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The map label for this gene is hyaC

Identifier: 157160493

GI number: 157160493

Start: 1096680

End: 1097387

Strand: Direct

Name: hyaC

Synonym: EcHS_A1082

Alternate gene names: 157160493

Gene position: 1096680-1097387 (Clockwise)

Preceding gene: 157160492

Following gene: 157160494

Centisome position: 23.62

GC content: 51.84

Gene sequence:

>708_bases
ATGCAACAGAAAAGCGACAACGTTGTCAGCCACTATGTCTTTGAAGCGCCAGTGCGCATCTGGCACTGGTTGACGGTGTT
ATGCATGGCGGTGTTGATGGTCACCGGATACTTTATCGGCAAGCCGCTACCTTCCGTCAGCGGCGAGGCGACGTATCTGT
TCTATATGGGCTACATCAGGTTAATTCACTTCAGCGCCGGGATGGTTTTTACCGTGGTTTTGCTGATGCGGATCTACTGG
GCTTTTGTTGGCAATCGATACTCCCGCGAGCTGTTTATCGTGCCGGTATGGCGTAAAAGCTGGTGGCAGGGCGTGTGGTA
TGAAATCCGCTGGTATCTGTTTCTGGCAAAACGTCCGAGTGCCGATATAGGCCATAATCCCATCGCCCAGGCGGCGATGT
TCGGCTATTTCCTGATGTCGGTCTTTATGATCATCACTGGTTTTGCGCTGTACAGCGAACACAGCCAGTACGCTATTTTT
GCGCCGTTCCGTTATGTGGTGGAATTTTTCTACTGGACGGGTGGCAACTCAATGGACATTCACAGCTGGCATCGGCTGGG
GATGTGGCTGATTGGCGCGTTTGTGATCGGTCATGTCTACATGGCGCTGCGTGAAGACATCATGTCCGACGACACGGTGA
TCTCCACCATGGTCAACGGCTACCGTAGCCACAAATTTGGCAAAATAAGTAACAAGGAGCGTTCATGA

Upstream 100 bases:

>100_bases
CAGCTTTGACCCGTGCCTCGCCTGTTCAACACACGTGCTGGGCGACGACGGTAGCGAGCTGATCTCCGTGCAGGTGCGTT
AACAGCGAAGGAGAATCATC

Downstream 100 bases:

>100_bases
GCGAGCAACGCGTGGTGGTCATGGGGCTGGGCAACCTGCTGTGGGCCGATGAAGGCTTCGGCGTGCGGGTGGCGGAACGG
CTGTATGCCCATTACCACTG

Product: hydrogenase 1 b-type cytochrome subunit

Products: oxidized ferredoxin; H2 [C]

Alternate protein names: NA

Number of amino acids: Translated: 235; Mature: 235

Protein sequence:

>235_residues
MQQKSDNVVSHYVFEAPVRIWHWLTVLCMAVLMVTGYFIGKPLPSVSGEATYLFYMGYIRLIHFSAGMVFTVVLLMRIYW
AFVGNRYSRELFIVPVWRKSWWQGVWYEIRWYLFLAKRPSADIGHNPIAQAAMFGYFLMSVFMIITGFALYSEHSQYAIF
APFRYVVEFFYWTGGNSMDIHSWHRLGMWLIGAFVIGHVYMALREDIMSDDTVISTMVNGYRSHKFGKISNKERS

Sequences:

>Translated_235_residues
MQQKSDNVVSHYVFEAPVRIWHWLTVLCMAVLMVTGYFIGKPLPSVSGEATYLFYMGYIRLIHFSAGMVFTVVLLMRIYW
AFVGNRYSRELFIVPVWRKSWWQGVWYEIRWYLFLAKRPSADIGHNPIAQAAMFGYFLMSVFMIITGFALYSEHSQYAIF
APFRYVVEFFYWTGGNSMDIHSWHRLGMWLIGAFVIGHVYMALREDIMSDDTVISTMVNGYRSHKFGKISNKERS
>Mature_235_residues
MQQKSDNVVSHYVFEAPVRIWHWLTVLCMAVLMVTGYFIGKPLPSVSGEATYLFYMGYIRLIHFSAGMVFTVVLLMRIYW
AFVGNRYSRELFIVPVWRKSWWQGVWYEIRWYLFLAKRPSADIGHNPIAQAAMFGYFLMSVFMIITGFALYSEHSQYAIF
APFRYVVEFFYWTGGNSMDIHSWHRLGMWLIGAFVIGHVYMALREDIMSDDTVISTMVNGYRSHKFGKISNKERS

Specific function: Probable b-type cytochrome

COG id: COG1969

COG function: function code C; Ni,Fe-hydrogenase I cytochrome b subunit

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hupC/hyaC/hydC family

Homologues:

Organism=Escherichia coli, GI1787208, Length=235, Percent_Identity=100, Blast_Score=483, Evalue=1e-138,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): CYBH_ECO57 (P0AAM2)

Other databases:

- EMBL:   AE005174
- EMBL:   BA000007
- PIR:   B90770
- PIR:   F85632
- RefSeq:   NP_286911.1
- RefSeq:   NP_309157.1
- ProteinModelPortal:   P0AAM2
- SMR:   P0AAM2
- EnsemblBacteria:   EBESCT00000027121
- EnsemblBacteria:   EBESCT00000057573
- GeneID:   912637
- GeneID:   959037
- GenomeReviews:   AE005174_GR
- GenomeReviews:   BA000007_GR
- KEGG:   ece:Z1391
- KEGG:   ecs:ECs1130
- GeneTree:   EBGT00050000009999
- HOGENOM:   HBG484117
- OMA:   YMAIFNA
- ProtClustDB:   PRK10171
- BioCyc:   ECOL83334:ECS1130-MONOMER
- GO:   GO:0006810
- InterPro:   IPR005797
- InterPro:   IPR011577
- InterPro:   IPR016174
- InterPro:   IPR000516
- PRINTS:   PR00161
- TIGRFAMs:   TIGR02125

Pfam domain/function: PF00033 Cytochrom_B_N; PF01292 Ni_hydr_CYTB; SSF81342 Transmembr_di-haem_cytochrome

EC number: NA

Molecular weight: Translated: 27598; Mature: 27598

Theoretical pI: Translated: 9.59; Mature: 9.59

Prosite motif: PS00882 NI_HGENASE_CYTB_1; PS00883 NI_HGENASE_CYTB_2

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x13e89c44)-; HASH(0x139b9e60)-; HASH(0x1367b6bc)-; HASH(0x13618ee8)-;

Cys/Met content:

0.4 %Cys     (Translated Protein)
6.0 %Met     (Translated Protein)
6.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
6.0 %Met     (Mature Protein)
6.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQQKSDNVVSHYVFEAPVRIWHWLTVLCMAVLMVTGYFIGKPLPSVSGEATYLFYMGYIR
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHH
LIHFSAGMVFTVVLLMRIYWAFVGNRYSRELFIVPVWRKSWWQGVWYEIRWYLFLAKRPS
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCHHHHHHHHHHHEEEEEEEECCCC
ADIGHNPIAQAAMFGYFLMSVFMIITGFALYSEHSQYAIFAPFRYVVEFFYWTGGNSMDI
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHCCCCCCCH
HSWHRLGMWLIGAFVIGHVYMALREDIMSDDTVISTMVNGYRSHKFGKISNKERS
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCH
>Mature Secondary Structure
MQQKSDNVVSHYVFEAPVRIWHWLTVLCMAVLMVTGYFIGKPLPSVSGEATYLFYMGYIR
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHH
LIHFSAGMVFTVVLLMRIYWAFVGNRYSRELFIVPVWRKSWWQGVWYEIRWYLFLAKRPS
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCHHHHHHHHHHHEEEEEEEECCCC
ADIGHNPIAQAAMFGYFLMSVFMIITGFALYSEHSQYAIFAPFRYVVEFFYWTGGNSMDI
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHCCCCCCCH
HSWHRLGMWLIGAFVIGHVYMALREDIMSDDTVISTMVNGYRSHKFGKISNKERS
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCH

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Reduced ferredoxin; 2 H+ [C]

Specific reaction: Reduced ferredoxin + 2 H+ = 2 oxidized ferredoxin + H2 [C]

General reaction: Oxidoreductases [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796