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The map label for this gene is citE [H]

Identifier: 15675157

GI number: 15675157

Start: 973897

End: 974784

Strand: Direct

Name: citE [H]

Synonym: SPy_1188

Alternate gene names: 15675157

Gene position: 973897-974784 (Clockwise)

Preceding gene: 15675156

Following gene: 15675158

Centisome position: 52.57

GC content: 42.45

Gene sequence:

>888_bases
ATGGAACGTTTAAGAAGAACAATGATGTTTGTGCCTGGTGCCAATGCAGCCATGCTTCGAGATGCTCCTTTATTTGGCGC
TGATTCAATCATGTTTGACTTGGAAGATTCTGTTTCACTCAAAGAAAAAGACACCTCTAGAGCTCTCGTTCATTTTGCGC
TTAAAACTTTTGATTATTCAAGCGTTGAAACGGTTGTTCGTGTGAATGGTCTTGATTCTTGTGGGGCTTTAGACATCGAA
GCTGTTGTTTTGGCAGGTGTCAACGTGATTCGTCTTCCAAAAACAGAGACTGCTCAAGATATTATTGATGTGGAGGCTGT
TATTGAACGTGTCGAACGCGAGAACAGCATTGAAGTTGGTCGCACACGTATGATGGCAGCCATTGAATCAGCCGAAGGTG
TCCTAAATGCTCGTGAGATTGCCAAAGCTTCTAAGCGCTTGATTGGTATTGCTCTTGGAGCAGAAGACTATGTCACGAAT
ATGAAAACGCGTCGTTACCCAGATGGTCAAGAATTATTCTTTGCTCGTAGCATGATTTTACACGCTGCTCGTGCTGCTGG
AATTGCTGCCATTGATACTGTTTATTCTGATGTCAATAATACCGAAGGGTTCCAAAACGAAGTTCGCATGATCAAACAGT
TAGGATTTGATGGTAAATCGGTCATCAACCCTCGCCAAATTCCTCTGGTCAATGAGATTTATACCCCAACAAAAAAAGAA
ATTGACCATGCCAAACAAGTCATCTGGGCAATTCGTGAAGCTGAAAGCAAAGGCTCTGGCGTTATTTCCTTAAATGGAAA
AATGGTTGATAAACCAATCGTTGAACGTGCAGAACGTGTGATTGCCCTAGCAACAGCAGCAGGTGTTTTATCTGAGGAGG
ATATTTAA

Upstream 100 bases:

>100_bases
TTGATAAAGGTGCATTAGATTGCACCATTCAAGCGAGAACCATCGCAGCTGTTCATCGCGCTGCTGGTATTGACCAATAC
GATTGGAAGGAGATTGACTC

Downstream 100 bases:

>100_bases
AGATGGTAACAAATAAACTCGGTAGAGATATTCCTCAACCATACGCCGACCAATACGGTGTCTTTGAAGGAGAACTCGCA
AATATCAAACAGTATGACGA

Product: putative citrate lyase subunit beta

Products: NA

Alternate protein names: Citrase beta chain; Citrate (pro-3S)-lyase subunit beta; Citryl-CoA lyase subunit [H]

Number of amino acids: Translated: 295; Mature: 295

Protein sequence:

>295_residues
MERLRRTMMFVPGANAAMLRDAPLFGADSIMFDLEDSVSLKEKDTSRALVHFALKTFDYSSVETVVRVNGLDSCGALDIE
AVVLAGVNVIRLPKTETAQDIIDVEAVIERVERENSIEVGRTRMMAAIESAEGVLNAREIAKASKRLIGIALGAEDYVTN
MKTRRYPDGQELFFARSMILHAARAAGIAAIDTVYSDVNNTEGFQNEVRMIKQLGFDGKSVINPRQIPLVNEIYTPTKKE
IDHAKQVIWAIREAESKGSGVISLNGKMVDKPIVERAERVIALATAAGVLSEEDI

Sequences:

>Translated_295_residues
MERLRRTMMFVPGANAAMLRDAPLFGADSIMFDLEDSVSLKEKDTSRALVHFALKTFDYSSVETVVRVNGLDSCGALDIE
AVVLAGVNVIRLPKTETAQDIIDVEAVIERVERENSIEVGRTRMMAAIESAEGVLNAREIAKASKRLIGIALGAEDYVTN
MKTRRYPDGQELFFARSMILHAARAAGIAAIDTVYSDVNNTEGFQNEVRMIKQLGFDGKSVINPRQIPLVNEIYTPTKKE
IDHAKQVIWAIREAESKGSGVISLNGKMVDKPIVERAERVIALATAAGVLSEEDI
>Mature_295_residues
MERLRRTMMFVPGANAAMLRDAPLFGADSIMFDLEDSVSLKEKDTSRALVHFALKTFDYSSVETVVRVNGLDSCGALDIE
AVVLAGVNVIRLPKTETAQDIIDVEAVIERVERENSIEVGRTRMMAAIESAEGVLNAREIAKASKRLIGIALGAEDYVTN
MKTRRYPDGQELFFARSMILHAARAAGIAAIDTVYSDVNNTEGFQNEVRMIKQLGFDGKSVINPRQIPLVNEIYTPTKKE
IDHAKQVIWAIREAESKGSGVISLNGKMVDKPIVERAERVIALATAAGVLSEEDI

Specific function: Represents a citryl-ACP lyase [H]

COG id: COG2301

COG function: function code G; Citrate lyase beta subunit

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HpcH/HpaI aldolase family. Citrate lyase beta subunit subfamily [H]

Homologues:

Organism=Homo sapiens, GI45545437, Length=293, Percent_Identity=27.6450511945392, Blast_Score=129, Evalue=2e-30,
Organism=Escherichia coli, GI87081764, Length=291, Percent_Identity=57.7319587628866, Blast_Score=327, Evalue=5e-91,
Organism=Caenorhabditis elegans, GI17557458, Length=295, Percent_Identity=28.4745762711864, Blast_Score=139, Evalue=1e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005000
- InterPro:   IPR011206
- InterPro:   IPR006475
- InterPro:   IPR015813 [H]

Pfam domain/function: PF03328 HpcH_HpaI [H]

EC number: =4.1.3.6; =4.1.3.34 [H]

Molecular weight: Translated: 32397; Mature: 32397

Theoretical pI: Translated: 5.02; Mature: 5.02

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MERLRRTMMFVPGANAAMLRDAPLFGADSIMFDLEDSVSLKEKDTSRALVHFALKTFDYS
CHHHHHHEEECCCCCCHHEECCCCCCCCCEEEECCCCCCCCCCHHHHHHHHHHHHHCCCH
SVETVVRVNGLDSCGALDIEAVVLAGVNVIRLPKTETAQDIIDVEAVIERVERENSIEVG
HHHHEEEECCCCCCCCCCHHHHHHCCCCEEECCCCCHHHHHHHHHHHHHHHHCCCCHHHH
RTRMMAAIESAEGVLNAREIAKASKRLIGIALGAEDYVTNMKTRRYPDGQELFFARSMIL
HHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCHHHHHCCHHCCCCCHHHHHHHHHHHH
HAARAAGIAAIDTVYSDVNNTEGFQNEVRMIKQLGFDGKSVINPRQIPLVNEIYTPTKKE
HHHHHCCHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHCCCCHHH
IDHAKQVIWAIREAESKGSGVISLNGKMVDKPIVERAERVIALATAAGVLSEEDI
HHHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MERLRRTMMFVPGANAAMLRDAPLFGADSIMFDLEDSVSLKEKDTSRALVHFALKTFDYS
CHHHHHHEEECCCCCCHHEECCCCCCCCCEEEECCCCCCCCCCHHHHHHHHHHHHHCCCH
SVETVVRVNGLDSCGALDIEAVVLAGVNVIRLPKTETAQDIIDVEAVIERVERENSIEVG
HHHHEEEECCCCCCCCCCHHHHHHCCCCEEECCCCCHHHHHHHHHHHHHHHHCCCCHHHH
RTRMMAAIESAEGVLNAREIAKASKRLIGIALGAEDYVTNMKTRRYPDGQELFFARSMIL
HHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCHHHHHCCHHCCCCCHHHHHHHHHHHH
HAARAAGIAAIDTVYSDVNNTEGFQNEVRMIKQLGFDGKSVINPRQIPLVNEIYTPTKKE
HHHHHCCHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHCCCCHHH
IDHAKQVIWAIREAESKGSGVISLNGKMVDKPIVERAERVIALATAAGVLSEEDI
HHHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9457870 [H]