Click here to switch to the map view.

The map label for this gene is ecoRIIM [H]

Identifier: 157368818

GI number: 157368818

Start: 636066

End: 637541

Strand: Reverse

Name: ecoRIIM [H]

Synonym: Spro_0573

Alternate gene names: 157368818

Gene position: 637541-636066 (Counterclockwise)

Preceding gene: 157368819

Following gene: 157368817

Centisome position: 11.7

GC content: 43.63

Gene sequence:

>1476_bases
ATGAGTGATAGGCTATCACTAAGTCATTCCACATTTGGAAGAGTCATTATGCAAGAGTTCCATGAGCGTGCAAAAAATTT
CCGCGCTGAAGCACAAGAACAACAGTTAGCCCAGCAGGAAGAAGATAAGGCACTAGTTGAAAAACTGGTTGGAATTTACG
ATCAGAAAGCCATCGCCGAACTTCTGCGTGAAGCCAGTGGTAGCGAATGGACACGTGAGTCTTTGAATCGTTGGCTTAAA
GGAAAAACACCGCAGAAAGTATTGATAGACGCTGAGGTGAAATTACTGCGATCAATGTTGCCAAAACCCTCATCGTTTCA
CCCTAACTATAAGTTCCGTTTTATCGACCTGTTTGCAGGCATTGGTGGAATTCGTAAAGGGTTCGAAGAGATCGGTGGTC
AATGCGTGTTTACCAGTGAATGGAACAAAGAAGCTGTTCGCACCTACAAAGCTAACTGGTACTGTGACCCTGCACATCAC
AAGTTCAACTCTGATATTCGCGAGATCACCCTCAGCGAGAATGATGATATTTCCGATCAGGAAGCATATAAAAATATCGA
TAAAGAAATCCCTGATCATGATGTTTTGTTGGCAGGTTTTCCATGCCAGCCTTTTTCCCTGGCAGGAGTATCTAAGAAGA
ACTCTCTGGGGAGAGCTCACGGTTTTGAGTGTGAAACTCAGGGGACATTGTTCTTCGACGTAGCCCGGATTATTGCTGCG
AAAAAACCTGCAATTTTCCTTCTCGAAAACGTCAAAAACCTTAAGAGCCATGATAAAGGGAAAACCTTCAAAATTATCAT
GGAAACACTGGATGAACTGGGTTACGAAGTTGCTGATGCCCAGGCTACCGGCCCTAGTGATCCTAAAATCATCGATGGGA
AAAATTTCTTACCTCAACACCGCGAACGTATCGTATTGGTTGGGTTCCGTCGTGATTTGAATATTCATGGCGATTTTTCA
CTCAAAAACTTGAGTCAGTTCTTTCCGAAGAAAAGACCATCATTTGGTGAATTACTACTCGAAGATGTTGCTCCAAAGTA
CATTCTGACAGAGCATCTGTGGAAATATCTTTATAACTATGCAAAAAAACACCAGGCAAAAGGCAATGGTTTTGGATTTG
GTTTAGTAAATCCGAAAAATGAATCGAGCGTTGCCAGAACGCTATCAGCCCGCTATCACAAAGATGGTTCAGAGATCCTG
ATTGATCGAGGATGGGACATGGAACTTGGTGAAAAAGATTTCGCAGATCCTGAAAATCAAGCCAATCGACCTCGCCGTTT
GACACCAAAAGAATGTGCCCGAATTATGGGGTTCGAAACTCCAGGTGGAGCAGACTTCTACATCCCGGTATCAGATACCC
AAGCGTATCGGCAGTTCGGTAACTCAGTCATTGTGCCGGCATTTGCAGCCGTAGCTAAGTTACTAGAACCTTACATTGCT
TCGGCTGTTTCAAAAGACAAAAGTGAAGCCGCATAA

Upstream 100 bases:

>100_bases
GGGCAGCTTCTGAGAGTTGCCCTGTTGCATTCGGGCCCAGACGACACTCATTACAGGCATTGTACTGGATATTCATCCAT
ACATCTTTTAACTGGGTATA

Downstream 100 bases:

