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The map label for this gene is ycdT [H]

Identifier: 157368890

GI number: 157368890

Start: 710142

End: 711215

Strand: Reverse

Name: ycdT [H]

Synonym: Spro_0645

Alternate gene names: 157368890

Gene position: 711215-710142 (Counterclockwise)

Preceding gene: 157368892

Following gene: 157368882

Centisome position: 13.05

GC content: 48.7

Gene sequence:

>1074_bases
ATGAACGCGCGTTCTTATCAGGAACTGCTGAACAGCAAACACCGCTTATCATTATTTTTATTTTTATTGATGAACGCGGC
CTCTTCCGTATTTACATTATTAGTCCCGTTTCAAAATAAACCCGCTTTTACCCTGCCTTTATTAGGTATACCGCTATTTT
GTCTGGCGGCATTTTTATTTACCTTGCAGTCTCCGCGGTTATACGTCAAAAAACTGAATCTATTTTCCTGCGTCCTGGGT
CTGCTGTGGGCGGCACATATTTACCTTAAAAGTCAGCACTGTGCGCCGAACAACCAAAGTTTTCTATTAATTAGCCTGTT
CAGTATGTTTTTTATCAGCGCCATTGCCTTAACCGATAATTTTATCGCCTTCTGCCTGCACGCCGTTCCATCCGCATTAG
TCATTCTATTACTGGACGGTATGCACAATACCATGCGGATAGTATTCACCACGGTATTGCCGATTATCGCGTTTTCTATC
CATCACCTGATGCTTAAACGCAGCGAACACTTTACCCAAACCCTGGTGGCTAATCTGTATAACGAACGGGACAGGTTCAG
CAATTTAAGCATGATCGATCCCTTAACCGGGCTCTATAACCGTCGTGGCCTGGAGAATAAAATCAATATGTTGCCGGCGG
CCGCGTCAGACTGCCATTTTGTACTGCTGCTCGATATCGATCACTTCAAGGCTTATAACGATAATTATGGCCATACCATG
GGTGACCAGGCGCTGGTGCAGGTCGCCATCGCCATCCGCGATTCGGTACGTTCACGCGATATCGTGGTGCGTTACGGTGG
AGAGGAGTTTTTGGTGCTGTTGACCAATGTGAATGAGGACTATGCCACCCAAGTGGCTGAACGGGTGCGCCAGCGGGTGC
AGGAACTCAAAATCCCCCATCGCTTTAACCAGTTTGAAACCACCACGGTCACGCTCAGTGCCGGGATCTCCGCATTGGAC
AAGCTGGATGTGGCCTCGGCGATCCGGGCGGCCGACGCCGCGTTATATCAGGCGAAAAACAACGGCCGCAATAATATTCA
GCTGGCGCAAAACGTTCAACCCGCCGCCGTGTGA

Upstream 100 bases:

>100_bases
CAATCTACAACCATACAATGCGAGTGTTTAATTCCCACCGACAGAATTTTTCGCAACCTTACCTTTTCGACAGATCGATA
TTGTAAAGAGTGAATTCCGC

Downstream 100 bases:

>100_bases
GCCGGCGGGCTGTGAGCTTAGCGGCGGCGCTGCTGCAGGCCGTAAGCAAACACCACGGCGAAGCACAGCAGCGGCAGGCA
ATACGACAACGCCGTGCTAT

Product: diguanylate cyclase

Products: NA

Alternate protein names: DGC [H]

Number of amino acids: Translated: 357; Mature: 357

Protein sequence:

>357_residues
MNARSYQELLNSKHRLSLFLFLLMNAASSVFTLLVPFQNKPAFTLPLLGIPLFCLAAFLFTLQSPRLYVKKLNLFSCVLG
LLWAAHIYLKSQHCAPNNQSFLLISLFSMFFISAIALTDNFIAFCLHAVPSALVILLLDGMHNTMRIVFTTVLPIIAFSI
HHLMLKRSEHFTQTLVANLYNERDRFSNLSMIDPLTGLYNRRGLENKINMLPAAASDCHFVLLLDIDHFKAYNDNYGHTM
GDQALVQVAIAIRDSVRSRDIVVRYGGEEFLVLLTNVNEDYATQVAERVRQRVQELKIPHRFNQFETTTVTLSAGISALD
KLDVASAIRAADAALYQAKNNGRNNIQLAQNVQPAAV

Sequences:

>Translated_357_residues
MNARSYQELLNSKHRLSLFLFLLMNAASSVFTLLVPFQNKPAFTLPLLGIPLFCLAAFLFTLQSPRLYVKKLNLFSCVLG
LLWAAHIYLKSQHCAPNNQSFLLISLFSMFFISAIALTDNFIAFCLHAVPSALVILLLDGMHNTMRIVFTTVLPIIAFSI
HHLMLKRSEHFTQTLVANLYNERDRFSNLSMIDPLTGLYNRRGLENKINMLPAAASDCHFVLLLDIDHFKAYNDNYGHTM
GDQALVQVAIAIRDSVRSRDIVVRYGGEEFLVLLTNVNEDYATQVAERVRQRVQELKIPHRFNQFETTTVTLSAGISALD
KLDVASAIRAADAALYQAKNNGRNNIQLAQNVQPAAV
>Mature_357_residues
MNARSYQELLNSKHRLSLFLFLLMNAASSVFTLLVPFQNKPAFTLPLLGIPLFCLAAFLFTLQSPRLYVKKLNLFSCVLG
LLWAAHIYLKSQHCAPNNQSFLLISLFSMFFISAIALTDNFIAFCLHAVPSALVILLLDGMHNTMRIVFTTVLPIIAFSI
HHLMLKRSEHFTQTLVANLYNERDRFSNLSMIDPLTGLYNRRGLENKINMLPAAASDCHFVLLLDIDHFKAYNDNYGHTM
GDQALVQVAIAIRDSVRSRDIVVRYGGEEFLVLLTNVNEDYATQVAERVRQRVQELKIPHRFNQFETTTVTLSAGISALD
KLDVASAIRAADAALYQAKNNGRNNIQLAQNVQPAAV

