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The map label for this gene is hyi [H]

Identifier: 157160031

GI number: 157160031

Start: 595287

End: 596063

Strand: Direct

Name: hyi [H]

Synonym: EcHS_A0582

Alternate gene names: 157160031

Gene position: 595287-596063 (Clockwise)

Preceding gene: 157160030

Following gene: 157160032

Centisome position: 12.82

GC content: 45.43

Gene sequence:

>777_bases
ATGTTACGTTTCTCTGCTAATTTATCGATGTTATTTGGAGAATATGATTTTCTCGCCCGTTTTGAGAAAGCTGCGCAGTG
TGGTTTTCGCGGCGTTGAATTTATGTTTCCTTATGACTACGACATTGAAGAATTAAAACAGGTGCTGGCGAGTAATAAAC
TCGAACATACGCTGCACAATCTACCGGCGGGTGACTGGGCGGCGGGGGAGCGCGGTATTGCCTGTATTCCTGGCCGTGAA
GAAGAGTTTCGGGATGGCGTAGCAGCAGCGATTCGTTATGCCCGTGCGCTGGGGAATAAAAAAATTAACTGTCTGGTCGG
TAAAACGCCGGCTGGTTTCAGCAGTGAACAGATTCACGCAACGCTTGTAGAAAACCTGCGTTATGCCGCGAATATGCTGA
TGAAAGAAGATATTTTATTACTGATTGAACCTATTAACCATTTTGATATTCCTGGTTTCCATCTCACCGGAACTCGGCAG
GCGCTGAAATTGATTGATGATGTTGGTTGCTGCAATTTGAAAATTCAGTATGACATTTATCACATGCAGCGGATGGAAGG
TGAATTAACCAACACCATGACTCAGTGGGCCGATAAAATTGGCCACCTGCAAATTGCTGATAATCCGCATCGCGGCGAAC
CGGGGACCGGAGAAATTAATTATGATTATCTCTTTAAGGTAATCGAAAATTCTGACTACAACGGTTGGGTTGGGTGTGAA
TATAAACCCCAAACCACCACGGAAGCCGGTTTACGCTGGATGGATCCGTACCGTTAA

Upstream 100 bases:

>100_bases
CGAACTGGATAACGTCATGGAATTTGAAGATATCGCCGATAACGCAGCGGACGCACCGACTGAAACCTGCTTCATGCACT
ATGAATAAGGGAGATAAATA

Downstream 100 bases:

>100_bases
AACGTAACGCTGTTCAGGCAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCTGAATTCAGGAAAAGCGA
AATTTAAAAGAGGTTAATTA

Product: hydroxypyruvate isomerase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 258; Mature: 258

Protein sequence:

>258_residues
MLRFSANLSMLFGEYDFLARFEKAAQCGFRGVEFMFPYDYDIEELKQVLASNKLEHTLHNLPAGDWAAGERGIACIPGRE
EEFRDGVAAAIRYARALGNKKINCLVGKTPAGFSSEQIHATLVENLRYAANMLMKEDILLLIEPINHFDIPGFHLTGTRQ
ALKLIDDVGCCNLKIQYDIYHMQRMEGELTNTMTQWADKIGHLQIADNPHRGEPGTGEINYDYLFKVIENSDYNGWVGCE
YKPQTTTEAGLRWMDPYR

Sequences:

>Translated_258_residues
MLRFSANLSMLFGEYDFLARFEKAAQCGFRGVEFMFPYDYDIEELKQVLASNKLEHTLHNLPAGDWAAGERGIACIPGRE
EEFRDGVAAAIRYARALGNKKINCLVGKTPAGFSSEQIHATLVENLRYAANMLMKEDILLLIEPINHFDIPGFHLTGTRQ
ALKLIDDVGCCNLKIQYDIYHMQRMEGELTNTMTQWADKIGHLQIADNPHRGEPGTGEINYDYLFKVIENSDYNGWVGCE
YKPQTTTEAGLRWMDPYR
>Mature_258_residues
MLRFSANLSMLFGEYDFLARFEKAAQCGFRGVEFMFPYDYDIEELKQVLASNKLEHTLHNLPAGDWAAGERGIACIPGRE
EEFRDGVAAAIRYARALGNKKINCLVGKTPAGFSSEQIHATLVENLRYAANMLMKEDILLLIEPINHFDIPGFHLTGTRQ
ALKLIDDVGCCNLKIQYDIYHMQRMEGELTNTMTQWADKIGHLQIADNPHRGEPGTGEINYDYLFKVIENSDYNGWVGCE
YKPQTTTEAGLRWMDPYR

Specific function: Catalyzes the reversible isomerization between hydroxypyruvate and 2-hydroxy-3-oxopropanoate (also termed tartronate semialdehyde) [H]

COG id: COG3622

COG function: function code G; Hydroxypyruvate isomerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hyi family [H]

Homologues:

Organism=Homo sapiens, GI300068962, Length=263, Percent_Identity=41.8250950570342, Blast_Score=204, Evalue=7e-53,
Organism=Homo sapiens, GI300068964, Length=244, Percent_Identity=41.3934426229508, Blast_Score=184, Evalue=6e-47,
Organism=Escherichia coli, GI1786718, Length=258, Percent_Identity=99.6124031007752, Blast_Score=540, Evalue=1e-155,
Organism=Escherichia coli, GI1789095, Length=258, Percent_Identity=40.3100775193798, Blast_Score=214, Evalue=5e-57,
Organism=Caenorhabditis elegans, GI17552022, Length=240, Percent_Identity=38.3333333333333, Blast_Score=175, Evalue=2e-44,
Organism=Drosophila melanogaster, GI17530883, Length=258, Percent_Identity=34.8837209302326, Blast_Score=162, Evalue=2e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017643
- InterPro:   IPR013022
- InterPro:   IPR012307 [H]

Pfam domain/function: PF01261 AP_endonuc_2 [H]

EC number: =5.3.1.22 [H]

Molecular weight: Translated: 29369; Mature: 29369

Theoretical pI: Translated: 5.18; Mature: 5.18

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLRFSANLSMLFGEYDFLARFEKAAQCGFRGVEFMFPYDYDIEELKQVLASNKLEHTLHN
CEEECCCCEEECCCHHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHHCCHHHHHHHH
LPAGDWAAGERGIACIPGREEEFRDGVAAAIRYARALGNKKINCLVGKTPAGFSSEQIHA
CCCCCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHH
TLVENLRYAANMLMKEDILLLIEPINHFDIPGFHLTGTRQALKLIDDVGCCNLKIQYDIY
HHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCEEECCHHHHHHHHHCCCCEEEEEEEEHH
HMQRMEGELTNTMTQWADKIGHLQIADNPHRGEPGTGEINYDYLFKVIENSDYNGWVGCE
HHHHHHHHHHHHHHHHHHHHCCEEECCCCCCCCCCCCCCCHHHHHHEECCCCCCCEECCE
YKPQTTTEAGLRWMDPYR
ECCCCCHHHCCEECCCCC
>Mature Secondary Structure
MLRFSANLSMLFGEYDFLARFEKAAQCGFRGVEFMFPYDYDIEELKQVLASNKLEHTLHN
CEEECCCCEEECCCHHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHHCCHHHHHHHH
LPAGDWAAGERGIACIPGREEEFRDGVAAAIRYARALGNKKINCLVGKTPAGFSSEQIHA
CCCCCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHH
TLVENLRYAANMLMKEDILLLIEPINHFDIPGFHLTGTRQALKLIDDVGCCNLKIQYDIY
HHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCEEECCHHHHHHHHHCCCCEEEEEEEEHH
HMQRMEGELTNTMTQWADKIGHLQIADNPHRGEPGTGEINYDYLFKVIENSDYNGWVGCE
HHHHHHHHHHHHHHHHHHHHCCEEECCCCCCCCCCCCCCCHHHHHHEECCCCCCCEECCE
YKPQTTTEAGLRWMDPYR
ECCCCCHHHCCEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8440684; 10561547; 9278503 [H]