Click here to switch to the map view.

The map label for this gene is frmA [H]

Identifier: 157369800

GI number: 157369800

Start: 1696247

End: 1697374

Strand: Reverse

Name: frmA [H]

Synonym: Spro_1557

Alternate gene names: 157369800

Gene position: 1697374-1696247 (Counterclockwise)

Preceding gene: 157369801

Following gene: 157369799

Centisome position: 31.15

GC content: 57.62

Gene sequence:

>1128_bases
ATGGAAATGATCAAAACCCGTGCCGCCGTTGCCTGGGGCCCGAACCAGCCGCTGAAAATCGAAGAAGTGGATCTGATGCC
GCCGCAAAAAGGCGAAGTGCTGGTGCGGATCGTCGCCACCGGCGTCTGCCATACCGACGCCTACACCCTGTCCGGTGCCG
ATCCTGAAGGCGTATTCCCGGCGATCCTCGGCCATGAAGGCGGCGGCGTGGTAGAAGCCATCGGTGAAGGCGTAACCAGC
GTAGCGGTTGGCGATCACGTGATCCCGTTGTACACGCCGGAATGCGGCGAGTGCAAATTCTGTAAATCCGGCAAAACCAA
CCTGTGTCAGGCGATCCGTGCTACCCAGGGCAAAGGCCTGATGCCGGATGGCACCACCCGTTTCTTCAAAGACGGCAAGC
CTATCTTCCACTACATGGGCACTTCAACCTTCTCCGAATACACCGTAGTGCCGGAAATCTCACTGGCAAAAATCAGTAAA
GAAGCCCCGCTGGAAGAAGTCTGCCTGCTGGGCTGCGGCGTTACCACCGGCATGGGCGCGGTAATCAACACCGCCAAAGT
GCAAAAAGGCGATACCGTGGCCATCTTCGGCCTCGGCGGCATTGGCCTGTCGGCAATCATCGGCGCACAGATGGCGGGTG
CAGGCCGCATCATCGGCATTGATATCAACACCAGCAAGTTCGACCTCGCGCGCAAGCTGGGCGCCACCGATCTGATCAAC
CCGAAAGACTACGATAAACCTATTCAGGACGTGATCGTCGAACTGACCGACGGCGGCGTTGATTTCTCCTTCGAGTGTAT
CGGCAACGTCAACGTGATGCGCTCGGCGCTGGAATGCTGCCACAAAGGCTGGGGCGAATCGGTGATTATCGGCGTTGCCG
GTGCCGGTCAGGAAATTTCTACCCGTCCGTTCCAACTGGTGACCGGCCGCGTATGGCGCGGTTCCGCCTTTGGTGGCGTG
AAGGGCCGTTCGCAACTGCCGGGCATCGTCCAGCGTTATATGGACGGCGAGTTCGCGCTAAATGACTTTATTACCCACAC
CATGGGGCTGGAGCAGATTAACGAAGCGTTCGATTTGATGCACGAAGGCAAATCCATCCGTTCCGTTATTCACTTCGACA
AAAAATAA

Upstream 100 bases:

>100_bases
TTATTGAACAGGCAGAAACCCTCTCGGTTGAAAATACTGCCTGCGATTTACCCAATGCTATCGACATAGCCTGATTAAAA
ATAGACCACTGGAGACTATG

Downstream 100 bases:

>100_bases
GCCAGGAGGCAGAGATGACCTTGTCATTAGAACTTCTCGAAGAGCACCGTATGTTCGGCGGCTGGCAACAGCGTTATCGC
CATGCGGCGCAGAGCCTGAA

Product: S-(hydroxymethyl)glutathione dehydrogenase

Products: NA

Alternate protein names: Alcohol dehydrogenase class-3; Alcohol dehydrogenase class-III; Glutathione-dependent formaldehyde dehydrogenase; FALDH; FDH; GSH-FDH [H]

Number of amino acids: Translated: 375; Mature: 375

Protein sequence:

>375_residues
MEMIKTRAAVAWGPNQPLKIEEVDLMPPQKGEVLVRIVATGVCHTDAYTLSGADPEGVFPAILGHEGGGVVEAIGEGVTS
VAVGDHVIPLYTPECGECKFCKSGKTNLCQAIRATQGKGLMPDGTTRFFKDGKPIFHYMGTSTFSEYTVVPEISLAKISK
EAPLEEVCLLGCGVTTGMGAVINTAKVQKGDTVAIFGLGGIGLSAIIGAQMAGAGRIIGIDINTSKFDLARKLGATDLIN
PKDYDKPIQDVIVELTDGGVDFSFECIGNVNVMRSALECCHKGWGESVIIGVAGAGQEISTRPFQLVTGRVWRGSAFGGV
KGRSQLPGIVQRYMDGEFALNDFITHTMGLEQINEAFDLMHEGKSIRSVIHFDKK

Sequences:

>Translated_375_residues
MEMIKTRAAVAWGPNQPLKIEEVDLMPPQKGEVLVRIVATGVCHTDAYTLSGADPEGVFPAILGHEGGGVVEAIGEGVTS
VAVGDHVIPLYTPECGECKFCKSGKTNLCQAIRATQGKGLMPDGTTRFFKDGKPIFHYMGTSTFSEYTVVPEISLAKISK
EAPLEEVCLLGCGVTTGMGAVINTAKVQKGDTVAIFGLGGIGLSAIIGAQMAGAGRIIGIDINTSKFDLARKLGATDLIN
PKDYDKPIQDVIVELTDGGVDFSFECIGNVNVMRSALECCHKGWGESVIIGVAGAGQEISTRPFQLVTGRVWRGSAFGGV
KGRSQLPGIVQRYMDGEFALNDFITHTMGLEQINEAFDLMHEGKSIRSVIHFDKK
>Mature_375_residues
MEMIKTRAAVAWGPNQPLKIEEVDLMPPQKGEVLVRIVATGVCHTDAYTLSGADPEGVFPAILGHEGGGVVEAIGEGVTS
VAVGDHVIPLYTPECGECKFCKSGKTNLCQAIRATQGKGLMPDGTTRFFKDGKPIFHYMGTSTFSEYTVVPEISLAKISK
EAPLEEVCLLGCGVTTGMGAVINTAKVQKGDTVAIFGLGGIGLSAIIGAQMAGAGRIIGIDINTSKFDLARKLGATDLIN
PKDYDKPIQDVIVELTDGGVDFSFECIGNVNVMRSALECCHKGWGESVIIGVAGAGQEISTRPFQLVTGRVWRGSAFGGV
KGRSQLPGIVQRYMDGEFALNDFITHTMGLEQINEAFDLMHEGKSIRSVIHFDKK

Specific function: Has High Formaldehyde Dehydrogenase Activity In The Presence Of Glutathione And Catalyzes The Oxidation Of Normal Alcohols In A Reaction That Is Not Gsh-Dependent. In Addition, Hemithiolacetals Other Than Those Formed From Gsh, Including Omega-Thiol Fatty

COG id: COG1062

COG function: function code C; Zn-dependent alcohol dehydrogenases, class III

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the zinc-containing alcohol dehydrogenase family. Class-III subfamily [H]

Homologues:

Organism=Homo sapiens, GI71565154, Length=369, Percent_Identity=63.9566395663957, Blast_Score=505, Evalue=1e-143,
Organism=Homo sapiens, GI34577061, Length=371, Percent_Identity=52.0215633423181, Blast_Score=416, Evalue=1e-116,
Organism=Homo sapiens, GI4501929, Length=373, Percent_Identity=50.9383378016086, Blast_Score=403, Evalue=1e-112,
Organism=Homo sapiens, GI4501933, Length=371, Percent_Identity=50.4043126684636, Blast_Score=401, Evalue=1e-112,
Organism=Homo sapiens, GI71565152, Length=375, Percent_Identity=51.4666666666667, Blast_Score=399, Evalue=1e-111,
Organism=Homo sapiens, GI156523966, Length=369, Percent_Identity=48.2384823848238, Blast_Score=378, Evalue=1e-105,
Organism=Homo sapiens, GI71743840, Length=372, Percent_Identity=48.3870967741936, Blast_Score=375, Evalue=1e-104,
Organism=Homo sapiens, GI262073058, Length=371, Percent_Identity=48.5175202156334, Blast_Score=375, Evalue=1e-104,
Organism=Homo sapiens, GI4501939, Length=363, Percent_Identity=48.4848484848485, Blast_Score=372, Evalue=1e-103,
Organism=Homo sapiens, GI156627571, Length=376, Percent_Identity=24.468085106383, Blast_Score=77, Evalue=3e-14,
Organism=Escherichia coli, GI1786552, Length=369, Percent_Identity=71.8157181571816, Blast_Score=542, Evalue=1e-155,
Organism=Escherichia coli, GI87082125, Length=380, Percent_Identity=26.0526315789474, Blast_Score=114, Evalue=9e-27,
Organism=Escherichia coli, GI1790045, Length=374, Percent_Identity=26.2032085561497, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1787863, Length=354, Percent_Identity=25.4237288135593, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1788073, Length=359, Percent_Identity=25.9052924791086, Blast_Score=86, Evalue=4e-18,
Organism=Escherichia coli, GI1786825, Length=274, Percent_Identity=28.4671532846715, Blast_Score=82, Evalue=7e-17,
Organism=Escherichia coli, GI87081918, Length=352, Percent_Identity=28.9772727272727, Blast_Score=79, Evalue=7e-16,
Organism=Escherichia coli, GI1788075, Length=371, Percent_Identity=24.2587601078167, Blast_Score=75, Evalue=8e-15,
Organism=Escherichia coli, GI226510992, Length=350, Percent_Identity=22.5714285714286, Blast_Score=66, Evalue=4e-12,
Organism=Escherichia coli, GI1786518, Length=372, Percent_Identity=23.9247311827957, Blast_Score=66, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI25146526, Length=377, Percent_Identity=58.6206896551724, Blast_Score=478, Evalue=1e-135,
Organism=Caenorhabditis elegans, GI71997431, Length=379, Percent_Identity=58.311345646438, Blast_Score=472, Evalue=1e-133,
Organism=Caenorhabditis elegans, GI17565904, Length=343, Percent_Identity=58.600583090379, Blast_Score=434, Evalue=1e-122,
Organism=Caenorhabditis elegans, GI17562876, Length=372, Percent_Identity=26.3440860215054, Blast_Score=100, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI17562584, Length=371, Percent_Identity=29.3800539083558, Blast_Score=99, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI17562878, Length=369, Percent_Identity=25.7452574525745, Blast_Score=89, Evalue=5e-18,
Organism=Caenorhabditis elegans, GI17562582, Length=256, Percent_Identity=29.296875, Blast_Score=80, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI71988145, Length=362, Percent_Identity=26.7955801104972, Blast_Score=80, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6320033, Length=375, Percent_Identity=58.6666666666667, Blast_Score=472, Evalue=1e-134,
Organism=Saccharomyces cerevisiae, GI6319621, Length=378, Percent_Identity=28.8359788359788, Blast_Score=94, Evalue=5e-20,
Organism=Saccharomyces cerevisiae, GI6323729, Length=379, Percent_Identity=27.9683377308707, Blast_Score=92, Evalue=9e-20,
Organism=Saccharomyces cerevisiae, GI6323099, Length=390, Percent_Identity=27.1794871794872, Blast_Score=92, Evalue=1e-19,
Organism=Saccharomyces cerevisiae, GI6323961, Length=371, Percent_Identity=26.9541778975741, Blast_Score=88, Evalue=3e-18,
Organism=Saccharomyces cerevisiae, GI6324486, Length=371, Percent_Identity=26.6846361185984, Blast_Score=87, Evalue=5e-18,
Organism=Saccharomyces cerevisiae, GI6319258, Length=269, Percent_Identity=26.0223048327138, Blast_Score=76, Evalue=9e-15,
Organism=Saccharomyces cerevisiae, GI6319257, Length=288, Percent_Identity=24.6527777777778, Blast_Score=67, Evalue=6e-12,
Organism=Drosophila melanogaster, GI17737895, Length=373, Percent_Identity=63.2707774798928, Blast_Score=509, Evalue=1e-144,
Organism=Drosophila melanogaster, GI17137530, Length=267, Percent_Identity=30.3370786516854, Blast_Score=90, Evalue=2e-18,
Organism=Drosophila melanogaster, GI17737897, Length=267, Percent_Identity=29.2134831460674, Blast_Score=80, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014183
- InterPro:   IPR013149
- InterPro:   IPR013154
- InterPro:   IPR002085
- InterPro:   IPR002328
- InterPro:   IPR011032
- InterPro:   IPR016040 [H]

Pfam domain/function: PF08240 ADH_N; PF00107 ADH_zinc_N [H]

EC number: =1.1.1.284; =1.1.1.1 [H]

Molecular weight: Translated: 39882; Mature: 39882

Theoretical pI: Translated: 5.65; Mature: 5.65

Prosite motif: PS00059 ADH_ZINC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEMIKTRAAVAWGPNQPLKIEEVDLMPPQKGEVLVRIVATGVCHTDAYTLSGADPEGVFP
CCCHHCCEEEEECCCCCCEEEEECCCCCCCCCEEEEEEECCCCCCCCEEECCCCCCCCCH
AILGHEGGGVVEAIGEGVTSVAVGDHVIPLYTPECGECKFCKSGKTNLCQAIRATQGKGL
HEECCCCCCHHHHHHCCHHEEEECCEEEEEECCCCCCCCCCCCCCHHHHHHHHHCCCCCC
MPDGTTRFFKDGKPIFHYMGTSTFSEYTVVPEISLAKISKEAPLEEVCLLGCGVTTGMGA
CCCCCHHHHHCCCCEEEECCCCCCCCEEECCCCHHHHHCCCCCHHHHHHHHCCHHHCCHH
VINTAKVQKGDTVAIFGLGGIGLSAIIGAQMAGAGRIIGIDINTSKFDLARKLGATDLIN
HEEHHEECCCCEEEEEEECCHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHCCCCCCCC
PKDYDKPIQDVIVELTDGGVDFSFECIGNVNVMRSALECCHKGWGESVIIGVAGAGQEIS
CCCCCCCHHHHHEEECCCCCCEEEEEECCHHHHHHHHHHHHCCCCCEEEEEEECCCCCCC
TRPFQLVTGRVWRGSAFGGVKGRSQLPGIVQRYMDGEFALNDFITHTMGLEQINEAFDLM
CCCEEEEECEEECCCCCCCCCCCCCCHHHHHHHCCCCHHHHHHHHHHHCHHHHHHHHHHH
HEGKSIRSVIHFDKK
HCCHHHHHHHHCCCC
>Mature Secondary Structure
MEMIKTRAAVAWGPNQPLKIEEVDLMPPQKGEVLVRIVATGVCHTDAYTLSGADPEGVFP
CCCHHCCEEEEECCCCCCEEEEECCCCCCCCCEEEEEEECCCCCCCCEEECCCCCCCCCH
AILGHEGGGVVEAIGEGVTSVAVGDHVIPLYTPECGECKFCKSGKTNLCQAIRATQGKGL
HEECCCCCCHHHHHHCCHHEEEECCEEEEEECCCCCCCCCCCCCCHHHHHHHHHCCCCCC
MPDGTTRFFKDGKPIFHYMGTSTFSEYTVVPEISLAKISKEAPLEEVCLLGCGVTTGMGA
CCCCCHHHHHCCCCEEEECCCCCCCCEEECCCCHHHHHCCCCCHHHHHHHHCCHHHCCHH
VINTAKVQKGDTVAIFGLGGIGLSAIIGAQMAGAGRIIGIDINTSKFDLARKLGATDLIN
HEEHHEECCCCEEEEEEECCHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHCCCCCCCC
PKDYDKPIQDVIVELTDGGVDFSFECIGNVNVMRSALECCHKGWGESVIIGVAGAGQEIS
CCCCCCCHHHHHEEECCCCCCEEEEEECCHHHHHHHHHHHHCCCCCEEEEEEECCCCCCC
TRPFQLVTGRVWRGSAFGGVKGRSQLPGIVQRYMDGEFALNDFITHTMGLEQINEAFDLM
CCCEEEEECEEECCCCCCCCCCCCCCHHHHHHHCCCCHHHHHHHHHHHCHHHHHHHHHHH
HEGKSIRSVIHFDKK
HCCHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]