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The map label for this gene is vsr [H]

Identifier: 157368817

GI number: 157368817

Start: 635584

End: 636057

Strand: Reverse

Name: vsr [H]

Synonym: Spro_0572

Alternate gene names: 157368817

Gene position: 636057-635584 (Counterclockwise)

Preceding gene: 157368818

Following gene: 157368816

Centisome position: 11.67

GC content: 43.46

Gene sequence:

>474_bases
ATGGCTGACGTACACTCCCCCTCAATCAGAAGCAAGAACATGAGGGCGATACGTAATCAAAATACAGCCATTGAGAAATT
GTTATCCCGGGTTCTGGATGAAGCTGGGATAACTTACCGTACCCAAGCTTCTGATCTCCCTGGTAAACCTGACTTCGTTA
TTGATGATTATAAAGCCATAATTTTCGTCCACGGCTGCTTCTGGCATCATCATGATTGTTACCTGTTCAAAGTGCCCGCG
ACAAGGACCGAGTTTTGGTTAAAAAAGATCGATTCAAATGTCAGGCGGGATAATGACGTCACCCGACTACTGACAGAACA
AGGCTGGCGTGAATTGATCATCTGGGAGTGTTCTCTGAAAGGCCGAATAAAGCTTTCAGCTAATGCATTATCAGAAAGGA
TTGAGGAGTGGCTATGCGCAAGTGATACTCATGCTGTGATCGATTCTAAAGGTATTCATCAATTAAATAATTAG

Upstream 100 bases:

>100_bases
CAGTCATTGTGCCGGCATTTGCAGCCGTAGCTAAGTTACTAGAACCTTACATTGCTTCGGCTGTTTCAAAAGACAAAAGT
GAAGCCGCATAAGGTAAATT

Downstream 100 bases:

>100_bases
CATCGAAATAATTCCATTAAGTAATCCTCCAGTACCAAGAAAAAACCTCGCAATGCACTAAAACTTTGTCTCTCATCATC
GACATCTATTCAATAACGTG

Product: DNA mismatch endonuclease vsr

Products: DNA [C]

Alternate protein names: DNA mismatch endonuclease; V.EcoKDcm; Vsr mismatch endonuclease [H]

Number of amino acids: Translated: 157; Mature: 156

Protein sequence:

>157_residues
MADVHSPSIRSKNMRAIRNQNTAIEKLLSRVLDEAGITYRTQASDLPGKPDFVIDDYKAIIFVHGCFWHHHDCYLFKVPA
TRTEFWLKKIDSNVRRDNDVTRLLTEQGWRELIIWECSLKGRIKLSANALSERIEEWLCASDTHAVIDSKGIHQLNN

Sequences:

>Translated_157_residues
MADVHSPSIRSKNMRAIRNQNTAIEKLLSRVLDEAGITYRTQASDLPGKPDFVIDDYKAIIFVHGCFWHHHDCYLFKVPA
TRTEFWLKKIDSNVRRDNDVTRLLTEQGWRELIIWECSLKGRIKLSANALSERIEEWLCASDTHAVIDSKGIHQLNN
>Mature_156_residues
ADVHSPSIRSKNMRAIRNQNTAIEKLLSRVLDEAGITYRTQASDLPGKPDFVIDDYKAIIFVHGCFWHHHDCYLFKVPAT
RTEFWLKKIDSNVRRDNDVTRLLTEQGWRELIIWECSLKGRIKLSANALSERIEEWLCASDTHAVIDSKGIHQLNN

Specific function: Deamination of 5-methylcytosine in DNA results in T/G mismatches. If unrepaired, these mismatches can lead to C-to-C transition mutations. The very short patch (VSP) repair process in E.coli counteracts the mutagenic process by repairing the mismatches in

COG id: COG3727

COG function: function code L; DNA G:T-mismatch repair endonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the vsr family [H]

Homologues:

Organism=Escherichia coli, GI1788270, Length=156, Percent_Identity=60.8974358974359, Blast_Score=208, Evalue=1e-55,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004603
- InterPro:   IPR011335 [H]

Pfam domain/function: PF03852 Vsr [H]

EC number: 3.1.-.- [C]

Molecular weight: Translated: 18175; Mature: 18044

Theoretical pI: Translated: 8.01; Mature: 8.01

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADVHSPSIRSKNMRAIRNQNTAIEKLLSRVLDEAGITYRTQASDLPGKPDFVIDDYKAI
CCCCCCCCCCCCHHHHHHCCCHHHHHHHHHHHHHCCCEEEECCCCCCCCCCEEECCCEEE
IFVHGCFWHHHDCYLFKVPATRTEFWLKKIDSNVRRDNDVTRLLTEQGWRELIIWECSLK
EEEEEHEEECCCEEEEECCCCHHHHHHHHHHHHCCCCHHHHHHHHHCCCEEEEEEEECCC
GRIKLSANALSERIEEWLCASDTHAVIDSKGIHQLNN
CEEEECHHHHHHHHHHHHCCCCCCHHHCCCCCCCCCC
>Mature Secondary Structure 
ADVHSPSIRSKNMRAIRNQNTAIEKLLSRVLDEAGITYRTQASDLPGKPDFVIDDYKAI
CCCCCCCCCCCHHHHHHCCCHHHHHHHHHHHHHCCCEEEECCCCCCCCCCEEECCCEEE
IFVHGCFWHHHDCYLFKVPATRTEFWLKKIDSNVRRDNDVTRLLTEQGWRELIIWECSLK
EEEEEHEEECCCEEEEECCCCHHHHHHHHHHHHCCCCHHHHHHHHHCCCEEEEEEEECCC
GRIKLSANALSERIEEWLCASDTHAVIDSKGIHQLNN
CEEEECHHHHHHHHHHHHCCCCCCHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: DNA mismatch endonuclease vsr specifically recognizes a T-G mismatch in a special context related to the dcm methylation sequence and makes an incision 5' to the mismatched T residue [C]

General reaction: Hydrolase; Acting on ester bonds [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2527357; 2198248; 9097040; 9278503; 1944537; 10871403; 10612397; 10360178 [H]