Click here to switch to the map view.

The map label for this gene is ascB [H]

Identifier: 157368820

GI number: 157368820

Start: 638360

End: 639790

Strand: Reverse

Name: ascB [H]

Synonym: Spro_0575

Alternate gene names: 157368820

Gene position: 639790-638360 (Counterclockwise)

Preceding gene: 157368821

Following gene: 157368819

Centisome position: 11.74

GC content: 60.03

Gene sequence:

>1431_bases
ATGTCTGATTCAACTTTCCCGCAAGGGTTTTTATGGGGCGGCGCCCTGGCCGCCAACCAGTCTGAAGGGGGTTATCGCGA
GGGCGGCAAAGGGCTGACCACGGTTGACATGATCCCGCATGGCCCCAACCGCATGCCGGTAAAGCTCGGCCTGGAGAAGC
GTTTTGCCCTGCGCGAAGACGAGTTTTACCCCAGCCACGACGCGATTGATTTTTACCACCGCTACCGCGACGACATTGCG
CTGATGGCGGAGATGGGCTTTAGCGTATTCCGCACCTCCATTGCCTGGAGCCGGATTTACCCGAACGGCGACGAGCTGAC
GCCCAACCCGGAAGGCATCGCCTTCTACCGCAGCCTGTTCAAAGAGTGCCGTAAATACGGCATCGAGCCGCTGGTGACGC
TGTGCCATTTCGACGTGCCAATGCACCTGGTGACCGAATACGGCTCCTGGCGCAGCCGCAAGATGGTGGAGTTTTTCACC
CGCTATGCCCGCACCTGCTTCGAAGCCTTCGACGGATTGGTGAAGTATTGGCTCACCTTCAACGAGATCAACATTGTGCT
GCACAGCCCGTTCTCCGGCGCAGGGTTGGTGTTTGAGGCGGACGAGAACCCCGAACAGGTAAAATACCAGGCCGTGCACC
ACGAACTAATTGCCAGCGCACTGGCGACCCGCATCGCGCACGAGGTGAACCCCGCCAACCAGGTGGGCTGCATGCTGGCG
GGCGGCAATTTTTACCCCTGGTCCAGCAAGCCGGAAGACGTGTGGGCCGCGCTGGAGAAAGACCGCGAGAACCTGTTCTT
TATCGACGTGCAGGCCCGCGGTGCCTACCCTTCTTATTCTGCCCGGGTATTCCGCGAGAAAGGCGTGAGCATCGCGATGG
AGGAAGGCGACGCCGATATCCTGAAGAACACCGTCGATTTTGTCTCCTTCAGCTATTACGCCTCGCGCTGCGCGTCTGCC
GATATGAACGACAACAACAGCAGCGCCGCCAACGTGGTGAAATCCCTGACCAACCCGCATATTCCGCGCAGTGACTGGGG
CTGGGGCATCGATCCGTTGGGTTTGCGCATCACCATGAACATGATGTACGACCGCTACCAAAAACCGCTATTCCTGGTGG
AAAACGGCCTGGGCGCGCACGACGAGTTCAATGCCGACGGCGAGATCGCCGACGATTACCGCATCAGCTACCTGCGGGAG
CATATCCGCGCCATGGCCGACGCCATCGCCGACGGCGTGCCGGTGATGGGCTACACCACCTGGGGCTGCATTGACCTGGT
GGCGGCATCGACCGGGGAAATGAGCAAGCGCTACGGCCTGGTTTACGTCGACCGTGACGACCACGGCAACGGCACCCTCA
CCCGCACGCGCAAGAAATCTTTCTGGTGGTACAAGCAGGTGATTGCCAGTAATGGGGCGGATTTGGGGTGA

Upstream 100 bases:

>100_bases
TTGGGCTTTGAAGACATTCCGGAAAACGAAGAGGCCCCAACGACAACCGCGCCTGCGGCCAATACCGCTAACGCTACCCA
CTGAGACAATTGAGGATTGC

Downstream 100 bases:

>100_bases
TGGGGTTGGGGGGGTGAGTACCGATGCTGGTGATAGTTGCGAGCTGGATTCCAGACTGAGTAGTAAGCCCCTGTAATTCT
ATCCAGGTTGTTTTTTAGCC

Product: cryptic 6-phospho-beta-glucosidase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 476; Mature: 475

Protein sequence:

>476_residues
MSDSTFPQGFLWGGALAANQSEGGYREGGKGLTTVDMIPHGPNRMPVKLGLEKRFALREDEFYPSHDAIDFYHRYRDDIA
LMAEMGFSVFRTSIAWSRIYPNGDELTPNPEGIAFYRSLFKECRKYGIEPLVTLCHFDVPMHLVTEYGSWRSRKMVEFFT
RYARTCFEAFDGLVKYWLTFNEINIVLHSPFSGAGLVFEADENPEQVKYQAVHHELIASALATRIAHEVNPANQVGCMLA
GGNFYPWSSKPEDVWAALEKDRENLFFIDVQARGAYPSYSARVFREKGVSIAMEEGDADILKNTVDFVSFSYYASRCASA
DMNDNNSSAANVVKSLTNPHIPRSDWGWGIDPLGLRITMNMMYDRYQKPLFLVENGLGAHDEFNADGEIADDYRISYLRE
HIRAMADAIADGVPVMGYTTWGCIDLVAASTGEMSKRYGLVYVDRDDHGNGTLTRTRKKSFWWYKQVIASNGADLG

Sequences:

>Translated_476_residues
MSDSTFPQGFLWGGALAANQSEGGYREGGKGLTTVDMIPHGPNRMPVKLGLEKRFALREDEFYPSHDAIDFYHRYRDDIA
LMAEMGFSVFRTSIAWSRIYPNGDELTPNPEGIAFYRSLFKECRKYGIEPLVTLCHFDVPMHLVTEYGSWRSRKMVEFFT
RYARTCFEAFDGLVKYWLTFNEINIVLHSPFSGAGLVFEADENPEQVKYQAVHHELIASALATRIAHEVNPANQVGCMLA
GGNFYPWSSKPEDVWAALEKDRENLFFIDVQARGAYPSYSARVFREKGVSIAMEEGDADILKNTVDFVSFSYYASRCASA
DMNDNNSSAANVVKSLTNPHIPRSDWGWGIDPLGLRITMNMMYDRYQKPLFLVENGLGAHDEFNADGEIADDYRISYLRE
HIRAMADAIADGVPVMGYTTWGCIDLVAASTGEMSKRYGLVYVDRDDHGNGTLTRTRKKSFWWYKQVIASNGADLG
>Mature_475_residues
SDSTFPQGFLWGGALAANQSEGGYREGGKGLTTVDMIPHGPNRMPVKLGLEKRFALREDEFYPSHDAIDFYHRYRDDIAL
MAEMGFSVFRTSIAWSRIYPNGDELTPNPEGIAFYRSLFKECRKYGIEPLVTLCHFDVPMHLVTEYGSWRSRKMVEFFTR
YARTCFEAFDGLVKYWLTFNEINIVLHSPFSGAGLVFEADENPEQVKYQAVHHELIASALATRIAHEVNPANQVGCMLAG
GNFYPWSSKPEDVWAALEKDRENLFFIDVQARGAYPSYSARVFREKGVSIAMEEGDADILKNTVDFVSFSYYASRCASAD
MNDNNSSAANVVKSLTNPHIPRSDWGWGIDPLGLRITMNMMYDRYQKPLFLVENGLGAHDEFNADGEIADDYRISYLREH
IRAMADAIADGVPVMGYTTWGCIDLVAASTGEMSKRYGLVYVDRDDHGNGTLTRTRKKSFWWYKQVIASNGADLG

Specific function: Can hydrolyze salicin, cellobiose, and probably arbutin [H]

COG id: COG2723

COG function: function code G; Beta-glucosidase/6-phospho-beta-glucosidase/beta-galactosidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 1 family [H]

Homologues:

Organism=Homo sapiens, GI110681710, Length=503, Percent_Identity=30.0198807157058, Blast_Score=178, Evalue=1e-44,
Organism=Homo sapiens, GI32481206, Length=495, Percent_Identity=29.4949494949495, Blast_Score=170, Evalue=2e-42,
Organism=Homo sapiens, GI13273313, Length=493, Percent_Identity=26.9776876267748, Blast_Score=156, Evalue=3e-38,
Organism=Homo sapiens, GI24497614, Length=487, Percent_Identity=28.1314168377823, Blast_Score=142, Evalue=5e-34,
Organism=Homo sapiens, GI28376633, Length=483, Percent_Identity=24.8447204968944, Blast_Score=130, Evalue=2e-30,
Organism=Escherichia coli, GI1789070, Length=472, Percent_Identity=85.1694915254237, Blast_Score=863, Evalue=0.0,
Organism=Escherichia coli, GI2367270, Length=470, Percent_Identity=53.6170212765957, Blast_Score=528, Evalue=1e-151,
Organism=Escherichia coli, GI2367174, Length=478, Percent_Identity=50.2092050209205, Blast_Score=498, Evalue=1e-142,
Organism=Caenorhabditis elegans, GI17552856, Length=488, Percent_Identity=31.3524590163934, Blast_Score=182, Evalue=3e-46,
Organism=Caenorhabditis elegans, GI17539390, Length=496, Percent_Identity=30.241935483871, Blast_Score=170, Evalue=2e-42,
Organism=Drosophila melanogaster, GI21356577, Length=499, Percent_Identity=25.0501002004008, Blast_Score=132, Evalue=4e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001360
- InterPro:   IPR018120
- InterPro:   IPR017853
- InterPro:   IPR013781 [H]

