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The map label for this gene is ycgR [H]

Identifier: 157160697

GI number: 157160697

Start: 1292232

End: 1292966

Strand: Reverse

Name: ycgR [H]

Synonym: EcHS_A1297

Alternate gene names: 157160697

Gene position: 1292966-1292232 (Counterclockwise)

Preceding gene: 157160700

Following gene: 157160695

Centisome position: 27.84

GC content: 50.75

Gene sequence:

>735_bases
GTGAGTCATTACCATGAGCAGTTCCTGAAACAAAATCCGTTAGCCGTCCTGGGCGTGTTACGCGATTTGCACAAAGCCGC
AATTCCTTTGCGTCTCAGTTGGAATGGCGGGCAGCTGATCAGCAAAATACTGGCAATAACCCCGGATAAACTGGTGCTGG
ATTTCGGCAGTCAAGCCGAAGACAACATCGCCGTGCTAAAGGCACAGCACATTACCATTACCGCCGAAACTCAGGGTGCG
AAAGTCGAGTTTACTGTTGAACAACTACAGCAGAGTGAATACTTGCAGCTTCCGGCATTTATCACCGTACCGCCTCCCAC
CTTATGGTTTGTACAACGACGCCGATATTTCCGCATCTCCGCCCCACTCCATCCGCCTTATTTTTGCCAGACCAAACTGG
CGGATAACAGTACGTTACGTTTCCGCCTGTATGATTTGTCGTTAGGCGGCATGGGCGCATTACTGGAAACAGCAAAGCCT
GCCGAATTACAAGAAGGCATGCGCTTCGCTCAGATTGAAGTCAACATGAGGCAATGGGGTGTTTTTCACTTTGACGCCCA
GTTAATCTCCATCAGCGAGCGCAAAGTGATTGATGGCAAGAATGAAACCATCACCACTCCCCGTCTGAGCTTCCGTTTTC
TTAACGTCAGCCCGACGGTGGAGCGGCAATTACAGCGGATTATTTTCTCTCTCGAGCGAGAAGCCCGGGAAAAAGCGGAC
AAAGTGCGCGACTGA

Upstream 100 bases:

>100_bases
TTTAAGAACAACATGTAAAAAATATTCACGCATTTGTATTAAGTTTTGTTAACTGTGACCGATAAACCAAAGACAGTTTA
TCAGTCAGGAGTTTTTCCGC

Downstream 100 bases:

>100_bases
TTTACATCGCGTCCAGTGCCTGCTCAAGTTTGTAGATATAGCGCGGAGCCTGCGGCGCAGGGTGATTTCGCGCTACGTGT
TCGAGGAACTCGTCAGCATC

Product: putative type IV pilus assembly protein PilZ

Products: NA

Alternate protein names: Cyclic di-GMP binding protein YcgR [H]

Number of amino acids: Translated: 244; Mature: 243

Protein sequence:

>244_residues
MSHYHEQFLKQNPLAVLGVLRDLHKAAIPLRLSWNGGQLISKILAITPDKLVLDFGSQAEDNIAVLKAQHITITAETQGA
KVEFTVEQLQQSEYLQLPAFITVPPPTLWFVQRRRYFRISAPLHPPYFCQTKLADNSTLRFRLYDLSLGGMGALLETAKP
AELQEGMRFAQIEVNMRQWGVFHFDAQLISISERKVIDGKNETITTPRLSFRFLNVSPTVERQLQRIIFSLEREAREKAD
KVRD

Sequences:

>Translated_244_residues
MSHYHEQFLKQNPLAVLGVLRDLHKAAIPLRLSWNGGQLISKILAITPDKLVLDFGSQAEDNIAVLKAQHITITAETQGA
KVEFTVEQLQQSEYLQLPAFITVPPPTLWFVQRRRYFRISAPLHPPYFCQTKLADNSTLRFRLYDLSLGGMGALLETAKP
AELQEGMRFAQIEVNMRQWGVFHFDAQLISISERKVIDGKNETITTPRLSFRFLNVSPTVERQLQRIIFSLEREAREKAD
KVRD
>Mature_243_residues
SHYHEQFLKQNPLAVLGVLRDLHKAAIPLRLSWNGGQLISKILAITPDKLVLDFGSQAEDNIAVLKAQHITITAETQGAK
VEFTVEQLQQSEYLQLPAFITVPPPTLWFVQRRRYFRISAPLHPPYFCQTKLADNSTLRFRLYDLSLGGMGALLETAKPA
ELQEGMRFAQIEVNMRQWGVFHFDAQLISISERKVIDGKNETITTPRLSFRFLNVSPTVERQLQRIIFSLEREAREKADK
VRD

Specific function: Acts as a flagellar brake, regulating swimming and swarming in a bis-(3'-5') cyclic diguanylic acid (c-di-GMP)- dependent manner. When bound to c-di-GMP it binds to elements of the flagellar motor (MotA (PubMed:20303158) and/or FliG and FliM (PubMed:20346

COG id: COG5581

COG function: function code M; Predicted glycosyltransferase

Gene ontology:

Cell location: Bacterial flagellum basal body [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PilZ domain [H]

Homologues:

Organism=Escherichia coli, GI1787443, Length=244, Percent_Identity=99.1803278688525, Blast_Score=499, Evalue=1e-143,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009875
- InterPro:   IPR009926 [H]

Pfam domain/function: PF07238 PilZ; PF07317 YcgR [H]

EC number: NA

Molecular weight: Translated: 27957; Mature: 27825

Theoretical pI: Translated: 9.66; Mature: 9.66

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSHYHEQFLKQNPLAVLGVLRDLHKAAIPLRLSWNGGQLISKILAITPDKLVLDFGSQAE
CCHHHHHHHHCCCHHHHHHHHHHHHHHCEEEEECCCHHHHHHHHHCCCCCEEEECCCCCC
DNIAVLKAQHITITAETQGAKVEFTVEQLQQSEYLQLPAFITVPPPTLWFVQRRRYFRIS
CCEEEEEEEEEEEEEECCCCEEEEEHHHHCCCCCEECCEEEECCCCHHHHEECCCEEEEE
APLHPPYFCQTKLADNSTLRFRLYDLSLGGMGALLETAKPAELQEGMRFAQIEVNMRQWG
CCCCCCCEEEEECCCCCEEEEEEEEECCCCCHHHHHCCCCHHHHHCCEEEEEEEEHHHCE
VFHFDAQLISISERKVIDGKNETITTPRLSFRFLNVSPTVERQLQRIIFSLEREAREKAD
EEEECEEEEEECCCEEECCCCCEEECCCEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHH
KVRD
HCCC
>Mature Secondary Structure 
SHYHEQFLKQNPLAVLGVLRDLHKAAIPLRLSWNGGQLISKILAITPDKLVLDFGSQAE
CHHHHHHHHCCCHHHHHHHHHHHHHHCEEEEECCCHHHHHHHHHCCCCCEEEECCCCCC
DNIAVLKAQHITITAETQGAKVEFTVEQLQQSEYLQLPAFITVPPPTLWFVQRRRYFRIS
CCEEEEEEEEEEEEEECCCCEEEEEHHHHCCCCCEECCEEEECCCCHHHHEECCCEEEEE
APLHPPYFCQTKLADNSTLRFRLYDLSLGGMGALLETAKPAELQEGMRFAQIEVNMRQWG
CCCCCCCEEEEECCCCCEEEEEEEEECCCCCHHHHHCCCCHHHHHCCEEEEEEEEHHHCE
VFHFDAQLISISERKVIDGKNETITTPRLSFRFLNVSPTVERQLQRIIFSLEREAREKAD
EEEECEEEEEECCCEEECCCCCEEECCCEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHH
KVRD
HCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503 [H]