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The map label for this gene is queA

Identifier: 157369303

GI number: 157369303

Start: 1166773

End: 1167843

Strand: Direct

Name: queA

Synonym: Spro_1060

Alternate gene names: 157369303

Gene position: 1166773-1167843 (Clockwise)

Preceding gene: 157369292

Following gene: 157369304

Centisome position: 21.41

GC content: 59.66

Gene sequence:

>1071_bases
ATGCGCGTCGCCGATTTTTCGTTTGAACTTCCCGAGTCCCTGATTGCCCACTATCCCCAGCCTGAACGCAGCGGTTGCCG
TTTGCTGCAGTTGGACGGGCCGAGCGGGGAGCTTAAGCATGGCGTATTCACCGATCTGCTGGATAACCTGGAAGCCGGCG
ATCTGCTGGTGTTCAACAATACCCGGGTGATCCCGGCGCGCATGTTTGGCCGCAAGGTCAGCGGCGGCAAGATTGAAGTG
TTGGTCGAGCGCGTGCTGGACGACCATCGGGTATTGGCGCATGTCCGCGCCTCGAAGGCCCCGAAACCGGGCACCGACCT
GCTGCTGGGTGACGACGAAAGCATCGCCGCCACTATGGTCGCGCGCCACGATACGCTGTTCGAACTGCGCTTCAACGATG
AACGCGACGTCTTTACTATCCTTAATGCCGTTGGCCATATGCCGCTGCCGCCGTATATCGATCGTCCGGATGAAGACGCC
GACCGCGAACTTTATCAGACGGTCTACAGCGAAAAACCGGGTGCGGTAGCTGCACCAACCGCCGGGCTGCATTTCGACGA
GCCGCTGTTGGCTGCGCTGCGCGAGAAAGGCATCGAGATGGCGTTTGTCACCCTGCACGTGGGCGCGGGCACCTTCCAGC
CGGTGCGGGTGGAAACCATCGAAGAGCACGTCATGCATGCCGAATACGCAGAGGTGCCGCAAGAGGTGGTTGACGCCGTA
TTGGCCTGCAAAGCGCGCGGCAAGCGTGTGGTGGCTGTCGGCACCACCTCGGTGCGTTCACTTGAAAGTGCCGCCAACGC
CAGCAAAGAGGCATTGATTGCGCCGTTCTTCGACGACACCAGCATCTTTATCTTCCCGGGTTACCACTATCAGGTGGTTG
ACGCACTGGTGACCAACTTCCACCTGCCGGAGTCGACGCTGATTATGCTGGTTTCCGCCTTTGCCGGTTATAAAAACACC
ATGCATGCCTATCAGCAGGCGGTGGCCGAGCAGTACCGTTTCTTTAGCTATGGCGATGCGATGTTTATCAGCCGTAACCC
GCAGGCGGAGCAAGAGTCCGTCGGCGCCTGA

Upstream 100 bases:

>100_bases
CATGCAAATCCTCCTTATTTCTTGCCGCTGACCGCCCGCAGCTTTAGACTAGGCCGCCTGTTTTTTACCAACCAGTCCTA
TATTGAAGAAGTGAATAGCC

Downstream 100 bases:

>100_bases
TTTCCTGGGCTGCGCATCTGGCGCAACCCTGTTTTAACGACATCAGACTGTTTCTCTGATGCTGGAGGCTTTGTGAAGTA
CGAATTAGACACAACCGATG

Product: S-adenosylmethionine:tRNA ribosyltransferase-isomerase

Products: NA

Alternate protein names: Queuosine biosynthesis protein queA

Number of amino acids: Translated: 356; Mature: 356

Protein sequence:

>356_residues
MRVADFSFELPESLIAHYPQPERSGCRLLQLDGPSGELKHGVFTDLLDNLEAGDLLVFNNTRVIPARMFGRKVSGGKIEV
LVERVLDDHRVLAHVRASKAPKPGTDLLLGDDESIAATMVARHDTLFELRFNDERDVFTILNAVGHMPLPPYIDRPDEDA
DRELYQTVYSEKPGAVAAPTAGLHFDEPLLAALREKGIEMAFVTLHVGAGTFQPVRVETIEEHVMHAEYAEVPQEVVDAV
LACKARGKRVVAVGTTSVRSLESAANASKEALIAPFFDDTSIFIFPGYHYQVVDALVTNFHLPESTLIMLVSAFAGYKNT
MHAYQQAVAEQYRFFSYGDAMFISRNPQAEQESVGA

Sequences:

