BacMap

Definition	Escherichia coli HS, complete genome.
Accession	NC_009800
Length	4,643,538

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The map label for this gene is idnO

Identifier: 157163741

GI number: 157163741

Start: 4528716

End: 4529480

Strand: Reverse

Name: idnO

Synonym: EcHS_A4522

Alternate gene names: 157163741

Gene position: 4529480-4528716 (Counterclockwise)

Preceding gene: 157163742

Following gene: 157163740

Centisome position: 97.54

GC content: 49.41

Gene sequence:

>765_bases
ATGAACGATCTATTTTCACTGGCAGGAAAAAATATCTTGATTACCGGTTCAGCACAGGGCATTGGCTTTTTACTGGCAAC
CGGCCTGGGTAAATATGGCGCACAAATAATTATTAATGATATTACTGCCGAACGCGCAGAACTTGCTGTAGAAAAACTCC
ACCAGGAGGGTATTCAGGCCGTTGCCGCACCTTTTAATGTTACTCATAAACATGAAATTGATGCCGCCGTTGAACATATC
GAAAAGGACATCGGCCCCATTGATGTGCTGGTGAATAACGCCGGTATCCAGCGCCGTCATCCTTTTACTGAGTTCCCTGA
ACAAGAGTGGAATGATGTGATCGCAGTAAACCAGACCGCCGTGTTCCTGGTATCGCAAGCGGTAACTCGTCACATGGTTG
AACGCAAGGCAGGTAAAGTTATTAATATTTGCTCGATGCAAAGCGAACTGGGACGTGACACCATCACCCCTTATGCCGCA
TCGAAAGGGGCGGTAAAAATGCTCACCCGCGGCATGTGCGTCGAGCTGGCGCGCCACAATATTCAGGTCAACGGTATTGC
GCCGGGCTATTTCAAAACAGAAATGACTAAAGCACTGGTTGAGGACGAAGCCTTCACCGCCTGGTTGTGCAAACGGACCC
CCGCCGCACGCTGGGGAGATCCGCAGGAACTGATTGGTGCTGCGGTGTTCCTTTCTTCAAAAGCCTCTGATTTCGTAAAC
GGCCACCTGTTGTTTGTTGATGGCGGCATGTTAGTGGCTGTTTAA

Upstream 100 bases:

>100_bases
CCTTCACTGACCTGGAAGAGGCGCTACGTTTCGCCGGTGATAAAACCCAGGCAGCAAAAGTCCAGCTTGTTTTCTAAGAG
ATAAATAAAGGAATAATACA

Downstream 100 bases:

>100_bases
CACCTACTACCCTACGTGCAGCGCGAAAGCGCTGCTTTTCTGGCACTAATAAGAGATAAAGACTATGCCATTAATCATTA
TTGCGGCAGGCGTCGCGCTG

Product: gluconate 5-dehydrogenase

Products: NA

Alternate protein names: 5-keto-D-gluconate 5-reductase

Number of amino acids: Translated: 254; Mature: 254

Protein sequence:

>254_residues
MNDLFSLAGKNILITGSAQGIGFLLATGLGKYGAQIIINDITAERAELAVEKLHQEGIQAVAAPFNVTHKHEIDAAVEHI
EKDIGPIDVLVNNAGIQRRHPFTEFPEQEWNDVIAVNQTAVFLVSQAVTRHMVERKAGKVINICSMQSELGRDTITPYAA
SKGAVKMLTRGMCVELARHNIQVNGIAPGYFKTEMTKALVEDEAFTAWLCKRTPAARWGDPQELIGAAVFLSSKASDFVN
GHLLFVDGGMLVAV

Sequences:

>Translated_254_residues
MNDLFSLAGKNILITGSAQGIGFLLATGLGKYGAQIIINDITAERAELAVEKLHQEGIQAVAAPFNVTHKHEIDAAVEHI
EKDIGPIDVLVNNAGIQRRHPFTEFPEQEWNDVIAVNQTAVFLVSQAVTRHMVERKAGKVINICSMQSELGRDTITPYAA
SKGAVKMLTRGMCVELARHNIQVNGIAPGYFKTEMTKALVEDEAFTAWLCKRTPAARWGDPQELIGAAVFLSSKASDFVN
GHLLFVDGGMLVAV
>Mature_254_residues
MNDLFSLAGKNILITGSAQGIGFLLATGLGKYGAQIIINDITAERAELAVEKLHQEGIQAVAAPFNVTHKHEIDAAVEHI
EKDIGPIDVLVNNAGIQRRHPFTEFPEQEWNDVIAVNQTAVFLVSQAVTRHMVERKAGKVINICSMQSELGRDTITPYAA
SKGAVKMLTRGMCVELARHNIQVNGIAPGYFKTEMTKALVEDEAFTAWLCKRTPAARWGDPQELIGAAVFLSSKASDFVN
GHLLFVDGGMLVAV

Specific function: Catalyzes a reversible reduction of 5-ketoglutanate to form D-gluconate. Dependent on NADP, almost inactive with NAD

COG id: COG1028

COG function: function code IQR; Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family

Homologues:

Organism=Homo sapiens, GI32483357, Length=247, Percent_Identity=30.3643724696356, Blast_Score=116, Evalue=2e-26,
Organism=Homo sapiens, GI7705925, Length=250, Percent_Identity=31.2, Blast_Score=115, Evalue=3e-26,
Organism=Homo sapiens, GI40254992, Length=237, Percent_Identity=30.8016877637131, Blast_Score=114, Evalue=1e-25,
Organism=Homo sapiens, GI15277342, Length=249, Percent_Identity=29.718875502008, Blast_Score=104, Evalue=7e-23,
Organism=Homo sapiens, GI19923817, Length=250, Percent_Identity=28.4, Blast_Score=99, Evalue=4e-21,
Organism=Homo sapiens, GI126723750, Length=247, Percent_Identity=27.9352226720648, Blast_Score=96, Evalue=2e-20,
Organism=Homo sapiens, GI5031737, Length=247, Percent_Identity=28.7449392712551, Blast_Score=96, Evalue=3e-20,
Organism=Homo sapiens, GI10190704, Length=245, Percent_Identity=27.3469387755102, Blast_Score=91, Evalue=8e-19,
Organism=Homo sapiens, GI59889578, Length=227, Percent_Identity=31.2775330396476, Blast_Score=91, Evalue=1e-18,
Organism=Homo sapiens, GI4504505, Length=233, Percent_Identity=27.0386266094421, Blast_Score=88, Evalue=8e-18,
Organism=Homo sapiens, GI66933014, Length=249, Percent_Identity=29.3172690763052, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI33667109, Length=198, Percent_Identity=29.7979797979798, Blast_Score=82, Evalue=4e-16,
Organism=Homo sapiens, GI210032110, Length=195, Percent_Identity=26.1538461538462, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI4758504, Length=258, Percent_Identity=28.6821705426357, Blast_Score=79, Evalue=5e-15,
Organism=Homo sapiens, GI126723191, Length=182, Percent_Identity=29.6703296703297, Blast_Score=77, Evalue=1e-14,
Organism=Homo sapiens, GI7706318, Length=190, Percent_Identity=26.8421052631579, Blast_Score=76, Evalue=2e-14,
Organism=Homo sapiens, GI4503817, Length=207, Percent_Identity=23.1884057971014, Blast_Score=74, Evalue=9e-14,
Organism=Homo sapiens, GI226371731, Length=211, Percent_Identity=28.9099526066351, Blast_Score=73, Evalue=2e-13,
Organism=Homo sapiens, GI209862901, Length=199, Percent_Identity=28.643216080402, Blast_Score=73, Evalue=3e-13,
Organism=Homo sapiens, GI19923983, Length=199, Percent_Identity=28.643216080402, Blast_Score=73, Evalue=3e-13,
Organism=Homo sapiens, GI4503301, Length=226, Percent_Identity=23.8938053097345, Blast_Score=72, Evalue=6e-13,
Organism=Homo sapiens, GI40807363, Length=194, Percent_Identity=26.2886597938144, Blast_Score=71, Evalue=9e-13,
Organism=Homo sapiens, GI20149619, Length=191, Percent_Identity=26.7015706806283, Blast_Score=71, Evalue=1e-12,
Organism=Homo sapiens, GI83715985, Length=259, Percent_Identity=27.7992277992278, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI40254986, Length=194, Percent_Identity=29.3814432989691, Blast_Score=67, Evalue=2e-11,
Organism=Escherichia coli, GI1790717, Length=254, Percent_Identity=100, Blast_Score=525, Evalue=1e-150,
Organism=Escherichia coli, GI87082160, Length=250, Percent_Identity=40.8, Blast_Score=192, Evalue=2e-50,
Organism=Escherichia coli, GI1789208, Length=248, Percent_Identity=41.9354838709677, Blast_Score=180, Evalue=8e-47,
Organism=Escherichia coli, GI1787335, Length=242, Percent_Identity=35.5371900826446, Blast_Score=156, Evalue=1e-39,
Organism=Escherichia coli, GI1787905, Length=248, Percent_Identity=35.8870967741936, Blast_Score=135, Evalue=2e-33,
Organism=Escherichia coli, GI87082100, Length=250, Percent_Identity=34.4, Blast_Score=133, Evalue=1e-32,
Organism=Escherichia coli, GI1788459, Length=243, Percent_Identity=32.5102880658436, Blast_Score=117, Evalue=8e-28,
Organism=Escherichia coli, GI2367175, Length=243, Percent_Identity=29.2181069958848, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1786812, Length=253, Percent_Identity=25.6916996047431, Blast_Score=91, Evalue=7e-20,
Organism=Escherichia coli, GI1789378, Length=246, Percent_Identity=26.8292682926829, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI1789057, Length=253, Percent_Identity=22.5296442687747, Blast_Score=81, Evalue=7e-17,
Organism=Escherichia coli, GI2367365, Length=245, Percent_Identity=26.9387755102041, Blast_Score=71, Evalue=8e-14,
Organism=Escherichia coli, GI1787526, Length=191, Percent_Identity=28.7958115183246, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI1787820, Length=179, Percent_Identity=26.2569832402235, Blast_Score=60, Evalue=1e-10,
Organism=Caenorhabditis elegans, GI25147288, Length=245, Percent_Identity=32.6530612244898, Blast_Score=129, Evalue=1e-30,
Organism=Caenorhabditis elegans, GI17555706, Length=245, Percent_Identity=31.0204081632653, Blast_Score=115, Evalue=3e-26,
Organism=Caenorhabditis elegans, GI17560676, Length=245, Percent_Identity=26.9387755102041, Blast_Score=114, Evalue=6e-26,
Organism=Caenorhabditis elegans, GI115534694, Length=252, Percent_Identity=32.5396825396825, Blast_Score=114, Evalue=7e-26,
Organism=Caenorhabditis elegans, GI17561402, Length=247, Percent_Identity=28.7449392712551, Blast_Score=104, Evalue=5e-23,
Organism=Caenorhabditis elegans, GI193203117, Length=194, Percent_Identity=27.319587628866, Blast_Score=99, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI193203115, Length=189, Percent_Identity=28.042328042328, Blast_Score=97, Evalue=5e-21,
Organism=Caenorhabditis elegans, GI71994600, Length=245, Percent_Identity=29.3877551020408, Blast_Score=97, Evalue=6e-21,
Organism=Caenorhabditis elegans, GI71994604, Length=263, Percent_Identity=31.1787072243346, Blast_Score=96, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17560150, Length=257, Percent_Identity=27.6264591439689, Blast_Score=95, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI72000259, Length=263, Percent_Identity=28.5171102661597, Blast_Score=93, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17562990, Length=259, Percent_Identity=28.5714285714286, Blast_Score=91, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI17559104, Length=261, Percent_Identity=28.735632183908, Blast_Score=91, Evalue=8e-19,
Organism=Caenorhabditis elegans, GI17563726, Length=259, Percent_Identity=27.7992277992278, Blast_Score=90, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17562904, Length=259, Percent_Identity=28.5714285714286, Blast_Score=89, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17564282, Length=161, Percent_Identity=31.6770186335404, Blast_Score=89, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17562910, Length=257, Percent_Identity=26.0700389105058, Blast_Score=86, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17562906, Length=259, Percent_Identity=25.4826254826255, Blast_Score=84, Evalue=6e-17,
Organism=Caenorhabditis elegans, GI17538182, Length=264, Percent_Identity=30.6818181818182, Blast_Score=84, Evalue=9e-17,
Organism=Caenorhabditis elegans, GI17568967, Length=189, Percent_Identity=29.1005291005291, Blast_Score=83, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17560220, Length=264, Percent_Identity=26.5151515151515, Blast_Score=82, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI17562908, Length=264, Percent_Identity=24.6212121212121, Blast_Score=79, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17538486, Length=254, Percent_Identity=26.7716535433071, Blast_Score=79, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17536025, Length=251, Percent_Identity=25.0996015936255, Blast_Score=78, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI193204405, Length=261, Percent_Identity=25.6704980842912, Blast_Score=77, Evalue=6e-15,
Organism=Caenorhabditis elegans, GI17531453, Length=261, Percent_Identity=26.0536398467433, Blast_Score=77, Evalue=7e-15,
Organism=Caenorhabditis elegans, GI17544670, Length=269, Percent_Identity=24.5353159851301, Blast_Score=77, Evalue=8e-15,
Organism=Caenorhabditis elegans, GI17551412, Length=254, Percent_Identity=25.9842519685039, Blast_Score=77, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17565030, Length=263, Percent_Identity=27.7566539923954, Blast_Score=76, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17538480, Length=261, Percent_Identity=25.6704980842912, Blast_Score=75, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI17561272, Length=237, Percent_Identity=26.1603375527426, Blast_Score=74, Evalue=6e-14,
Organism=Caenorhabditis elegans, GI17508895, Length=195, Percent_Identity=26.6666666666667, Blast_Score=74, Evalue=8e-14,
Organism=Caenorhabditis elegans, GI17536651, Length=251, Percent_Identity=23.5059760956175, Blast_Score=74, Evalue=9e-14,
Organism=Caenorhabditis elegans, GI17560332, Length=255, Percent_Identity=25.0980392156863, Blast_Score=71, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI71997099, Length=223, Percent_Identity=28.2511210762332, Blast_Score=70, Evalue=7e-13,
Organism=Caenorhabditis elegans, GI17555612, Length=217, Percent_Identity=26.7281105990783, Blast_Score=68, Evalue=5e-12,
Organism=Caenorhabditis elegans, GI193210355, Length=196, Percent_Identity=26.0204081632653, Blast_Score=66, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI115534660, Length=198, Percent_Identity=27.2727272727273, Blast_Score=65, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI71982365, Length=191, Percent_Identity=28.7958115183246, Blast_Score=64, Evalue=1e-10,
Organism=Saccharomyces cerevisiae, GI6322861, Length=193, Percent_Identity=31.0880829015544, Blast_Score=89, Evalue=6e-19,
Organism=Saccharomyces cerevisiae, GI6323882, Length=198, Percent_Identity=30.8080808080808, Blast_Score=69, Evalue=9e-13,
Organism=Saccharomyces cerevisiae, GI6324126, Length=253, Percent_Identity=26.4822134387352, Blast_Score=64, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6320089, Length=199, Percent_Identity=29.1457286432161, Blast_Score=63, Evalue=4e-11,
Organism=Drosophila melanogaster, GI24639444, Length=247, Percent_Identity=33.1983805668016, Blast_Score=122, Evalue=2e-28,
Organism=Drosophila melanogaster, GI23397609, Length=249, Percent_Identity=30.9236947791165, Blast_Score=108, Evalue=4e-24,
Organism=Drosophila melanogaster, GI24643142, Length=249, Percent_Identity=31.3253012048193, Blast_Score=108, Evalue=5e-24,
Organism=Drosophila melanogaster, GI21355319, Length=248, Percent_Identity=26.2096774193548, Blast_Score=102, Evalue=4e-22,
Organism=Drosophila melanogaster, GI24640492, Length=193, Percent_Identity=30.0518134715026, Blast_Score=91, Evalue=5e-19,
Organism=Drosophila melanogaster, GI24644339, Length=250, Percent_Identity=29.2, Blast_Score=91, Evalue=6e-19,
Organism=Drosophila melanogaster, GI28571526, Length=258, Percent_Identity=30.6201550387597, Blast_Score=91, Evalue=7e-19,
Organism=Drosophila melanogaster, GI21357041, Length=249, Percent_Identity=28.9156626506024, Blast_Score=90, Evalue=1e-18,
Organism=Drosophila melanogaster, GI281364892, Length=196, Percent_Identity=31.1224489795918, Blast_Score=89, Evalue=2e-18,
Organism=Drosophila melanogaster, GI17737361, Length=251, Percent_Identity=27.4900398406374, Blast_Score=82, Evalue=5e-16,
Organism=Drosophila melanogaster, GI18859837, Length=227, Percent_Identity=27.7533039647577, Blast_Score=81, Evalue=7e-16,
Organism=Drosophila melanogaster, GI24644337, Length=250, Percent_Identity=29.6, Blast_Score=79, Evalue=3e-15,
Organism=Drosophila melanogaster, GI24581726, Length=196, Percent_Identity=27.5510204081633, Blast_Score=78, Evalue=5e-15,
Organism=Drosophila melanogaster, GI24584744, Length=195, Percent_Identity=28.2051282051282, Blast_Score=74, Evalue=7e-14,
Organism=Drosophila melanogaster, GI28574402, Length=195, Percent_Identity=28.2051282051282, Blast_Score=74, Evalue=8e-14,
Organism=Drosophila melanogaster, GI21358495, Length=198, Percent_Identity=25.7575757575758, Blast_Score=73, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24584748, Length=189, Percent_Identity=28.042328042328, Blast_Score=71, Evalue=7e-13,
Organism=Drosophila melanogaster, GI161076933, Length=189, Percent_Identity=28.042328042328, Blast_Score=71, Evalue=7e-13,
Organism=Drosophila melanogaster, GI24649181, Length=193, Percent_Identity=27.4611398963731, Blast_Score=66, Evalue=2e-11,
Organism=Drosophila melanogaster, GI21357673, Length=234, Percent_Identity=24.3589743589744, Blast_Score=64, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): IDNO_ECOL6 (P0A9Q0)

