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The map label for this gene is citF [H]

Identifier: 15675158

GI number: 15675158

Start: 974787

End: 976319

Strand: Direct

Name: citF [H]

Synonym: SPy_1189

Alternate gene names: 15675158

Gene position: 974787-976319 (Clockwise)

Preceding gene: 15675157

Following gene: 15675159

Centisome position: 52.62

GC content: 42.66

Gene sequence:

>1533_bases
ATGGTAACAAATAAACTCGGTAGAGATATTCCTCAACCATACGCCGACCAATACGGTGTCTTTGAAGGAGAACTCGCAAA
TATCAAACAGTATGACGAATCAAGTCGCCGTATTAAACCAGTTAAGCCTGGAGATTCTAAATTACTAGGATCTGTTCGTG
AAGCGATTGAAAAAACAGGTTTAACAGATGGCATGACCATTTCTTTCCACCACCATTTCCGTGAAGGGGATTTCATTATG
AATATGGTCTTGGAGGAAATTGCCAAAATGGGCATTAAAAACCTGTCTATTGCCCCAAGTTCCATCGCTAATGTACATGA
GCCTTTGATTGATCACATCAAAAATGGTGTGGTCACTAACATCACTTCATCTGGTCTTCGTGACAAAGTAGGGGCAGCCA
TCTCAGAAGGACTAATGGAAAATCCTGTGGTTATTCGCTCCCATGGTGGTCGCGCTCGTGCCATTGCTAGTGGGGATATC
CATATTGATGTTGCCTTTCTGGGCGCCCCAAGTTCAGATGCTTACGGAAATGTTAATGGGACAAAAGGGAAAGCAACCTG
TGGTTCTTTGGGCTATGCCATGATTGATGCCAAATATGCGGATCAGGTGGTTATCTTGACAGATAATTTGGTTCCTTATC
CTAATACCCCAATCAGCATTCCTCAAACAGATGTTGACTATGTGGTAACAGTGGATGCTATCGGAGATCCTCAAGGAATC
GCCAAAGGGGCAACTCGTTTTACGAAAAATCCTAAAGAACTCTTAATTGCAGAATACGCCGCTAAAGTAATCACCAACTC
TCCTTATTTTAAAGAAGGATTCTCTTTCCAAACTGGAACAGGTGGCGCTTCTTTGGCAGTAACCCGTTTCATGCGTGAAG
CTATGATTAAAGAAAATATCAAAGCTAGCTTTGCTCTTGGTGGTATTACCAATGCTATGGTGGAGTTGCTCGAAGAAGAA
CTGGTTGAAAAAATTCTTGATGTCCAAGACTTTGATCACCCATCAGCTGTTTCTCTTGGTAAGCATGCTGAACATTATGA
AATTGATGCCAACATGTATGCCTCACCTTTGAGCAAGGGTGCTGTTATCAATCAATTAGACACTTGTATTTTATCAGCCC
TTGAAGTTGATACTAACTTCAATGTTAATGTGATGACAGGATCTGACGGCGTGATTCGTGGCGCTTCTGGAGGACACTGC
GACACTGCCTTTGCGGCTAAGATGAGTTTGGTTATTTCACCACTTATCCGCGGACGCATCCCAACTTTTGTAGATGAGGT
TAATACGGTCATTACACCAGGAACAAGTGTCGATGTGATCGTCACAGAAGTGGGAATTGCCATTAACCCAAATCGTCAAG
ACTTAGTAGACCATTTCAAATCCCTCAATGTGCCACAATTTAGTATTGAGGAGCTAAAAGAGAAGGCTTACGCCATCGTT
GGCACACCTGAGCGTATTCAATATGGTGATAAGGTTGTTGCTCTTATTGAATACCGTGACGGTAGCCTCATGGATGTGGT
TTACAATGTGTAA

Upstream 100 bases:

>100_bases
TAAATGGAAAAATGGTTGATAAACCAATCGTTGAACGTGCAGAACGTGTGATTGCCCTAGCAACAGCAGCAGGTGTTTTA
TCTGAGGAGGATATTTAAAG

Downstream 100 bases:

>100_bases
AGACACTTATTTTTCAGGCGAAGCTATCCAACTTAGTGATATGTTAAGAGCCCGCGAAGAAAGAGCTCTGCGTCAGCTGC
ATTTATTAAAGGAGTACCCA

Product: putative citrate lyase subunit alpha

Products: NA

Alternate protein names: Citrase alpha chain; Citrate (pro-3S)-lyase alpha chain; Citrate CoA-transferase subunit [H]

Number of amino acids: Translated: 510; Mature: 510

Protein sequence:

>510_residues
MVTNKLGRDIPQPYADQYGVFEGELANIKQYDESSRRIKPVKPGDSKLLGSVREAIEKTGLTDGMTISFHHHFREGDFIM
NMVLEEIAKMGIKNLSIAPSSIANVHEPLIDHIKNGVVTNITSSGLRDKVGAAISEGLMENPVVIRSHGGRARAIASGDI
HIDVAFLGAPSSDAYGNVNGTKGKATCGSLGYAMIDAKYADQVVILTDNLVPYPNTPISIPQTDVDYVVTVDAIGDPQGI
AKGATRFTKNPKELLIAEYAAKVITNSPYFKEGFSFQTGTGGASLAVTRFMREAMIKENIKASFALGGITNAMVELLEEE
LVEKILDVQDFDHPSAVSLGKHAEHYEIDANMYASPLSKGAVINQLDTCILSALEVDTNFNVNVMTGSDGVIRGASGGHC
DTAFAAKMSLVISPLIRGRIPTFVDEVNTVITPGTSVDVIVTEVGIAINPNRQDLVDHFKSLNVPQFSIEELKEKAYAIV
GTPERIQYGDKVVALIEYRDGSLMDVVYNV

Sequences:

>Translated_510_residues
MVTNKLGRDIPQPYADQYGVFEGELANIKQYDESSRRIKPVKPGDSKLLGSVREAIEKTGLTDGMTISFHHHFREGDFIM
NMVLEEIAKMGIKNLSIAPSSIANVHEPLIDHIKNGVVTNITSSGLRDKVGAAISEGLMENPVVIRSHGGRARAIASGDI
HIDVAFLGAPSSDAYGNVNGTKGKATCGSLGYAMIDAKYADQVVILTDNLVPYPNTPISIPQTDVDYVVTVDAIGDPQGI
AKGATRFTKNPKELLIAEYAAKVITNSPYFKEGFSFQTGTGGASLAVTRFMREAMIKENIKASFALGGITNAMVELLEEE
LVEKILDVQDFDHPSAVSLGKHAEHYEIDANMYASPLSKGAVINQLDTCILSALEVDTNFNVNVMTGSDGVIRGASGGHC
DTAFAAKMSLVISPLIRGRIPTFVDEVNTVITPGTSVDVIVTEVGIAINPNRQDLVDHFKSLNVPQFSIEELKEKAYAIV
GTPERIQYGDKVVALIEYRDGSLMDVVYNV
>Mature_510_residues
MVTNKLGRDIPQPYADQYGVFEGELANIKQYDESSRRIKPVKPGDSKLLGSVREAIEKTGLTDGMTISFHHHFREGDFIM
NMVLEEIAKMGIKNLSIAPSSIANVHEPLIDHIKNGVVTNITSSGLRDKVGAAISEGLMENPVVIRSHGGRARAIASGDI
HIDVAFLGAPSSDAYGNVNGTKGKATCGSLGYAMIDAKYADQVVILTDNLVPYPNTPISIPQTDVDYVVTVDAIGDPQGI
AKGATRFTKNPKELLIAEYAAKVITNSPYFKEGFSFQTGTGGASLAVTRFMREAMIKENIKASFALGGITNAMVELLEEE
LVEKILDVQDFDHPSAVSLGKHAEHYEIDANMYASPLSKGAVINQLDTCILSALEVDTNFNVNVMTGSDGVIRGASGGHC
DTAFAAKMSLVISPLIRGRIPTFVDEVNTVITPGTSVDVIVTEVGIAINPNRQDLVDHFKSLNVPQFSIEELKEKAYAIV
GTPERIQYGDKVVALIEYRDGSLMDVVYNV

