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The map label for this gene is hflK

Identifier: 157163637

GI number: 157163637

Start: 4426774

End: 4428033

Strand: Direct

Name: hflK

Synonym: EcHS_A4416

Alternate gene names: 157163637

Gene position: 4426774-4428033 (Clockwise)

Preceding gene: 157163636

Following gene: 157163638

Centisome position: 95.33

GC content: 56.03

Gene sequence:

>1260_bases
ATGGCGTGGAATCAGCCCGGTAATAACGGACAAGACCGCGACCCGTGGGGAAGCAGCAAACCTGGCGGCAACTCTGAGGG
AAATGGAAACAAAGGCGGTCGCGATCAAGGGCCACCTGATTTAGATGATATCTTCCGCAAACTGAGCAAAAAGCTCGGTG
GTCTGGGCGGCGGTAAAGGCACCGGATCTGGCGGTGGCAGTTCATCGCAAGGCCCGCGCCCGCAGCTTGGCGGTCGTGTC
GTTACCATCGCAGCGGCAGCGATTGTCATTATCTGGGCGGCCAGTGGTTTCTATACCATTAAAGAAGCCGAACGCGGCGT
GGTAACACGCTTTGGTAAATTCAGCCATCTGGTTGAGCCGGGTCTGAACTGGAAACCGACGTTTATCGACGAAGTCAAAC
CGGTAAACGTGGAAGCCGTGCGTGAACTGGCCGCTTCCGGTGTGATGTTGACGTCGGACGAGAACGTAGTGCGCGTTGAG
ATGAACGTGCAGTACCGCGTTACCAATCCGGAAAAATATCTGTATAGCGTGACCAGCCCGGATGACAGTCTGCGTCAGGC
TACCGACAGCGCCCTGCGCGGGGTTATCGGTAAATACACCATGGACCGCATTCTGACGGAAGGTCGTACCGTGATTCGTA
GCGATACTCAGCGCGAACTGGAAGAGACTATCCGTCCGTATGACATGGGCATCACTCTGCTGGACGTCAACTTCCAGGCT
GCTCGTCCGCCGGAAGAAGTAAAAGCGGCGTTTGACGATGCGATTGCCGCGCGTGAAAACGAACAGCAATACATTCGTGA
AGCAGAAGCGTATACCAACGAAGTTCAGCCGCGTGCGAACGGTCAGGCGCAACGTATCCTCGAAGAGGCGCGTGCGTACA
AGGCCCAGACCATCCTGGAAGCTCAGGGTGAAGTGGCGCGCTTTGCTAAACTTCTGCCGGAATATAAAGCCGCGCCGGAA
ATTACTCGCGAGCGTCTGTATATCGAGACGATGGAAAAAGTGTTGGGTAACACCCGCAAAGTGCTGGTTAACGATAAAGG
TGGCAACCTGATGGTTCTGCCGTTAGACCAGATGCTGAAAGGTGGTAACGCCCCTGCGGCGAAGAGCGATAACGGTGCCA
GCAATCTGCTGCGTTTGCCGCCAGCCTCTTCCTCCACAACCAGTGGAGCAAGCAACACGTCGTCCACCAGTCAGGGCGAT
ATTATGGACCAACGCCGCGCCAACGCGCAGCGTAACGACTACCAGCGTCAGGGGGAATAA

Upstream 100 bases:

>100_bases
GATTACCTGATCTAACGGCGTAGCGTCTGAAGCGTGGAGTCATATCCTCTGGCGTCGAAAGACAACAGGGATCACCGCAT
AACAAATATGGAGCACAAAC

Downstream 100 bases:

>100_bases
CGATGCGTAAGTCAGTTATCGCGATTATCATCATCGTGCTGGTAGTGCTTTACATGTCTGTCTTTGTCGTCAAAGAAGGT
GAGCGCGGTATTACGCTGCG

Product: FtsH protease regulator HflK

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 419; Mature: 418

Protein sequence:

>419_residues
MAWNQPGNNGQDRDPWGSSKPGGNSEGNGNKGGRDQGPPDLDDIFRKLSKKLGGLGGGKGTGSGGGSSSQGPRPQLGGRV
VTIAAAAIVIIWAASGFYTIKEAERGVVTRFGKFSHLVEPGLNWKPTFIDEVKPVNVEAVRELAASGVMLTSDENVVRVE
MNVQYRVTNPEKYLYSVTSPDDSLRQATDSALRGVIGKYTMDRILTEGRTVIRSDTQRELEETIRPYDMGITLLDVNFQA
ARPPEEVKAAFDDAIAARENEQQYIREAEAYTNEVQPRANGQAQRILEEARAYKAQTILEAQGEVARFAKLLPEYKAAPE
ITRERLYIETMEKVLGNTRKVLVNDKGGNLMVLPLDQMLKGGNAPAAKSDNGASNLLRLPPASSSTTSGASNTSSTSQGD
IMDQRRANAQRNDYQRQGE

Sequences:

>Translated_419_residues
MAWNQPGNNGQDRDPWGSSKPGGNSEGNGNKGGRDQGPPDLDDIFRKLSKKLGGLGGGKGTGSGGGSSSQGPRPQLGGRV
VTIAAAAIVIIWAASGFYTIKEAERGVVTRFGKFSHLVEPGLNWKPTFIDEVKPVNVEAVRELAASGVMLTSDENVVRVE
MNVQYRVTNPEKYLYSVTSPDDSLRQATDSALRGVIGKYTMDRILTEGRTVIRSDTQRELEETIRPYDMGITLLDVNFQA
ARPPEEVKAAFDDAIAARENEQQYIREAEAYTNEVQPRANGQAQRILEEARAYKAQTILEAQGEVARFAKLLPEYKAAPE
ITRERLYIETMEKVLGNTRKVLVNDKGGNLMVLPLDQMLKGGNAPAAKSDNGASNLLRLPPASSSTTSGASNTSSTSQGD
IMDQRRANAQRNDYQRQGE
>Mature_418_residues
AWNQPGNNGQDRDPWGSSKPGGNSEGNGNKGGRDQGPPDLDDIFRKLSKKLGGLGGGKGTGSGGGSSSQGPRPQLGGRVV
TIAAAAIVIIWAASGFYTIKEAERGVVTRFGKFSHLVEPGLNWKPTFIDEVKPVNVEAVRELAASGVMLTSDENVVRVEM
NVQYRVTNPEKYLYSVTSPDDSLRQATDSALRGVIGKYTMDRILTEGRTVIRSDTQRELEETIRPYDMGITLLDVNFQAA
RPPEEVKAAFDDAIAARENEQQYIREAEAYTNEVQPRANGQAQRILEEARAYKAQTILEAQGEVARFAKLLPEYKAAPEI
TRERLYIETMEKVLGNTRKVLVNDKGGNLMVLPLDQMLKGGNAPAAKSDNGASNLLRLPPASSSTTSGASNTSSTSQGDI
MDQRRANAQRNDYQRQGE

Specific function: HflC and HflK govern the stability of phage lambda cII protein and have been proposed to encode or regulate a cII- specific protease

COG id: COG0330

COG function: function code O; Membrane protease subunits, stomatin/prohibitin homologs

Gene ontology:

Cell location: Membrane; Single-pass membrane protein (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the band 7/mec-2 family. HflK subfamily

Homologues:

Organism=Escherichia coli, GI1790616, Length=419, Percent_Identity=100, Blast_Score=853, Evalue=0.0,
Organism=Escherichia coli, GI1786697, Length=281, Percent_Identity=22.7758007117438, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HFLK_ECO57 (P0ABC8)

Other databases:

- EMBL:   AE005174
- EMBL:   BA000007
- PIR:   F86113
- PIR:   F91272
- RefSeq:   NP_290804.1
- RefSeq:   NP_313177.1
- ProteinModelPortal:   P0ABC8
- SMR:   P0ABC8
- EnsemblBacteria:   EBESCT00000026194
- EnsemblBacteria:   EBESCT00000060447
- GeneID:   914055
- GeneID:   959949
- GenomeReviews:   AE005174_GR
- GenomeReviews:   BA000007_GR
- KEGG:   ece:Z5781
- KEGG:   ecs:ECs5150
- GeneTree:   EBGT00050000010264
- HOGENOM:   HBG465357
- OMA:   WNEPGGN
- ProtClustDB:   PRK10930
- BioCyc:   ECOL83334:ECS5150-MONOMER
- InterPro:   IPR001107
- InterPro:   IPR010201
- InterPro:   IPR020980
- InterPro:   IPR001972
- PANTHER:   PTHR10264
- PRINTS:   PR00721
- SMART:   SM00244
- TIGRFAMs:   TIGR01933

Pfam domain/function: PF01145 Band_7; PF12221 HflK_N

EC number: NA

Molecular weight: Translated: 45545; Mature: 45414

Theoretical pI: Translated: 6.22; Mature: 6.22

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x193a7a9c)-;

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAWNQPGNNGQDRDPWGSSKPGGNSEGNGNKGGRDQGPPDLDDIFRKLSKKLGGLGGGKG
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCC
TGSGGGSSSQGPRPQLGGRVVTIAAAAIVIIWAASGFYTIKEAERGVVTRFGKFSHLVEP
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEHHHHCHHHHHHHHHHHHHCC
GLNWKPTFIDEVKPVNVEAVRELAASGVMLTSDENVVRVEMNVQYRVTNPEKYLYSVTSP
CCCCCCCHHCCCCCCCHHHHHHHHHCCEEEECCCCEEEEEEEEEEEEECHHHEEEEECCC
DDSLRQATDSALRGVIGKYTMDRILTEGRTVIRSDTQRELEETIRPYDMGITLLDVNFQA
CHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCHHHHHHHHHCCHHHCCEEEEEECCCC
ARPPEEVKAAFDDAIAARENEQQYIREAEAYTNEVQPRANGQAQRILEEARAYKAQTILE
CCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHH
AQGEVARFAKLLPEYKAAPEITRERLYIETMEKVLGNTRKVLVNDKGGNLMVLPLDQMLK
HCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCEEEEEHHHHHC
GGNAPAAKSDNGASNLLRLPPASSSTTSGASNTSSTSQGDIMDQRRANAQRNDYQRQGE
CCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCHHHHHHHCCH
>Mature Secondary Structure 
AWNQPGNNGQDRDPWGSSKPGGNSEGNGNKGGRDQGPPDLDDIFRKLSKKLGGLGGGKG
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCC
TGSGGGSSSQGPRPQLGGRVVTIAAAAIVIIWAASGFYTIKEAERGVVTRFGKFSHLVEP
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEHHHHCHHHHHHHHHHHHHCC
GLNWKPTFIDEVKPVNVEAVRELAASGVMLTSDENVVRVEMNVQYRVTNPEKYLYSVTSP
CCCCCCCHHCCCCCCCHHHHHHHHHCCEEEECCCCEEEEEEEEEEEEECHHHEEEEECCC
DDSLRQATDSALRGVIGKYTMDRILTEGRTVIRSDTQRELEETIRPYDMGITLLDVNFQA
CHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCHHHHHHHHHCCHHHCCEEEEEECCCC
ARPPEEVKAAFDDAIAARENEQQYIREAEAYTNEVQPRANGQAQRILEEARAYKAQTILE
CCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHH
AQGEVARFAKLLPEYKAAPEITRERLYIETMEKVLGNTRKVLVNDKGGNLMVLPLDQMLK
HCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCEEEEEHHHHHC
GGNAPAAKSDNGASNLLRLPPASSSTTSGASNTSSTSQGDIMDQRRANAQRNDYQRQGE
CCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCHHHHHHHCCH

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on peptide bonds (Peptidases) [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796