Specific function: A probable diguanylate cyclase, overexpression leads to a strong repression of swimming; swimming returns to normal when residues 359-360 are both mutated to Ala. Overexpression also leads to a 20-fold increase in c-di-GMP levels in vivo. Cyclic-di- GMP i

COG id: COG2199

COG function: function code T; FOG: GGDEF domain

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GGDEF domain [H]

Homologues:

Organism=Escherichia coli, GI1787262, Length=168, Percent_Identity=36.9047619047619, Blast_Score=118, Evalue=7e-28,
Organism=Escherichia coli, GI1786584, Length=240, Percent_Identity=31.25, Blast_Score=110, Evalue=2e-25,
Organism=Escherichia coli, GI145693134, Length=181, Percent_Identity=38.121546961326, Blast_Score=107, Evalue=1e-24,
Organism=Escherichia coli, GI1788085, Length=176, Percent_Identity=34.6590909090909, Blast_Score=105, Evalue=4e-24,
Organism=Escherichia coli, GI87082007, Length=197, Percent_Identity=35.0253807106599, Blast_Score=92, Evalue=3e-20,
Organism=Escherichia coli, GI1787541, Length=164, Percent_Identity=34.7560975609756, Blast_Score=91, Evalue=9e-20,
Organism=Escherichia coli, GI1787816, Length=159, Percent_Identity=37.1069182389937, Blast_Score=89, Evalue=3e-19,
Organism=Escherichia coli, GI87081881, Length=157, Percent_Identity=35.6687898089172, Blast_Score=89, Evalue=4e-19,
Organism=Escherichia coli, GI1787802, Length=158, Percent_Identity=37.3417721518987, Blast_Score=89, Evalue=4e-19,
Organism=Escherichia coli, GI87081974, Length=154, Percent_Identity=34.4155844155844, Blast_Score=89, Evalue=5e-19,
Organism=Escherichia coli, GI1788381, Length=169, Percent_Identity=33.1360946745562, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI87081977, Length=159, Percent_Identity=33.9622641509434, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI1788956, Length=151, Percent_Identity=33.112582781457, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1787056, Length=187, Percent_Identity=26.2032085561497, Blast_Score=68, Evalue=9e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160 [H]

Pfam domain/function: PF00990 GGDEF [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 40132; Mature: 40132

Theoretical pI: Translated: 8.91; Mature: 8.91

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNARSYQELLNSKHRLSLFLFLLMNAASSVFTLLVPFQNKPAFTLPLLGIPLFCLAAFLF
CCCHHHHHHHCCHHHHHHHHHHHHHHHHHHEEEEEECCCCCCEECHHHHHHHHHHHHHHH
TLQSPRLYVKKLNLFSCVLGLLWAAHIYLKSQHCAPNNQSFLLISLFSMFFISAIALTDN
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
FIAFCLHAVPSALVILLLDGMHNTMRIVFTTVLPIIAFSIHHLMLKRSEHFTQTLVANLY
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NERDRFSNLSMIDPLTGLYNRRGLENKINMLPAAASDCHFVLLLDIDHFKAYNDNYGHTM
HHHHHHCCCCHHHHHHHHHHCCCCCHHHHHCCCCCCCCEEEEEEEEHHHHCCCCCCCCCC
GDQALVQVAIAIRDSVRSRDIVVRYGGEEFLVLLTNVNEDYATQVAERVRQRVQELKIPH
CHHHHHHHHHHHHHCCCCCCEEEEECCCEEEEEEECCCHHHHHHHHHHHHHHHHHCCCCC
RFNQFETTTVTLSAGISALDKLDVASAIRAADAALYQAKNNGRNNIQLAQNVQPAAV
CCCCCCEEEEEEHHCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCC
>Mature Secondary Structure
MNARSYQELLNSKHRLSLFLFLLMNAASSVFTLLVPFQNKPAFTLPLLGIPLFCLAAFLF
CCCHHHHHHHCCHHHHHHHHHHHHHHHHHHEEEEEECCCCCCEECHHHHHHHHHHHHHHH
TLQSPRLYVKKLNLFSCVLGLLWAAHIYLKSQHCAPNNQSFLLISLFSMFFISAIALTDN
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
FIAFCLHAVPSALVILLLDGMHNTMRIVFTTVLPIIAFSIHHLMLKRSEHFTQTLVANLY
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NERDRFSNLSMIDPLTGLYNRRGLENKINMLPAAASDCHFVLLLDIDHFKAYNDNYGHTM
HHHHHHCCCCHHHHHHHHHHCCCCCHHHHHCCCCCCCCEEEEEEEEHHHHCCCCCCCCCC
GDQALVQVAIAIRDSVRSRDIVVRYGGEEFLVLLTNVNEDYATQVAERVRQRVQELKIPH
CHHHHHHHHHHHHHCCCCCCEEEEECCCEEEEEEECCCHHHHHHHHHHHHHHHHHCCCCC
RFNQFETTTVTLSAGISALDKLDVASAIRAADAALYQAKNNGRNNIQLAQNVQPAAV
CCCCCCEEEEEEHHCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503 [H]