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The map label for this gene is glxR [H]

Identifier: 157160032

GI number: 157160032

Start: 596163

End: 597041

Strand: Direct

Name: glxR [H]

Synonym: EcHS_A0583

Alternate gene names: 157160032

Gene position: 596163-597041 (Clockwise)

Preceding gene: 157160031

Following gene: 157160033

Centisome position: 12.84

GC content: 50.97

Gene sequence:

>879_bases
ATGAAACTGGGATTTATTGGCTTAGGCATTATGGGTACACCGATGGCCATTAATCTGGCGCGTGCCGGTCATCAACTGCA
TGTCACAGCCATTGGTCCGGTTGCTGATGAATTACTGTCACTGGGTGCCGTCAGTGTTGAAACTGCGCGCCAGGTAACGG
AAGCATCGGACATCATTTTTATTATGGTGCCAGATACACCACAGGTTGAAGAAGTTCTGTTCGGTGAAAATGGTTGTACC
AAAGCCTCGCTGAAGGGCAAAACCATTGTTGATATGAGCTCCATTTCCCCGATTGAAACTAAGCGTTTCGCTCGTCAGGT
GAATGAACTGGGCGGCGATTATCTCGATGCGCCAGTCTCCGGCGGTGAAATCGGTGCGCGTGAAGGGACGTTGTCGATTA
TGGTTGGCGGTGATGAAGCGGTATTTGAACGTGTTAAACCGCTGTTTGAACTGCTCGGTAAAAATATCACCCTCGTGGGC
GGTAACGGCGACGGTCAAACCTGCAAAGTGGCAAATCAGATTATCGTGGCGCTCAATATTGAAGCGGTTTCTGAAGCCCT
GCTATTTGCTTCAAAAGCCGGTGCGGACCCGGTACGTGTGCGCCAGGCGCTGATGGGCGGCTTTGCTTCCTCACGTATTC
TGGAAGTTCACGGTGAGCGCATGATTAAACGCACTTTTAATCCAGGTTTCAAAATTGCGCTGCACCAGAAAGATCTCAAC
CTGGCACTGCAAAGTGCGAAAGCGCTAGCGCTGAACCTGCCAAACACTGCGACCTGCCAGGAGTTATTTAATACCTGCGC
GGCAAACGGTGGCAGCCAGTTGGATCACTCTGCGTTAGTGCAGGCGCTGGAATTAATGGCTAACCATAAACTGGCCTGA

Upstream 100 bases:

>100_bases
AAACGTAACGCTGTTCAGGCAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCTGAATTCAGGAAAAGCG
AAATTTAAAAGAGGTTAATT

Downstream 100 bases:

>100_bases
TACCCGCAATAAAAATGGCCGATATCAGAAAATGAATCGGCCAGCAATATTAAAAAAGAAAGCAGCCAAAGATGTTGCTT
CAGTATTAAAAATAATATTT

Product: 2-hydroxy-3-oxopropionate reductase

Products: NA

Alternate protein names: Tartronate semialdehyde reductase; TSAR [H]

Number of amino acids: Translated: 292; Mature: 292

Protein sequence:

>292_residues
MKLGFIGLGIMGTPMAINLARAGHQLHVTAIGPVADELLSLGAVSVETARQVTEASDIIFIMVPDTPQVEEVLFGENGCT
KASLKGKTIVDMSSISPIETKRFARQVNELGGDYLDAPVSGGEIGAREGTLSIMVGGDEAVFERVKPLFELLGKNITLVG
GNGDGQTCKVANQIIVALNIEAVSEALLFASKAGADPVRVRQALMGGFASSRILEVHGERMIKRTFNPGFKIALHQKDLN
LALQSAKALALNLPNTATCQELFNTCAANGGSQLDHSALVQALELMANHKLA

Sequences:

>Translated_292_residues
MKLGFIGLGIMGTPMAINLARAGHQLHVTAIGPVADELLSLGAVSVETARQVTEASDIIFIMVPDTPQVEEVLFGENGCT
KASLKGKTIVDMSSISPIETKRFARQVNELGGDYLDAPVSGGEIGAREGTLSIMVGGDEAVFERVKPLFELLGKNITLVG
GNGDGQTCKVANQIIVALNIEAVSEALLFASKAGADPVRVRQALMGGFASSRILEVHGERMIKRTFNPGFKIALHQKDLN
LALQSAKALALNLPNTATCQELFNTCAANGGSQLDHSALVQALELMANHKLA
>Mature_292_residues
MKLGFIGLGIMGTPMAINLARAGHQLHVTAIGPVADELLSLGAVSVETARQVTEASDIIFIMVPDTPQVEEVLFGENGCT
KASLKGKTIVDMSSISPIETKRFARQVNELGGDYLDAPVSGGEIGAREGTLSIMVGGDEAVFERVKPLFELLGKNITLVG
GNGDGQTCKVANQIIVALNIEAVSEALLFASKAGADPVRVRQALMGGFASSRILEVHGERMIKRTFNPGFKIALHQKDLN
LALQSAKALALNLPNTATCQELFNTCAANGGSQLDHSALVQALELMANHKLA

Specific function: Glyoxylate catabolism. [C]

COG id: COG2084

COG function: function code I; 3-hydroxyisobutyrate dehydrogenase and related beta-hydroxyacid dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 3-hydroxyisobutyrate dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI23308751, Length=300, Percent_Identity=32, Blast_Score=140, Evalue=1e-33,
Organism=Homo sapiens, GI40556376, Length=283, Percent_Identity=27.5618374558304, Blast_Score=110, Evalue=2e-24,
Organism=Escherichia coli, GI1786719, Length=292, Percent_Identity=99.6575342465753, Blast_Score=588, Evalue=1e-169,
Organism=Escherichia coli, GI145693186, Length=289, Percent_Identity=43.9446366782007, Blast_Score=239, Evalue=1e-64,
Organism=Escherichia coli, GI1790315, Length=289, Percent_Identity=31.1418685121107, Blast_Score=127, Evalue=1e-30,
Organism=Escherichia coli, GI1789092, Length=282, Percent_Identity=28.3687943262411, Blast_Score=104, Evalue=6e-24,
Organism=Caenorhabditis elegans, GI17557316, Length=257, Percent_Identity=33.0739299610895, Blast_Score=111, Evalue=5e-25,
Organism=Drosophila melanogaster, GI24655230, Length=300, Percent_Identity=32.6666666666667, Blast_Score=135, Evalue=3e-32,
Organism=Drosophila melanogaster, GI19922568, Length=300, Percent_Identity=32.6666666666667, Blast_Score=135, Evalue=3e-32,
Organism=Drosophila melanogaster, GI28574115, Length=283, Percent_Identity=29.6819787985866, Blast_Score=117, Evalue=8e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002204
- InterPro:   IPR015815
- InterPro:   IPR008927
- InterPro:   IPR006115
- InterPro:   IPR013328
- InterPro:   IPR016040
- InterPro:   IPR006183
- InterPro:   IPR006398 [H]

Pfam domain/function: PF03446 NAD_binding_2 [H]

EC number: =1.1.1.60 [H]

Molecular weight: Translated: 30771; Mature: 30771

Theoretical pI: Translated: 5.96; Mature: 5.96

Prosite motif: PS00895 3_HYDROXYISOBUT_DH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLGFIGLGIMGTPMAINLARAGHQLHVTAIGPVADELLSLGAVSVETARQVTEASDIIF
CCEEEEECCCCCCCCEEEEECCCCEEEEEEECHHHHHHHHHCCHHHHHHHHHHCCCCEEE
IMVPDTPQVEEVLFGENGCTKASLKGKTIVDMSSISPIETKRFARQVNELGGDYLDAPVS
EEECCCCCHHHHHCCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHCCCEECCCCC
GGEIGAREGTLSIMVGGDEAVFERVKPLFELLGKNITLVGGNGDGQTCKVANQIIVALNI
CCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHCCCEEEEECCCCCCEEEECCEEEEEEEH
EAVSEALLFASKAGADPVRVRQALMGGFASSRILEVHGERMIKRTFNPGFKIALHQKDLN
HHHHHHHHHHHCCCCCHHHHHHHHHCCHHHCHHHHHHHHHHHHHHCCCCEEEEEEECCHH
LALQSAKALALNLPNTATCQELFNTCAANGGSQLDHSALVQALELMANHKLA
HHHHHCCEEEEECCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MKLGFIGLGIMGTPMAINLARAGHQLHVTAIGPVADELLSLGAVSVETARQVTEASDIIF
CCEEEEECCCCCCCCEEEEECCCCEEEEEEECHHHHHHHHHCCHHHHHHHHHHCCCCEEE
IMVPDTPQVEEVLFGENGCTKASLKGKTIVDMSSISPIETKRFARQVNELGGDYLDAPVS
EEECCCCCHHHHHCCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHCCCEECCCCC
GGEIGAREGTLSIMVGGDEAVFERVKPLFELLGKNITLVGGNGDGQTCKVANQIIVALNI
CCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHCCCEEEEECCCCCCEEEECCEEEEEEEH
EAVSEALLFASKAGADPVRVRQALMGGFASSRILEVHGERMIKRTFNPGFKIALHQKDLN
HHHHHHHHHHHCCCCCHHHHHHHHHCCHHHCHHHHHHHHHHHHHHCCCCEEEEEEECCHH
LALQSAKALALNLPNTATCQELFNTCAANGGSQLDHSALVQALELMANHKLA
HHHHHCCEEEEECCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10601204; 9278503 [H]