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The map label for this gene is tgt [H]

Identifier: 157369304

GI number: 157369304

Start: 1167916

End: 1169040

Strand: Direct

Name: tgt [H]

Synonym: Spro_1061

Alternate gene names: 157369304

Gene position: 1167916-1169040 (Clockwise)

Preceding gene: 157369303

Following gene: 157369305

Centisome position: 21.43

GC content: 53.07

Gene sequence:

>1125_bases
GTGAAGTACGAATTAGACACAACCGATGGCCGCGCCCGCCGTGGCCGCCTGATTTTTGAACGTGGCGTGGTGGAAACCCC
AGCCTTTATGCCGGTCGGCACCTACGGCACGGTAAAGGGCATGACGCCGGAAGAAGTGAAAGAGACCGGTGCGCAGATCC
TGCTGGGCAACACCTTCCACCTGTGGCTGCGCCCAGGGCAGGAGATCATGAAGCTGCATGGCGATCTGCATGATTTCATG
CAGTGGCATGGCCCGATCCTCACCGATTCAGGCGGCTTCCAGGTGTTCAGCCTGGGCGCGATGCGCAAGATTAAAGAGGA
AGGCGTACATTTCCGTAACCCGATTAACGGCGATAAAGTGTTCCTCAGCCCGGAAAAATCGATGGAAATCCAGTATGACC
TGGGGTCTGACATCGTGATGATCTTCGACGAATGTACGCCGTACCCGGCAGACTGGGACTACGCCAAAAGCTCGATGGAA
ATGTCTCTGCGCTGGGCGCAGCGCAGCCGTCAGCGTTTTGACGAACTGAATAACAAGAACGCTTTATTCGGCATTATTCA
GGGCGGTGTTTACGAAGATTTACGTGATGTATCAGTAAAAGGGCTGGTGGATATCGGTTTTGATGGTTACGCTGTGGGCG
GCTTGGCAGTAGGTGAGCCAAAAGAGGATATGCATCGCATTCTTGAACATGTTTGCCCGCAAATTCCGGAAGATAAGCCA
CGTTACCTGATGGGCGTTGGCAAACCGGAAGATCTGGTTGAAGGCGTGCGCCGCGGTATCGATATGTTCGACTGCGTGAT
GCCAACGCGTAATGCCCGTAACGGCCATCTGTTCGTGACTGACGGTGTGGTAAAAATCCGTAATGCCAAGCACAAGGATG
ATACTTCTCCGCTGGATAAAGACTGTGATTGCTACACGTGTCGCAATTACAGCCGCGCCTACTTGCATCATCTCGATCGT
TGCAACGAAATACTCGGTGCCCGATTGAACACCATTCATAACCTGCGTCACTACCAACGCCTGATGGCGGGTTTACGCGA
GGCCATTGAACAGGGTAAATTAGAGCTGTTTGTTGCGGATTTCTACGGTCGGATCGGTAAACCGGTTCCACCTTTAAACG
CTTGA

Upstream 100 bases:

>100_bases
GGCGGAGCAAGAGTCCGTCGGCGCCTGATTTCCTGGGCTGCGCATCTGGCGCAACCCTGTTTTAACGACATCAGACTGTT
TCTCTGATGCTGGAGGCTTT

Downstream 100 bases:

>100_bases
ATTAATAATTGATAACTTAATGAGGGAATTTCAATGAGCTTTTTCATTTCTGACGCCGTCGCATCCGCAGGAGCTCCGTC
TCAGGGAAGCCCGTACTCTC

Product: queuine tRNA-ribosyltransferase

Products: NA

Alternate protein names: Guanine insertion enzyme; tRNA-guanine transglycosylase [H]

Number of amino acids: Translated: 374; Mature: 374

Protein sequence:

>374_residues
MKYELDTTDGRARRGRLIFERGVVETPAFMPVGTYGTVKGMTPEEVKETGAQILLGNTFHLWLRPGQEIMKLHGDLHDFM
QWHGPILTDSGGFQVFSLGAMRKIKEEGVHFRNPINGDKVFLSPEKSMEIQYDLGSDIVMIFDECTPYPADWDYAKSSME
MSLRWAQRSRQRFDELNNKNALFGIIQGGVYEDLRDVSVKGLVDIGFDGYAVGGLAVGEPKEDMHRILEHVCPQIPEDKP
RYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGHLFVTDGVVKIRNAKHKDDTSPLDKDCDCYTCRNYSRAYLHHLDR
CNEILGARLNTIHNLRHYQRLMAGLREAIEQGKLELFVADFYGRIGKPVPPLNA

Sequences:

>Translated_374_residues
MKYELDTTDGRARRGRLIFERGVVETPAFMPVGTYGTVKGMTPEEVKETGAQILLGNTFHLWLRPGQEIMKLHGDLHDFM
QWHGPILTDSGGFQVFSLGAMRKIKEEGVHFRNPINGDKVFLSPEKSMEIQYDLGSDIVMIFDECTPYPADWDYAKSSME
MSLRWAQRSRQRFDELNNKNALFGIIQGGVYEDLRDVSVKGLVDIGFDGYAVGGLAVGEPKEDMHRILEHVCPQIPEDKP
RYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGHLFVTDGVVKIRNAKHKDDTSPLDKDCDCYTCRNYSRAYLHHLDR
CNEILGARLNTIHNLRHYQRLMAGLREAIEQGKLELFVADFYGRIGKPVPPLNA
>Mature_374_residues
MKYELDTTDGRARRGRLIFERGVVETPAFMPVGTYGTVKGMTPEEVKETGAQILLGNTFHLWLRPGQEIMKLHGDLHDFM
QWHGPILTDSGGFQVFSLGAMRKIKEEGVHFRNPINGDKVFLSPEKSMEIQYDLGSDIVMIFDECTPYPADWDYAKSSME
MSLRWAQRSRQRFDELNNKNALFGIIQGGVYEDLRDVSVKGLVDIGFDGYAVGGLAVGEPKEDMHRILEHVCPQIPEDKP
RYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGHLFVTDGVVKIRNAKHKDDTSPLDKDCDCYTCRNYSRAYLHHLDR
CNEILGARLNTIHNLRHYQRLMAGLREAIEQGKLELFVADFYGRIGKPVPPLNA

Specific function: Exchanges the guanine residue with 7-aminomethyl-7- deazaguanine in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr). After this exchange, a cyclopentendiol moiety is attached to the 7-aminomethyl group of 7-deazaguanine, resulting in the hyper

COG id: COG0343

COG function: function code J; Queuine/archaeosine tRNA-ribosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queuine tRNA-ribosyltransferase family [H]

Homologues:

Organism=Homo sapiens, GI13654276, Length=368, Percent_Identity=42.3913043478261, Blast_Score=300, Evalue=1e-81,
Organism=Homo sapiens, GI13375872, Length=227, Percent_Identity=27.3127753303965, Blast_Score=103, Evalue=3e-22,
Organism=Escherichia coli, GI1786607, Length=374, Percent_Identity=89.572192513369, Blast_Score=707, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17544680, Length=375, Percent_Identity=42.1333333333333, Blast_Score=295, Evalue=3e-80,
Organism=Caenorhabditis elegans, GI25146502, Length=171, Percent_Identity=32.1637426900585, Blast_Score=91, Evalue=8e-19,
Organism=Drosophila melanogaster, GI19920510, Length=371, Percent_Identity=45.2830188679245, Blast_Score=321, Evalue=6e-88,
Organism=Drosophila melanogaster, GI21355311, Length=253, Percent_Identity=29.6442687747036, Blast_Score=101, Evalue=8e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004803
- InterPro:   IPR002616 [H]

Pfam domain/function: PF01702 TGT [H]

EC number: =2.4.2.29 [H]

Molecular weight: Translated: 42494; Mature: 42494

Theoretical pI: Translated: 6.57; Mature: 6.57

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKYELDTTDGRARRGRLIFERGVVETPAFMPVGTYGTVKGMTPEEVKETGAQILLGNTFH
CCEECCCCCCCHHCCCEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCEEEECCEEE
LWLRPGQEIMKLHGDLHDFMQWHGPILTDSGGFQVFSLGAMRKIKEEGVHFRNPINGDKV
EEECCCHHHHHHHHHHHHHHHHCCCEEECCCCCEEEEHHHHHHHHHCCCEECCCCCCCEE
FLSPEKSMEIQYDLGSDIVMIFDECTPYPADWDYAKSSMEMSLRWAQRSRQRFDELNNKN
EECCCCCCEEEEECCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
ALFGIIQGGVYEDLRDVSVKGLVDIGFDGYAVGGLAVGEPKEDMHRILEHVCPQIPEDKP
EEEEEECCCHHHHHHHCCCCEEEEECCCCEEECCEECCCCHHHHHHHHHHHCCCCCCCCC
RYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGHLFVTDGVVKIRNAKHKDDTSPLDK
CEEEECCCHHHHHHHHHHCCHHHHHHCCCCCCCCCEEEEECCEEEECCCCCCCCCCCCCC
DCDCYTCRNYSRAYLHHLDRCNEILGARLNTIHNLRHYQRLMAGLREAIEQGKLELFVAD
CCCCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEH
FYGRIGKPVPPLNA
HHHHCCCCCCCCCC
>Mature Secondary Structure
MKYELDTTDGRARRGRLIFERGVVETPAFMPVGTYGTVKGMTPEEVKETGAQILLGNTFH
CCEECCCCCCCHHCCCEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCEEEECCEEE
LWLRPGQEIMKLHGDLHDFMQWHGPILTDSGGFQVFSLGAMRKIKEEGVHFRNPINGDKV
EEECCCHHHHHHHHHHHHHHHHCCCEEECCCCCEEEEHHHHHHHHHCCCEECCCCCCCEE
FLSPEKSMEIQYDLGSDIVMIFDECTPYPADWDYAKSSMEMSLRWAQRSRQRFDELNNKN
EECCCCCCEEEEECCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
ALFGIIQGGVYEDLRDVSVKGLVDIGFDGYAVGGLAVGEPKEDMHRILEHVCPQIPEDKP
EEEEEECCCHHHHHHHCCCCEEEEECCCCEEECCEECCCCHHHHHHHHHHHCCCCCCCCC
RYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGHLFVTDGVVKIRNAKHKDDTSPLDK
CEEEECCCHHHHHHHHHHCCHHHHHHCCCCCCCCCEEEEECCEEEECCCCCCCCCCCCCC
DCDCYTCRNYSRAYLHHLDRCNEILGARLNTIHNLRHYQRLMAGLREAIEQGKLELFVAD
CCCCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEH
FYGRIGKPVPPLNA
HHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA