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The map label for this gene is hisF [H]

Identifier: 157369853

GI number: 157369853

Start: 1764955

End: 1765731

Strand: Reverse

Name: hisF [H]

Synonym: Spro_1610

Alternate gene names: 157369853

Gene position: 1765731-1764955 (Counterclockwise)

Preceding gene: 157369854

Following gene: 157369852

Centisome position: 32.41

GC content: 55.73

Gene sequence:

>777_bases
ATGCTGGCAAAACGGATAATCCCGTGCCTGGATGTTAAAGACGGGCAGGTAGTGAAAGGCGTGCAGTTCCGCAACCATGA
AATTATCGGCGACATCGTGCCACTGGCGCAGCGCTATGCGCAGGAAGGCGCAGACGAACTGGTGTTTTACGATATCACCG
CGTCAAGCGATGGCCGGGTGGTAGATAAAAGCTGGGTATCACGCGTGGCAGAGGTGATTGATATCCCCTTCTGCGTCGCC
GGCGGGATTAAAAGCCCGGAAGACGCCAGCCAGATCCTCTCTTTCGGCGCCGACAAGATTTCCATCAACTCCCCGGCGCT
GGCCGACCCGACGCTGATTAGCCGCCTGGCGGATCGCTTTGGCGTACAGTGCATTGTGGTGGGTATTGATACCTGGTTCG
AAGCCGAAACCGGCAAGTATCACGTTAACCAATATACCGGAGACGAAAGCCGCACCCGCGTTACTGAGTGGGAAACCCTG
GATTGGGTGAAAGAAGTGCAGAAACGCGGCGCCGGCGAAATTGTGCTGAATATGATGAATCAGGACGGCGTGCGTAACGG
CTACGATTTGGAACAGCTACGCCTGGTGCGTGAGGTGTGCAAAGTGCCATTGATCGCCTCCGGCGGCGCAGGCACCATGG
AACACTTCCTGGAAGCCTTCCGCGATGTCGATGTCGATGGCGCACTGGCCGCTTCGGTGTTCCACAAACAAATCATTAAT
ATCGGTGACCTGAAAAGGTTCTTGTCCGAACAAGGTGTGGAGATCCGCCTGTGTTAA

Upstream 100 bases:

>100_bases
ATCTGGATGATATCGCCCAGCTGCGCGGCAGTGGCGTTGAGGGTGTGATTGTGGGGCGTGCCCTGCTGGAAGGTAAGTTT
AGCGTTGAGGAGGCAATCGC

Downstream 100 bases:

>100_bases
CAGAACAACAAAGAAACCTGCTGGACTGGGAAAAAACTGACCACCTGCTACCGGTAATCGTCCAGCATGCCGTATCCGGC
GAAGTGCTGATGCTCGGCTA

Product: imidazole glycerol phosphate synthase subunit HisF

Products: NA

Alternate protein names: IGP synthase cyclase subunit; IGP synthase subunit hisF; ImGP synthase subunit hisF; IGPS subunit hisF [H]

Number of amino acids: Translated: 258; Mature: 258

Protein sequence:

>258_residues
MLAKRIIPCLDVKDGQVVKGVQFRNHEIIGDIVPLAQRYAQEGADELVFYDITASSDGRVVDKSWVSRVAEVIDIPFCVA
GGIKSPEDASQILSFGADKISINSPALADPTLISRLADRFGVQCIVVGIDTWFEAETGKYHVNQYTGDESRTRVTEWETL
DWVKEVQKRGAGEIVLNMMNQDGVRNGYDLEQLRLVREVCKVPLIASGGAGTMEHFLEAFRDVDVDGALAASVFHKQIIN
IGDLKRFLSEQGVEIRLC

Sequences:

