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The map label for this gene is idnD [H]

Identifier: 157163742

GI number: 157163742

Start: 4529504

End: 4530535

Strand: Reverse

Name: idnD [H]

Synonym: EcHS_A4523

Alternate gene names: 157163742

Gene position: 4530535-4529504 (Counterclockwise)

Preceding gene: 157163744

Following gene: 157163741

Centisome position: 97.57

GC content: 47.48

Gene sequence:

>1032_bases
ATGCAAGTGAAAACACAGTCCTGCGTTGTTGCGGGCAAGAAAACTGTTGCCGTTACCGAGCAGACGATAGATTGGAATAA
TAATGGAACATTAGTACAAATAACCCGAGGTGGAATTTGCGGTTCCGATTTACATTATTATCAGGAAGGAAAAGTAGGTA
ATTTCATGATAAAGGCACCGATGGTGTTAGGTCATGAAGTTATCGGTAAAGTTATTCATAGCGACTCATCAGAATTACAT
GAAGGGCAAACGGTAGCCATTAATCCGTCTAAACCGTGCGGTCACTGCAAATACTGCATTGAACATAACGAGAATCAGTG
TACAGAGATGCGTTTTTTTGGCAGTGCCATGTATTTCCCTCATGTTGATGGTGGTTTTACCCGTTATAAAATGGTCGAAA
CGTCGCAATGTGTCCCTTATCCGGCCAAAGCTGACGAAAAGGTTATGGCTTTTGCCGAACCTTTAGCCGTCGCGATTCAT
GCCGCACATCAGGCCGGCGAGTTACAGGGCAAGCGAGTATTTATTTCCGGTGTTGGACCCATTGGCTGCCTGATTGTCAG
TGCAGTGAAAACACTGGGGGCCGCGGAAATTGTCTGTGCTGATGTGAGTCCCCGTTCCCTTTCGCTGGGCAAAGAGATGG
GGGCGGATGTGCTCGTAAACCCACAAAACGACGACATGGATCACTGGAAAGCGGAAAAAGGCTATTTCGATGTCAGCTTT
GAAGTGTCCGGTCATCCTTCATCAGTGAATACCTGTCTGGAGGTCACTCGTGCACGCGGCGTAATGGTGCAGGTAGGTAT
GGGAGGCGCGATGGCAGAATTCCCAATGATGACGTTGATTGGTAAGGAGATTTCACTCAGAGGCTCTTTCCGTTTTACCA
GCGAATTTAATACCGCAGTGTCATGGCTGGCGAATGGCGTTATCAATCCACTGCCTTTACTGAGTGCTGAATATCCCTTC
ACTGACCTGGAAGAGGCGCTACGTTTCGCCGGTGATAAAACCCAGGCAGCAAAAGTCCAGCTTGTTTTCTAA

Upstream 100 bases:

>100_bases
TAGTAATGTAACAATTTTCTGACGTGATCTTCATCACAAATAATGACAATTAAAACCGCTTAAATGCTTCCGAGGTGTCT
ACCTGACCAGTGAGGAATTT

Downstream 100 bases:

>100_bases
GAGATAAATAAAGGAATAATACAATGAACGATCTATTTTCACTGGCAGGAAAAAATATCTTGATTACCGGTTCAGCACAG
GGCATTGGCTTTTTACTGGC

Product: L-idonate 5-dehydrogenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 343; Mature: 343

Protein sequence:

>343_residues
MQVKTQSCVVAGKKTVAVTEQTIDWNNNGTLVQITRGGICGSDLHYYQEGKVGNFMIKAPMVLGHEVIGKVIHSDSSELH
EGQTVAINPSKPCGHCKYCIEHNENQCTEMRFFGSAMYFPHVDGGFTRYKMVETSQCVPYPAKADEKVMAFAEPLAVAIH
AAHQAGELQGKRVFISGVGPIGCLIVSAVKTLGAAEIVCADVSPRSLSLGKEMGADVLVNPQNDDMDHWKAEKGYFDVSF
EVSGHPSSVNTCLEVTRARGVMVQVGMGGAMAEFPMMTLIGKEISLRGSFRFTSEFNTAVSWLANGVINPLPLLSAEYPF
TDLEEALRFAGDKTQAAKVQLVF

Sequences:

>Translated_343_residues
MQVKTQSCVVAGKKTVAVTEQTIDWNNNGTLVQITRGGICGSDLHYYQEGKVGNFMIKAPMVLGHEVIGKVIHSDSSELH
EGQTVAINPSKPCGHCKYCIEHNENQCTEMRFFGSAMYFPHVDGGFTRYKMVETSQCVPYPAKADEKVMAFAEPLAVAIH
AAHQAGELQGKRVFISGVGPIGCLIVSAVKTLGAAEIVCADVSPRSLSLGKEMGADVLVNPQNDDMDHWKAEKGYFDVSF
EVSGHPSSVNTCLEVTRARGVMVQVGMGGAMAEFPMMTLIGKEISLRGSFRFTSEFNTAVSWLANGVINPLPLLSAEYPF
TDLEEALRFAGDKTQAAKVQLVF
>Mature_343_residues
MQVKTQSCVVAGKKTVAVTEQTIDWNNNGTLVQITRGGICGSDLHYYQEGKVGNFMIKAPMVLGHEVIGKVIHSDSSELH
EGQTVAINPSKPCGHCKYCIEHNENQCTEMRFFGSAMYFPHVDGGFTRYKMVETSQCVPYPAKADEKVMAFAEPLAVAIH
AAHQAGELQGKRVFISGVGPIGCLIVSAVKTLGAAEIVCADVSPRSLSLGKEMGADVLVNPQNDDMDHWKAEKGYFDVSF
EVSGHPSSVNTCLEVTRARGVMVQVGMGGAMAEFPMMTLIGKEISLRGSFRFTSEFNTAVSWLANGVINPLPLLSAEYPF
TDLEEALRFAGDKTQAAKVQLVF

Specific function: Catalyzes the NADH/NADPH-dependent oxidation of L- idonate to 5-ketogluconate (5KG) [H]

COG id: COG1063

COG function: function code ER; Threonine dehydrogenase and related Zn-dependent dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the zinc-containing alcohol dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI156627571, Length=307, Percent_Identity=32.2475570032573, Blast_Score=168, Evalue=6e-42,
Organism=Escherichia coli, GI1790718, Length=343, Percent_Identity=99.7084548104956, Blast_Score=715, Evalue=0.0,
Organism=Escherichia coli, GI1788073, Length=308, Percent_Identity=29.2207792207792, Blast_Score=131, Evalue=5e-32,
Organism=Escherichia coli, GI1787863, Length=344, Percent_Identity=27.0348837209302, Blast_Score=131, Evalue=5e-32,
Organism=Escherichia coli, GI1788407, Length=336, Percent_Identity=28.5714285714286, Blast_Score=117, Evalue=2e-27,
Organism=Escherichia coli, GI1790045, Length=321, Percent_Identity=26.791277258567, Blast_Score=114, Evalue=7e-27,
Organism=Escherichia coli, GI226510992, Length=305, Percent_Identity=29.1803278688525, Blast_Score=113, Evalue=2e-26,
Organism=Escherichia coli, GI1786825, Length=370, Percent_Identity=25.4054054054054, Blast_Score=83, Evalue=3e-17,
Organism=Escherichia coli, GI1788075, Length=333, Percent_Identity=25.8258258258258, Blast_Score=83, Evalue=3e-17,
Organism=Escherichia coli, GI87081918, Length=331, Percent_Identity=27.190332326284, Blast_Score=82, Evalue=7e-17,
Organism=Escherichia coli, GI87082125, Length=256, Percent_Identity=26.953125, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1786552, Length=334, Percent_Identity=23.9520958083832, Blast_Score=62, Evalue=6e-11,
Organism=Caenorhabditis elegans, GI17562876, Length=324, Percent_Identity=34.2592592592593, Blast_Score=197, Evalue=9e-51,
Organism=Caenorhabditis elegans, GI17562878, Length=321, Percent_Identity=34.8909657320872, Blast_Score=171, Evalue=6e-43,
Organism=Saccharomyces cerevisiae, GI6319955, Length=312, Percent_Identity=32.3717948717949, Blast_Score=166, Evalue=5e-42,
Organism=Saccharomyces cerevisiae, GI6322619, Length=312, Percent_Identity=32.3717948717949, Blast_Score=166, Evalue=5e-42,
Organism=Saccharomyces cerevisiae, GI6323099, Length=340, Percent_Identity=30.8823529411765, Blast_Score=151, Evalue=2e-37,
Organism=Saccharomyces cerevisiae, GI6319258, Length=318, Percent_Identity=26.1006289308176, Blast_Score=106, Evalue=4e-24,
Organism=Saccharomyces cerevisiae, GI6319257, Length=314, Percent_Identity=25.796178343949, Blast_Score=84, Evalue=3e-17,
Organism=Drosophila melanogaster, GI17737897, Length=336, Percent_Identity=31.25, Blast_Score=171, Evalue=9e-43,
Organism=Drosophila melanogaster, GI17137530, Length=309, Percent_Identity=31.3915857605178, Blast_Score=155, Evalue=3e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013149
- InterPro:   IPR013154
- InterPro:   IPR002085
- InterPro:   IPR011032
- InterPro:   IPR016040 [H]

Pfam domain/function: PF08240 ADH_N; PF00107 ADH_zinc_N [H]

EC number: =1.1.1.264 [H]

Molecular weight: Translated: 37161; Mature: 37161

Theoretical pI: Translated: 6.28; Mature: 6.28

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.9 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
7.0 %Cys+Met (Translated Protein)
2.9 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
7.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQVKTQSCVVAGKKTVAVTEQTIDWNNNGTLVQITRGGICGSDLHYYQEGKVGNFMIKAP
CCCCCCEEEEECCEEEEEEEEEEEECCCCCEEEEECCCCCCCCCCEEECCCCCCEEEECC
MVLGHEVIGKVIHSDSSELHEGQTVAINPSKPCGHCKYCIEHNENQCTEMRFFGSAMYFP
HHHHHHHHHHHHCCCCHHHCCCCEEEECCCCCCCCHHHHHHCCCCCHHHHEEECCEEECC
HVDGGFTRYKMVETSQCVPYPAKADEKVMAFAEPLAVAIHAAHQAGELQGKRVFISGVGP
CCCCCCEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCH
IGCLIVSAVKTLGAAEIVCADVSPRSLSLGKEMGADVLVNPQNDDMDHWKAEKGYFDVSF
HHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHCCCCEEECCCCCCHHHHCCCCCEEEEEE
EVSGHPSSVNTCLEVTRARGVMVQVGMGGAMAEFPMMTLIGKEISLRGSFRFTSEFNTAV
EECCCCCHHHHHHHHHHCCCEEEEECCCCCHHHCCHHHHHCCEEEEEEEEEECCHHHHHH
SWLANGVINPLPLLSAEYPFTDLEEALRFAGDKTQAAKVQLVF
HHHHHCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCEEEEEEEC
>Mature Secondary Structure
MQVKTQSCVVAGKKTVAVTEQTIDWNNNGTLVQITRGGICGSDLHYYQEGKVGNFMIKAP
CCCCCCEEEEECCEEEEEEEEEEEECCCCCEEEEECCCCCCCCCCEEECCCCCCEEEECC
MVLGHEVIGKVIHSDSSELHEGQTVAINPSKPCGHCKYCIEHNENQCTEMRFFGSAMYFP
HHHHHHHHHHHHCCCCHHHCCCCEEEECCCCCCCCHHHHHHCCCCCHHHHEEECCEEECC
HVDGGFTRYKMVETSQCVPYPAKADEKVMAFAEPLAVAIHAAHQAGELQGKRVFISGVGP
CCCCCCEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCH
IGCLIVSAVKTLGAAEIVCADVSPRSLSLGKEMGADVLVNPQNDDMDHWKAEKGYFDVSF
HHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHCCCCEEECCCCCCHHHHCCCCCEEEEEE
EVSGHPSSVNTCLEVTRARGVMVQVGMGGAMAEFPMMTLIGKEISLRGSFRFTSEFNTAV
EECCCCCHHHHHHHHHHCCCEEEEECCCCCHHHCCHHHHHCCEEEEEEEEEECCHHHHHH
SWLANGVINPLPLLSAEYPFTDLEEALRFAGDKTQAAKVQLVF
HHHHHCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7610040; 9278503; 9658018 [H]