Pfam domain/function: PF00232 Glyco_hydro_1 [H]

EC number: =3.2.1.86 [H]

Molecular weight: Translated: 53764; Mature: 53633

Theoretical pI: Translated: 5.34; Mature: 5.34

Prosite motif: PS00572 GLYCOSYL_HYDROL_F1_1 ; PS00653 GLYCOSYL_HYDROL_F1_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDSTFPQGFLWGGALAANQSEGGYREGGKGLTTVDMIPHGPNRMPVKLGLEKRFALRED
CCCCCCCCCEEECCEEECCCCCCCCCCCCCCCEEEEECCCCCCCCCEEECCHHHHHCCCC
EFYPSHDAIDFYHRYRDDIALMAEMGFSVFRTSIAWSRIYPNGDELTPNPEGIAFYRSLF
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHH
KECRKYGIEPLVTLCHFDVPMHLVTEYGSWRSRKMVEFFTRYARTCFEAFDGLVKYWLTF
HHHHHCCCHHHHHHHHCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHEEE
NEINIVLHSPFSGAGLVFEADENPEQVKYQAVHHELIASALATRIAHEVNPANQVGCMLA
EEEEEEEECCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEE
GGNFYPWSSKPEDVWAALEKDRENLFFIDVQARGAYPSYSARVFREKGVSIAMEEGDADI
CCEECCCCCCHHHHHHHHHCCCCCEEEEEEEECCCCCCHHHHHHHHCCCEEEEECCCHHH
LKNTVDFVSFSYYASRCASADMNDNNSSAANVVKSLTNPHIPRSDWGWGIDPLGLRITMN
HHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEHH
MMYDRYQKPLFLVENGLGAHDEFNADGEIADDYRISYLREHIRAMADAIADGVPVMGYTT
HHHHHHCCCEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCH
WGCIDLVAASTGEMSKRYGLVYVDRDDHGNGTLTRTRKKSFWWYKQVIASNGADLG
HHHHHHHHCCCCCHHHHCCEEEEECCCCCCCCEEECHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
SDSTFPQGFLWGGALAANQSEGGYREGGKGLTTVDMIPHGPNRMPVKLGLEKRFALRED
CCCCCCCCEEECCEEECCCCCCCCCCCCCCCEEEEECCCCCCCCCEEECCHHHHHCCCC
EFYPSHDAIDFYHRYRDDIALMAEMGFSVFRTSIAWSRIYPNGDELTPNPEGIAFYRSLF
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHH
KECRKYGIEPLVTLCHFDVPMHLVTEYGSWRSRKMVEFFTRYARTCFEAFDGLVKYWLTF
HHHHHCCCHHHHHHHHCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHEEE
NEINIVLHSPFSGAGLVFEADENPEQVKYQAVHHELIASALATRIAHEVNPANQVGCMLA
EEEEEEEECCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEE
GGNFYPWSSKPEDVWAALEKDRENLFFIDVQARGAYPSYSARVFREKGVSIAMEEGDADI
CCEECCCCCCHHHHHHHHHCCCCCEEEEEEEECCCCCCHHHHHHHHCCCEEEEECCCHHH
LKNTVDFVSFSYYASRCASADMNDNNSSAANVVKSLTNPHIPRSDWGWGIDPLGLRITMN
HHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEHH
MMYDRYQKPLFLVENGLGAHDEFNADGEIADDYRISYLREHIRAMADAIADGVPVMGYTT
HHHHHHCCCEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCH
WGCIDLVAASTGEMSKRYGLVYVDRDDHGNGTLTRTRKKSFWWYKQVIASNGADLG
HHHHHHHHCCCCCHHHHCCEEEEECCCCCCCCEEECHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1630307; 9278503 [H]