>Translated_258_residues
MLAKRIIPCLDVKDGQVVKGVQFRNHEIIGDIVPLAQRYAQEGADELVFYDITASSDGRVVDKSWVSRVAEVIDIPFCVA
GGIKSPEDASQILSFGADKISINSPALADPTLISRLADRFGVQCIVVGIDTWFEAETGKYHVNQYTGDESRTRVTEWETL
DWVKEVQKRGAGEIVLNMMNQDGVRNGYDLEQLRLVREVCKVPLIASGGAGTMEHFLEAFRDVDVDGALAASVFHKQIIN
IGDLKRFLSEQGVEIRLC
>Mature_258_residues
MLAKRIIPCLDVKDGQVVKGVQFRNHEIIGDIVPLAQRYAQEGADELVFYDITASSDGRVVDKSWVSRVAEVIDIPFCVA
GGIKSPEDASQILSFGADKISINSPALADPTLISRLADRFGVQCIVVGIDTWFEAETGKYHVNQYTGDESRTRVTEWETL
DWVKEVQKRGAGEIVLNMMNQDGVRNGYDLEQLRLVREVCKVPLIASGGAGTMEHFLEAFRDVDVDGALAASVFHKQIIN
IGDLKRFLSEQGVEIRLC

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisF subunit catalyzes the cyclization activity that produces IGP and AICAR from PRFAR using the ammonia provided by the hisH subunit [H]

COG id: COG0107

COG function: function code E; Imidazoleglycerol-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hisA/hisF family [H]

Homologues:

Organism=Escherichia coli, GI1788336, Length=258, Percent_Identity=91.8604651162791, Blast_Score=494, Evalue=1e-141,
Organism=Escherichia coli, GI87082028, Length=231, Percent_Identity=25.974025974026, Blast_Score=68, Evalue=6e-13,
Organism=Saccharomyces cerevisiae, GI6319725, Length=318, Percent_Identity=32.3899371069182, Blast_Score=136, Evalue=3e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006062
- InterPro:   IPR004651
- InterPro:   IPR011060 [H]

Pfam domain/function: PF00977 His_biosynth [H]

EC number: 4.1.3.-

Molecular weight: Translated: 28542; Mature: 28542

Theoretical pI: Translated: 4.66; Mature: 4.66

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLAKRIIPCLDVKDGQVVKGVQFRNHEIIGDIVPLAQRYAQEGADELVFYDITASSDGRV
CCHHHCCCCCCCCCCCEEECEEECCCHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCE
VDKSWVSRVAEVIDIPFCVAGGIKSPEDASQILSFGADKISINSPALADPTLISRLADRF
ECHHHHHHHHHHHCCCHHHCCCCCCCHHHHHHHHCCCCEEECCCCCCCCHHHHHHHHHHH
GVQCIVVGIDTWFEAETGKYHVNQYTGDESRTRVTEWETLDWVKEVQKRGAGEIVLNMMN
CCEEEEEEECCCEECCCCCEEEEEECCCCCCCEEHHHHHHHHHHHHHHCCCCCEEHHHHH
QDGVRNGYDLEQLRLVREVCKVPLIASGGAGTMEHFLEAFRDVDVDGALAASVFHKQIIN
CCCCCCCCCHHHHHHHHHHHCCCEEECCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHC
IGDLKRFLSEQGVEIRLC
HHHHHHHHHHCCCEEEEC
>Mature Secondary Structure
MLAKRIIPCLDVKDGQVVKGVQFRNHEIIGDIVPLAQRYAQEGADELVFYDITASSDGRV
CCHHHCCCCCCCCCCCEEECEEECCCHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCE
VDKSWVSRVAEVIDIPFCVAGGIKSPEDASQILSFGADKISINSPALADPTLISRLADRF
ECHHHHHHHHHHHCCCHHHCCCCCCCHHHHHHHHCCCCEEECCCCCCCCHHHHHHHHHHH
GVQCIVVGIDTWFEAETGKYHVNQYTGDESRTRVTEWETLDWVKEVQKRGAGEIVLNMMN
CCEEEEEEECCCEECCCCCEEEEEECCCCCCCEEHHHHHHHHHHHHHHCCCCCEEHHHHH
QDGVRNGYDLEQLRLVREVCKVPLIASGGAGTMEHFLEAFRDVDVDGALAASVFHKQIIN
CCCCCCCCCHHHHHHHHHHHCCCEEECCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHC
IGDLKRFLSEQGVEIRLC
HHHHHHHHHHCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Lyases; Carbon-Nitrogen Lyases; Amidine-Lyases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA