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The map label for this gene is yneJ [H]

Identifier: 157369801

GI number: 157369801

Start: 1697401

End: 1698318

Strand: Reverse

Name: yneJ [H]

Synonym: Spro_1558

Alternate gene names: 157369801

Gene position: 1698318-1697401 (Counterclockwise)

Preceding gene: 157369809

Following gene: 157369800

Centisome position: 31.17

GC content: 59.48

Gene sequence:

>918_bases
ATGGATCTCACCCAGCTCCGCATGTTCTGCAGCGTCGCCGAAACCGGCTCCGTTGCCCGTGCCGCCGAGCAGTTGCACCG
CGTACCTTCGAATCTGACCACCCGCCTGCGTCAGCTCGAACAGGAACTGGGCACCGATCTGTTCATCCGCGAAAAACAGC
GCCTGCGCCTTTCGCCGATGGGCCATAACTTTCTCTGCTACGCCAACCGCATTCTGGCCCTGAGTGAAGAAGCGATGAGC
ATCACCCATGCCGGTGAGCCGGCAGGTAACTTCGCCATAGGTTCGATGGAAAGCACCGCCGCCACCCGCTTGCCAACGTT
ACTGGCAGCCTATCACCAACGTTTCTCACAGGTGTCACTGTCGCTGACCACCGGTACCTCCGGCGAGATCACCGAACGGG
TACGCGCCGGTACGCTGGCGGCGGCACTGGTCGATGGGCCGGTACAGTATGACGAACTCAATGGCTGTATCGCTTTCCCC
GAACATATGGTGGTGATCTCATGCCTCGATCACCTGCCGATCCATAGCGCCAAAGACGCCAAGGGCGAAACGCTGTTTGC
CTTCCGCGCCAGTTGCTCTTACCGCCTGCGGCTGGAGTCCTGGTTCAAGCGCGAAGGTGTATTGCCGGGCCAGGTGATGG
AAATCCAGTCTTACCACGCCATGCTGGCCTGCGTCGCCAGCGGCGCCGGGCTGGCGATGATCCCGCATTCGGTGCTGAGC
CTGCTGCCAGGCCATGAACGGGTAAAAGTGCATACTCTTCCGGGCGATATCGCCCAAACCGCCACCTGGTTACTGTGGCG
GCGTGATGCCTTCGGCCCGAACGTTCGCGCCCTGAAAGAATTAATTATTGAACAGGCAGAAACCCTCTCGGTTGAAAATA
CTGCCTGCGATTTACCCAATGCTATCGACATAGCCTGA

Upstream 100 bases:

>100_bases
TATCCAGACTGTTAAAAGAAAGTTGCCAGTATCATGCCGCAGTATTAAACTCACAAAAACTGAATAATACTCACCAAGTT
CATCACGAAAAGAGAAGACA

Downstream 100 bases:

>100_bases
TTAAAAATAGACCACTGGAGACTATGATGGAAATGATCAAAACCCGTGCCGCCGTTGCCTGGGGCCCGAACCAGCCGCTG
AAAATCGAAGAAGTGGATCT

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 305; Mature: 305

Protein sequence:

>305_residues
MDLTQLRMFCSVAETGSVARAAEQLHRVPSNLTTRLRQLEQELGTDLFIREKQRLRLSPMGHNFLCYANRILALSEEAMS
ITHAGEPAGNFAIGSMESTAATRLPTLLAAYHQRFSQVSLSLTTGTSGEITERVRAGTLAAALVDGPVQYDELNGCIAFP
EHMVVISCLDHLPIHSAKDAKGETLFAFRASCSYRLRLESWFKREGVLPGQVMEIQSYHAMLACVASGAGLAMIPHSVLS
LLPGHERVKVHTLPGDIAQTATWLLWRRDAFGPNVRALKELIIEQAETLSVENTACDLPNAIDIA

Sequences:

>Translated_305_residues
MDLTQLRMFCSVAETGSVARAAEQLHRVPSNLTTRLRQLEQELGTDLFIREKQRLRLSPMGHNFLCYANRILALSEEAMS
ITHAGEPAGNFAIGSMESTAATRLPTLLAAYHQRFSQVSLSLTTGTSGEITERVRAGTLAAALVDGPVQYDELNGCIAFP
EHMVVISCLDHLPIHSAKDAKGETLFAFRASCSYRLRLESWFKREGVLPGQVMEIQSYHAMLACVASGAGLAMIPHSVLS
LLPGHERVKVHTLPGDIAQTATWLLWRRDAFGPNVRALKELIIEQAETLSVENTACDLPNAIDIA
>Mature_305_residues
MDLTQLRMFCSVAETGSVARAAEQLHRVPSNLTTRLRQLEQELGTDLFIREKQRLRLSPMGHNFLCYANRILALSEEAMS
ITHAGEPAGNFAIGSMESTAATRLPTLLAAYHQRFSQVSLSLTTGTSGEITERVRAGTLAAALVDGPVQYDELNGCIAFP
EHMVVISCLDHLPIHSAKDAKGETLFAFRASCSYRLRLESWFKREGVLPGQVMEIQSYHAMLACVASGAGLAMIPHSVLS
LLPGHERVKVHTLPGDIAQTATWLLWRRDAFGPNVRALKELIIEQAETLSVENTACDLPNAIDIA

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787806, Length=282, Percent_Identity=50, Blast_Score=297, Evalue=6e-82,
Organism=Escherichia coli, GI1788887, Length=298, Percent_Identity=25.1677852348993, Blast_Score=95, Evalue=5e-21,
Organism=Escherichia coli, GI1790208, Length=264, Percent_Identity=27.2727272727273, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI157672245, Length=273, Percent_Identity=23.8095238095238, Blast_Score=71, Evalue=7e-14,
Organism=Escherichia coli, GI1788296, Length=246, Percent_Identity=25.2032520325203, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1790262, Length=248, Percent_Identity=25, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1787530, Length=283, Percent_Identity=23.321554770318, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1788297, Length=242, Percent_Identity=25.6198347107438, Blast_Score=65, Evalue=5e-12,
Organism=Escherichia coli, GI1787601, Length=287, Percent_Identity=23.6933797909408, Blast_Score=62, Evalue=3e-11,
Organism=Escherichia coli, GI87081904, Length=173, Percent_Identity=27.7456647398844, Blast_Score=62, Evalue=3e-11,
Organism=Escherichia coli, GI1786401, Length=169, Percent_Identity=28.9940828402367, Blast_Score=61, Evalue=1e-10,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33406; Mature: 33406

Theoretical pI: Translated: 6.58; Mature: 6.58

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDLTQLRMFCSVAETGSVARAAEQLHRVPSNLTTRLRQLEQELGTDLFIREKQRLRLSPM
CCHHHHHHHHHHHHCCHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCEEEECHHHCEECCC
GHNFLCYANRILALSEEAMSITHAGEPAGNFAIGSMESTAATRLPTLLAAYHQRFSQVSL
CCCEEHHHHHHHHHHHHHHHHCCCCCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHEEE
SLTTGTSGEITERVRAGTLAAALVDGPVQYDELNGCIAFPEHMVVISCLDHLPIHSAKDA
EEECCCCCHHHHHHHHHHHHHHHHCCCCCHHHCCCEEECHHHHHHHHHHHCCCCCCCCCC
KGETLFAFRASCSYRLRLESWFKREGVLPGQVMEIQSYHAMLACVASGAGLAMIPHSVLS
CCCEEEEEECCCCEEEHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
LLPGHERVKVHTLPGDIAQTATWLLWRRDAFGPNVRALKELIIEQAETLSVENTACDLPN
HCCCCCEEEEEECCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCEECCCCCCCCC
AIDIA
CCCCC
>Mature Secondary Structure
MDLTQLRMFCSVAETGSVARAAEQLHRVPSNLTTRLRQLEQELGTDLFIREKQRLRLSPM
CCHHHHHHHHHHHHCCHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCEEEECHHHCEECCC
GHNFLCYANRILALSEEAMSITHAGEPAGNFAIGSMESTAATRLPTLLAAYHQRFSQVSL
CCCEEHHHHHHHHHHHHHHHHCCCCCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHEEE
SLTTGTSGEITERVRAGTLAAALVDGPVQYDELNGCIAFPEHMVVISCLDHLPIHSAKDA
EEECCCCCHHHHHHHHHHHHHHHHCCCCCHHHCCCEEECHHHHHHHHHHHCCCCCCCCCC
KGETLFAFRASCSYRLRLESWFKREGVLPGQVMEIQSYHAMLACVASGAGLAMIPHSVLS
CCCEEEEEECCCCEEEHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
LLPGHERVKVHTLPGDIAQTATWLLWRRDAFGPNVRALKELIIEQAETLSVENTACDLPN
HCCCCCEEEEEECCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCEECCCCCCCCC
AIDIA
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]