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The map label for this gene is ybfF [H]

Identifier: 157369482

GI number: 157369482

Start: 1363583

End: 1364356

Strand: Reverse

Name: ybfF [H]

Synonym: Spro_1239

Alternate gene names: 157369482

Gene position: 1364356-1363583 (Counterclockwise)

Preceding gene: 157369487

Following gene: 157369481

Centisome position: 25.04

GC content: 57.75

Gene sequence:

>774_bases
ATGAACTTCGCCATGAAATTACATTATCAACTGCTGGCCTGCGAGTCCGATGCTCTGCCGGTTGTCCTGATCCACGGGCT
GTTTGGCAATCTGGACAATCTGGGCGTGCTGGCGCGCGATCTGAACCAACAGCACAGCGTGATTAAAGTCGATCTGCGCA
ACCACGGCCTGTCGCCACGTTCCGAGGTGATGACCTACCCGGAAATGGCGCGTGATCTGCTGGCGCTGCTGGATGATTTG
CAGTTGGACAAGGTCATTGTGATTGGACACTCGATGGGCGGCAAAGCGGCGATGGCGCTGACGGCAATCGCACCTGAGCG
AGTGGAAAAACTGATCGTTATTGACGTGGCACCGGTGGATTATCAAACCCGTCGCCACGACGAGATTTTTGTCGCCTTAC
AAGCCGTAAGTGCGGCCGGTATCACCCAACGCCAGCAGGCGGCAGAACTGATGCGCGACTACCTGAAGGAAGAAGGAGTG
ATCCAGTTCCTGCTGAAATCCTTCCACCAGGGCGAATGGCGCTTTAACCTGCCGGTGCTGATCGAACAATACGAAAACAT
CACCGGCTGGCAGGAGGTTCCCGCCTGGCCACATCCCACATTATTCATTCGTGGTGGTTTGTCGCCCTACGTGCAGGACA
GCTACCGCGCTGATATTGCCCGCCAGTTCCCGCAGGCTCGCGCGCACGTGGTCGCCGGCACCGGGCACTGGGTTCATGCG
GAAAAACCGGAGGCGGTGCTGCGCGCAATTCACCGTTTTCTCGGCGAAGCCTGA

Upstream 100 bases:

>100_bases
GGGGTGAGCTAGTGCAGGTAACAACGCTGCGGCTTGAAAGGCGACGGGTATTTTCATCGTGTGTTTTAATCATGATACCC
TAATCTGAATCTCACTCAGT

Downstream 100 bases:

>100_bases
GTCGACCAGCCCTTTAGCCTGATGCGTAGAGCCGCCTAAAACAATCATCTGGTGAAAAAACGCGCTAAAGATAACGGCTT
AGCACGTTTTCGGCTCAGTT

Product: alpha/beta hydrolase fold domain-containing protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 257; Mature: 257

Protein sequence:

>257_residues
MNFAMKLHYQLLACESDALPVVLIHGLFGNLDNLGVLARDLNQQHSVIKVDLRNHGLSPRSEVMTYPEMARDLLALLDDL
QLDKVIVIGHSMGGKAAMALTAIAPERVEKLIVIDVAPVDYQTRRHDEIFVALQAVSAAGITQRQQAAELMRDYLKEEGV
IQFLLKSFHQGEWRFNLPVLIEQYENITGWQEVPAWPHPTLFIRGGLSPYVQDSYRADIARQFPQARAHVVAGTGHWVHA
EKPEAVLRAIHRFLGEA

Sequences:

>Translated_257_residues
MNFAMKLHYQLLACESDALPVVLIHGLFGNLDNLGVLARDLNQQHSVIKVDLRNHGLSPRSEVMTYPEMARDLLALLDDL
QLDKVIVIGHSMGGKAAMALTAIAPERVEKLIVIDVAPVDYQTRRHDEIFVALQAVSAAGITQRQQAAELMRDYLKEEGV
IQFLLKSFHQGEWRFNLPVLIEQYENITGWQEVPAWPHPTLFIRGGLSPYVQDSYRADIARQFPQARAHVVAGTGHWVHA
EKPEAVLRAIHRFLGEA
>Mature_257_residues
MNFAMKLHYQLLACESDALPVVLIHGLFGNLDNLGVLARDLNQQHSVIKVDLRNHGLSPRSEVMTYPEMARDLLALLDDL
QLDKVIVIGHSMGGKAAMALTAIAPERVEKLIVIDVAPVDYQTRRHDEIFVALQAVSAAGITQRQQAAELMRDYLKEEGV
IQFLLKSFHQGEWRFNLPVLIEQYENITGWQEVPAWPHPTLFIRGGLSPYVQDSYRADIARQFPQARAHVVAGTGHWVHA
EKPEAVLRAIHRFLGEA

Specific function: Displays esterase activity toward palmitoyl-CoA, malonyl-CoA and pNP-butyrate [H]

COG id: COG0596

COG function: function code R; Predicted hydrolases or acyltransferases (alpha/beta hydrolase superfamily)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dmpD/todF/xylF esterase family [H]

Homologues:

Organism=Homo sapiens, GI23200008, Length=262, Percent_Identity=38.1679389312977, Blast_Score=159, Evalue=2e-39,
Organism=Homo sapiens, GI23200012, Length=257, Percent_Identity=38.1322957198444, Blast_Score=155, Evalue=4e-38,
Organism=Homo sapiens, GI223941839, Length=253, Percent_Identity=32.8063241106719, Blast_Score=119, Evalue=2e-27,
Organism=Escherichia coli, GI1786902, Length=252, Percent_Identity=63.4920634920635, Blast_Score=344, Evalue=3e-96,
Organism=Caenorhabditis elegans, GI17508535, Length=252, Percent_Identity=34.5238095238095, Blast_Score=151, Evalue=4e-37,
Organism=Caenorhabditis elegans, GI17507063, Length=262, Percent_Identity=32.0610687022901, Blast_Score=110, Evalue=9e-25,
Organism=Saccharomyces cerevisiae, GI6321468, Length=263, Percent_Identity=33.0798479087452, Blast_Score=112, Evalue=7e-26,
Organism=Drosophila melanogaster, GI24640348, Length=255, Percent_Identity=30.1960784313725, Blast_Score=116, Evalue=2e-26,
Organism=Drosophila melanogaster, GI24646055, Length=256, Percent_Identity=26.953125, Blast_Score=82, Evalue=3e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000073 [H]

Pfam domain/function: PF00561 Abhydrolase_1 [H]

EC number: 3.1.-.- [C]

Molecular weight: Translated: 28894; Mature: 28894

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS00120 LIPASE_SER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNFAMKLHYQLLACESDALPVVLIHGLFGNLDNLGVLARDLNQQHSVIKVDLRNHGLSPR
CCCCCEEEEEEEEECCCCCCCEEEEHHHCCCCHHHHHHHHCCCCCCEEEEEHHHCCCCCH
SEVMTYPEMARDLLALLDDLQLDKVIVIGHSMGGKAAMALTAIAPERVEKLIVIDVAPVD
HHHHCCHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHCHHHHCEEEEEEECCCC
YQTRRHDEIFVALQAVSAAGITQRQQAAELMRDYLKEEGVIQFLLKSFHQGEWRFNLPVL
CCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCHHHHHHHHHHCCCCEEECCCHH
IEQYENITGWQEVPAWPHPTLFIRGGLSPYVQDSYRADIARQFPQARAHVVAGTGHWVHA
HHHHCCCCCCHHCCCCCCCEEEEECCCCHHHHHHHHHHHHHHCCHHHEEEEECCCCEEEC
EKPEAVLRAIHRFLGEA
CCHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNFAMKLHYQLLACESDALPVVLIHGLFGNLDNLGVLARDLNQQHSVIKVDLRNHGLSPR
CCCCCEEEEEEEEECCCCCCCEEEEHHHCCCCHHHHHHHHCCCCCCEEEEEHHHCCCCCH
SEVMTYPEMARDLLALLDDLQLDKVIVIGHSMGGKAAMALTAIAPERVEKLIVIDVAPVD
HHHHCCHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHCHHHHCEEEEEEECCCC
YQTRRHDEIFVALQAVSAAGITQRQQAAELMRDYLKEEGVIQFLLKSFHQGEWRFNLPVL
CCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCHHHHHHHHHHCCCCEEECCCHH
IEQYENITGWQEVPAWPHPTLFIRGGLSPYVQDSYRADIARQFPQARAHVVAGTGHWVHA
HHHHCCCCCCHHCCCCCCCEEEEECCCCHHHHHHHHHHHHHHCCHHHEEEEECCCCEEEC
EKPEAVLRAIHRFLGEA
CCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503; 10493123 [H]