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The map label for this gene is istA [H]

Identifier: 157157074

GI number: 157157074

Start: 2190882

End: 2192054

Strand: Reverse

Name: istA [H]

Synonym: EcE24377A_2228

Alternate gene names: 157157074

Gene position: 2192054-2190882 (Counterclockwise)

Preceding gene: 157155074

Following gene: 157158336

Centisome position: 44.02

GC content: 54.39

Gene sequence:

>1173_bases
ATGCTGAGCAGAGAGGATTTTTACATGATAAAGCAAATGCGCCAGCAAGGTGCGTATATTGTTGATATTGCCACTCAGGT
TGGTTGCTCTGAGCGGACCGTCAGACGGTACCTGAAATACCCGGAACCTCCGGCCAGAAAAACACGCCACAAAATGGCCA
AACTCAAGCCGTTCATGGACTATATAGATATGCGTCTGGCTGAGAACGTCTGGAACGGCGAGGTCATCCTGGCGGAAATC
AAAGCGATGGGTTATACCGGTGGCCGTTCCATGTTGCGCTACTACATCCAGCCCAAACGTAAGATGCGGCCATCGAAGAA
AACGGTTCGCTTCGAAACTCAGCCCGGTTACCAGCTACAGCATGACTGGGGAGAAGTTGAGGCTGAGGTTGCCGGGCAAC
GATGCAGGGTTAACTTCGCGGTTAATACGCTGGGGTTCTCACGTCGCTTCCATGTCTTCGCGGCACCAAAGCAGGATGCT
GAACATACCTATGAGTCACTGGTCCGCGCCTTTCGCTACTTCGGCGGTAGCGTGAAAACCGTGCTGGTCGATAACCAGAA
AGCCGCAGTGCTGAAAAATAACAACGGGAAGGTGGTGTTCAACTCCGGGTTCCTGCTGCTGGCCGATCACTATGGCTTCC
TGCCACGGGCCTGCCGTCCACGAAGGGCCAGAACCAAAGGTAAGGTTGAACGGATGGTGAAATACCTCAAGGAAAACTTC
TTCATCCGGTACCGCCGGTTCGACAGCTTCGCCCATGTTAACCAGCTACTGGAGCAGTGGATGGCTGACGTTGCTGACAG
GCGAGAACTTCGCCAGTTCAGGCAGACACCGGAACAGCGCTTCACGCAGGAACAGGAGCATCTGCATCCGCTGCCGGGTA
CGGACTTCGATACCAGCTATTTCGATATCCGTCATGTCTCCTGGGATGGCTATATCGAGGTTGGCGGGAACCGTTACAGC
GTACCGGAAAGCCTGTGTGGCCAGCCGGTATCAATACGGATCTCGCTGGATGATGAACTACGGATCTACAGTAATGAGCA
ACAGGTAGCATCGCACCGTCTCTGTTCGGCTGCATCCAGCTGGCAAACCGTGCCGGAGCATCACGCCCCACTCTGGCAGC
AGGTCAGTCAGGTGGAACACAGACCGTTGAGTACGTATGAGGAGCTGTTGTGA

Upstream 100 bases:

>100_bases
GTCAGCGCCAATGATATAAGACAGTAATTCACCATTTGGATTGTCCGCTCCACCCAACATGTTGTTTCCTTAAGGTTCCT
ACACCACAAAGGGAACACTC

Downstream 100 bases:

>100_bases
TGCATGAACTGGAGACACTGCTGAGTCGTCTTAAAATGGAACATCTGGGCTACCACGTTGAAAGCCTGCTGGAGCAGGCG
GCCAAAAAAGAACTGAACTA

Product: IS21 family transposase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 390; Mature: 390

Protein sequence:

>390_residues
MLSREDFYMIKQMRQQGAYIVDIATQVGCSERTVRRYLKYPEPPARKTRHKMAKLKPFMDYIDMRLAENVWNGEVILAEI
KAMGYTGGRSMLRYYIQPKRKMRPSKKTVRFETQPGYQLQHDWGEVEAEVAGQRCRVNFAVNTLGFSRRFHVFAAPKQDA
EHTYESLVRAFRYFGGSVKTVLVDNQKAAVLKNNNGKVVFNSGFLLLADHYGFLPRACRPRRARTKGKVERMVKYLKENF
FIRYRRFDSFAHVNQLLEQWMADVADRRELRQFRQTPEQRFTQEQEHLHPLPGTDFDTSYFDIRHVSWDGYIEVGGNRYS
VPESLCGQPVSIRISLDDELRIYSNEQQVASHRLCSAASSWQTVPEHHAPLWQQVSQVEHRPLSTYEELL

Sequences:

>Translated_390_residues
MLSREDFYMIKQMRQQGAYIVDIATQVGCSERTVRRYLKYPEPPARKTRHKMAKLKPFMDYIDMRLAENVWNGEVILAEI
KAMGYTGGRSMLRYYIQPKRKMRPSKKTVRFETQPGYQLQHDWGEVEAEVAGQRCRVNFAVNTLGFSRRFHVFAAPKQDA
EHTYESLVRAFRYFGGSVKTVLVDNQKAAVLKNNNGKVVFNSGFLLLADHYGFLPRACRPRRARTKGKVERMVKYLKENF
FIRYRRFDSFAHVNQLLEQWMADVADRRELRQFRQTPEQRFTQEQEHLHPLPGTDFDTSYFDIRHVSWDGYIEVGGNRYS
VPESLCGQPVSIRISLDDELRIYSNEQQVASHRLCSAASSWQTVPEHHAPLWQQVSQVEHRPLSTYEELL
>Mature_390_residues
MLSREDFYMIKQMRQQGAYIVDIATQVGCSERTVRRYLKYPEPPARKTRHKMAKLKPFMDYIDMRLAENVWNGEVILAEI
KAMGYTGGRSMLRYYIQPKRKMRPSKKTVRFETQPGYQLQHDWGEVEAEVAGQRCRVNFAVNTLGFSRRFHVFAAPKQDA
EHTYESLVRAFRYFGGSVKTVLVDNQKAAVLKNNNGKVVFNSGFLLLADHYGFLPRACRPRRARTKGKVERMVKYLKENF
FIRYRRFDSFAHVNQLLEQWMADVADRRELRQFRQTPEQRFTQEQEHLHPLPGTDFDTSYFDIRHVSWDGYIEVGGNRYS
VPESLCGQPVSIRISLDDELRIYSNEQQVASHRLCSAASSWQTVPEHHAPLWQQVSQVEHRPLSTYEELL

Specific function: Involved in the transposition of the insertion sequence [H]

COG id: COG4584

COG function: function code L; Transposase and inactivated derivatives

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 integrase catalytic domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017894
- InterPro:   IPR001584
- InterPro:   IPR012337 [H]

Pfam domain/function: PF00665 rve [H]

EC number: NA

Molecular weight: Translated: 45760; Mature: 45760

Theoretical pI: Translated: 9.87; Mature: 9.87

Prosite motif: PS50994 INTEGRASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSREDFYMIKQMRQQGAYIVDIATQVGCSERTVRRYLKYPEPPARKTRHKMAKLKPFMD
CCCCHHHHHHHHHHHCCCEEEEEHHHCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
YIDMRLAENVWNGEVILAEIKAMGYTGGRSMLRYYIQPKRKMRPSKKTVRFETQPGYQLQ
HHHHHHHHCCCCCCEEEEEHHHHCCCCHHHHHHHHHCCHHHCCCCCCCEEEECCCCCEEC
HDWGEVEAEVAGQRCRVNFAVNTLGFSRRFHVFAAPKQDAEHTYESLVRAFRYFGGSVKT
CCHHHHHHHHCCCEEEEEEEEECCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHCCCEEE
VLVDNQKAAVLKNNNGKVVFNSGFLLLADHYGFLPRACRPRRARTKGKVERMVKYLKENF
EEECCCCEEEEECCCCEEEEECCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
FIRYRRFDSFAHVNQLLEQWMADVADRRELRQFRQTPEQRFTQEQEHLHPLPGTDFDTSY
EEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCHHHHCCCCCCCCCCCCE
FDIRHVSWDGYIEVGGNRYSVPESLCGQPVSIRISLDDELRIYSNEQQVASHRLCSAASS
EEEEEECCCCEEEECCCEECCCHHHCCCCEEEEEEECCCEEEECCHHHHHHHHHHHHHHH
WQTVPEHHAPLWQQVSQVEHRPLSTYEELL
CCCCCHHCCHHHHHHHHHHCCCCHHHHHHC
>Mature Secondary Structure
MLSREDFYMIKQMRQQGAYIVDIATQVGCSERTVRRYLKYPEPPARKTRHKMAKLKPFMD
CCCCHHHHHHHHHHHCCCEEEEEHHHCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
YIDMRLAENVWNGEVILAEIKAMGYTGGRSMLRYYIQPKRKMRPSKKTVRFETQPGYQLQ
HHHHHHHHCCCCCCEEEEEHHHHCCCCHHHHHHHHHCCHHHCCCCCCCEEEECCCCCEEC
HDWGEVEAEVAGQRCRVNFAVNTLGFSRRFHVFAAPKQDAEHTYESLVRAFRYFGGSVKT
CCHHHHHHHHCCCEEEEEEEEECCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHCCCEEE
VLVDNQKAAVLKNNNGKVVFNSGFLLLADHYGFLPRACRPRRARTKGKVERMVKYLKENF
EEECCCCEEEEECCCCEEEEECCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
FIRYRRFDSFAHVNQLLEQWMADVADRRELRQFRQTPEQRFTQEQEHLHPLPGTDFDTSY
EEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCHHHHCCCCCCCCCCCCE
FDIRHVSWDGYIEVGGNRYSVPESLCGQPVSIRISLDDELRIYSNEQQVASHRLCSAASS
EEEEEECCCCEEEECCCEECCCHHHCCCCEEEEEEECCCEEEECCHHHHHHHHHHHHHHH
WQTVPEHHAPLWQQVSQVEHRPLSTYEELL
CCCCCHHCCHHHHHHHHHHCCCCHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2540414 [H]