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The map label for this gene is nrfF [H]

Identifier: 157163545

GI number: 157163545

Start: 4324929

End: 4325312

Strand: Direct

Name: nrfF [H]

Synonym: EcHS_A4320

Alternate gene names: 157163545

Gene position: 4324929-4325312 (Clockwise)

Preceding gene: 157163544

Following gene: 157163546

Centisome position: 93.14

GC content: 52.6

Gene sequence:

>384_bases
ATGAATAAAGGACTTCTCACGCTACTGCTGTTATTTACCTGTTTTGCGCGCGCTCAGGTCGTAGACACCTGGCAATTCGC
CAATCCGCAACAACAGCAACAGGCGTTAAATATTGCCAGCCAGTTACGTTGTCCGCAGTGCCAGAATCAAAACTTACTGG
AATCCAACGCGCCGGTGGCAGTCAGTATGCGCCATCAGGTTTACAGCATGGTGGCGGAGGGGAAGAACGAAGTCGAAATC
ATTGGCTGGATGACCGAACGCTACGGAGATTTTGTTCGCTATAACCCACCGTTAACGGGTCAGACGCTGGTGTTGTGGGC
GCTGCCAGTGGTGTTGTTACTGCTGATGGCACTGATCCTCTGGCGAGTGAGGGCGAAGCGATGA

Upstream 100 bases:

>100_bases
TTTCGCTTGTATGTACAAAGCGGTGTGCGCTGGATCTGGGGGGGAGGATTGTTGATGATTGCGGGCGCATTGTTAAGCGG
ATGGCGGGGGAGGAAGCGCG

Downstream 100 bases:

>100_bases
AAGAGCCCAAAATACCGGTGAAAATGCTGACAACACTCACGATTTTGATGGTATTTCTCTGTGTCGGCAGTTATCTGTTA
AGTCCAAAATGGCAGGCGGT

Product: formate-dependent nitrite reductase complex subunit NrfF

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 127; Mature: 127

Protein sequence:

>127_residues
MNKGLLTLLLLFTCFARAQVVDTWQFANPQQQQQALNIASQLRCPQCQNQNLLESNAPVAVSMRHQVYSMVAEGKNEVEI
IGWMTERYGDFVRYNPPLTGQTLVLWALPVVLLLLMALILWRVRAKR

Sequences:

>Translated_127_residues
MNKGLLTLLLLFTCFARAQVVDTWQFANPQQQQQALNIASQLRCPQCQNQNLLESNAPVAVSMRHQVYSMVAEGKNEVEI
IGWMTERYGDFVRYNPPLTGQTLVLWALPVVLLLLMALILWRVRAKR
>Mature_127_residues
MNKGLLTLLLLFTCFARAQVVDTWQFANPQQQQQALNIASQLRCPQCQNQNLLESNAPVAVSMRHQVYSMVAEGKNEVEI
IGWMTERYGDFVRYNPPLTGQTLVLWALPVVLLLLMALILWRVRAKR

Specific function: Not required for the biosynthesis of any of the c-type cytochromes. Possible subunit of a heme lyase [H]

COG id: COG3088

COG function: function code O; Uncharacterized protein involved in biosynthesis of c-type cytochromes

Gene ontology:

Cell location: Periplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CcmH/CycL/Ccl2/NrfF family [H]

Homologues:

Organism=Escherichia coli, GI1790512, Length=127, Percent_Identity=99.2125984251969, Blast_Score=258, Evalue=1e-70,
Organism=Escherichia coli, GI1788522, Length=117, Percent_Identity=39.3162393162393, Blast_Score=107, Evalue=3e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005616
- InterPro:   IPR017565 [H]

Pfam domain/function: PF03918 CcmH [H]

EC number: NA

Molecular weight: Translated: 14542; Mature: 14542

Theoretical pI: Translated: 9.33; Mature: 9.33

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
6.3 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
6.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKGLLTLLLLFTCFARAQVVDTWQFANPQQQQQALNIASQLRCPQCQNQNLLESNAPVA
CCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEE
VSMRHQVYSMVAEGKNEVEIIGWMTERYGDFVRYNPPLTGQTLVLWALPVVLLLLMALIL
EHHHHHHHHHHHCCCCCEEEEEHHHHHHCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHH
WRVRAKR
HHHHCCC
>Mature Secondary Structure
MNKGLLTLLLLFTCFARAQVVDTWQFANPQQQQQALNIASQLRCPQCQNQNLLESNAPVA
CCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEE
VSMRHQVYSMVAEGKNEVEIIGWMTERYGDFVRYNPPLTGQTLVLWALPVVLLLLMALIL
EHHHHHHHHHHHCCCCCEEEEEHHHHHHCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHH
WRVRAKR
HHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]