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The map label for this gene is ybiY [H]

Identifier: 157370881

GI number: 157370881

Start: 2909710

End: 2910609

Strand: Direct

Name: ybiY [H]

Synonym: Spro_2641

Alternate gene names: 157370881

Gene position: 2909710-2910609 (Clockwise)

Preceding gene: 157370879

Following gene: 157370882

Centisome position: 53.4

GC content: 58.44

Gene sequence:

>900_bases
GTGATTTTCAATCTGCAGCGTTATTCCACCCATGATGGCCCAGGCATTCGCAGCGTAGTGTTTCTAAAGGGGTGCTCGCT
TGGCTGCCTCTGGTGCCAGAACCCGGAAAGCCGGGCGCGAAAGGCAGACCTGTTGTTCGACCCACGCCTGTGTCTTGGCG
GCTGCACGCTGTGTATCGAAGACAATACGCAGGCTGTCCGGCGGATAGACGATAGCCTGGTGATCCAGCGCGATTTACTC
AGCCATGAGGATTATGCCGCATTGGCTGCCCGTTGCCCAACCGGCGCGCTCAGCCTGTGCGGCAGCGCGATAAATATCGA
CGATATCATGGCCGAAGTTCTGCGCGACAGACCCTTTTATTTGCGCACCGGCGGTGGGCTGACGCTGTCCGGCGGTGAAC
CCTTTATGCAACCGACCGTGGCGGCTGAACTGCTGCGCCGCAGTCGTGAGGCCGGTATTCATACCGCGGTGGAGTCTTGT
CTGCATACCCCCTGGTCATACATCGCCCCGTCACTGCCCTGGCTTGACCTGATGCTGGCGGATCTGAAACACGTCGATGA
AAGGCGCTTCAAACAGTGGACCGGCGGCTCCGCCAAACGAGTGATGGACAATTTTCGTCGGCTGGCCGAGCGCGGTACCC
AAACTATTGTGCGCGTACCACTGATCCCGGAGTTTAACGCCGATCGCCACTCTGTCCGTGCGATTGTCGATTTCGCCGCC
GATGAAGCCGGCGCCAAAGAGATTCATTTTTTGCCATACCACACACTGGGCATCAACAAATACAACCTGCTCGGCGAACC
CTATCACGCTGCCCGTACCCCACTGGATGCGCCCGAACTGCTGGCCTATGCCGAGCAGTACGCCAACACCAAAGGGCTGA
CCGCTATTTTGCGAGGATAA

Upstream 100 bases:

>100_bases
GTAGGACATGCAATAGGTTTATATTTGATCGAAATCAAATCAACTTTCATTCGAAACAACTTTAATGAAAGCAGCAAAGC
AACCGAATGACAGGTGACTC

Downstream 100 bases:

>100_bases
CATCATGACCACGCTGGACCTGACCACCCTGACCGCACGCACCCTGGCCCACAAGAACGCGTTGATCCATATCGTCAAAC
CGCCGGTTTGCACCGAACGC

Product: glycyl-radical activating family protein

Products: NA

Alternate protein names: Formate-C-acetyltransferase-activating enzyme 3; PFL-activating enzyme 3 [H]

Number of amino acids: Translated: 299; Mature: 299

Protein sequence:

>299_residues
MIFNLQRYSTHDGPGIRSVVFLKGCSLGCLWCQNPESRARKADLLFDPRLCLGGCTLCIEDNTQAVRRIDDSLVIQRDLL
SHEDYAALAARCPTGALSLCGSAINIDDIMAEVLRDRPFYLRTGGGLTLSGGEPFMQPTVAAELLRRSREAGIHTAVESC
LHTPWSYIAPSLPWLDLMLADLKHVDERRFKQWTGGSAKRVMDNFRRLAERGTQTIVRVPLIPEFNADRHSVRAIVDFAA
DEAGAKEIHFLPYHTLGINKYNLLGEPYHAARTPLDAPELLAYAEQYANTKGLTAILRG

Sequences:

>Translated_299_residues
MIFNLQRYSTHDGPGIRSVVFLKGCSLGCLWCQNPESRARKADLLFDPRLCLGGCTLCIEDNTQAVRRIDDSLVIQRDLL
SHEDYAALAARCPTGALSLCGSAINIDDIMAEVLRDRPFYLRTGGGLTLSGGEPFMQPTVAAELLRRSREAGIHTAVESC
LHTPWSYIAPSLPWLDLMLADLKHVDERRFKQWTGGSAKRVMDNFRRLAERGTQTIVRVPLIPEFNADRHSVRAIVDFAA
DEAGAKEIHFLPYHTLGINKYNLLGEPYHAARTPLDAPELLAYAEQYANTKGLTAILRG
>Mature_299_residues
MIFNLQRYSTHDGPGIRSVVFLKGCSLGCLWCQNPESRARKADLLFDPRLCLGGCTLCIEDNTQAVRRIDDSLVIQRDLL
SHEDYAALAARCPTGALSLCGSAINIDDIMAEVLRDRPFYLRTGGGLTLSGGEPFMQPTVAAELLRRSREAGIHTAVESC
LHTPWSYIAPSLPWLDLMLADLKHVDERRFKQWTGGSAKRVMDNFRRLAERGTQTIVRVPLIPEFNADRHSVRAIVDFAA
DEAGAKEIHFLPYHTLGINKYNLLGEPYHAARTPLDAPELLAYAEQYANTKGLTAILRG

Specific function: Activation of pyruvate formate-lyase 2 under anaerobic conditions by generation of an organic free radical, using S- adenosylmethionine and reduced flavodoxin as cosubstrates to produce 5'-deoxy-adenosine [H]

COG id: COG1180

COG function: function code O; Pyruvate-formate lyase-activating enzyme

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the organic radical-activating enzymes family [H]

Homologues:

Organism=Escherichia coli, GI226510931, Length=299, Percent_Identity=65.8862876254181, Blast_Score=392, Evalue=1e-110,
Organism=Escherichia coli, GI1790389, Length=298, Percent_Identity=34.2281879194631, Blast_Score=168, Evalue=5e-43,
Organism=Escherichia coli, GI1787130, Length=172, Percent_Identity=29.6511627906977, Blast_Score=92, Evalue=5e-20,
Organism=Escherichia coli, GI1790839, Length=290, Percent_Identity=25.1724137931034, Blast_Score=65, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012839
- InterPro:   IPR011352
- InterPro:   IPR001989
- InterPro:   IPR007197 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: =1.97.1.4 [H]

Molecular weight: Translated: 33248; Mature: 33248

Theoretical pI: Translated: 7.21; Mature: 7.21

Prosite motif: PS01087 RADICAL_ACTIVATING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
3.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIFNLQRYSTHDGPGIRSVVFLKGCSLGCLWCQNPESRARKADLLFDPRLCLGGCTLCIE
CEEECCCCCCCCCCCCEEEEEEECCCEEEEECCCCHHHHHHCCCEECCCHHHCCEEEEEE
DNTQAVRRIDDSLVIQRDLLSHEDYAALAARCPTGALSLCGSAINIDDIMAEVLRDRPFY
CCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHCCCCCCHHHHHHHHHHCCCCE
LRTGGGLTLSGGEPFMQPTVAAELLRRSREAGIHTAVESCLHTPWSYIAPSLPWLDLMLA
EEECCCEEECCCCCCCCHHHHHHHHHHHHHCHHHHHHHHHHCCCHHHHCCCCCHHHHHHH
DLKHVDERRFKQWTGGSAKRVMDNFRRLAERGTQTIVRVPLIPEFNADRHSVRAIVDFAA
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCHHHHHHHHHHHH
DEAGAKEIHFLPYHTLGINKYNLLGEPYHAARTPLDAPELLAYAEQYANTKGLTAILRG
CCCCCCEEEEECEEEECCCCHHCCCCCHHHHCCCCCHHHHHHHHHHHCCCCCCEEEECC
>Mature Secondary Structure
MIFNLQRYSTHDGPGIRSVVFLKGCSLGCLWCQNPESRARKADLLFDPRLCLGGCTLCIE
CEEECCCCCCCCCCCCEEEEEEECCCEEEEECCCCHHHHHHCCCEECCCHHHCCEEEEEE
DNTQAVRRIDDSLVIQRDLLSHEDYAALAARCPTGALSLCGSAINIDDIMAEVLRDRPFY
CCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHCCCCCCHHHHHHHHHHCCCCE
LRTGGGLTLSGGEPFMQPTVAAELLRRSREAGIHTAVESCLHTPWSYIAPSLPWLDLMLA
EEECCCEEECCCCCCCCHHHHHHHHHHHHHCHHHHHHHHHHCCCHHHHCCCCCHHHHHHH
DLKHVDERRFKQWTGGSAKRVMDNFRRLAERGTQTIVRVPLIPEFNADRHSVRAIVDFAA
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCHHHHHHHHHHHH
DEAGAKEIHFLPYHTLGINKYNLLGEPYHAARTPLDAPELLAYAEQYANTKGLTAILRG
CCCCCCEEEEECEEEECCCCHHCCCCCHHHHCCCCCHHHHHHHHHHHCCCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503 [H]