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The map label for this gene is hisH [H]

Identifier: 157369855

GI number: 157369855

Start: 1766456

End: 1767046

Strand: Reverse

Name: hisH [H]

Synonym: Spro_1612

Alternate gene names: 157369855

Gene position: 1767046-1766456 (Counterclockwise)

Preceding gene: 157369856

Following gene: 157369854

Centisome position: 32.43

GC content: 60.07

Gene sequence:

>591_bases
ATGGACGTGGTGATTCTGGACACCGGCTGCGCTAACCTGGCCTCCGTCACCTACGCGGTGCAGCGGTTGGGCTACCGCCC
GGAAGTGAGCCGTGACCCGGAGATCGTACTGCGCGCCGACAAGCTTTTCCTGCCAGGCGTGGGTACCGCGCAGGCGGCGA
TGGATCAACTGGAACAGCGCGAACTGATTGAGTTGATCAAGGCCTGTACCCAACCGGTGCTGGGGATTTGCCTCGGCATG
CAGTTGCTGGCAAAAAGCAGCGAAGAGAACGGCGGCATCGACACGCTGGGCATTATCGATACTCCGGTAACCCAGATGAC
CGACTTCGGCCTGCCGCTGCCGCACATGGGCTGGAATCAGGTGTCTGCGCAGGCGGGTCATCACCTGTTCCGCGGCATCG
AAGACGGCGCCTACTTCTACTTCGTTCACAGCTATGCGATGCCGATATGCCCAAGCACCATCGCCCAGGCGAATTACGGC
GAGCCCTTCACCGCCGCCGTGCAAAAAGACAATTTCTTCGGCGTGCAGTTTCATCCAGAGCGATCTGGCGCGGCCGGTGC
GCAACTGTTGAAAAACTTTCTGGAGATGTAG

Upstream 100 bases:

>100_bases
ACCACCGTGTCGAAAGCCTGTTCAAAGTGTTTGGCCGCACGCTGCGGCAGGCGATCCGCGTTGAGGGCAACACCCTCCCG
AGCTCGAAAGGAGTGCTGTG

Downstream 100 bases:

>100_bases
GCAGCATGATTATTCCGGCTTTGGATTTGATCGACGGCAACGTAGTGCGTCTGCATCAGGGCGATTACGGCCAGCAGCGC
GACTACGGTAACGATCCATT

Product: imidazole glycerol phosphate synthase subunit HisH

Products: NA

Alternate protein names: IGP synthase glutamine amidotransferase subunit; IGP synthase subunit hisH; ImGP synthase subunit hisH; IGPS subunit hisH [H]

Number of amino acids: Translated: 196; Mature: 196

Protein sequence:

>196_residues
MDVVILDTGCANLASVTYAVQRLGYRPEVSRDPEIVLRADKLFLPGVGTAQAAMDQLEQRELIELIKACTQPVLGICLGM
QLLAKSSEENGGIDTLGIIDTPVTQMTDFGLPLPHMGWNQVSAQAGHHLFRGIEDGAYFYFVHSYAMPICPSTIAQANYG
EPFTAAVQKDNFFGVQFHPERSGAAGAQLLKNFLEM

Sequences:

>Translated_196_residues
MDVVILDTGCANLASVTYAVQRLGYRPEVSRDPEIVLRADKLFLPGVGTAQAAMDQLEQRELIELIKACTQPVLGICLGM
QLLAKSSEENGGIDTLGIIDTPVTQMTDFGLPLPHMGWNQVSAQAGHHLFRGIEDGAYFYFVHSYAMPICPSTIAQANYG
EPFTAAVQKDNFFGVQFHPERSGAAGAQLLKNFLEM
>Mature_196_residues
MDVVILDTGCANLASVTYAVQRLGYRPEVSRDPEIVLRADKLFLPGVGTAQAAMDQLEQRELIELIKACTQPVLGICLGM
QLLAKSSEENGGIDTLGIIDTPVTQMTDFGLPLPHMGWNQVSAQAGHHLFRGIEDGAYFYFVHSYAMPICPSTIAQANYG
EPFTAAVQKDNFFGVQFHPERSGAAGAQLLKNFLEM

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisH subunit provides the glutamine amidotransferase activity that produces the ammonia necessary to hisF for the synthesis of IGP and AICAR [H]

COG id: COG0118

COG function: function code E; Glutamine amidotransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1788334, Length=196, Percent_Identity=79.5918367346939, Blast_Score=330, Evalue=4e-92,
Organism=Saccharomyces cerevisiae, GI6319725, Length=209, Percent_Identity=35.4066985645933, Blast_Score=121, Evalue=6e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011702
- InterPro:   IPR017926
- InterPro:   IPR000991
- InterPro:   IPR010139
- InterPro:   IPR016226 [H]

Pfam domain/function: PF00117 GATase [H]

EC number: 2.4.2.-

Molecular weight: Translated: 21349; Mature: 21349

Theoretical pI: Translated: 4.64; Mature: 4.64

Prosite motif: PS00442 GATASE_TYPE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDVVILDTGCANLASVTYAVQRLGYRPEVSRDPEIVLRADKLFLPGVGTAQAAMDQLEQR
CCEEEEECCCHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCEECCCCCHHHHHHHHHHHH
ELIELIKACTQPVLGICLGMQLLAKSSEENGGIDTLGIIDTPVTQMTDFGLPLPHMGWNQ
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCHHHHHHCCCCCCCCCCHH
VSAQAGHHLFRGIEDGAYFYFVHSYAMPICPSTIAQANYGEPFTAAVQKDNFFGVQFHPE
HHHHHHHHHHHCCCCCEEEEEEEHHHCCCCCHHHHCCCCCCCCEEEEECCCEEEEEECCC
RSGAAGAQLLKNFLEM
CCCCHHHHHHHHHHCC
>Mature Secondary Structure
MDVVILDTGCANLASVTYAVQRLGYRPEVSRDPEIVLRADKLFLPGVGTAQAAMDQLEQR
CCEEEEECCCHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCEECCCCCHHHHHHHHHHHH
ELIELIKACTQPVLGICLGMQLLAKSSEENGGIDTLGIIDTPVTQMTDFGLPLPHMGWNQ
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCHHHHHHCCCCCCCCCCHH
VSAQAGHHLFRGIEDGAYFYFVHSYAMPICPSTIAQANYGEPFTAAVQKDNFFGVQFHPE
HHHHHHHHHHHCCCCCEEEEEEEHHHCCCCCHHHHCCCCCCCCEEEEECCCEEEEEECCC
RSGAAGAQLLKNFLEM
CCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Glycosyltransferases; Pentosyltransferases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11586360; 12142430 [H]