Definition Escherichia coli HS, complete genome.
Accession NC_009800
Length 4,643,538

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The map label for this gene is nrfG [H]

Identifier: 157163546

GI number: 157163546

Start: 4325309

End: 4325905

Strand: Direct

Name: nrfG [H]

Synonym: EcHS_A4321

Alternate gene names: 157163546

Gene position: 4325309-4325905 (Clockwise)

Preceding gene: 157163545

Following gene: 157163547

Centisome position: 93.15

GC content: 51.59

Gene sequence:

>597_bases
ATGAAAGAGCCCAAAATACCGGTGAAAATGCTGACAACACTCACGATTTTGATGGTATTTCTCTGTGTCGGCAGTTATCT
GTTAAGTCCAAAATGGCAGGCGGTACATGCGGAATATCAGCGTCAGCGCGATCCGCTACATCAGTTTGCCAGCCAGCAAA
CCCCGGAAGCGCAGCTTCAGGCATTGCAGGATAAAATCCGCGCTAATCCACAAAACAGCGAACAATGGGCGTTACTGGGC
GAGTACTATCTGTGGCAAAACGATTACAGCAATTCGCTGCTGGCGTACCGTCAGGCGTTGCAACTACGTGGTGAGAACGC
TGAACTGTATGCGGCGCTGGCGACGGTGCTTTATTACCAGGCCAGCCAGCATATGACCGCCCAGACTCGCGCAATGATCG
ACAAAGCCCTCGCGCTGGACTCTAACGAAATCACCGCTCTGATGCTGCTGGCTTCCGATGCGTTTATGCAGGCGAATTAC
GCGCAAGCCATCGAACTATGGCAAAAAGTGATGGATCTCAACTCACCCCGGATCAACCGGACACAGCTGGTCGAGTCGAT
TAATATGGCGAAATTGTTGCAGCGGAGATCAGATTAA

Upstream 100 bases:

>100_bases
ATAACCCACCGTTAACGGGTCAGACGCTGGTGTTGTGGGCGCTGCCAGTGGTGTTGTTACTGCTGATGGCACTGATCCTC
TGGCGAGTGAGGGCGAAGCG

Downstream 100 bases:

>100_bases
TCATCTGGTGATGAAGATTATGTTATTGAGTTGTATAAAAAATAATCGAATGAACATATGCCAAAAATAATCACCAATCA
GTATTATTGCAGATTAAACA

Product: formate-dependent nitrite reductase complex subunit NrfG

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 198; Mature: 198

Protein sequence:

>198_residues
MKEPKIPVKMLTTLTILMVFLCVGSYLLSPKWQAVHAEYQRQRDPLHQFASQQTPEAQLQALQDKIRANPQNSEQWALLG
EYYLWQNDYSNSLLAYRQALQLRGENAELYAALATVLYYQASQHMTAQTRAMIDKALALDSNEITALMLLASDAFMQANY
AQAIELWQKVMDLNSPRINRTQLVESINMAKLLQRRSD

Sequences:

>Translated_198_residues
MKEPKIPVKMLTTLTILMVFLCVGSYLLSPKWQAVHAEYQRQRDPLHQFASQQTPEAQLQALQDKIRANPQNSEQWALLG
EYYLWQNDYSNSLLAYRQALQLRGENAELYAALATVLYYQASQHMTAQTRAMIDKALALDSNEITALMLLASDAFMQANY
AQAIELWQKVMDLNSPRINRTQLVESINMAKLLQRRSD
>Mature_198_residues
MKEPKIPVKMLTTLTILMVFLCVGSYLLSPKWQAVHAEYQRQRDPLHQFASQQTPEAQLQALQDKIRANPQNSEQWALLG
EYYLWQNDYSNSLLAYRQALQLRGENAELYAALATVLYYQASQHMTAQTRAMIDKALALDSNEITALMLLASDAFMQANY
AQAIELWQKVMDLNSPRINRTQLVESINMAKLLQRRSD

Specific function: Required for formate-dependent nitrite reduction. Not required for the biosynthesis of any of the c-type cytochromes nor for the secretion of the periplasmic cytochromes [H]

COG id: COG4235

COG function: function code O; Cytochrome c biogenesis factor

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 TPR repeats [H]

Homologues:

Organism=Escherichia coli, GI1790513, Length=198, Percent_Identity=97.979797979798, Blast_Score=394, Evalue=1e-111,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013026
- InterPro:   IPR011990
- InterPro:   IPR019734 [H]

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 22754; Mature: 22754

Theoretical pI: Translated: 7.58; Mature: 7.58

Prosite motif: PS50005 TPR ; PS50293 TPR_REGION

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKEPKIPVKMLTTLTILMVFLCVGSYLLSPKWQAVHAEYQRQRDPLHQFASQQTPEAQLQ
CCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHH
ALQDKIRANPQNSEQWALLGEYYLWQNDYSNSLLAYRQALQLRGENAELYAALATVLYYQ
HHHHHHHCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
ASQHMTAQTRAMIDKALALDSNEITALMLLASDAFMQANYAQAIELWQKVMDLNSPRINR
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCH
TQLVESINMAKLLQRRSD
HHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKEPKIPVKMLTTLTILMVFLCVGSYLLSPKWQAVHAEYQRQRDPLHQFASQQTPEAQLQ
CCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHH
ALQDKIRANPQNSEQWALLGEYYLWQNDYSNSLLAYRQALQLRGENAELYAALATVLYYQ
HHHHHHHCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
ASQHMTAQTRAMIDKALALDSNEITALMLLASDAFMQANYAQAIELWQKVMDLNSPRINR
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCH
TQLVESINMAKLLQRRSD
HHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]