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The map label for this gene is gltP [H]

Identifier: 157163547

GI number: 157163547

Start: 4326247

End: 4327560

Strand: Direct

Name: gltP [H]

Synonym: EcHS_A4322

Alternate gene names: 157163547

Gene position: 4326247-4327560 (Clockwise)

Preceding gene: 157163546

Following gene: 157163555

Centisome position: 93.17

GC content: 53.81

Gene sequence:

>1314_bases
ATGAAAAGTATAAAATTCAGCCTGGCCTGGCAGATTCTGTTTGCTATGGTGCTGGGCATTCTCCTGGGAAGCTACCTGCA
CTACCATAGCGACAGCCGCGACTGGCTGGTCGTCAATTTGCTCTCTCCGGCGGGTGATATCTTCATCCATCTGATCAAAA
TGATTGTTGTGCCGATTGTGATCTCCACGCTGGTGGTGGGTATCGCGGGTGTTGGTGATGCCAAACAGCTCGGGCGTATT
GGCGCGAAAACCATTATTTACTTCGAGGTGATCACCACCGTCGCCATCATTTTGGGGATCACTCTGGCGAACGTCTTCCA
GCCCGGTGCCGGGGTGGATATGTCGCAGCTGGCGACCGTCGATATCTCGAAATATCAGAGCACTACGGAAGCGGTACAAA
GCAGTTCCCACGGCATTATGGGCACGATTTTGTCGCTGGTGCCGACGAACATTGTGGCGTCGATGGCGAAAGGCGAAATG
CTGCCGATCATCTTCTTCTCGGTGCTGTTTGGTCTGGGGCTTTCCTCCCTGCCCGCGACGCATCGTGAACCGCTGGTGAC
CGTGTTCCGCTCCATCTCTGAAACCATGTTTAAAGTGACTCACATGGTGATGCGTTATGCACCGGTGGGTGTGTTTGCGC
TGATTGCGGTGACGGTGGCTAACTTTGGTTTCTCTTCTCTGTGGCCACTGGCGAAACTGGTGCTGCTGGTGCATTTCGCC
ATTCTGTTCTTCGCGCTGGTAGTGCTGGGAATTGTGGCGCGCCTGTGCGGGTTAAGCGTCTGGATCCTGATTCGTATTCT
GAAAGATGAGCTGATTCTGGCGTACTCCACTGCCAGCTCTGAAAGCGTACTGCCGCGAATTATTGAGAAGATGGAAGCCT
ACGGAGCACCGGCGTCGATCACCAGCTTCGTGGTGCCGACCGGTTACTCTTTTAACCTTGATGGTTCGACGCTGTATCAA
AGTATTGCCGCTATTTTCATCGCTCAGCTGTATGGCATTGACCTGTCCATCTGGCAGGAAATCATTCTGGTGCTGACGCT
GATGGTGACCTCGAAAGGGATTGCTGGCGTGCCGGGCGTGTCGTTTGTGGTGCTGCTGGCAACGCTGGGTAGCGTAGGTA
TTCCGCTGGAAGGCCTGGCGTTTATTGCTGGTGTTGACCGTATCCTCGACATGGCGCGTACTGCGCTGAACGTGGTGGGT
AATGCGCTGGCGGTGCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGCAAAAAGGCGCTGGCTTATGAGCGTGAAGT
GCTGGGCAAATTTGATAAAACTGCGGATCAATAA

Upstream 100 bases:

>100_bases
AAAATCTACAAACCAACGCAACACAATTCATGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGAACGGGGAACGGCGCT
CCATTGAGGAAGTTATTCTG

Downstream 100 bases:

>100_bases
TTGAAGATTGCCGGGGTTATCCACCCGGCAATGTGTGGATGCCTGATGCGACGCTGGTGCGTCTTATCAGGCCTACCTTA
ACCGTTGGTTAATTTTTCAA

Product: glutamate/aspartate:proton symporter

Products: L-aspartate [Cytoplasm]; Proton [Cytoplasm]; L-glutamate [Cytoplasm] [C]

Alternate protein names: Glutamate-aspartate carrier protein [H]

Number of amino acids: Translated: 437; Mature: 437

Protein sequence:

>437_residues
MKSIKFSLAWQILFAMVLGILLGSYLHYHSDSRDWLVVNLLSPAGDIFIHLIKMIVVPIVISTLVVGIAGVGDAKQLGRI
GAKTIIYFEVITTVAIILGITLANVFQPGAGVDMSQLATVDISKYQSTTEAVQSSSHGIMGTILSLVPTNIVASMAKGEM
LPIIFFSVLFGLGLSSLPATHREPLVTVFRSISETMFKVTHMVMRYAPVGVFALIAVTVANFGFSSLWPLAKLVLLVHFA
ILFFALVVLGIVARLCGLSVWILIRILKDELILAYSTASSESVLPRIIEKMEAYGAPASITSFVVPTGYSFNLDGSTLYQ
SIAAIFIAQLYGIDLSIWQEIILVLTLMVTSKGIAGVPGVSFVVLLATLGSVGIPLEGLAFIAGVDRILDMARTALNVVG
NALAVLVIAKWEHKFDRKKALAYEREVLGKFDKTADQ

Sequences:

>Translated_437_residues
MKSIKFSLAWQILFAMVLGILLGSYLHYHSDSRDWLVVNLLSPAGDIFIHLIKMIVVPIVISTLVVGIAGVGDAKQLGRI
GAKTIIYFEVITTVAIILGITLANVFQPGAGVDMSQLATVDISKYQSTTEAVQSSSHGIMGTILSLVPTNIVASMAKGEM
LPIIFFSVLFGLGLSSLPATHREPLVTVFRSISETMFKVTHMVMRYAPVGVFALIAVTVANFGFSSLWPLAKLVLLVHFA
ILFFALVVLGIVARLCGLSVWILIRILKDELILAYSTASSESVLPRIIEKMEAYGAPASITSFVVPTGYSFNLDGSTLYQ
SIAAIFIAQLYGIDLSIWQEIILVLTLMVTSKGIAGVPGVSFVVLLATLGSVGIPLEGLAFIAGVDRILDMARTALNVVG
NALAVLVIAKWEHKFDRKKALAYEREVLGKFDKTADQ
>Mature_437_residues
MKSIKFSLAWQILFAMVLGILLGSYLHYHSDSRDWLVVNLLSPAGDIFIHLIKMIVVPIVISTLVVGIAGVGDAKQLGRI
GAKTIIYFEVITTVAIILGITLANVFQPGAGVDMSQLATVDISKYQSTTEAVQSSSHGIMGTILSLVPTNIVASMAKGEM
LPIIFFSVLFGLGLSSLPATHREPLVTVFRSISETMFKVTHMVMRYAPVGVFALIAVTVANFGFSSLWPLAKLVLLVHFA
ILFFALVVLGIVARLCGLSVWILIRILKDELILAYSTASSESVLPRIIEKMEAYGAPASITSFVVPTGYSFNLDGSTLYQ
SIAAIFIAQLYGIDLSIWQEIILVLTLMVTSKGIAGVPGVSFVVLLATLGSVGIPLEGLAFIAGVDRILDMARTALNVVG
NALAVLVIAKWEHKFDRKKALAYEREVLGKFDKTADQ

Specific function: This carrier protein is part of the Na(+)-independent, binding-protein-independent glutamate-aspartate transport system [H]

COG id: COG1301

COG function: function code C; Na+/H+-dicarboxylate symporters

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sodium:dicarboxylate (SDF) symporter (TC 2.A.23) family [H]

Homologues:

Organism=Homo sapiens, GI40254478, Length=452, Percent_Identity=27.4336283185841, Blast_Score=143, Evalue=4e-34,
Organism=Homo sapiens, GI169790839, Length=469, Percent_Identity=27.7185501066098, Blast_Score=141, Evalue=1e-33,
Organism=Homo sapiens, GI66773030, Length=470, Percent_Identity=28.5106382978723, Blast_Score=140, Evalue=2e-33,
Organism=Homo sapiens, GI194239697, Length=470, Percent_Identity=26.3829787234043, Blast_Score=135, Evalue=7e-32,
Organism=Homo sapiens, GI21314632, Length=439, Percent_Identity=26.6514806378132, Blast_Score=125, Evalue=6e-29,
Organism=Homo sapiens, GI4827012, Length=423, Percent_Identity=28.8416075650118, Blast_Score=118, Evalue=9e-27,
Organism=Homo sapiens, GI5032093, Length=409, Percent_Identity=27.6283618581907, Blast_Score=114, Evalue=2e-25,
Organism=Homo sapiens, GI262359914, Length=413, Percent_Identity=26.1501210653753, Blast_Score=105, Evalue=1e-22,
Organism=Homo sapiens, GI223468566, Length=278, Percent_Identity=29.136690647482, Blast_Score=103, Evalue=3e-22,
Organism=Homo sapiens, GI223468564, Length=278, Percent_Identity=29.136690647482, Blast_Score=103, Evalue=3e-22,
Organism=Homo sapiens, GI301601644, Length=193, Percent_Identity=30.5699481865285, Blast_Score=88, Evalue=2e-17,
Organism=Escherichia coli, GI1790514, Length=437, Percent_Identity=99.5423340961098, Blast_Score=862, Evalue=0.0,
Organism=Escherichia coli, GI1789947, Length=421, Percent_Identity=39.9049881235154, Blast_Score=322, Evalue=4e-89,
Organism=Escherichia coli, GI1788024, Length=420, Percent_Identity=24.7619047619048, Blast_Score=106, Evalue=4e-24,
Organism=Escherichia coli, GI1789473, Length=388, Percent_Identity=23.1958762886598, Blast_Score=73, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI17537407, Length=419, Percent_Identity=27.9236276849642, Blast_Score=156, Evalue=2e-38,
Organism=Caenorhabditis elegans, GI71983099, Length=422, Percent_Identity=27.4881516587678, Blast_Score=154, Evalue=1e-37,
Organism=Caenorhabditis elegans, GI71983106, Length=422, Percent_Identity=27.4881516587678, Blast_Score=153, Evalue=1e-37,
Organism=Caenorhabditis elegans, GI71996953, Length=455, Percent_Identity=26.8131868131868, Blast_Score=147, Evalue=1e-35,
Organism=Caenorhabditis elegans, GI193206505, Length=457, Percent_Identity=28.8840262582057, Blast_Score=144, Evalue=7e-35,
Organism=Caenorhabditis elegans, GI193206654, Length=413, Percent_Identity=26.634382566586, Blast_Score=121, Evalue=9e-28,
Organism=Caenorhabditis elegans, GI17541374, Length=429, Percent_Identity=25.8741258741259, Blast_Score=105, Evalue=4e-23,
Organism=Drosophila melanogaster, GI24583025, Length=463, Percent_Identity=26.9978401727862, Blast_Score=132, Evalue=6e-31,
Organism=Drosophila melanogaster, GI17137668, Length=463, Percent_Identity=26.9978401727862, Blast_Score=132, Evalue=6e-31,
Organism=Drosophila melanogaster, GI24583023, Length=463, Percent_Identity=26.9978401727862, Blast_Score=132, Evalue=6e-31,
Organism=Drosophila melanogaster, GI281360481, Length=459, Percent_Identity=25.7080610021786, Blast_Score=82, Evalue=8e-16,
Organism=Drosophila melanogaster, GI281360483, Length=469, Percent_Identity=25.5863539445629, Blast_Score=82, Evalue=9e-16,
Organism=Drosophila melanogaster, GI17137666, Length=469, Percent_Identity=25.5863539445629, Blast_Score=82, Evalue=9e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001991
- InterPro:   IPR018107 [H]

Pfam domain/function: PF00375 SDF [H]

EC number: NA

Molecular weight: Translated: 47105; Mature: 47105

Theoretical pI: Translated: 8.82; Mature: 8.82

Prosite motif: PS00713 NA_DICARBOXYL_SYMP_1 ; PS00714 NA_DICARBOXYL_SYMP_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHCCCCC
>Mature Secondary Structure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HHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: L-aspartate [Periplasm]; Proton [Periplasm]; L-glutamate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + L-aspartate [Periplasm] = Proton [Cytoplasm] + L-aspartate [Cytoplasm] Proton [Periplasm] + L-glutamate [Periplasm] = Proton [Cytoplasm] + L-glutamate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1971622; 1551855; 8265357; 9278503 [H]