>Translated_356_residues
MRVADFSFELPESLIAHYPQPERSGCRLLQLDGPSGELKHGVFTDLLDNLEAGDLLVFNNTRVIPARMFGRKVSGGKIEV
LVERVLDDHRVLAHVRASKAPKPGTDLLLGDDESIAATMVARHDTLFELRFNDERDVFTILNAVGHMPLPPYIDRPDEDA
DRELYQTVYSEKPGAVAAPTAGLHFDEPLLAALREKGIEMAFVTLHVGAGTFQPVRVETIEEHVMHAEYAEVPQEVVDAV
LACKARGKRVVAVGTTSVRSLESAANASKEALIAPFFDDTSIFIFPGYHYQVVDALVTNFHLPESTLIMLVSAFAGYKNT
MHAYQQAVAEQYRFFSYGDAMFISRNPQAEQESVGA
>Mature_356_residues
MRVADFSFELPESLIAHYPQPERSGCRLLQLDGPSGELKHGVFTDLLDNLEAGDLLVFNNTRVIPARMFGRKVSGGKIEV
LVERVLDDHRVLAHVRASKAPKPGTDLLLGDDESIAATMVARHDTLFELRFNDERDVFTILNAVGHMPLPPYIDRPDEDA
DRELYQTVYSEKPGAVAAPTAGLHFDEPLLAALREKGIEMAFVTLHVGAGTFQPVRVETIEEHVMHAEYAEVPQEVVDAV
LACKARGKRVVAVGTTSVRSLESAANASKEALIAPFFDDTSIFIFPGYHYQVVDALVTNFHLPESTLIMLVSAFAGYKNT
MHAYQQAVAEQYRFFSYGDAMFISRNPQAEQESVGA

Specific function: Transfers and isomerizes the ribose moiety from AdoMet to the 7-aminomethyl group of 7-deazaguanine (preQ1-tRNA) to give epoxyqueuosine (oQ-tRNA)

COG id: COG0809

COG function: function code J; S-adenosylmethionine:tRNA-ribosyltransferase-isomerase (queuine synthetase)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queA family

Homologues:

Organism=Escherichia coli, GI1786606, Length=355, Percent_Identity=81.9718309859155, Blast_Score=610, Evalue=1e-176,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): QUEA_SERP5 (A8GAM4)

Other databases:

- EMBL:   CP000826
- RefSeq:   YP_001477292.1
- ProteinModelPortal:   A8GAM4
- SMR:   A8GAM4
- STRING:   A8GAM4
- GeneID:   5606758
- GenomeReviews:   CP000826_GR
- KEGG:   spe:Spro_1060
- eggNOG:   COG0809
- HOGENOM:   HBG286076
- OMA:   ITRNPAP
- ProtClustDB:   PRK00147
- BioCyc:   SPRO399741:SPRO_1060-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00113
- InterPro:   IPR003699
- TIGRFAMs:   TIGR00113

Pfam domain/function: PF02547 Queuosine_synth; SSF111337 Queuosine_synth

EC number: 5.-.-.- [C]

Molecular weight: Translated: 39331; Mature: 39331

Theoretical pI: Translated: 4.87; Mature: 4.87

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVADFSFELPESLIAHYPQPERSGCRLLQLDGPSGELKHGVFTDLLDNLEAGDLLVFNN
CCCCCCCCCCCHHHHHCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHCCCCCCEEEECC
TRVIPARMFGRKVSGGKIEVLVERVLDDHRVLAHVRASKAPKPGTDLLLGDDESIAATMV
CEEEEHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCCCHHHHHHH
ARHDTLFELRFNDERDVFTILNAVGHMPLPPYIDRPDEDADRELYQTVYSEKPGAVAAPT
HHCCEEEEEEECCCCHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCEECCC
AGLHFDEPLLAALREKGIEMAFVTLHVGAGTFQPVRVETIEEHVMHAEYAEVPQEVVDAV
CCCCCCCHHHHHHHHCCCEEEEEEEEECCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHH
LACKARGKRVVAVGTTSVRSLESAANASKEALIAPFFDDTSIFIFPGYHYQVVDALVTNF
HHHHCCCCEEEEECCHHHHHHHHHHCCCCCEEEEEEECCCEEEEECCCHHHHHHHHHHHC
HLPESTLIMLVSAFAGYKNTMHAYQQAVAEQYRFFSYGDAMFISRNPQAEQESVGA
CCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHCCCC
>Mature Secondary Structure
MRVADFSFELPESLIAHYPQPERSGCRLLQLDGPSGELKHGVFTDLLDNLEAGDLLVFNN
CCCCCCCCCCCHHHHHCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHCCCCCCEEEECC
TRVIPARMFGRKVSGGKIEVLVERVLDDHRVLAHVRASKAPKPGTDLLLGDDESIAATMV
CEEEEHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCCCHHHHHHH
ARHDTLFELRFNDERDVFTILNAVGHMPLPPYIDRPDEDADRELYQTVYSEKPGAVAAPT
HHCCEEEEEEECCCCHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCEECCC
AGLHFDEPLLAALREKGIEMAFVTLHVGAGTFQPVRVETIEEHVMHAEYAEVPQEVVDAV
CCCCCCCHHHHHHHHCCCEEEEEEEEECCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHH
LACKARGKRVVAVGTTSVRSLESAANASKEALIAPFFDDTSIFIFPGYHYQVVDALVTNF
HHHHCCCCEEEEECCHHHHHHHHHHCCCCCEEEEEEECCCEEEEECCCHHHHHHHHHHHC
HLPESTLIMLVSAFAGYKNTMHAYQQAVAEQYRFFSYGDAMFISRNPQAEQESVGA
CCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Isomerases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA