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The map label for this gene is gcl [H]

Identifier: 157160030

GI number: 157160030

Start: 593493

End: 595274

Strand: Direct

Name: gcl [H]

Synonym: EcHS_A0581

Alternate gene names: 157160030

Gene position: 593493-595274 (Clockwise)

Preceding gene: 157160029

Following gene: 157160031

Centisome position: 12.78

GC content: 53.09

Gene sequence:

>1782_bases
ATGGCAAAAATGAGAGCCGTTGACGCGGCAATGTATGTGCTGGAGAAAGAAGGTATCACCACCGCCTTCGGTGTTCCGGG
AGCTGCAATCAATCCGTTCTACTCAGCGATGCGTAAGCACGGCGGTATTCGTCACATTCTGGCGCGTCATGTGGAAGGTG
CTTCGCACATGGCGGAAGGTTATACCCGCGCAACGGCAGGAAATATCGGCGTATGTCTGGGGACTTCCGGTCCTGCGGGC
ACGGACATGATCACCGCGCTCTATTCCGCTTCTGCTGATTCCATTCCTATTCTGTGCATTACCGGCCAGGCACCGCGCGC
CCGTCTGCATAAAGAAGATTTTCAGGCCGTAGATATTGAAGCAATTGCTAAACCGGTCAGCAAAATGGCGGTTACAGTTC
GTGAAGCGGCGCTGGTGCCTCGCGTGCTGCAACAGGCATTTCACCTGATGCGTTCTGGTCGTCCGGGTCCGGTACTGGTG
GATTTACCGTTCGACGTTCAGGTTGCGGAAATCGAGTTTGATCCTGACATGTACGAACCGCTGCCGGTCTACAAACCTGC
TGCCAGCCGTATGCAGATCGAAAAAGCTGTAGAAATGTTAATCCAGGCCGAACGTCCGGTGATTGTTGCCGGGGGCGGGG
TAATTAATGCTGACGCAGCTGCACTGTTACAACAGTTTGCTGAACTGACCAGCGTTCCGGTGATCCCAACGCTGATGGGC
TGGGGCTGTATCCCGGACGATCATGAACTGATGGCCGGGATGGTGGGTCTGCAAACCGCGCATCGTTACGGTAACGCAAC
GTTGCTGGCGTCCGACATGGTGTTTGGTATCGGTAACCGTTTTGCTAACCGTCATACCGGTTCGGTAGAGAAATACACCG
AAGGGCGCAAAATCGTTCATATCGATATTGAGCCGACGCAAATTGGCCGCGTGCTGTGTCCGGATCTGGGGATTGTCTCT
GATGCTAAAGCGGCGCTGACACTGCTGGTTGAAGTGGCGCAGGAAATGCAAAAAGCAGGGCGTCTGCCATGCCGTAAAGA
GTGGGTTGCTGAGTGCCAGCAGCGCAAACGTACTTTGTTGCGTAAAACACACTTCGACAACGTGCCGGTGAAACCGCAGC
GCGTGTATGAAGAGATGAACAAAGCTTTTGGACGTGATGTTTGCTATGTCACCACCATTGGTCTGTCGCAAATTGCCGCT
GCGCAAATGCTGCATGTCTTTAAAGACCGCCACTGGATCAACTGTGGTCAGGCTGGTCCGTTAGGCTGGACGATTCCGGC
TGCGCTAGGGGTTTGTGCCGCTGATCCGAAACGCAATGTGGTGGCGATTTCTGGCGACTTTGACTTCCAGTTCCTGATTG
AAGAGTTAGCCGTTGGCGCGCAGTTCAAAATTCCGTACATCCATGTACTGGTCAATAACGCTTATCTGGGGCTGATTCGC
CAGTCGCAGCGCGCGTTTGATATGGACTACTGCGTGCAACTCGCTTTCGAGAATATCAACTCCAGCGAAGTGAACGGTTA
CGGCGTCGACCACGTAAAAGTAGCGGAAGGTTTAGGTTGTAAAGCGATTCGCGTCTTCAAACCGGAAGATATTGCGCCAG
CCTTTGAACAGGCGAAAGCCTTAATGGCGCAATATCGGGTACCGGTAGTCGTGGAAGTTATTCTCGAGCGTGTGACCAAT
ATTTCGATGGGCAGCGAACTGGATAACGTCATGGAATTTGAAGATATCGCCGATAACGCAGCGGACGCACCGACTGAAAC
CTGCTTCATGCACTATGAATAA

Upstream 100 bases:

>100_bases
CACATCCATAATGTCTGTCGCATCCCGCTCTGCGGAGCGGGTTTTTTTGACAAAATTTGAAAGTTGGAAAAATTTTCCAA
TAAATAGAGGTAGGAATAAA

Downstream 100 bases:

>100_bases
GGGAGATAAATAATGTTACGTTTCTCTGCTAATTTATCGATGTTATTTGGAGAATATGATTTTCTCGCCCGTTTTGAGAA
AGCTGCGCAGTGTGGTTTTC

Product: glyoxylate carboligase

Products: NA

Alternate protein names: Tartronate-semialdehyde synthase [H]

Number of amino acids: Translated: 593; Mature: 592

Protein sequence:

>593_residues
MAKMRAVDAAMYVLEKEGITTAFGVPGAAINPFYSAMRKHGGIRHILARHVEGASHMAEGYTRATAGNIGVCLGTSGPAG
TDMITALYSASADSIPILCITGQAPRARLHKEDFQAVDIEAIAKPVSKMAVTVREAALVPRVLQQAFHLMRSGRPGPVLV
DLPFDVQVAEIEFDPDMYEPLPVYKPAASRMQIEKAVEMLIQAERPVIVAGGGVINADAAALLQQFAELTSVPVIPTLMG
WGCIPDDHELMAGMVGLQTAHRYGNATLLASDMVFGIGNRFANRHTGSVEKYTEGRKIVHIDIEPTQIGRVLCPDLGIVS
DAKAALTLLVEVAQEMQKAGRLPCRKEWVAECQQRKRTLLRKTHFDNVPVKPQRVYEEMNKAFGRDVCYVTTIGLSQIAA
AQMLHVFKDRHWINCGQAGPLGWTIPAALGVCAADPKRNVVAISGDFDFQFLIEELAVGAQFKIPYIHVLVNNAYLGLIR
QSQRAFDMDYCVQLAFENINSSEVNGYGVDHVKVAEGLGCKAIRVFKPEDIAPAFEQAKALMAQYRVPVVVEVILERVTN
ISMGSELDNVMEFEDIADNAADAPTETCFMHYE

Sequences:

>Translated_593_residues
MAKMRAVDAAMYVLEKEGITTAFGVPGAAINPFYSAMRKHGGIRHILARHVEGASHMAEGYTRATAGNIGVCLGTSGPAG
TDMITALYSASADSIPILCITGQAPRARLHKEDFQAVDIEAIAKPVSKMAVTVREAALVPRVLQQAFHLMRSGRPGPVLV
DLPFDVQVAEIEFDPDMYEPLPVYKPAASRMQIEKAVEMLIQAERPVIVAGGGVINADAAALLQQFAELTSVPVIPTLMG
WGCIPDDHELMAGMVGLQTAHRYGNATLLASDMVFGIGNRFANRHTGSVEKYTEGRKIVHIDIEPTQIGRVLCPDLGIVS
DAKAALTLLVEVAQEMQKAGRLPCRKEWVAECQQRKRTLLRKTHFDNVPVKPQRVYEEMNKAFGRDVCYVTTIGLSQIAA
AQMLHVFKDRHWINCGQAGPLGWTIPAALGVCAADPKRNVVAISGDFDFQFLIEELAVGAQFKIPYIHVLVNNAYLGLIR
QSQRAFDMDYCVQLAFENINSSEVNGYGVDHVKVAEGLGCKAIRVFKPEDIAPAFEQAKALMAQYRVPVVVEVILERVTN
ISMGSELDNVMEFEDIADNAADAPTETCFMHYE
>Mature_592_residues
AKMRAVDAAMYVLEKEGITTAFGVPGAAINPFYSAMRKHGGIRHILARHVEGASHMAEGYTRATAGNIGVCLGTSGPAGT
DMITALYSASADSIPILCITGQAPRARLHKEDFQAVDIEAIAKPVSKMAVTVREAALVPRVLQQAFHLMRSGRPGPVLVD
LPFDVQVAEIEFDPDMYEPLPVYKPAASRMQIEKAVEMLIQAERPVIVAGGGVINADAAALLQQFAELTSVPVIPTLMGW
GCIPDDHELMAGMVGLQTAHRYGNATLLASDMVFGIGNRFANRHTGSVEKYTEGRKIVHIDIEPTQIGRVLCPDLGIVSD
AKAALTLLVEVAQEMQKAGRLPCRKEWVAECQQRKRTLLRKTHFDNVPVKPQRVYEEMNKAFGRDVCYVTTIGLSQIAAA
QMLHVFKDRHWINCGQAGPLGWTIPAALGVCAADPKRNVVAISGDFDFQFLIEELAVGAQFKIPYIHVLVNNAYLGLIRQ
SQRAFDMDYCVQLAFENINSSEVNGYGVDHVKVAEGLGCKAIRVFKPEDIAPAFEQAKALMAQYRVPVVVEVILERVTNI
SMGSELDNVMEFEDIADNAADAPTETCFMHYE

Specific function: Catalyzes the condensation of two molecules of glyoxylate to give 2-hydroxy-3-oxopropanoate (also termed tartronate semialdehyde) [H]

COG id: COG3960

COG function: function code R; Glyoxylate carboligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=570, Percent_Identity=22.8070175438596, Blast_Score=114, Evalue=3e-25,
Organism=Homo sapiens, GI21361361, Length=601, Percent_Identity=24.7920133111481, Blast_Score=110, Evalue=3e-24,
Organism=Escherichia coli, GI1786717, Length=593, Percent_Identity=99.6627318718381, Blast_Score=1223, Evalue=0.0,
Organism=Escherichia coli, GI87081685, Length=568, Percent_Identity=33.6267605633803, Blast_Score=315, Evalue=6e-87,
Organism=Escherichia coli, GI1790104, Length=482, Percent_Identity=35.2697095435685, Blast_Score=279, Evalue=3e-76,
Organism=Escherichia coli, GI1787096, Length=548, Percent_Identity=25.1824817518248, Blast_Score=145, Evalue=6e-36,
Organism=Escherichia coli, GI1788716, Length=474, Percent_Identity=25.7383966244726, Blast_Score=132, Evalue=5e-32,
Organism=Caenorhabditis elegans, GI17531299, Length=494, Percent_Identity=24.4939271255061, Blast_Score=117, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI17531301, Length=495, Percent_Identity=25.2525252525253, Blast_Score=117, Evalue=3e-26,
Organism=Caenorhabditis elegans, GI17542570, Length=543, Percent_Identity=25.414364640884, Blast_Score=103, Evalue=3e-22,
Organism=Saccharomyces cerevisiae, GI6323755, Length=594, Percent_Identity=31.3131313131313, Blast_Score=265, Evalue=2e-71,
Organism=Saccharomyces cerevisiae, GI6320816, Length=563, Percent_Identity=23.6234458259325, Blast_Score=102, Evalue=2e-22,
Organism=Saccharomyces cerevisiae, GI6323163, Length=505, Percent_Identity=23.1683168316832, Blast_Score=80, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6321524, Length=484, Percent_Identity=21.900826446281, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI19922626, Length=487, Percent_Identity=24.435318275154, Blast_Score=137, Evalue=2e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006397
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =4.1.1.47 [H]

Molecular weight: Translated: 64761; Mature: 64629

Theoretical pI: Translated: 6.10; Mature: 6.10

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKMRAVDAAMYVLEKEGITTAFGVPGAAINPFYSAMRKHGGIRHILARHVEGASHMAEG
CCCHHHHHHHHHHHHHCCCEEECCCCCCHHHHHHHHHHHCCCHHHHHHHHHCCHHHHHHC
YTRATAGNIGVCLGTSGPAGTDMITALYSASADSIPILCITGQAPRARLHKEDFQAVDIE
HHHCCCCCEEEEEECCCCCCHHHHHHHHHCCCCCCEEEEEECCCCHHHHCHHCCCEECHH
AIAKPVSKMAVTVREAALVPRVLQQAFHLMRSGRPGPVLVDLPFDVQVAEIEFDPDMYEP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCEEEEEEEECCCCCCC
LPVYKPAASRMQIEKAVEMLIQAERPVIVAGGGVINADAAALLQQFAELTSVPVIPTLMG
CCCCCCCHHHHHHHHHHHHHHHCCCCEEEECCCEECHHHHHHHHHHHHHHCCCCHHHHHC
WGCIPDDHELMAGMVGLQTAHRYGNATLLASDMVFGIGNRFANRHTGSVEKYTEGRKIVH
CCCCCCHHHHHHHHHHHHHHHHCCCEEEEEHHHHHHHHHHHCCCCCCCCHHHCCCCEEEE
IDIEPTQIGRVLCPDLGIVSDAKAALTLLVEVAQEMQKAGRLPCRKEWVAECQQRKRTLL
EECCHHHHCHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
RKTHFDNVPVKPQRVYEEMNKAFGRDVCYVTTIGLSQIAAAQMLHVFKDRHWINCGQAGP
HHHCCCCCCCCHHHHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHHHHCCCEECCCCCCC
LGWTIPAALGVCAADPKRNVVAISGDFDFQFLIEELAVGAQFKIPYIHVLVNNAYLGLIR
CCEEHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHCCCEECCCEEEEEECCHHHHHHH
QSQRAFDMDYCVQLAFENINSSEVNGYGVDHVKVAEGLGCKAIRVFKPEDIAPAFEQAKA
HHHHHCCHHHHHHHHHHCCCCCCCCCCCCCCEEHHCCCCCCEEEEECCCCCCHHHHHHHH
LMAQYRVPVVVEVILERVTNISMGSELDNVMEFEDIADNAADAPTETCFMHYE
HHHHCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCCEEEECC
>Mature Secondary Structure 
AKMRAVDAAMYVLEKEGITTAFGVPGAAINPFYSAMRKHGGIRHILARHVEGASHMAEG
CCHHHHHHHHHHHHHCCCEEECCCCCCHHHHHHHHHHHCCCHHHHHHHHHCCHHHHHHC
YTRATAGNIGVCLGTSGPAGTDMITALYSASADSIPILCITGQAPRARLHKEDFQAVDIE
HHHCCCCCEEEEEECCCCCCHHHHHHHHHCCCCCCEEEEEECCCCHHHHCHHCCCEECHH
AIAKPVSKMAVTVREAALVPRVLQQAFHLMRSGRPGPVLVDLPFDVQVAEIEFDPDMYEP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCEEEEEEEECCCCCCC
LPVYKPAASRMQIEKAVEMLIQAERPVIVAGGGVINADAAALLQQFAELTSVPVIPTLMG
CCCCCCCHHHHHHHHHHHHHHHCCCCEEEECCCEECHHHHHHHHHHHHHHCCCCHHHHHC
WGCIPDDHELMAGMVGLQTAHRYGNATLLASDMVFGIGNRFANRHTGSVEKYTEGRKIVH
CCCCCCHHHHHHHHHHHHHHHHCCCEEEEEHHHHHHHHHHHCCCCCCCCHHHCCCCEEEE
IDIEPTQIGRVLCPDLGIVSDAKAALTLLVEVAQEMQKAGRLPCRKEWVAECQQRKRTLL
EECCHHHHCHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
RKTHFDNVPVKPQRVYEEMNKAFGRDVCYVTTIGLSQIAAAQMLHVFKDRHWINCGQAGP
HHHCCCCCCCCHHHHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHHHHCCCEECCCCCCC
LGWTIPAALGVCAADPKRNVVAISGDFDFQFLIEELAVGAQFKIPYIHVLVNNAYLGLIR
CCEEHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHCCCEECCCEEEEEECCHHHHHHH
QSQRAFDMDYCVQLAFENINSSEVNGYGVDHVKVAEGLGCKAIRVFKPEDIAPAFEQAKA
HHHHHCCHHHHHHHHHHCCCCCCCCCCCCCCEEHHCCCCCCEEEEECCCCCCHHHHHHHH
LMAQYRVPVVVEVILERVTNISMGSELDNVMEFEDIADNAADAPTETCFMHYE
HHHHCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]