>100_bases
GGTAAATTATGGCTGACGTACACTCCCCCTCAATCAGAAGCAAGAACATGAGGGCGATACGTAATCAAAATACAGCCATT
GAGAAATTGTTATCCCGGGT

Product: DNA cytosine methylase

Products: NA

Alternate protein names: M.EcoRII; Cytosine-specific methyltransferase EcoRII [H]

Number of amino acids: Translated: 491; Mature: 490

Protein sequence:

>491_residues
MSDRLSLSHSTFGRVIMQEFHERAKNFRAEAQEQQLAQQEEDKALVEKLVGIYDQKAIAELLREASGSEWTRESLNRWLK
GKTPQKVLIDAEVKLLRSMLPKPSSFHPNYKFRFIDLFAGIGGIRKGFEEIGGQCVFTSEWNKEAVRTYKANWYCDPAHH
KFNSDIREITLSENDDISDQEAYKNIDKEIPDHDVLLAGFPCQPFSLAGVSKKNSLGRAHGFECETQGTLFFDVARIIAA
KKPAIFLLENVKNLKSHDKGKTFKIIMETLDELGYEVADAQATGPSDPKIIDGKNFLPQHRERIVLVGFRRDLNIHGDFS
LKNLSQFFPKKRPSFGELLLEDVAPKYILTEHLWKYLYNYAKKHQAKGNGFGFGLVNPKNESSVARTLSARYHKDGSEIL
IDRGWDMELGEKDFADPENQANRPRRLTPKECARIMGFETPGGADFYIPVSDTQAYRQFGNSVIVPAFAAVAKLLEPYIA
SAVSKDKSEAA

Sequences:

>Translated_491_residues
MSDRLSLSHSTFGRVIMQEFHERAKNFRAEAQEQQLAQQEEDKALVEKLVGIYDQKAIAELLREASGSEWTRESLNRWLK
GKTPQKVLIDAEVKLLRSMLPKPSSFHPNYKFRFIDLFAGIGGIRKGFEEIGGQCVFTSEWNKEAVRTYKANWYCDPAHH
KFNSDIREITLSENDDISDQEAYKNIDKEIPDHDVLLAGFPCQPFSLAGVSKKNSLGRAHGFECETQGTLFFDVARIIAA
KKPAIFLLENVKNLKSHDKGKTFKIIMETLDELGYEVADAQATGPSDPKIIDGKNFLPQHRERIVLVGFRRDLNIHGDFS
LKNLSQFFPKKRPSFGELLLEDVAPKYILTEHLWKYLYNYAKKHQAKGNGFGFGLVNPKNESSVARTLSARYHKDGSEIL
IDRGWDMELGEKDFADPENQANRPRRLTPKECARIMGFETPGGADFYIPVSDTQAYRQFGNSVIVPAFAAVAKLLEPYIA
SAVSKDKSEAA
>Mature_490_residues
SDRLSLSHSTFGRVIMQEFHERAKNFRAEAQEQQLAQQEEDKALVEKLVGIYDQKAIAELLREASGSEWTRESLNRWLKG
KTPQKVLIDAEVKLLRSMLPKPSSFHPNYKFRFIDLFAGIGGIRKGFEEIGGQCVFTSEWNKEAVRTYKANWYCDPAHHK
FNSDIREITLSENDDISDQEAYKNIDKEIPDHDVLLAGFPCQPFSLAGVSKKNSLGRAHGFECETQGTLFFDVARIIAAK
KPAIFLLENVKNLKSHDKGKTFKIIMETLDELGYEVADAQATGPSDPKIIDGKNFLPQHRERIVLVGFRRDLNIHGDFSL
KNLSQFFPKKRPSFGELLLEDVAPKYILTEHLWKYLYNYAKKHQAKGNGFGFGLVNPKNESSVARTLSARYHKDGSEILI
DRGWDMELGEKDFADPENQANRPRRLTPKECARIMGFETPGGADFYIPVSDTQAYRQFGNSVIVPAFAAVAKLLEPYIAS
AVSKDKSEAA

Specific function: This methylase recognizes the double-stranded sequence CCWGG, causes specific methylation on C-2 on both strands, and protects the DNA from cleavage by the EcoRII endonuclease [H]

COG id: COG0270

COG function: function code L; Site-specific DNA methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the C5-methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1788271, Length=448, Percent_Identity=68.5267857142857, Blast_Score=630, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018117
- InterPro:   IPR001525 [H]

Pfam domain/function: PF00145 DNA_methylase [H]

EC number: =2.1.1.37 [H]

Molecular weight: Translated: 55588; Mature: 55456

Theoretical pI: Translated: 7.67; Mature: 7.67

Prosite motif: PS00094 C5_MTASE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDRLSLSHSTFGRVIMQEFHERAKNFRAEAQEQQLAQQEEDKALVEKLVGIYDQKAIAE
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHH
LLREASGSEWTRESLNRWLKGKTPQKVLIDAEVKLLRSMLPKPSSFHPNYKFRFIDLFAG
HHHHCCCCHHHHHHHHHHHCCCCCCEEEEHHHHHHHHHHCCCCCCCCCCCEEEHHHHHHH
IGGIRKGFEEIGGQCVFTSEWNKEAVRTYKANWYCDPAHHKFNSDIREITLSENDDISDQ
HHHHHHHHHHHCCEEEEECCCCHHHHHHCCCCEEECHHHHHHCCHHHEEECCCCCCCCHH
EAYKNIDKEIPDHDVLLAGFPCQPFSLAGVSKKNSLGRAHGFECETQGTLFFDVARIIAA
HHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHC
KKPAIFLLENVKNLKSHDKGKTFKIIMETLDELGYEVADAQATGPSDPKIIDGKNFLPQH
CCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCEECCCCCCCCCCCCEECCCCCCCCC
RERIVLVGFRRDLNIHGDFSLKNLSQFFPKKRPSFGELLLEDVAPKYILTEHLWKYLYNY
CCEEEEEEEECCCCCCCCCCHHHHHHHCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHH
AKKHQAKGNGFGFGLVNPKNESSVARTLSARYHKDGSEILIDRGWDMELGEKDFADPENQ
HHHHCCCCCCEEEECCCCCCHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCHHH
ANRPRRLTPKECARIMGFETPGGADFYIPVSDTQAYRQFGNSVIVPAFAAVAKLLEPYIA
CCCCCCCCHHHHHHHHCCCCCCCCEEEEECCCHHHHHHHCCCEEHHHHHHHHHHHHHHHH
SAVSKDKSEAA
HHHHCCCCCCC
>Mature Secondary Structure 
SDRLSLSHSTFGRVIMQEFHERAKNFRAEAQEQQLAQQEEDKALVEKLVGIYDQKAIAE
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHH
LLREASGSEWTRESLNRWLKGKTPQKVLIDAEVKLLRSMLPKPSSFHPNYKFRFIDLFAG
HHHHCCCCHHHHHHHHHHHCCCCCCEEEEHHHHHHHHHHCCCCCCCCCCCEEEHHHHHHH
IGGIRKGFEEIGGQCVFTSEWNKEAVRTYKANWYCDPAHHKFNSDIREITLSENDDISDQ
HHHHHHHHHHHCCEEEEECCCCHHHHHHCCCCEEECHHHHHHCCHHHEEECCCCCCCCHH
EAYKNIDKEIPDHDVLLAGFPCQPFSLAGVSKKNSLGRAHGFECETQGTLFFDVARIIAA
HHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHC
KKPAIFLLENVKNLKSHDKGKTFKIIMETLDELGYEVADAQATGPSDPKIIDGKNFLPQH
CCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCEECCCCCCCCCCCCEECCCCCCCCC
RERIVLVGFRRDLNIHGDFSLKNLSQFFPKKRPSFGELLLEDVAPKYILTEHLWKYLYNY
CCEEEEEEEECCCCCCCCCCHHHHHHHCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHH
AKKHQAKGNGFGFGLVNPKNESSVARTLSARYHKDGSEILIDRGWDMELGEKDFADPENQ
HHHHCCCCCCEEEECCCCCCHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCHHH
ANRPRRLTPKECARIMGFETPGGADFYIPVSDTQAYRQFGNSVIVPAFAAVAKLLEPYIA
CCCCCCCCHHHHHHHHCCCCCCCCEEEEECCCHHHHHHHCCCEEHHHHHHHHHHHHHHHH
SAVSKDKSEAA
HHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3029675 [H]