHSCWLKLLEINKIRSGSHSKASAGSPLYQNVTIGGQQLVDGSPR
CCHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEECCCHHHCCCCCC
DAVNPLSYTILESCGRLRSTQPNLSVRYHAGMSSDFLDACVQVIRCGFGMPAFNNDEIVI
CCCCHHHHHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHHHHHCCCCCCCCCCCEEC
PEFIKLGVEPQDAYDYAAIGCIETAVGGKWGYRCTGMSFINFARVLLAALEQGRDATSGK
HHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCEECCHHHHHHHHHHHHHHHCCCCCCCCE
VFLPQELALSRGNFSHFDQVLGAWDSQIRYYTRKSIEIECVVDSVLEENAHDILCSALVD
EECCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEHHHHHHHHCCHHHHHHHHHHH
DCIERGKSIKQGGAKYDWVSGLQVGIANLGNSLAAVRKLVFEQGAIGQQQLAAALANDFD
HHHHCCCHHHCCCCCCHHHHHHHHHHHHHCHHHHHHHHHHHHCCCCCHHHHHHHHHHCCC
GLSGEQLRQRLLNAAPKYGNDVDEVDQLLVRAYQSYIDELAQYHNTRFGRGPIGGTYYAG
CCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEECC
TSSISANVPFGAATLATPDGRKAHTPLAEGASPASGTDHLGPTAVFNSLSKLPTAAILGG
CCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCHHHHHHH
VLLNQKLNPATLENPVDREKLMLMLRTFFETYLGWHVQYNIVSRETLLEAKQHPDQYRDL
HHHCCCCCCHHCCCCCCHHHHHHHHHHHHHHHCCCEEEEEEHHHHHHHHHHHCCHHHHHH
VVRVAGYSAFFTALSPDAQDDIIARTEHTI
HHHHHHHHHHHHHCCCCCCCCHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503 [H]