Other databases:

- EMBL:   AE014075
- RefSeq:   NP_757215.1
- ProteinModelPortal:   P0A9Q0
- SMR:   P0A9Q0
- EnsemblBacteria:   EBESCT00000040827
- GeneID:   1037302
- GenomeReviews:   AE014075_GR
- KEGG:   ecc:c5367
- GeneTree:   EBGT00050000009181
- HOGENOM:   HBG750976
- OMA:   RYMIKRQ
- ProtClustDB:   PRK08085
- BRENDA:   1.1.1.69
- GO:   GO:0005737
- GO:   GO:0005488
- InterPro:   IPR002198
- InterPro:   IPR002347
- InterPro:   IPR016040
- InterPro:   IPR020904
- Gene3D:   G3DSA:3.40.50.720
- PANTHER:   PTHR19410
- PRINTS:   PR00081
- PRINTS:   PR00080

Pfam domain/function: PF00106 adh_short

EC number: =1.1.1.69

Molecular weight: Translated: 27563; Mature: 27563

Theoretical pI: Translated: 6.31; Mature: 6.31

Prosite motif: PS00061 ADH_SHORT

Important sites: ACT_SITE 158-158 BINDING 145-145

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNDLFSLAGKNILITGSAQGIGFLLATGLGKYGAQIIINDITAERAELAVEKLHQEGIQA
CCCCHHHCCCEEEEEECCCCHHHHHHHCCCCCCCEEEEECHHHHHHHHHHHHHHHHHHHH
VAAPFNVTHKHEIDAAVEHIEKDIGPIDVLVNNAGIQRRHPFTEFPEQEWNDVIAVNQTA
HHCCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCHHCCCEEEECHHH
VFLVSQAVTRHMVERKAGKVINICSMQSELGRDTITPYAASKGAVKMLTRGMCVELARHN
HHHHHHHHHHHHHHHHCCCEEEEEHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHCC
IQVNGIAPGYFKTEMTKALVEDEAFTAWLCKRTPAARWGDPQELIGAAVFLSSKASDFVN
EEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCHHHHC
GHLLFVDGGMLVAV
CEEEEEECCEEEEC
>Mature Secondary Structure
MNDLFSLAGKNILITGSAQGIGFLLATGLGKYGAQIIINDITAERAELAVEKLHQEGIQA
CCCCHHHCCCEEEEEECCCCHHHHHHHCCCCCCCEEEEECHHHHHHHHHHHHHHHHHHHH
VAAPFNVTHKHEIDAAVEHIEKDIGPIDVLVNNAGIQRRHPFTEFPEQEWNDVIAVNQTA
HHCCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCHHCCCEEEECHHH
VFLVSQAVTRHMVERKAGKVINICSMQSELGRDTITPYAASKGAVKMLTRGMCVELARHN
HHHHHHHHHHHHHHHHCCCEEEEEHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHCC
IQVNGIAPGYFKTEMTKALVEDEAFTAWLCKRTPAARWGDPQELIGAAVFLSSKASDFVN
EEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCHHHHC
GHLLFVDGGMLVAV
CEEEEEECCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157