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The map label for this gene is idnT

Identifier: 157163740

GI number: 157163740

Start: 4527332

End: 4528651

Strand: Reverse

Name: idnT

Synonym: EcHS_A4521

Alternate gene names: 157163740

Gene position: 4528651-4527332 (Counterclockwise)

Preceding gene: 157163741

Following gene: 157163739

Centisome position: 97.53

GC content: 50.61

Gene sequence:

>1320_bases
ATGCCATTAATCATTATTGCGGCAGGCGTCGCGCTGCTTCTTATCCTGATGATCGGCTTTAAAGTTAACGGCTTTATTGC
CCTCGTTCTGGTAGCTGCCGTCGTCGGATTTGCCGAAGGGATGGATGCACAGGCCGTCCTGCACTCTATACAAAATGGTA
TCGGCAGCACGCTCGGCGGGCTGGCAATGATTCTCGGTTTCGGGGCCATGTTAGGCAAGCTGATTTCTGATACGGGTGCG
GCACAACGTATCGCCACTACGCTGATTGCTACTTTTGGTAAAAAACGCGTGCAATGGGCGCTAGTGATCACCGGTCTGGT
TGTGGGCCTCGCCATGTTTTTTGAAGTGGGTTTTGTCCTGCTGTTGCCGTTGGTATTTACCATCGTAGCATCATCAGGAT
TACCCCTGTTGTATGTTGGCGTACCAATGGTAGCAGCGCTCTCTGTAACCCACTGTTTTCTGCCGCCACATCCAGGGCCT
ACTGCCATCGCGACTATCTTTGAGGCTAATCTCGGAACGACTTTACTGTATGGATTTATCATTACCATTCCGACAGTTAT
TGTCGCAGGACCGCTGTTTTCTAAACTGCTAACTCGCTTTGAGAAAGCACCACCGGAAGGCTTATTTAATCCTCATCTGT
TTAGCGAAGAGGAGATGCCCTCCTTCTGGAACAGTATTTTCGCTGCCGTGATCCCGGTCATCCTGATGGCTATCGCCGCC
GTTTGTGAAATTACGTTACCGAAAACTAACACCGTGCGCCTCTTCTTTGAATTTGTCGGTAACCCTGCCGTTGCGCTGTT
TATTGCCATTGTTATTGCGATTTTCACACTGGGCCGACGTAATGGACGCACCATCGAGCAAATCATGGATATCATTGGGG
ATTCTATAGGCGCTATCGCGATGATTGTGTTTATTATCGCTGGCGGCGGCGCGTTTAAGCAGGTATTAGTAGATAGCGGT
GTCGGGCACTATATTTCACACTTAATGACCGGAACTACGCTTTCGCCGTTATTGATGTGCTGGACTGTTGCGGCGCTGTT
GCGTATCGCTCTGGGCTCTGCCACCGTCGCGGCCATTACCACCGCGGGTGTGGTGTTGCCGATTATCAACGTTACCCATG
CCGATCCCGCTTTAATGGTACTGGCAACCGGTGCGGGCAGCGTGATCGCGTCACACGTAAACGACCCTGGCTTCTGGCTA
TTTAAAGGGTATTTTAATCTGACGGTTGGTGAAACGTTGCGTACCTGGACGGTGATGGAAACCCTTATTTCTATTATGGG
TTTGCTGGGCGTGTTAGCCATTAACGCCGTATTGCACTGA

Upstream 100 bases:

>100_bases
TTGTTTGTTGATGGCGGCATGTTAGTGGCTGTTTAACACCTACTACCCTACGTGCAGCGCGAAAGCGCTGCTTTTCTGGC
ACTAATAAGAGATAAAGACT

Downstream 100 bases:

>100_bases
AAGAATAAAATATTGTGATGTAGTTTCGGGGGAAGATTGAAGATTCCCCCGACGTTGGGAGAAGTTATGCGCAATCACAG
AATTTCTTTACAGGATATCG

Product: Gnt-II system L-idonate transporter

Products: gluconate [Cytoplasm]; L-idonate [Cytoplasm]; Proton [Cytoplasm] [C]

Alternate protein names: L-Ido transporter; 5-keto-D-gluconate transporter

Number of amino acids: Translated: 439; Mature: 438

Protein sequence:

>439_residues
MPLIIIAAGVALLLILMIGFKVNGFIALVLVAAVVGFAEGMDAQAVLHSIQNGIGSTLGGLAMILGFGAMLGKLISDTGA
AQRIATTLIATFGKKRVQWALVITGLVVGLAMFFEVGFVLLLPLVFTIVASSGLPLLYVGVPMVAALSVTHCFLPPHPGP
TAIATIFEANLGTTLLYGFIITIPTVIVAGPLFSKLLTRFEKAPPEGLFNPHLFSEEEMPSFWNSIFAAVIPVILMAIAA
VCEITLPKTNTVRLFFEFVGNPAVALFIAIVIAIFTLGRRNGRTIEQIMDIIGDSIGAIAMIVFIIAGGGAFKQVLVDSG
VGHYISHLMTGTTLSPLLMCWTVAALLRIALGSATVAAITTAGVVLPIINVTHADPALMVLATGAGSVIASHVNDPGFWL
FKGYFNLTVGETLRTWTVMETLISIMGLLGVLAINAVLH

Sequences:

>Translated_439_residues
MPLIIIAAGVALLLILMIGFKVNGFIALVLVAAVVGFAEGMDAQAVLHSIQNGIGSTLGGLAMILGFGAMLGKLISDTGA
AQRIATTLIATFGKKRVQWALVITGLVVGLAMFFEVGFVLLLPLVFTIVASSGLPLLYVGVPMVAALSVTHCFLPPHPGP
TAIATIFEANLGTTLLYGFIITIPTVIVAGPLFSKLLTRFEKAPPEGLFNPHLFSEEEMPSFWNSIFAAVIPVILMAIAA
VCEITLPKTNTVRLFFEFVGNPAVALFIAIVIAIFTLGRRNGRTIEQIMDIIGDSIGAIAMIVFIIAGGGAFKQVLVDSG
VGHYISHLMTGTTLSPLLMCWTVAALLRIALGSATVAAITTAGVVLPIINVTHADPALMVLATGAGSVIASHVNDPGFWL
FKGYFNLTVGETLRTWTVMETLISIMGLLGVLAINAVLH
>Mature_438_residues
PLIIIAAGVALLLILMIGFKVNGFIALVLVAAVVGFAEGMDAQAVLHSIQNGIGSTLGGLAMILGFGAMLGKLISDTGAA
QRIATTLIATFGKKRVQWALVITGLVVGLAMFFEVGFVLLLPLVFTIVASSGLPLLYVGVPMVAALSVTHCFLPPHPGPT
AIATIFEANLGTTLLYGFIITIPTVIVAGPLFSKLLTRFEKAPPEGLFNPHLFSEEEMPSFWNSIFAAVIPVILMAIAAV
CEITLPKTNTVRLFFEFVGNPAVALFIAIVIAIFTLGRRNGRTIEQIMDIIGDSIGAIAMIVFIIAGGGAFKQVLVDSGV
GHYISHLMTGTTLSPLLMCWTVAALLRIALGSATVAAITTAGVVLPIINVTHADPALMVLATGAGSVIASHVNDPGFWLF
KGYFNLTVGETLRTWTVMETLISIMGLLGVLAINAVLH

Specific function: Transports L-idonate, D-gluconate and 5-keto-D- gluconate, from the periplasm across the inner membrane

COG id: COG2610

COG function: function code GE; H+/gluconate symporter and related permeases

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gntP permease family

Homologues:

Organism=Escherichia coli, GI1790716, Length=439, Percent_Identity=100, Blast_Score=853, Evalue=0.0,
Organism=Escherichia coli, GI48994935, Length=438, Percent_Identity=61.8721461187215, Blast_Score=532, Evalue=1e-152,
Organism=Escherichia coli, GI2367370, Length=449, Percent_Identity=48.5523385300668, Blast_Score=375, Evalue=1e-105,
Organism=Escherichia coli, GI1790776, Length=435, Percent_Identity=40.4597701149425, Blast_Score=336, Evalue=2e-93,
Organism=Escherichia coli, GI48994939, Length=439, Percent_Identity=36.9020501138952, Blast_Score=270, Evalue=2e-73,
Organism=Escherichia coli, GI1788707, Length=430, Percent_Identity=32.5581395348837, Blast_Score=246, Evalue=1e-66,
Organism=Escherichia coli, GI1789097, Length=440, Percent_Identity=33.1818181818182, Blast_Score=205, Evalue=5e-54,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): IDNT_ECOLI (P39344)

Other databases:

- EMBL:   U14003
- EMBL:   U00096
- EMBL:   AP009048
- PIR:   S56491
- RefSeq:   AP_004761.1
- RefSeq:   NP_418686.1
- ProteinModelPortal:   P39344
- DIP:   DIP-10014N
- MINT:   MINT-1218744
- STRING:   P39344
- EnsemblBacteria:   EBESCT00000002056
- EnsemblBacteria:   EBESCT00000018064
- GeneID:   948798
- GenomeReviews:   AP009048_GR
- GenomeReviews:   U00096_GR
- KEGG:   ecj:JW4222
- KEGG:   eco:b4265
- EchoBASE:   EB2428
- EcoGene:   EG12539
- eggNOG:   COG2610
- GeneTree:   EBGT00050000009137
- HOGENOM:   HBG649941
- OMA:   MCSHVND
- ProtClustDB:   CLSK2393443
- BioCyc:   EcoCyc:YJGT-MONOMER
- Genevestigator:   P39344
- InterPro:   IPR003474
- TIGRFAMs:   TIGR00791

Pfam domain/function: PF02447 GntP_permease

EC number: NA

Molecular weight: Translated: 46042; Mature: 45911

Theoretical pI: Translated: 7.02; Mature: 7.02

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x1904d35c)-; HASH(0x151ca490)-; HASH(0x1930eb4c)-; HASH(0x1976c760)-; HASH(0x1992ba4c)-; HASH(0x18d687d8)-; HASH(0x199190d0)-; HASH(0x195f8764)-; HASH(0x19919154)-; HASH(0x1986728c)-; HASH(0x18887000)-; HASH(0x199178e4)-; HASH(0x191d25dc)-; HASH(0x18ff7b48)-;

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHHHHHCH
>Mature Secondary Structure 
PLIIIAAGVALLLILMIGFKVNGFIALVLVAAVVGFAEGMDAQAVLHSIQNGIGSTLGG
CEEEEHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
LAMILGFGAMLGKLISDTGAAQRIATTLIATFGKKRVQWALVITGLVVGLAMFFEVGFVL
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LLPLVFTIVASSGLPLLYVGVPMVAALSVTHCFLPPHPGPTAIATIFEANLGTTLLYGFI
HHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCHHHHHHHHH
ITIPTVIVAGPLFSKLLTRFEKAPPEGLFNPHLFSEEEMPSFWNSIFAAVIPVILMAIAA
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
VCEITLPKTNTVRLFFEFVGNPAVALFIAIVIAIFTLGRRNGRTIEQIMDIIGDSIGAIA
HHHHCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCHHHHH
MIVFIIAGGGAFKQVLVDSGVGHYISHLMTGTTLSPLLMCWTVAALLRIALGSATVAAIT
HHHHHHCCCHHHHHHHHHCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHH
TAGVVLPIINVTHADPALMVLATGAGSVIASHVNDPGFWLFKGYFNLTVGETLRTWTVME
HHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHCCCCCEEEEEEEEEEEHHHHHHHHHHHH
TLISIMGLLGVLAINAVLH
HHHHHHHHHHHHHHHHHCH

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: gluconate [Periplasm]; Proton [Periplasm]; L-idonate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + gluconate [Periplasm] = Proton [Cytoplasm] + gluconate [Cytoplasm] Proton [Periplasm] + L-idonate [Periplasm] = L-idonate [Cytoplasm] + Proton [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7610040; 9278503; 9658018