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The map label for this gene is hisA

Identifier: 157369854

GI number: 157369854

Start: 1765713

End: 1766450

Strand: Reverse

Name: hisA

Synonym: Spro_1611

Alternate gene names: 157369854

Gene position: 1766450-1765713 (Counterclockwise)

Preceding gene: 157369855

Following gene: 157369853

Centisome position: 32.42

GC content: 60.3

Gene sequence:

>738_bases
ATGATTATTCCGGCTTTGGATTTGATCGACGGCAACGTAGTGCGTCTGCATCAGGGCGATTACGGCCAGCAGCGCGACTA
CGGTAACGATCCATTGTTACGCCTGCAGGACTATCAGCAACAGGGCGCACAGGTGCTGCATCTGGTCGACCTGACCGGCG
CGAAAGACCCGACCGCGCGCCAAATCCCCCTGCTGCGCAAGCTGCTGGCGGGGGTTAACGTACCGGTACAGGTTGGCGGC
GGCATTCGCAATGAGCAGGACGTCGCTGCCCTGCTGGAGGCCGGCGCAACCCGCGTGGTAATCGGCTCCACCGCGGTAAA
ACAGCCGCAGCTGGTACAGGGCTGGTTCGAACGCTACGGCGCGGACGCCATGGTACTGGCGCTGGACGTGCGCATTGACG
CAGACGGCACCAAACGCGTGGCGATCAGCGGCTGGCAGGAAAACTCGGATGCCACGCTGGAACAGGTGGTTGAGCAGTTC
TTGCCTTATGGCCTCAAACACGTGCTGTGCACCGATATTTCCCGTGACGGGACATTGGCCGGTTCCAACGTGGCGCTGTA
TCAGGAAATCAGCCAACGATATCCGCAGATCGCCTTCCAGGCCTCTGGCGGTATCGGTAATCTGGATGATATCGCCCAGC
TGCGCGGCAGTGGCGTTGAGGGTGTGATTGTGGGGCGTGCCCTGCTGGAAGGTAAGTTTAGCGTTGAGGAGGCAATCGCA
TGCTGGCAAAACGGATAA

Upstream 100 bases:

>100_bases
CCGTGCAAAAAGACAATTTCTTCGGCGTGCAGTTTCATCCAGAGCGATCTGGCGCGGCCGGTGCGCAACTGTTGAAAAAC
TTTCTGGAGATGTAGGCAGC

Downstream 100 bases:

>100_bases
TCCCGTGCCTGGATGTTAAAGACGGGCAGGTAGTGAAAGGCGTGCAGTTCCGCAACCATGAAATTATCGGCGACATCGTG
CCACTGGCGCAGCGCTATGC

Product: 1-(5-phosphoribosyl)-5-[(5- phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase

Products: NA

Alternate protein names: Phosphoribosylformimino-5-aminoimidazole carboxamide ribotide isomerase

Number of amino acids: Translated: 245; Mature: 245

Protein sequence:

>245_residues
MIIPALDLIDGNVVRLHQGDYGQQRDYGNDPLLRLQDYQQQGAQVLHLVDLTGAKDPTARQIPLLRKLLAGVNVPVQVGG
GIRNEQDVAALLEAGATRVVIGSTAVKQPQLVQGWFERYGADAMVLALDVRIDADGTKRVAISGWQENSDATLEQVVEQF
LPYGLKHVLCTDISRDGTLAGSNVALYQEISQRYPQIAFQASGGIGNLDDIAQLRGSGVEGVIVGRALLEGKFSVEEAIA
CWQNG

Sequences:

>Translated_245_residues
MIIPALDLIDGNVVRLHQGDYGQQRDYGNDPLLRLQDYQQQGAQVLHLVDLTGAKDPTARQIPLLRKLLAGVNVPVQVGG
GIRNEQDVAALLEAGATRVVIGSTAVKQPQLVQGWFERYGADAMVLALDVRIDADGTKRVAISGWQENSDATLEQVVEQF
LPYGLKHVLCTDISRDGTLAGSNVALYQEISQRYPQIAFQASGGIGNLDDIAQLRGSGVEGVIVGRALLEGKFSVEEAIA
CWQNG
>Mature_245_residues
MIIPALDLIDGNVVRLHQGDYGQQRDYGNDPLLRLQDYQQQGAQVLHLVDLTGAKDPTARQIPLLRKLLAGVNVPVQVGG
GIRNEQDVAALLEAGATRVVIGSTAVKQPQLVQGWFERYGADAMVLALDVRIDADGTKRVAISGWQENSDATLEQVVEQF
LPYGLKHVLCTDISRDGTLAGSNVALYQEISQRYPQIAFQASGGIGNLDDIAQLRGSGVEGVIVGRALLEGKFSVEEAIA
CWQNG

Specific function: Histidine biosynthesis; fourth step. [C]

COG id: COG0106

COG function: function code E; Phosphoribosylformimino-5-aminoimidazole carboxamide ribonucleotide (ProFAR) isomerase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hisA/hisF family

Homologues:

Organism=Escherichia coli, GI87082028, Length=244, Percent_Identity=78.6885245901639, Blast_Score=405, Evalue=1e-114,
Organism=Escherichia coli, GI1788336, Length=234, Percent_Identity=24.3589743589744, Blast_Score=72, Evalue=4e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HIS4_SERP5 (A8GC75)

Other databases:

- EMBL:   CP000826
- RefSeq:   YP_001477843.1
- ProteinModelPortal:   A8GC75
- SMR:   A8GC75
- STRING:   A8GC75
- GeneID:   5603804
- GenomeReviews:   CP000826_GR
- KEGG:   spe:Spro_1611
- eggNOG:   COG0106
- HOGENOM:   HBG541613
- OMA:   SIIYTDI
- ProtClustDB:   PRK00748
- BioCyc:   SPRO399741:SPRO_1611-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01014
- InterPro:   IPR013785
- InterPro:   IPR006062
- InterPro:   IPR006063
- InterPro:   IPR023016
- InterPro:   IPR011060
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00007

Pfam domain/function: PF00977 His_biosynth; SSF51366 RibP_bind_barrel

EC number: =5.3.1.16

Molecular weight: Translated: 26413; Mature: 26413

Theoretical pI: Translated: 4.57; Mature: 4.57

Prosite motif: NA

Important sites: ACT_SITE 7-7 ACT_SITE 129-129

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIIPALDLIDGNVVRLHQGDYGQQRDYGNDPLLRLQDYQQQGAQVLHLVDLTGAKDPTAR
CCCCCEEECCCCEEEEECCCCCCCCCCCCCCCEEHHHHHHCCHHEEEEEECCCCCCCCHH
QIPLLRKLLAGVNVPVQVGGGIRNEQDVAALLEAGATRVVIGSTAVKQPQLVQGWFERYG
HHHHHHHHHHCCCCCEEECCCCCCHHHHHHHHHCCCCEEEECCCCCCCCHHHHHHHHHHC
ADAMVLALDVRIDADGTKRVAISGWQENSDATLEQVVEQFLPYGLKHVLCTDISRDGTLA
CCEEEEEEEEEECCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
GSNVALYQEISQRYPQIAFQASGGIGNLDDIAQLRGSGVEGVIVGRALLEGKFSVEEAIA
CCCHHHHHHHHHHCCEEEEEECCCCCCHHHHHHHHCCCCCEEEEHHHHHCCCCCHHHHHH
CWQNG
HHCCC
>Mature Secondary Structure
MIIPALDLIDGNVVRLHQGDYGQQRDYGNDPLLRLQDYQQQGAQVLHLVDLTGAKDPTAR
CCCCCEEECCCCEEEEECCCCCCCCCCCCCCCEEHHHHHHCCHHEEEEEECCCCCCCCHH
QIPLLRKLLAGVNVPVQVGGGIRNEQDVAALLEAGATRVVIGSTAVKQPQLVQGWFERYG
HHHHHHHHHHCCCCCEEECCCCCCHHHHHHHHHCCCCEEEECCCCCCCCHHHHHHHHHHC
ADAMVLALDVRIDADGTKRVAISGWQENSDATLEQVVEQFLPYGLKHVLCTDISRDGTLA
CCEEEEEEEEEECCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
GSNVALYQEISQRYPQIAFQASGGIGNLDDIAQLRGSGVEGVIVGRALLEGKFSVEEAIA
CCCHHHHHHHHHHCCEEEEEECCCCCCHHHHHHHHCCCCCEEEEHHHHHCCCCCHHHHHH
CWQNG
HHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA