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The map label for this gene is nrfE [H]

Identifier: 157163544

GI number: 157163544

Start: 4323278

End: 4324936

Strand: Direct

Name: nrfE [H]

Synonym: EcHS_A4319

Alternate gene names: 157163544

Gene position: 4323278-4324936 (Clockwise)

Preceding gene: 157163543

Following gene: 157163545

Centisome position: 93.1

GC content: 57.02

Gene sequence:

>1659_bases
GTGTTGACCCCGTTGACGGCCTTTGCGGGAGTGCGGTTGCGCTGGCCTGCCATGATGCGACTCACTTGCATCGGCATTCT
GGCGCAGTTCGCGCTCCTGCTGCTCGCCTTTGGCGTACTGACGTATTGTTTTCTCATCAGCGATTTCTCGGTCATTTATG
TCGCCCAACATAGCTACAGCCTGCTGTCGTGGGAACTCAAACTGGCGGCGGTGTGGGGCGGCCATGAAGGTTCGCTGCTG
CTTTGGGTGCTGCTGCTTTCCGCCTGGAGCGCGCTGTTTGCCTGGCATTATCGGCAGCAAACCGATCCGCTATTTCCGCT
GACGCTAGCCGTTTTATCTCTCATGCTCGCCGCACTGCTACTGTTTGTAGTGCTGTGGTCCGATCCCTTCGTGCGGATAT
TTCCACCAGCAATCGAAGGCCGCGATCTCAATCCGATGCTGCAACATCCCGGTCTTATCTTTCATCCACCGCTGCTTTAC
CTTGGCTATGGCGGTTTGATGGTAGCGGCGAGCGTGGCGCTGGCGAGTTTACTGCGCGGCGAGTTTGATGGTGCCTGCGC
CAGAATTTGTTGGCGCTGGGCACTACCTGGCTGGAGCGCATTAACGGCGGGGATCATCCTCGGTTCTTGGTGGGCCTATT
GCGAACTGGGCTGGGGCGGCTGGTGGTTCTGGGATCCGGTGGAAAACGCCTCTTTATTACCCTGGCTTTCTGCCACTGCG
CTGCTGCACAGTTTGTCCCTGACACGCCAGCGGGAGATTTTCCGCCACTGGTCGCTGTTGCTGGCGATAGTAACTCTGAT
GCTGTCGCTACTGGGTACCTTAATTGTCCGTTCTGGCATTCTGGTTTCGGTTCATGCGTTCGCACTGGATAACGTCCGCG
CCGTGCCGTTGTTCAGCCTGTTTGCACTGATTAGCCTTGCGTCTCTGGCTCTGTATGGCTGGCGAGCGCGGGACGGTGGT
GCGGTGGTGCGTTTTTCGGGGTTATCGCGGGAAATGTTAATCCTCGCTACGCTGTTGCTGTTTTGCGCAGTGCTACTGAT
CGTGCTGGTGGGAACGCTTTATCCGATGATTTACGGCCTGCTGGGCTGGGGACGCCTCTCCGTTGGCGCGCCGTATTTTA
ACCGCGCGACGTTACCGTTTGGCCTGTTGATGCTGGTGGTGATTGTGCTGGCGACGTTTGTCTCTGGCAAACGCGTGCAG
CTTCCGGCGCTGGTAGCTCATGCGGGCGTGCTGTTATTTGCCGCGGGGATCGTGGTTTCCAGCGTCAGCCGTCAGGAGAT
CAGCCTGAATTTACAGCCGGGTCAGCAGGTGACGCTGGCAGGATACACCTTCCGTTTTGAGCGCCTCGATCTGCAAGCCA
AAGGCAATTACACCAGCGAAAAAGCGATAGTGGCACTGTTTGACCATCAGCAACGCATTGGTGAACTGATGCCGGAGCGG
CGTTTTTACGAAGCACGTCGTCAGCAAATGATGGAACCGTCAATTCGCTGGAACGGCATCCATGACTGGTATGCGGTCAT
GGGTGAAAAAACCGGAGCGGATCGTTACGCTTTTCGCTTGTATGTACAAAGCGGTGTGCGCTGGATCTGGGGGGGAGGAT
TGTTGATGATTGCGGGCGCATTGTTAAGCGGATGGCGGGGGAGGAAGCGCGATGAATAA

Upstream 100 bases:

>100_bases
GGGCAGTTAACGGTGGCGTAAGCCAGAAAAGAGGTGGTTTCTGGACGTATTCCTTCCTGAAGTCGGTTTTCTGGCGTTGT
TGTTAAGTCTCGGGGTCAAC

Downstream 100 bases:

>100_bases
AGGACTTCTCACGCTACTGCTGTTATTTACCTGTTTTGCGCGCGCTCAGGTCGTAGACACCTGGCAATTCGCCAATCCGC
AACAACAGCAACAGGCGTTA

Product: heme lyase subunit NrfE

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 552; Mature: 552

Protein sequence:

>552_residues
MLTPLTAFAGVRLRWPAMMRLTCIGILAQFALLLLAFGVLTYCFLISDFSVIYVAQHSYSLLSWELKLAAVWGGHEGSLL
LWVLLLSAWSALFAWHYRQQTDPLFPLTLAVLSLMLAALLLFVVLWSDPFVRIFPPAIEGRDLNPMLQHPGLIFHPPLLY
LGYGGLMVAASVALASLLRGEFDGACARICWRWALPGWSALTAGIILGSWWAYCELGWGGWWFWDPVENASLLPWLSATA
LLHSLSLTRQREIFRHWSLLLAIVTLMLSLLGTLIVRSGILVSVHAFALDNVRAVPLFSLFALISLASLALYGWRARDGG
AVVRFSGLSREMLILATLLLFCAVLLIVLVGTLYPMIYGLLGWGRLSVGAPYFNRATLPFGLLMLVVIVLATFVSGKRVQ
LPALVAHAGVLLFAAGIVVSSVSRQEISLNLQPGQQVTLAGYTFRFERLDLQAKGNYTSEKAIVALFDHQQRIGELMPER
RFYEARRQQMMEPSIRWNGIHDWYAVMGEKTGADRYAFRLYVQSGVRWIWGGGLLMIAGALLSGWRGRKRDE

Sequences:

>Translated_552_residues
MLTPLTAFAGVRLRWPAMMRLTCIGILAQFALLLLAFGVLTYCFLISDFSVIYVAQHSYSLLSWELKLAAVWGGHEGSLL
LWVLLLSAWSALFAWHYRQQTDPLFPLTLAVLSLMLAALLLFVVLWSDPFVRIFPPAIEGRDLNPMLQHPGLIFHPPLLY
LGYGGLMVAASVALASLLRGEFDGACARICWRWALPGWSALTAGIILGSWWAYCELGWGGWWFWDPVENASLLPWLSATA
LLHSLSLTRQREIFRHWSLLLAIVTLMLSLLGTLIVRSGILVSVHAFALDNVRAVPLFSLFALISLASLALYGWRARDGG
AVVRFSGLSREMLILATLLLFCAVLLIVLVGTLYPMIYGLLGWGRLSVGAPYFNRATLPFGLLMLVVIVLATFVSGKRVQ
LPALVAHAGVLLFAAGIVVSSVSRQEISLNLQPGQQVTLAGYTFRFERLDLQAKGNYTSEKAIVALFDHQQRIGELMPER
RFYEARRQQMMEPSIRWNGIHDWYAVMGEKTGADRYAFRLYVQSGVRWIWGGGLLMIAGALLSGWRGRKRDE
>Mature_552_residues
MLTPLTAFAGVRLRWPAMMRLTCIGILAQFALLLLAFGVLTYCFLISDFSVIYVAQHSYSLLSWELKLAAVWGGHEGSLL
LWVLLLSAWSALFAWHYRQQTDPLFPLTLAVLSLMLAALLLFVVLWSDPFVRIFPPAIEGRDLNPMLQHPGLIFHPPLLY
LGYGGLMVAASVALASLLRGEFDGACARICWRWALPGWSALTAGIILGSWWAYCELGWGGWWFWDPVENASLLPWLSATA
LLHSLSLTRQREIFRHWSLLLAIVTLMLSLLGTLIVRSGILVSVHAFALDNVRAVPLFSLFALISLASLALYGWRARDGG
AVVRFSGLSREMLILATLLLFCAVLLIVLVGTLYPMIYGLLGWGRLSVGAPYFNRATLPFGLLMLVVIVLATFVSGKRVQ
LPALVAHAGVLLFAAGIVVSSVSRQEISLNLQPGQQVTLAGYTFRFERLDLQAKGNYTSEKAIVALFDHQQRIGELMPER
RFYEARRQQMMEPSIRWNGIHDWYAVMGEKTGADRYAFRLYVQSGVRWIWGGGLLMIAGALLSGWRGRKRDE

Specific function: May be required for the biogenesis of c-type cytochromes. Possible subunit of a heme lyase [H]

COG id: COG1138

COG function: function code O; Cytochrome c biogenesis factor

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ccmF/cycK/ccl1/nrfE/ccsA family [H]

Homologues:

Organism=Escherichia coli, GI1790511, Length=552, Percent_Identity=98.1884057971015, Blast_Score=1065, Evalue=0.0,
Organism=Escherichia coli, GI1788524, Length=584, Percent_Identity=38.6986301369863, Blast_Score=338, Evalue=6e-94,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002541
- InterPro:   IPR003567
- InterPro:   IPR003568
- InterPro:   IPR003570 [H]

Pfam domain/function: PF01578 Cytochrom_C_asm [H]

EC number: NA

Molecular weight: Translated: 61541; Mature: 61541

Theoretical pI: Translated: 9.71; Mature: 9.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTPLTAFAGVRLRWPAMMRLTCIGILAQFALLLLAFGVLTYCFLISDFSVIYVAQHSYS
CCCCCHHHCCCEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCE
LLSWELKLAAVWGGHEGSLLLWVLLLSAWSALFAWHYRQQTDPLFPLTLAVLSLMLAALL
EEEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHH
LFVVLWSDPFVRIFPPAIEGRDLNPMLQHPGLIFHPPLLYLGYGGLMVAASVALASLLRG
HHHHHHCCCCEEEECCCCCCCCCCHHHHCCCEEECCCHHHHCCHHHHHHHHHHHHHHHHC
EFDGACARICWRWALPGWSALTAGIILGSWWAYCELGWGGWWFWDPVENASLLPWLSATA
CCCHHHHHHHHHHCCCCHHHHHHHHHHHCHHHHEEECCCCEEEECCCCCCCCCHHHHHHH
LLHSLSLTRQREIFRHWSLLLAIVTLMLSLLGTLIVRSGILVSVHAFALDNVRAVPLFSL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHEEEHHHHHCCCCHHHHHHH
FALISLASLALYGWRARDGGAVVRFSGLSREMLILATLLLFCAVLLIVLVGTLYPMIYGL
HHHHHHHHHHHHCCEECCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LGWGRLSVGAPYFNRATLPFGLLMLVVIVLATFVSGKRVQLPALVAHAGVLLFAAGIVVS
HCCCHHCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHH
SVSRQEISLNLQPGQQVTLAGYTFRFERLDLQAKGNYTSEKAIVALFDHQQRIGELMPER
CCCHHHEEEEECCCCEEEEEEEEEEEEEEEEEECCCCCCCHHEEEEHHHHHHHHHHCCHH
RFYEARRQQMMEPSIRWNGIHDWYAVMGEKTGADRYAFRLYVQSGVRWIWGGGLLMIAGA
HHHHHHHHHHCCCCCCCCCCHHHHHHHCCCCCCCHHHEEEEHHHCCEEEECCCHHHHHHH
LLSGWRGRKRDE
HHHCCCCCCCCC
>Mature Secondary Structure
MLTPLTAFAGVRLRWPAMMRLTCIGILAQFALLLLAFGVLTYCFLISDFSVIYVAQHSYS
CCCCCHHHCCCEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCE
LLSWELKLAAVWGGHEGSLLLWVLLLSAWSALFAWHYRQQTDPLFPLTLAVLSLMLAALL
EEEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHH
LFVVLWSDPFVRIFPPAIEGRDLNPMLQHPGLIFHPPLLYLGYGGLMVAASVALASLLRG
HHHHHHCCCCEEEECCCCCCCCCCHHHHCCCEEECCCHHHHCCHHHHHHHHHHHHHHHHC
EFDGACARICWRWALPGWSALTAGIILGSWWAYCELGWGGWWFWDPVENASLLPWLSATA
CCCHHHHHHHHHHCCCCHHHHHHHHHHHCHHHHEEECCCCEEEECCCCCCCCCHHHHHHH
LLHSLSLTRQREIFRHWSLLLAIVTLMLSLLGTLIVRSGILVSVHAFALDNVRAVPLFSL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHEEEHHHHHCCCCHHHHHHH
FALISLASLALYGWRARDGGAVVRFSGLSREMLILATLLLFCAVLLIVLVGTLYPMIYGL
HHHHHHHHHHHHCCEECCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LGWGRLSVGAPYFNRATLPFGLLMLVVIVLATFVSGKRVQLPALVAHAGVLLFAAGIVVS
HCCCHHCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHH
SVSRQEISLNLQPGQQVTLAGYTFRFERLDLQAKGNYTSEKAIVALFDHQQRIGELMPER
CCCHHHEEEEECCCCEEEEEEEEEEEEEEEEEECCCCCCCHHEEEEHHHHHHHHHHCCHH
RFYEARRQQMMEPSIRWNGIHDWYAVMGEKTGADRYAFRLYVQSGVRWIWGGGLLMIAGA
HHHHHHHHHHCCCCCCCCCCHHHHHHHCCCCCCCHHHEEEEHHHCCEEEECCCHHHHHHH
LLSGWRGRKRDE
HHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8057835; 8265357; 9278503 [H]