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The map label for this gene is treA [H]

Identifier: 157160700

GI number: 157160700

Start: 1294118

End: 1295815

Strand: Reverse

Name: treA [H]

Synonym: EcHS_A1301

Alternate gene names: 157160700

Gene position: 1295815-1294118 (Counterclockwise)

Preceding gene: 157160702

Following gene: 157160697

Centisome position: 27.91

GC content: 52.24

Gene sequence:

>1698_bases
ATGAAATCCCCCGCACCTTCTCGCCCGCAAAAAATGGCGTTAATTCCAGCCTGCATCTTTTTGTGTTTCGCTGCGCTATC
GGTGCAGGCAGAAGAAACACCGGTAACACCACAGCCGCCTGATATTTTATTAGGGCCGCTGTTTAATGATGTGCAAAACG
CCAAACTTTTTCCGGACCAAAAAACCTTTGCCGATGCCGTGCCGAACAGCGATCCGCTGATGATCCTTGCTGATTATCGG
ATGCAGCAAAACCAGAGCGGATTTGATCTGCGCCATTTCGTTAACGTCAATTTCACCCTGCCGAAAGAGGGCGAGAAATA
TGTTCCGCCAGAGGGACAGTCACTGCGCGAACATATTGACGGACTTTGGCCGGTATTAACGCGTTCTACCGAAAACACCG
AAAAATGGGATTCTCTGTTACCGCTGCCGGAACCTTATGTCGTGCCGGGCGGACGTTTTCGCGAGGTATATTACTGGGAC
AGTTACTTCACCATGTTAGGACTTGCCGAAAGCGGTCACTGGGATAAAGTCGCGGATATGGTGGCCAATTTTGCTCATGA
AATAGACACTTACGGTCATATTCCCAACGGCAACCGCAGTTACTATTTAAGCCGCTCGCAACCGCCCTTCTTTGCCCTGA
TGGTAGAGTTACTGGCGCAGCATGAAGGCGATGCCGCGTTGAAGCAATACCTGCCGCAAATGCAAAAAGAATATGCTTAC
TGGATGGACGGTGTTGAAAACCTGCAAGCCGGACAACAGGAAAAACGCGTTGTAAAACTTCAGGATGGTACCCTTCTCAA
CCGCTACTGGGACGATCGCGATACGCCACGACCAGAGTCATGGGTGGAAGATATTGCCACCGCCAAAAGCAATCCGAATC
GACCTGCCACTGAAATTTACCGCGACCTGCGCTCTGCCGCTGCGTCTGGCTGGGATTTCAGCTCGCGCTGGATGGACAAC
CCGCAGCAGTTAAATACCTTACGCACCACCAGCATCGTACCGGTCGATCTGAACAGCCTGATGTTTAAAATGGAAAAAAT
CCTCGCCCGCGCCAGCAAAGCTGCCGGAGATAACGCGATGGCAAACCAGTACGAAACGCTGGCAAATGCCCGTCAAAAAG
GGATCGAAAAATACCTGTGGAACGATCAACAAGGCTGGTATGCCGATTACGACCTGAAAAGTCATAAAGTGCGCAATCAG
TTAACCGCGGCCGCCCTGTTCCCGCTGTACGTCAATGCGGCAGCGAAAGATCGCGCCAACAAAATGGCGACGGCGACGAA
AACACATCTGCTGCAACCCGGCGGCCTGAACACCACGTCGGTGAAAAGTGGGCAACAATGGGATGCGCCAAATGGCTGGG
CACCGTTACAGTGGGTCGCGACAGAAGGATTACAAAACTACGGGCAAAAAGAGGTGGCGATGGACATTAGCTGGCACTTC
CTGACCAATGTTCAGCACACCTATGACCGGGAGAAAAAGCTGGTGGAAAAATATGATGTCAGCACCACCGGAACGGGGGG
CGGCGGTGGCGAATATCCATTACAGGATGGCTTTGGCTGGACCAATGGCGTGACGCTGAAAATGCTGGATTTGATCTGCC
CGAAAGAGCAACCGTGTGACAATGTTCCGGCGACGCGTCCGACCGTTAAGTCAGCAACGACGCAACCCTCAACCAAAGAG
GCACAACCCACACCTTAA

Upstream 100 bases:

>100_bases
GCAGAATGAGATTTCGATCATGCAGCTAGTGCGATCCTGAACTAAGGTTTTCTGATACTTGAATACCGTTTTATTCGGTT
TCGCCAAAGGAGAATGATTG

Downstream 100 bases:

>100_bases
CCAGCGCTTACTCCGACAACGTCATTCGGCTTCTCCGGATGGCGTTGTCGGCAGATAACACCATAGCTATGGTTTTTTCT
ACACAGACGGTTTTTCAAGT

Product: trehalase

Products: NA

Alternate protein names: Alpha,alpha-trehalase; Alpha,alpha-trehalose glucohydrolase [H]

Number of amino acids: Translated: 565; Mature: 565

Protein sequence:

>565_residues
MKSPAPSRPQKMALIPACIFLCFAALSVQAEETPVTPQPPDILLGPLFNDVQNAKLFPDQKTFADAVPNSDPLMILADYR
MQQNQSGFDLRHFVNVNFTLPKEGEKYVPPEGQSLREHIDGLWPVLTRSTENTEKWDSLLPLPEPYVVPGGRFREVYYWD
SYFTMLGLAESGHWDKVADMVANFAHEIDTYGHIPNGNRSYYLSRSQPPFFALMVELLAQHEGDAALKQYLPQMQKEYAY
WMDGVENLQAGQQEKRVVKLQDGTLLNRYWDDRDTPRPESWVEDIATAKSNPNRPATEIYRDLRSAAASGWDFSSRWMDN
PQQLNTLRTTSIVPVDLNSLMFKMEKILARASKAAGDNAMANQYETLANARQKGIEKYLWNDQQGWYADYDLKSHKVRNQ
LTAAALFPLYVNAAAKDRANKMATATKTHLLQPGGLNTTSVKSGQQWDAPNGWAPLQWVATEGLQNYGQKEVAMDISWHF
LTNVQHTYDREKKLVEKYDVSTTGTGGGGGEYPLQDGFGWTNGVTLKMLDLICPKEQPCDNVPATRPTVKSATTQPSTKE
AQPTP

Sequences:

>Translated_565_residues
MKSPAPSRPQKMALIPACIFLCFAALSVQAEETPVTPQPPDILLGPLFNDVQNAKLFPDQKTFADAVPNSDPLMILADYR
MQQNQSGFDLRHFVNVNFTLPKEGEKYVPPEGQSLREHIDGLWPVLTRSTENTEKWDSLLPLPEPYVVPGGRFREVYYWD
SYFTMLGLAESGHWDKVADMVANFAHEIDTYGHIPNGNRSYYLSRSQPPFFALMVELLAQHEGDAALKQYLPQMQKEYAY
WMDGVENLQAGQQEKRVVKLQDGTLLNRYWDDRDTPRPESWVEDIATAKSNPNRPATEIYRDLRSAAASGWDFSSRWMDN
PQQLNTLRTTSIVPVDLNSLMFKMEKILARASKAAGDNAMANQYETLANARQKGIEKYLWNDQQGWYADYDLKSHKVRNQ
LTAAALFPLYVNAAAKDRANKMATATKTHLLQPGGLNTTSVKSGQQWDAPNGWAPLQWVATEGLQNYGQKEVAMDISWHF
LTNVQHTYDREKKLVEKYDVSTTGTGGGGGEYPLQDGFGWTNGVTLKMLDLICPKEQPCDNVPATRPTVKSATTQPSTKE
AQPTP
>Mature_565_residues
MKSPAPSRPQKMALIPACIFLCFAALSVQAEETPVTPQPPDILLGPLFNDVQNAKLFPDQKTFADAVPNSDPLMILADYR
MQQNQSGFDLRHFVNVNFTLPKEGEKYVPPEGQSLREHIDGLWPVLTRSTENTEKWDSLLPLPEPYVVPGGRFREVYYWD
SYFTMLGLAESGHWDKVADMVANFAHEIDTYGHIPNGNRSYYLSRSQPPFFALMVELLAQHEGDAALKQYLPQMQKEYAY
WMDGVENLQAGQQEKRVVKLQDGTLLNRYWDDRDTPRPESWVEDIATAKSNPNRPATEIYRDLRSAAASGWDFSSRWMDN
PQQLNTLRTTSIVPVDLNSLMFKMEKILARASKAAGDNAMANQYETLANARQKGIEKYLWNDQQGWYADYDLKSHKVRNQ
LTAAALFPLYVNAAAKDRANKMATATKTHLLQPGGLNTTSVKSGQQWDAPNGWAPLQWVATEGLQNYGQKEVAMDISWHF
LTNVQHTYDREKKLVEKYDVSTTGTGGGGGEYPLQDGFGWTNGVTLKMLDLICPKEQPCDNVPATRPTVKSATTQPSTKE
AQPTP

Specific function: Provides the cells with the ability to utilize trehalose at high osmolarity by splitting it into glucose molecules that can subsequently be taken up by the phosphotransferase-mediated uptake system [H]

COG id: COG1626

COG function: function code G; Neutral trehalase

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 37 family [H]

Homologues:

Organism=Homo sapiens, GI116284412, Length=526, Percent_Identity=33.8403041825095, Blast_Score=265, Evalue=1e-70,
Organism=Escherichia coli, GI1787447, Length=565, Percent_Identity=100, Blast_Score=1178, Evalue=0.0,
Organism=Escherichia coli, GI1789936, Length=487, Percent_Identity=51.129363449692, Blast_Score=513, Evalue=1e-146,
Organism=Caenorhabditis elegans, GI17542196, Length=520, Percent_Identity=34.0384615384615, Blast_Score=270, Evalue=2e-72,
Organism=Caenorhabditis elegans, GI25148109, Length=574, Percent_Identity=31.7073170731707, Blast_Score=248, Evalue=5e-66,
Organism=Caenorhabditis elegans, GI25141398, Length=527, Percent_Identity=31.6888045540797, Blast_Score=242, Evalue=4e-64,
Organism=Caenorhabditis elegans, GI17565078, Length=545, Percent_Identity=31.5596330275229, Blast_Score=234, Evalue=7e-62,
Organism=Caenorhabditis elegans, GI71987755, Length=403, Percent_Identity=29.0322580645161, Blast_Score=183, Evalue=2e-46,
Organism=Saccharomyces cerevisiae, GI6320204, Length=436, Percent_Identity=30.045871559633, Blast_Score=159, Evalue=8e-40,
Organism=Saccharomyces cerevisiae, GI6319473, Length=428, Percent_Identity=30.3738317757009, Blast_Score=153, Evalue=7e-38,
Organism=Drosophila melanogaster, GI24656680, Length=528, Percent_Identity=34.280303030303, Blast_Score=273, Evalue=2e-73,
Organism=Drosophila melanogaster, GI24656675, Length=528, Percent_Identity=34.280303030303, Blast_Score=273, Evalue=2e-73,
Organism=Drosophila melanogaster, GI24656661, Length=528, Percent_Identity=34.280303030303, Blast_Score=272, Evalue=5e-73,
Organism=Drosophila melanogaster, GI17933716, Length=528, Percent_Identity=34.280303030303, Blast_Score=272, Evalue=5e-73,
Organism=Drosophila melanogaster, GI24656670, Length=528, Percent_Identity=34.280303030303, Blast_Score=272, Evalue=5e-73,
Organism=Drosophila melanogaster, GI24656685, Length=502, Percent_Identity=33.6653386454183, Blast_Score=255, Evalue=6e-68,
Organism=Drosophila melanogaster, GI22024178, Length=527, Percent_Identity=31.1195445920304, Blast_Score=237, Evalue=2e-62,
Organism=Drosophila melanogaster, GI45551104, Length=367, Percent_Identity=32.425068119891, Blast_Score=172, Evalue=4e-43,
Organism=Drosophila melanogaster, GI28573474, Length=314, Percent_Identity=28.343949044586, Blast_Score=121, Evalue=1e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008928
- InterPro:   IPR001661
- InterPro:   IPR018232 [H]

Pfam domain/function: PF01204 Trehalase [H]

EC number: =3.2.1.28 [H]

Molecular weight: Translated: 63637; Mature: 63637

Theoretical pI: Translated: 5.69; Mature: 5.69

Prosite motif: PS00927 TREHALASE_1 ; PS00928 TREHALASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSPAPSRPQKMALIPACIFLCFAALSVQAEETPVTPQPPDILLGPLFNDVQNAKLFPDQ
CCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEECHHHHHHHCCCCCCCH
KTFADAVPNSDPLMILADYRMQQNQSGFDLRHFVNVNFTLPKEGEKYVPPEGQSLREHID
HHHHHHCCCCCCEEEEECCHHHCCCCCCCEEEEEEEEEECCCCCCCCCCCCCHHHHHHHH
GLWPVLTRSTENTEKWDSLLPLPEPYVVPGGRFREVYYWDSYFTMLGLAESGHWDKVADM
HHHHHHHCCCCCHHHHHHCCCCCCCEECCCCCEEEEEEECHHHHHHHCCCCCCHHHHHHH
VANFAHEIDTYGHIPNGNRSYYLSRSQPPFFALMVELLAQHEGDAALKQYLPQMQKEYAY
HHHHHHHHHCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
WMDGVENLQAGQQEKRVVKLQDGTLLNRYWDDRDTPRPESWVEDIATAKSNPNRPATEIY
HHHHHHHHHCCCHHCCEEEECCCEEHHHHCCCCCCCCHHHHHHHHHHHCCCCCCCHHHHH
RDLRSAAASGWDFSSRWMDNPQQLNTLRTTSIVPVDLNSLMFKMEKILARASKAAGDNAM
HHHHHHHCCCCCCHHHHCCCHHHHHHHHHCEEEEECHHHHHHHHHHHHHHHHHHCCCCHH
ANQYETLANARQKGIEKYLWNDQQGWYADYDLKSHKVRNQLTAAALFPLYVNAAAKDRAN
HHHHHHHHHHHHHHHHHHHCCCCCCCEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KMATATKTHLLQPGGLNTTSVKSGQQWDAPNGWAPLQWVATEGLQNYGQKEVAMDISWHF
HHHHHHHHHEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEEEHHH
LTNVQHTYDREKKLVEKYDVSTTGTGGGGGEYPLQDGFGWTNGVTLKMLDLICPKEQPCD
HHCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCC
NVPATRPTVKSATTQPSTKEAQPTP
CCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MKSPAPSRPQKMALIPACIFLCFAALSVQAEETPVTPQPPDILLGPLFNDVQNAKLFPDQ
CCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEECHHHHHHHCCCCCCCH
KTFADAVPNSDPLMILADYRMQQNQSGFDLRHFVNVNFTLPKEGEKYVPPEGQSLREHID
HHHHHHCCCCCCEEEEECCHHHCCCCCCCEEEEEEEEEECCCCCCCCCCCCCHHHHHHHH
GLWPVLTRSTENTEKWDSLLPLPEPYVVPGGRFREVYYWDSYFTMLGLAESGHWDKVADM
HHHHHHHCCCCCHHHHHHCCCCCCCEECCCCCEEEEEEECHHHHHHHCCCCCCHHHHHHH
VANFAHEIDTYGHIPNGNRSYYLSRSQPPFFALMVELLAQHEGDAALKQYLPQMQKEYAY
HHHHHHHHHCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
WMDGVENLQAGQQEKRVVKLQDGTLLNRYWDDRDTPRPESWVEDIATAKSNPNRPATEIY
HHHHHHHHHCCCHHCCEEEECCCEEHHHHCCCCCCCCHHHHHHHHHHHCCCCCCCHHHHH
RDLRSAAASGWDFSSRWMDNPQQLNTLRTTSIVPVDLNSLMFKMEKILARASKAAGDNAM
HHHHHHHCCCCCCHHHHCCCHHHHHHHHHCEEEEECHHHHHHHHHHHHHHHHHHCCCCHH
ANQYETLANARQKGIEKYLWNDQQGWYADYDLKSHKVRNQLTAAALFPLYVNAAAKDRAN
HHHHHHHHHHHHHHHHHHHCCCCCCCEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KMATATKTHLLQPGGLNTTSVKSGQQWDAPNGWAPLQWVATEGLQNYGQKEVAMDISWHF
HHHHHHHHHEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEEEHHH
LTNVQHTYDREKKLVEKYDVSTTGTGGGGGEYPLQDGFGWTNGVTLKMLDLICPKEQPCD
HHCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCC
NVPATRPTVKSATTQPSTKEAQPTP
CCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA