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The map label for this gene is ecoRIIR [H]

Identifier: 157368816

GI number: 157368816

Start: 634208

End: 635422

Strand: Reverse

Name: ecoRIIR [H]

Synonym: Spro_0571

Alternate gene names: 157368816

Gene position: 635422-634208 (Counterclockwise)

Preceding gene: 157368817

Following gene: 157368815

Centisome position: 11.66

GC content: 37.7

Gene sequence:

>1215_bases
GTGTCTGATTTTCAAAATTGGTTAACGGAAAAATCATCGGATAATTGGTACATACATATCAAAAGGCTCTCGGCAAATGA
TACCGGAGCTAGTGGTGGCCATCAATCTGGTGTTTATCTTCCTAATAATTCAGCAAATTTTCTTTTCCCGTCAATTAACC
GAATTGATGAAGAAAATCCATCAGTAGGTCTTACAGCAAAAATAATTTCCCATGAAGTCAATGACTCTCAGGTAAGAGCT
GTATATTACAACAGTAAACTAAGAAACCTTAACCCAAAGGGCCGTAATGAAACTCGTATTACCTGCTGGGGTGGACAAAA
AAGCCCGGTACAGAACCAAAATAATACCGGTGCTATAGCTATATTTGCTTTCTATACGCCTGAAAACTTGAAAGACTGTG
GCCTTGTTGAAGCATGGGTATGCTGTACTGTTGAAGAAGAAGATTTACTCGAAGCAATTATTGGCGAAGTGCTTCCTGGG
ACGGCGCGATCTGGTCCATCAGCTCAGATATTAGGCGGTTTCGCTATTGATGATAGTGATTGGAAATCTCATAATTACTC
AATACCTGACCCATGGAAAGTAACGTTCCCTAGTGGTAGTGATATTATCGAGTATCTTCCAAATATCTTTAAATTCAAAG
GTGACACTCCAGATAAATTGTTACTAGAACGACGCGAGAAAGAGTATTCATTGTTCCGGCAAATTGAAGAAATTCATGTT
CTAGATAAAATAAAGAAAGGTTTCAATTCTGTAGAAGAATTCATTGAACTTGCGAATTCAGTTAGTAATCGTCGAAAATC
CCGTTCAGGAAAATCACTTGAGATGCATCTTGAGAAAATATTTCTTGCGAATGGACTTACAACGTTTACTACGCAAAGTA
TTACTGAGGGTAAAAAAAAGCCTGATTTTCTTTTCCCATCCGGAATTGAATATCATAACCCAGCATACCCCGCAGAAAAT
CTAAGGATGTTAGCAGTTAAAACAACCTGTAAGGATCGGTGGAGACAGGCGATTAATGAAGCAAACAGGATTAACAATAT
ACACTTATTTACTCTACAAGAAGGTGTTTCTGCCAATCAATTTCAAGAAATGAAAGATGCCGGAGTTACACTTGTTGTTC
CTGAGCCTTTGCACAAAAAATACCCTGAAAAAATCAGAAGTGAATTAGTTTCATTGAGTGATTTCATTAGCGAGACTTTA
GCCATATATACATAA

Upstream 100 bases:

>100_bases
AACTTTGTCTCTCATCATCGACATCTATTCAATAACGTGGATAATAAATTCATAACAATAGCTGGGTGGTATACATACCG
ATATCGGTGAGAGGGATCAA

Downstream 100 bases:

>100_bases
AATCACAACATTAACGAACATTCAATTAATGGTTTATATCATCGTACCCAGCCACACTACCCTAAGACACATAATAAAGG
ATACTTTAGATGGCATCATC

Product: Type II site-specific deoxyribonuclease

Products: NA

Alternate protein names: R.EcoRII; Endonuclease EcoRII; Type II restriction enzyme EcoRII [H]

Number of amino acids: Translated: 404; Mature: 403

Protein sequence:

>404_residues
MSDFQNWLTEKSSDNWYIHIKRLSANDTGASGGHQSGVYLPNNSANFLFPSINRIDEENPSVGLTAKIISHEVNDSQVRA
VYYNSKLRNLNPKGRNETRITCWGGQKSPVQNQNNTGAIAIFAFYTPENLKDCGLVEAWVCCTVEEEDLLEAIIGEVLPG
TARSGPSAQILGGFAIDDSDWKSHNYSIPDPWKVTFPSGSDIIEYLPNIFKFKGDTPDKLLLERREKEYSLFRQIEEIHV
LDKIKKGFNSVEEFIELANSVSNRRKSRSGKSLEMHLEKIFLANGLTTFTTQSITEGKKKPDFLFPSGIEYHNPAYPAEN
LRMLAVKTTCKDRWRQAINEANRINNIHLFTLQEGVSANQFQEMKDAGVTLVVPEPLHKKYPEKIRSELVSLSDFISETL
AIYT

Sequences:

>Translated_404_residues
MSDFQNWLTEKSSDNWYIHIKRLSANDTGASGGHQSGVYLPNNSANFLFPSINRIDEENPSVGLTAKIISHEVNDSQVRA
VYYNSKLRNLNPKGRNETRITCWGGQKSPVQNQNNTGAIAIFAFYTPENLKDCGLVEAWVCCTVEEEDLLEAIIGEVLPG
TARSGPSAQILGGFAIDDSDWKSHNYSIPDPWKVTFPSGSDIIEYLPNIFKFKGDTPDKLLLERREKEYSLFRQIEEIHV
LDKIKKGFNSVEEFIELANSVSNRRKSRSGKSLEMHLEKIFLANGLTTFTTQSITEGKKKPDFLFPSGIEYHNPAYPAEN
LRMLAVKTTCKDRWRQAINEANRINNIHLFTLQEGVSANQFQEMKDAGVTLVVPEPLHKKYPEKIRSELVSLSDFISETL
AIYT
>Mature_403_residues
SDFQNWLTEKSSDNWYIHIKRLSANDTGASGGHQSGVYLPNNSANFLFPSINRIDEENPSVGLTAKIISHEVNDSQVRAV
YYNSKLRNLNPKGRNETRITCWGGQKSPVQNQNNTGAIAIFAFYTPENLKDCGLVEAWVCCTVEEEDLLEAIIGEVLPGT
ARSGPSAQILGGFAIDDSDWKSHNYSIPDPWKVTFPSGSDIIEYLPNIFKFKGDTPDKLLLERREKEYSLFRQIEEIHVL
DKIKKGFNSVEEFIELANSVSNRRKSRSGKSLEMHLEKIFLANGLTTFTTQSITEGKKKPDFLFPSGIEYHNPAYPAENL
RMLAVKTTCKDRWRQAINEANRINNIHLFTLQEGVSANQFQEMKDAGVTLVVPEPLHKKYPEKIRSELVSLSDFISETLA
IYT

Specific function: Recognizes the double-stranded sequence CCWGG and cleaves before C-1 [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011335
- InterPro:   IPR015109
- InterPro:   IPR015300 [H]

Pfam domain/function: PF09019 EcoRII-C; PF09217 EcoRII-N [H]

EC number: =3.1.21.4 [H]

Molecular weight: Translated: 45644; Mature: 45513

Theoretical pI: Translated: 6.26; Mature: 6.26

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDFQNWLTEKSSDNWYIHIKRLSANDTGASGGHQSGVYLPNNSANFLFPSINRIDEENP
CCHHHHHHHCCCCCCEEEEEEEECCCCCCCCCCCCCCEEECCCCCCEECCCHHHCCCCCC
SVGLTAKIISHEVNDSQVRAVYYNSKLRNLNPKGRNETRITCWGGQKSPVQNQNNTGAIA
CCCEEEHHHHCCCCCCEEEEEEECCHHCCCCCCCCCCCEEEEECCCCCCCCCCCCCCEEE
IFAFYTPENLKDCGLVEAWVCCTVEEEDLLEAIIGEVLPGTARSGPSAQILGGFAIDDSD
EEEEECCCCCCCCCCEEEEEEEECCHHHHHHHHHHHHCCCCCCCCCCCEEEEEEEECCCC
WKSHNYSIPDPWKVTFPSGSDIIEYLPNIFKFKGDTPDKLLLERREKEYSLFRQIEEIHV
CCCCCCCCCCCEEEECCCCCHHHHHHHHHHEECCCCHHHHHHHHHHHHHHHHHHHHHHHH
LDKIKKGFNSVEEFIELANSVSNRRKSRSGKSLEMHLEKIFLANGLTTFTTQSITEGKKK
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCHHHHHHHHHCCCCC
PDFLFPSGIEYHNPAYPAENLRMLAVKTTCKDRWRQAINEANRINNIHLFTLQEGVSANQ
CCCCCCCCCCCCCCCCCCCCEEEEEEEHHHHHHHHHHHHHHHHCCCEEEEEECCCCCHHH
FQEMKDAGVTLVVPEPLHKKYPEKIRSELVSLSDFISETLAIYT
HHHHHHCCCEEEECCHHHHHCHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SDFQNWLTEKSSDNWYIHIKRLSANDTGASGGHQSGVYLPNNSANFLFPSINRIDEENP
CHHHHHHHCCCCCCEEEEEEEECCCCCCCCCCCCCCEEECCCCCCEECCCHHHCCCCCC
SVGLTAKIISHEVNDSQVRAVYYNSKLRNLNPKGRNETRITCWGGQKSPVQNQNNTGAIA
CCCEEEHHHHCCCCCCEEEEEEECCHHCCCCCCCCCCCEEEEECCCCCCCCCCCCCCEEE
IFAFYTPENLKDCGLVEAWVCCTVEEEDLLEAIIGEVLPGTARSGPSAQILGGFAIDDSD
EEEEECCCCCCCCCCEEEEEEEECCHHHHHHHHHHHHCCCCCCCCCCCEEEEEEEECCCC
WKSHNYSIPDPWKVTFPSGSDIIEYLPNIFKFKGDTPDKLLLERREKEYSLFRQIEEIHV
CCCCCCCCCCCEEEECCCCCHHHHHHHHHHEECCCCHHHHHHHHHHHHHHHHHHHHHHHH
LDKIKKGFNSVEEFIELANSVSNRRKSRSGKSLEMHLEKIFLANGLTTFTTQSITEGKKK
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCHHHHHHHHHCCCCC
PDFLFPSGIEYHNPAYPAENLRMLAVKTTCKDRWRQAINEANRINNIHLFTLQEGVSANQ
CCCCCCCCCCCCCCCCCCCCEEEEEEEHHHHHHHHHHHHHHHHCCCEEEEEECCCCCHHH
FQEMKDAGVTLVVPEPLHKKYPEKIRSELVSLSDFISETLAIYT
HHHHHHCCCEEEECCHHHHHCHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2104830; 2597679; 2612358; 8392701 [H]