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The map label for this gene is pabA

Identifier: 157162837

GI number: 157162837

Start: 3534609

End: 3535172

Strand: Reverse

Name: pabA

Synonym: EcHS_A3557

Alternate gene names: 157162837

Gene position: 3535172-3534609 (Counterclockwise)

Preceding gene: 157162838

Following gene: 157162836

Centisome position: 76.13

GC content: 53.55

Gene sequence:

>564_bases
ATGATCCTGCTTATAGATAACTACGATTCTTTTACCTGGAACCTCTACCAGTACTTTTGTGAACTGGGGGCGGATGTGCT
GGTTAAGCGCAACGATGCGTTGACGCTGGCGGATATCGACGCCCTTAAACCACAAAAAATTGTCATCTCACCTGGCCCCT
GTACGCCAGATGAAGCCGGGATCTCCCTTGACGTTATTCGCCACTATGCCGGGCGCTTGCCGATTCTTGGCGTCTGCCTC
GGTCATCAGGCAATGGCGCAGGCATTTGGCGGTAAAGTTGTGCGCGCCGCAAAGGTCATGCACGGCAAAACCTCGCCGAT
TACACATAACGGTGAGGGCGTATTTCGGGGGCTGGCAAATCCACTTACCGTGACACGCTACCATTCGCTGGTGGTGGAAC
CTGACTCATTACCAGCGTGCTTTGACGTGACGGCCTGGAGCGAAACCCGCGAGATTATGGGGATTCGCCATCGCCAGTGG
GATCTGGAAGGTGTGCAGTTCCATCCAGAAAGTATTCTTAGCGAACAAGGACATCAACTGCTGGCTAATTTCCTGCATCG
CTGA

Upstream 100 bases:

>100_bases
ATGGGGGATCTCACCGCGCTGCAGATGATATTTAGCAAAGTGGTAAGCGAAGCCGGGGAATCTGAGTAAAATAGCGTGGT
TCTTTCGTACCGGAGCCGCC

Downstream 100 bases:

>100_bases
TTTCTGATTGCCATTCAGTGATTTTTTATGCATATTTTGTGATTATAATTTCAAATTTATTTATGCGTAACAGGGTGATC
ATGAGATGGCAATTGAACAA

Product: para-aminobenzoate synthase component II

Products: NA

Alternate protein names: ADC synthase

Number of amino acids: Translated: 187; Mature: 187

Protein sequence:

>187_residues
MILLIDNYDSFTWNLYQYFCELGADVLVKRNDALTLADIDALKPQKIVISPGPCTPDEAGISLDVIRHYAGRLPILGVCL
GHQAMAQAFGGKVVRAAKVMHGKTSPITHNGEGVFRGLANPLTVTRYHSLVVEPDSLPACFDVTAWSETREIMGIRHRQW
DLEGVQFHPESILSEQGHQLLANFLHR

Sequences:

>Translated_187_residues
MILLIDNYDSFTWNLYQYFCELGADVLVKRNDALTLADIDALKPQKIVISPGPCTPDEAGISLDVIRHYAGRLPILGVCL
GHQAMAQAFGGKVVRAAKVMHGKTSPITHNGEGVFRGLANPLTVTRYHSLVVEPDSLPACFDVTAWSETREIMGIRHRQW
DLEGVQFHPESILSEQGHQLLANFLHR
>Mature_187_residues
MILLIDNYDSFTWNLYQYFCELGADVLVKRNDALTLADIDALKPQKIVISPGPCTPDEAGISLDVIRHYAGRLPILGVCL
GHQAMAQAFGGKVVRAAKVMHGKTSPITHNGEGVFRGLANPLTVTRYHSLVVEPDSLPACFDVTAWSETREIMGIRHRQW
DLEGVQFHPESILSEQGHQLLANFLHR

Specific function: Catalyzes the biosynthesis of 4-amino-4-deoxychorismate (ADC) from chorismate and glutamine

COG id: COG0512

COG function: function code EH; Anthranilate/para-aminobenzoate synthases component II

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain

Homologues:

Organism=Escherichia coli, GI1789760, Length=187, Percent_Identity=100, Blast_Score=391, Evalue=1e-110,
Organism=Escherichia coli, GI1787517, Length=187, Percent_Identity=43.8502673796791, Blast_Score=150, Evalue=5e-38,
Organism=Saccharomyces cerevisiae, GI6322638, Length=186, Percent_Identity=52.1505376344086, Blast_Score=222, Evalue=3e-59,
Organism=Saccharomyces cerevisiae, GI6324361, Length=211, Percent_Identity=32.2274881516588, Blast_Score=77, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PABA_ECOLI (P00903)

Other databases:

- EMBL:   M28363
- EMBL:   K00030
- EMBL:   M32354
- EMBL:   U18997
- EMBL:   U00096
- EMBL:   AP009048
- PIR:   A01124
- RefSeq:   AP_004429.1
- RefSeq:   NP_417819.1
- ProteinModelPortal:   P00903
- SMR:   P00903
- DIP:   DIP-10433N
- STRING:   P00903
- EnsemblBacteria:   EBESCT00000003028
- EnsemblBacteria:   EBESCT00000003029
- EnsemblBacteria:   EBESCT00000015266
- GeneID:   947873
- GenomeReviews:   AP009048_GR
- GenomeReviews:   U00096_GR
- KEGG:   ecj:JW3323
- KEGG:   eco:b3360
- EchoBASE:   EB0676
- EcoGene:   EG10682
- eggNOG:   COG0512
- GeneTree:   EBGT00050000009095
- HOGENOM:   HBG292341
- OMA:   YNVVQYL
- ProtClustDB:   PRK08007
- BioCyc:   EcoCyc:PABASYN-COMPII-MONOMER
- BioCyc:   MetaCyc:PABASYN-COMPII-MONOMER
- Genevestigator:   P00903
- InterPro:   IPR006220
- InterPro:   IPR001317
- InterPro:   IPR011702
- InterPro:   IPR017926
- InterPro:   IPR000991
- InterPro:   IPR006221
- PRINTS:   PR00097
- PRINTS:   PR00099
- PRINTS:   PR00096
- TIGRFAMs:   TIGR00566

Pfam domain/function: PF00117 GATase

EC number: =2.6.1.85

Molecular weight: Translated: 20772; Mature: 20772

Theoretical pI: Translated: 6.60; Mature: 6.60

Prosite motif: PS51273 GATASE_TYPE_1; PS00442 GATASE_TYPE_I ; PS00678 WD_REPEATS_1

Important sites: ACT_SITE 79-79 ACT_SITE 168-168 ACT_SITE 170-170

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MILLIDNYDSFTWNLYQYFCELGADVLVKRNDALTLADIDALKPQKIVISPGPCTPDEAG
CEEEEECCCCCHHHHHHHHHHCCCCEEEECCCCEEEEECCCCCCCEEEECCCCCCCCCCC
ISLDVIRHYAGRLPILGVCLGHQAMAQAFGGKVVRAAKVMHGKTSPITHNGEGVFRGLAN
CCHHHHHHHHCCCCEEEHHHCHHHHHHHHCCHHHHHHHHHCCCCCCCEECCCHHHHHCCC
PLTVTRYHSLVVEPDSLPACFDVTAWSETREIMGIRHRQWDLEGVQFHPESILSEQGHQL
CEEEEEEEEEEECCCCCCCEEEECCHHHHHHHHCCCCCCCCCCCEEECHHHHHHHHHHHH
LANFLHR
HHHHHCC
>Mature Secondary Structure
MILLIDNYDSFTWNLYQYFCELGADVLVKRNDALTLADIDALKPQKIVISPGPCTPDEAG
CEEEEECCCCCHHHHHHHHHHCCCCEEEECCCCEEEEECCCCCCCEEEECCCCCCCCCCC
ISLDVIRHYAGRLPILGVCLGHQAMAQAFGGKVVRAAKVMHGKTSPITHNGEGVFRGLAN
CCHHHHHHHHCCCCEEEHHHCHHHHHHHHCCHHHHHHHHHCCCCCCCEECCCHHHHHCCC
PLTVTRYHSLVVEPDSLPACFDVTAWSETREIMGIRHRQWDLEGVQFHPESILSEQGHQL
CEEEEEEEEEEECCCCCCCEEEECCHHHHHHHHCCCCCCCCCCCEEECHHHHHHHHHHHH
LANFLHR
HHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 6350604; 2403545; 9278503; 2546924; 8096767