Click here to switch to the map view.

The map label for this gene is ascF [H]

Identifier: 157368821

GI number: 157368821

Start: 639807

End: 641249

Strand: Reverse

Name: ascF [H]

Synonym: Spro_0576

Alternate gene names: 157368821

Gene position: 641249-639807 (Counterclockwise)

Preceding gene: 157368823

Following gene: 157368820

Centisome position: 11.77

GC content: 60.15

Gene sequence:

>1443_bases
ATGCCGAAGAATTACGCAGAGATCTCACCCGCTATCGTGAAGGCGCTGGGTGGCCTCACCAATATTGAAGCCGTGACCCA
CTGCATGACCCGCCTGCGGTTCGTGGTGAAAGACAAGGCGCTGATCGACACCCTGGCGCTGAAAGCCATTAATGGCGTAT
TGGGCGTGGTGCACAGCGGTGAGCAATGCCAGGTGATCATCGGCAATGAAGTGTCGAAGGCTTATCAGGCGGTGCTGGCG
CTCGGCATGCCGGGTAACCCCCCCACCGCCACTCCGGCAAAACGCAAAATCACCCTGAAAGGCATTGGTGCCGGCATTCT
CGACGCCCTGATCGGCACCATGTCGCCGTTGATCCCGGCCATCATCGGCGGTTCGATGGTGAAACTGCTGGCGATGATCC
TGGATATGTTCGGCGTATTCGAAAAAGGCTCGTCGACCCTGGTGATACTGAACGTGATTGGCGACGGCGCGTTCTTCTTC
CTGCCGGTGATGGTGGCCGCCTCTGCGGCGCTGAAATTCAAAACCAACATGTCACTGGCAATCGCCATTGCCGGGGTGCT
GCTGCACCCCAACTTTATCGACCTGATGGCCAAGGCGGCGCAGGGCCAGGCGGTGGAGTTCGCCTACATCCCGGTGACGG
CGGTGAAATACACCTACACGGTGATCCCCGCGCTGGTGATGACCTGGATATTGTCGTACATCGAGCGCTGGGTCGATCGC
ATCACCCCGGCGGTGACCAAGAACTTCCTTAAGCCGATGCTGATTGTGCTGATTGCCGCGCCGATCGCGATAGTGTTTAT
CGGCCCAGTCGGCATCTGGATCGGCAGCGGGATTTCGGCGCTGGTGTACACCGTGCATGGTTATCTGGGCTGGCTTTCCG
TCGCCATTATGGGGGCCATTTGGCCGCTGCTGGTGATGACCGGCATGCACCGCGTATTCACGCCAACCATCATTCAGACC
ATCGCCGAAACCGGCAAAGAGGGCATGGTGATGCCTTCGGAGATTGGTGCCAACCTGTCGCTCGGCGGCTCTTCGCTGGC
GGTGGCGTTCAAAACCAAGAACCGCGAACTGCGCCAGACCGCCTTGGCCGCTGCCGCTTCCGCCATTGTCGCGGGCATTT
CCGAACCGGCACTGTACGGCGTGGCAGTGCGGCTCAAACGCCCGTTGATCGCCAGTTTGATCAGCGGTTTTATCTGCGGT
GCCGTCGCCGGTCTCGGCGGGTTGGCCAGCCATTCGATGGCTTCGCCCGGCCTGTTCACCAGCGTGCAGTTCTTCGACCC
GACCAACCCGATGAGCATAGTCTGGGTCGGCGGCGTGATGGTGCTTGCCGTGGTGCTGTCATTCGTGCTCACGCTGCTGT
TGGGCTTTGAAGACATTCCGGAAAACGAAGAGGCCCCAACGACAACCGCGCCTGCGGCCAATACCGCTAACGCTACCCAC
TGA

Upstream 100 bases:

>100_bases
TTCTTATGGGCGTTTCATTCAGATGGAACGGCGGATCGCCCAGGCAATAAAAGTTCGCCTTTGGGCAATCCGAATTCCTA
TCTAAAGAGACGATAATTCC

Downstream 100 bases:

>100_bases
GACAATTGAGGATTGCATGTCTGATTCAACTTTCCCGCAAGGGTTTTTATGGGGCGGCGCCCTGGCCGCCAACCAGTCTG
AAGGGGGTTATCGCGAGGGC

Product: PTS system cellobiose/arbutin/salicin-specific transporter subunits IIBC

Products: NA

Alternate protein names: EIIBC-Asc; EII-Asc; Arbutin-, cellobiose-, and salicin-specific phosphotransferase enzyme IIB component; PTS system arbutin-, cellobiose-, and salicin-specific EIIB component; Arbutin, cellobiose, and salicin permease IIC component; PTS system arbutin-, cellobiose-, and salicin-specific EIIC component [H]

Number of amino acids: Translated: 480; Mature: 479

Protein sequence:

>480_residues
MPKNYAEISPAIVKALGGLTNIEAVTHCMTRLRFVVKDKALIDTLALKAINGVLGVVHSGEQCQVIIGNEVSKAYQAVLA
LGMPGNPPTATPAKRKITLKGIGAGILDALIGTMSPLIPAIIGGSMVKLLAMILDMFGVFEKGSSTLVILNVIGDGAFFF
LPVMVAASAALKFKTNMSLAIAIAGVLLHPNFIDLMAKAAQGQAVEFAYIPVTAVKYTYTVIPALVMTWILSYIERWVDR
ITPAVTKNFLKPMLIVLIAAPIAIVFIGPVGIWIGSGISALVYTVHGYLGWLSVAIMGAIWPLLVMTGMHRVFTPTIIQT
IAETGKEGMVMPSEIGANLSLGGSSLAVAFKTKNRELRQTALAAAASAIVAGISEPALYGVAVRLKRPLIASLISGFICG
AVAGLGGLASHSMASPGLFTSVQFFDPTNPMSIVWVGGVMVLAVVLSFVLTLLLGFEDIPENEEAPTTTAPAANTANATH

Sequences:

>Translated_480_residues
MPKNYAEISPAIVKALGGLTNIEAVTHCMTRLRFVVKDKALIDTLALKAINGVLGVVHSGEQCQVIIGNEVSKAYQAVLA
LGMPGNPPTATPAKRKITLKGIGAGILDALIGTMSPLIPAIIGGSMVKLLAMILDMFGVFEKGSSTLVILNVIGDGAFFF
LPVMVAASAALKFKTNMSLAIAIAGVLLHPNFIDLMAKAAQGQAVEFAYIPVTAVKYTYTVIPALVMTWILSYIERWVDR
ITPAVTKNFLKPMLIVLIAAPIAIVFIGPVGIWIGSGISALVYTVHGYLGWLSVAIMGAIWPLLVMTGMHRVFTPTIIQT
IAETGKEGMVMPSEIGANLSLGGSSLAVAFKTKNRELRQTALAAAASAIVAGISEPALYGVAVRLKRPLIASLISGFICG
AVAGLGGLASHSMASPGLFTSVQFFDPTNPMSIVWVGGVMVLAVVLSFVLTLLLGFEDIPENEEAPTTTAPAANTANATH
>Mature_479_residues
PKNYAEISPAIVKALGGLTNIEAVTHCMTRLRFVVKDKALIDTLALKAINGVLGVVHSGEQCQVIIGNEVSKAYQAVLAL
GMPGNPPTATPAKRKITLKGIGAGILDALIGTMSPLIPAIIGGSMVKLLAMILDMFGVFEKGSSTLVILNVIGDGAFFFL
PVMVAASAALKFKTNMSLAIAIAGVLLHPNFIDLMAKAAQGQAVEFAYIPVTAVKYTYTVIPALVMTWILSYIERWVDRI
TPAVTKNFLKPMLIVLIAAPIAIVFIGPVGIWIGSGISALVYTVHGYLGWLSVAIMGAIWPLLVMTGMHRVFTPTIIQTI
AETGKEGMVMPSEIGANLSLGGSSLAVAFKTKNRELRQTALAAAASAIVAGISEPALYGVAVRLKRPLIASLISGFICGA
VAGLGGLASHSMASPGLFTSVQFFDPTNPMSIVWVGGVMVLAVVLSFVLTLLLGFEDIPENEEAPTTTAPAANTANATH

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This

COG id: COG1263

COG function: function code G; Phosphotransferase system IIC components, glucose/maltose/N-acetylglucosamine-specific

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIC type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI48994906, Length=466, Percent_Identity=83.0472103004292, Blast_Score=744, Evalue=0.0,
Organism=Escherichia coli, GI1790159, Length=486, Percent_Identity=31.0699588477366, Blast_Score=195, Evalue=5e-51,
Organism=Escherichia coli, GI2367362, Length=428, Percent_Identity=26.8691588785047, Blast_Score=131, Evalue=8e-32,
Organism=Escherichia coli, GI1788769, Length=480, Percent_Identity=26.0416666666667, Blast_Score=95, Evalue=1e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018113
- InterPro:   IPR004719
- InterPro:   IPR001996
- InterPro:   IPR003352
- InterPro:   IPR013013
- InterPro:   IPR011535 [H]

Pfam domain/function: PF00367 PTS_EIIB; PF02378 PTS_EIIC [H]

EC number: =2.7.1.69 [H]

Molecular weight: Translated: 50233; Mature: 50101

Theoretical pI: Translated: 9.43; Mature: 9.43

Prosite motif: PS01035 PTS_EIIB_TYPE_1_CYS ; PS51098 PTS_EIIB_TYPE_1 ; PS51103 PTS_EIIC_TYPE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPKNYAEISPAIVKALGGLTNIEAVTHCMTRLRFVVKDKALIDTLALKAINGVLGVVHSG
CCCCHHHCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
EQCQVIIGNEVSKAYQAVLALGMPGNPPTATPAKRKITLKGIGAGILDALIGTMSPLIPA
CCEEEEECCHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHH
IIGGSMVKLLAMILDMFGVFEKGSSTLVILNVIGDGAFFFLPVMVAASAALKFKTNMSLA
HHCHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCHHHHHHHHHHHHHHHEEECCCHHH
IAIAGVLLHPNFIDLMAKAAQGQAVEFAYIPVTAVKYTYTVIPALVMTWILSYIERWVDR
HHHHHHHHCCHHHHHHHHHCCCCEEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ITPAVTKNFLKPMLIVLIAAPIAIVFIGPVGIWIGSGISALVYTVHGYLGWLSVAIMGAI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
WPLLVMTGMHRVFTPTIIQTIAETGKEGMVMPSEIGANLSLGGSSLAVAFKTKNRELRQT
HHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCHHHHCCCEEECCCEEEEEEECCCHHHHHH
ALAAAASAIVAGISEPALYGVAVRLKRPLIASLISGFICGAVAGLGGLASHSMASPGLFT
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEE
SVQFFDPTNPMSIVWVGGVMVLAVVLSFVLTLLLGFEDIPENEEAPTTTAPAANTANATH
EEEEECCCCCCEEEEHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
PKNYAEISPAIVKALGGLTNIEAVTHCMTRLRFVVKDKALIDTLALKAINGVLGVVHSG
CCCHHHCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
EQCQVIIGNEVSKAYQAVLALGMPGNPPTATPAKRKITLKGIGAGILDALIGTMSPLIPA
CCEEEEECCHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHH
IIGGSMVKLLAMILDMFGVFEKGSSTLVILNVIGDGAFFFLPVMVAASAALKFKTNMSLA
HHCHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCHHHHHHHHHHHHHHHEEECCCHHH
IAIAGVLLHPNFIDLMAKAAQGQAVEFAYIPVTAVKYTYTVIPALVMTWILSYIERWVDR
HHHHHHHHCCHHHHHHHHHCCCCEEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ITPAVTKNFLKPMLIVLIAAPIAIVFIGPVGIWIGSGISALVYTVHGYLGWLSVAIMGAI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
WPLLVMTGMHRVFTPTIIQTIAETGKEGMVMPSEIGANLSLGGSSLAVAFKTKNRELRQT
HHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCHHHHCCCEEECCCEEEEEEECCCHHHHHH
ALAAAASAIVAGISEPALYGVAVRLKRPLIASLISGFICGAVAGLGGLASHSMASPGLFT
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEE
SVQFFDPTNPMSIVWVGGVMVLAVVLSFVLTLLLGFEDIPENEEAPTTTAPAANTANATH
EEEEECCCCCCEEEEHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1630307; 9278503 [H]