Click here to switch to the map view.

The map label for this gene is ldcA [H]

Identifier: 157160695

GI number: 157160695

Start: 1290605

End: 1291519

Strand: Reverse

Name: ldcA [H]

Synonym: EcHS_A1296

Alternate gene names: 157160695

Gene position: 1291519-1290605 (Counterclockwise)

Preceding gene: 157160697

Following gene: 157160694

Centisome position: 27.81

GC content: 53.01

Gene sequence:

>915_bases
ATGTCTCTGTTTCACTTAATTGCCCCATCGGGTTACTGCATTAAACAGCACGCCGCGCTGCGTGGTATCCAGCGCCTTAC
CGACGCGGGGCATCAAGTCAATAACGTCGAGGTTATTGCCCGTCGTTGTGAGCGTTTTGCGGGTACGGAAACAGAGCGTC
TGGAAGATCTCAATTCCCTTGCCAGACTGACTACCCCCAACACCATCGTGCTGGCTGTACGCGGCGGTTACGGTGCCAGT
CGTTTACTGGCAGATATTGACTGGCAGGCACTGGTGGCTCGCCAACAACATGACCCGTTGCTCATTTGCGGACATAGCGA
TTTTACCGCCATTCAGTGCGGTCTTCTGGCGCATGGCAATGTCATCACCTTTAGCGGCCCCATGCTGGTAGCAAACTTTG
GCGCGGATGAACTGAACGCCTTTACCGAGCACCATTTCTGGCTGGCGTTACGCAATGAAACCTTCACCATTGAATGGCAA
GGGGAAGGCCCGACATGTCGGGCCGAAGGCACGTTGTGGGGAGGCAATCTTGCGATGCTGATTTCACTGATCGGTACACC
GTGGATGCCAAAAATTGAGAACGGTATTCTGGTGCTGGAAGATATTAACGAGCACCCTTTCCGGGTCGAACGTATGCTGT
TGCAGCTCTATCATGCGGGAATTTTGCCACGTCAGAAGGCGATTATTCTCGGTAGCTTTAGCGGCAGCACGCCCAATGAT
TATGACGCAGGTTACAACCTGGAGTCAGTGTACGCGTTTTTGCGTTCCCGCCTGTCGATTCCACTCATTACCGGCCTCGA
TTTTGGTCATGAACAACGCACGGTTACGCTGCCGTTGGGCGCACATGCCATTCTGAATAACACCCGGGAAGGCACTCAGT
TGACGATTAGCGGTCATCCTGTTCTTAAAATGTAA

Upstream 100 bases:

>100_bases
CTTCTTCTATCCTTATAGCCTGGAATCGCCGTTGAGGTGACAACGGCACACCTAAGCGGCATGATATACATTTCGTTTCT
GTTGTCAGCAAGGAATTGCC

Downstream 100 bases:

>100_bases
AAAAATCACGCTTCAGGGCTAAGTAAAACGCTGTCTCTGCCGCTAATATGGTAGGGTTTATATAAGAGACAGCGTAATCA
GGAGTAACCGACCGTTGGAT

Product: L,D-carboxypeptidase A

Products: NA

Alternate protein names: LD-carboxypeptidase A; Muramoyltetrapeptide carboxypeptidase [H]

Number of amino acids: Translated: 304; Mature: 303

Protein sequence:

>304_residues
MSLFHLIAPSGYCIKQHAALRGIQRLTDAGHQVNNVEVIARRCERFAGTETERLEDLNSLARLTTPNTIVLAVRGGYGAS
RLLADIDWQALVARQQHDPLLICGHSDFTAIQCGLLAHGNVITFSGPMLVANFGADELNAFTEHHFWLALRNETFTIEWQ
GEGPTCRAEGTLWGGNLAMLISLIGTPWMPKIENGILVLEDINEHPFRVERMLLQLYHAGILPRQKAIILGSFSGSTPND
YDAGYNLESVYAFLRSRLSIPLITGLDFGHEQRTVTLPLGAHAILNNTREGTQLTISGHPVLKM

Sequences:

>Translated_304_residues
MSLFHLIAPSGYCIKQHAALRGIQRLTDAGHQVNNVEVIARRCERFAGTETERLEDLNSLARLTTPNTIVLAVRGGYGAS
RLLADIDWQALVARQQHDPLLICGHSDFTAIQCGLLAHGNVITFSGPMLVANFGADELNAFTEHHFWLALRNETFTIEWQ
GEGPTCRAEGTLWGGNLAMLISLIGTPWMPKIENGILVLEDINEHPFRVERMLLQLYHAGILPRQKAIILGSFSGSTPND
YDAGYNLESVYAFLRSRLSIPLITGLDFGHEQRTVTLPLGAHAILNNTREGTQLTISGHPVLKM
>Mature_303_residues
SLFHLIAPSGYCIKQHAALRGIQRLTDAGHQVNNVEVIARRCERFAGTETERLEDLNSLARLTTPNTIVLAVRGGYGASR
LLADIDWQALVARQQHDPLLICGHSDFTAIQCGLLAHGNVITFSGPMLVANFGADELNAFTEHHFWLALRNETFTIEWQG
EGPTCRAEGTLWGGNLAMLISLIGTPWMPKIENGILVLEDINEHPFRVERMLLQLYHAGILPRQKAIILGSFSGSTPNDY
DAGYNLESVYAFLRSRLSIPLITGLDFGHEQRTVTLPLGAHAILNNTREGTQLTISGHPVLKM

Specific function: Releases the terminal D-alanine residue from the cytoplasmic tetrapeptide recycling product L-Ala-gamma-D-Glu-meso- Dap-D-Ala. Can also cleave D-Ala from murein derivatives containing the tetrapeptide, i.e. MurNAc-tetrapeptide, UDP-MurNAc- tetrapeptide, G

COG id: COG1619

COG function: function code V; Uncharacterized proteins, homologs of microcin C7 resistance protein MccF

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S66 family [H]

Homologues:

Organism=Escherichia coli, GI1787441, Length=304, Percent_Identity=100, Blast_Score=625, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003507 [H]

Pfam domain/function: PF02016 Peptidase_S66 [H]

EC number: NA

Molecular weight: Translated: 33568; Mature: 33437

Theoretical pI: Translated: 6.57; Mature: 6.57

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLFHLIAPSGYCIKQHAALRGIQRLTDAGHQVNNVEVIARRCERFAGTETERLEDLNSL
CCEEEEECCCCCEEHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHH
ARLTTPNTIVLAVRGGYGASRLLADIDWQALVARQQHDPLLICGHSDFTAIQCGLLAHGN
HHHCCCCEEEEEEECCCCHHHHHHCCCHHHHHHHCCCCCEEEECCCCCCEEEEEEEECCC
VITFSGPMLVANFGADELNAFTEHHFWLALRNETFTIEWQGEGPTCRAEGTLWGGNLAML
EEEEECCEEEEECCCHHHHHHHCCEEEEEEECCEEEEEECCCCCEEEECCEEECCCHHHH
ISLIGTPWMPKIENGILVLEDINEHPFRVERMLLQLYHAGILPRQKAIILGSFSGSTPND
HHHHCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCC
YDAGYNLESVYAFLRSRLSIPLITGLDFGHEQRTVTLPLGAHAILNNTREGTQLTISGHP
CCCCCCHHHHHHHHHHCCCCEEEECCCCCCCCEEEEEECCCHHHHCCCCCCCEEEECCCC
VLKM
EECC
>Mature Secondary Structure 
SLFHLIAPSGYCIKQHAALRGIQRLTDAGHQVNNVEVIARRCERFAGTETERLEDLNSL
CEEEEECCCCCEEHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHH
ARLTTPNTIVLAVRGGYGASRLLADIDWQALVARQQHDPLLICGHSDFTAIQCGLLAHGN
HHHCCCCEEEEEEECCCCHHHHHHCCCHHHHHHHCCCCCEEEECCCCCCEEEEEEEECCC
VITFSGPMLVANFGADELNAFTEHHFWLALRNETFTIEWQGEGPTCRAEGTLWGGNLAML
EEEEECCEEEEECCCHHHHHHHCCEEEEEEECCEEEEEECCCCCEEEECCEEECCCHHHH
ISLIGTPWMPKIENGILVLEDINEHPFRVERMLLQLYHAGILPRQKAIILGSFSGSTPND
HHHHCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCC
YDAGYNLESVYAFLRSRLSIPLITGLDFGHEQRTVTLPLGAHAILNNTREGTQLTISGHP
CCCCCCHHHHHHHHHHCCCCEEEECCCCCCCCEEEEEECCCHHHHCCCCCCCEEEECCCC
VLKM
EECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]