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The map label for this gene is hyaD

Identifier: 157160494

GI number: 157160494

Start: 1097384

End: 1097971

Strand: Direct

Name: hyaD

Synonym: EcHS_A1083

Alternate gene names: 157160494

Gene position: 1097384-1097971 (Clockwise)

Preceding gene: 157160493

Following gene: 157160495

Centisome position: 23.63

GC content: 58.84

Gene sequence:

>588_bases
ATGAGCGAGCAACGCGTGGTGGTCATGGGGCTGGGCAACCTGCTGTGGGCCGATGAAGGCTTCGGCGTGCGGGTGGCGGA
ACGGCTGTATGCCCATTACCACTGGCCCGAGTATGTGGAGATTGTCGATGGCGGTACTCAGGGACTGAACTTGCTGGGGT
ATGTCGAAAGCGCCAGCCATCTGTTGATTCTCGATGCCATTGACTACGGGCTGGAACCTGGAACGCTGCGAACCTATGCC
GGAGAACGCATTCCGGCTTATCTCAGCGCGAAGAAAATGAGCCTGCATCAGAACAGTTTCTCCGAAGTGTTGGCGCTGGC
GGATATCCGCGGACATCTGCCAGCACATATTGCCCTCGTCGGTCTGCAACCCGCAATGCTCGACGACTACGGCGGTAGCC
TGAGCGAACTGGCACGGGAGCAACTGCCCGCTGCGGAACAGGCGGCGCTGGCGCAGCTTGCTGCGTGGGGAATTGTGCCG
CAACCGGCTAATGAATCGCGCTGTCTCAATTATGACTGTCTGTCGATGGAAAATTACGAAGGCGTTCGCTTGCGCCAGTA
CCGGATGACACAGGAGGAGCAGGGATGA

Upstream 100 bases:

>100_bases
CGCTGCGTGAAGACATCATGTCCGACGACACGGTGATCTCCACCATGGTCAACGGCTACCGTAGCCACAAATTTGGCAAA
ATAAGTAACAAGGAGCGTTC

Downstream 100 bases:

>100_bases
GCAACGACACGCCATTTGATGCGTTGTGGCAACGAATGCTGGCGCGCGGCTGGACGCCAGTCAGTGAATCCCGTCTTGAC
GACTGGCTTACGCAAGCGCC

Product: hydrogenase 1 maturation protease

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 195; Mature: 194

Protein sequence:

>195_residues
MSEQRVVVMGLGNLLWADEGFGVRVAERLYAHYHWPEYVEIVDGGTQGLNLLGYVESASHLLILDAIDYGLEPGTLRTYA
GERIPAYLSAKKMSLHQNSFSEVLALADIRGHLPAHIALVGLQPAMLDDYGGSLSELAREQLPAAEQAALAQLAAWGIVP
QPANESRCLNYDCLSMENYEGVRLRQYRMTQEEQG

Sequences:

>Translated_195_residues
MSEQRVVVMGLGNLLWADEGFGVRVAERLYAHYHWPEYVEIVDGGTQGLNLLGYVESASHLLILDAIDYGLEPGTLRTYA
GERIPAYLSAKKMSLHQNSFSEVLALADIRGHLPAHIALVGLQPAMLDDYGGSLSELAREQLPAAEQAALAQLAAWGIVP
QPANESRCLNYDCLSMENYEGVRLRQYRMTQEEQG
>Mature_194_residues
SEQRVVVMGLGNLLWADEGFGVRVAERLYAHYHWPEYVEIVDGGTQGLNLLGYVESASHLLILDAIDYGLEPGTLRTYAG
ERIPAYLSAKKMSLHQNSFSEVLALADIRGHLPAHIALVGLQPAMLDDYGGSLSELAREQLPAAEQAALAQLAAWGIVPQ
PANESRCLNYDCLSMENYEGVRLRQYRMTQEEQG

Specific function: Protease involved in the C-terminal processing of hyaB, the large subunit of hydrogenase 1 (Potential)

COG id: COG0680

COG function: function code C; Ni,Fe-hydrogenase maturation factor

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase A31 family

Homologues:

Organism=Escherichia coli, GI1787209, Length=195, Percent_Identity=100, Blast_Score=396, Evalue=1e-112,
Organism=Escherichia coli, GI1789367, Length=155, Percent_Identity=39.3548387096774, Blast_Score=101, Evalue=3e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HYAD_ECOLI (P19930)

Other databases:

- EMBL:   M34825
- EMBL:   U00096
- EMBL:   AP009048
- PIR:   JV0075
- RefSeq:   AP_001604.1
- RefSeq:   NP_415494.1
- ProteinModelPortal:   P19930
- SMR:   P19930
- DIP:   DIP-9960N
- MINT:   MINT-1321337
- STRING:   P19930
- MEROPS:   A31.002
- EnsemblBacteria:   EBESCT00000004797
- EnsemblBacteria:   EBESCT00000017466
- GeneID:   945575
- GenomeReviews:   AP009048_GR
- GenomeReviews:   U00096_GR
- KEGG:   ecj:JW0957
- KEGG:   eco:b0975
- EchoBASE:   EB0466
- EcoGene:   EG10471
- eggNOG:   COG0680
- GeneTree:   EBGT00050000009915
- HOGENOM:   HBG730505
- OMA:   HEVEMLH
- ProtClustDB:   PRK10264
- BioCyc:   EcoCyc:EG10471-MONOMER
- Genevestigator:   P19930
- InterPro:   IPR004419
- InterPro:   IPR000671
- Gene3D:   G3DSA:3.40.50.1450
- PRINTS:   PR00446
- TIGRFAMs:   TIGR00140
- TIGRFAMs:   TIGR00072

Pfam domain/function: PF01750 HycI

EC number: 3.4.24.- [C]

Molecular weight: Translated: 21547; Mature: 21416

Theoretical pI: Translated: 4.55; Mature: 4.55

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEQRVVVMGLGNLLWADEGFGVRVAERLYAHYHWPEYVEIVDGGTQGLNLLGYVESASH
CCCCEEEEEECCCEEECCCCCCHHHHHHHHHHCCCCCEEEEECCCCCCCEEEEEECCCCE
LLILDAIDYGLEPGTLRTYAGERIPAYLSAKKMSLHQNSFSEVLALADIRGHLPAHIALV
EEEEEEHHCCCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEE
GLQPAMLDDYGGSLSELAREQLPAAEQAALAQLAAWGIVPQPANESRCLNYDCLSMENYE
ECCCHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCCCCEEECCCCCCC
GVRLRQYRMTQEEQG
CCEEEEEECCCCCCC
>Mature Secondary Structure 
SEQRVVVMGLGNLLWADEGFGVRVAERLYAHYHWPEYVEIVDGGTQGLNLLGYVESASH
CCCEEEEEECCCEEECCCCCCHHHHHHHHHHCCCCCEEEEECCCCCCCEEEEEECCCCE
LLILDAIDYGLEPGTLRTYAGERIPAYLSAKKMSLHQNSFSEVLALADIRGHLPAHIALV
EEEEEEHHCCCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEE
GLQPAMLDDYGGSLSELAREQLPAAEQAALAQLAAWGIVPQPANESRCLNYDCLSMENYE
ECCCHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCCCCEEECCCCCCC
GVRLRQYRMTQEEQG
CCEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Ni [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2180913; 8905232; 9278503