Specific function: Represents a citrate:acetyl-ACP transferase [H]

COG id: COG3051

COG function: function code C; Citrate lyase, alpha subunit

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1786832, Length=469, Percent_Identity=57.3560767590618, Blast_Score=566, Evalue=1e-162,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006472 [H]

Pfam domain/function: PF04223 CitF [H]

EC number: =4.1.3.6; =2.8.3.10 [H]

Molecular weight: Translated: 54991; Mature: 54991

Theoretical pI: Translated: 4.97; Mature: 4.97

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVTNKLGRDIPQPYADQYGVFEGELANIKQYDESSRRIKPVKPGDSKLLGSVREAIEKTG
CCCCCCCCCCCCCHHHHCCCCCCCHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHCC
LTDGMTISFHHHFREGDFIMNMVLEEIAKMGIKNLSIAPSSIANVHEPLIDHIKNGVVTN
CCCCEEEEEEECCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCEEEE
ITSSGLRDKVGAAISEGLMENPVVIRSHGGRARAIASGDIHIDVAFLGAPSSDAYGNVNG
CCCCCHHHHHHHHHHHHHHCCCEEEECCCCCEEEEECCCEEEEEEEEECCCCCCCCCCCC
TKGKATCGSLGYAMIDAKYADQVVILTDNLVPYPNTPISIPQTDVDYVVTVDAIGDPQGI
CCCCCCCCCCCHHEEEHHCCCEEEEEECCCCCCCCCCCCCCCCCCCEEEEEECCCCCHHH
AKGATRFTKNPKELLIAEYAAKVITNSPYFKEGFSFQTGTGGASLAVTRFMREAMIKENI
HHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCC
KASFALGGITNAMVELLEEELVEKILDVQDFDHPSAVSLGKHAEHYEIDANMYASPLSKG
HHEEECCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCCEEEECCCHHHCCCCCC
AVINQLDTCILSALEVDTNFNVNVMTGSDGVIRGASGGHCDTAFAAKMSLVISPLIRGRI
HHHHHHHHHHHHHHEECCCCEEEEEECCCCEEECCCCCCCHHHHHHHHHHHHHHHHHCCC
PTFVDEVNTVITPGTSVDVIVTEVGIAINPNRQDLVDHFKSLNVPQFSIEELKEKAYAIV
CHHHHCCCCEECCCCCEEEEEEEECEEECCCHHHHHHHHHHCCCCCHHHHHHHHHHEEEE
GTPERIQYGDKVVALIEYRDGSLMDVVYNV
CCHHHHCCCCEEEEEEEECCCCEEEHEECC
>Mature Secondary Structure
MVTNKLGRDIPQPYADQYGVFEGELANIKQYDESSRRIKPVKPGDSKLLGSVREAIEKTG
CCCCCCCCCCCCCHHHHCCCCCCCHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHCC
LTDGMTISFHHHFREGDFIMNMVLEEIAKMGIKNLSIAPSSIANVHEPLIDHIKNGVVTN
CCCCEEEEEEECCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCEEEE
ITSSGLRDKVGAAISEGLMENPVVIRSHGGRARAIASGDIHIDVAFLGAPSSDAYGNVNG
CCCCCHHHHHHHHHHHHHHCCCEEEECCCCCEEEEECCCEEEEEEEEECCCCCCCCCCCC
TKGKATCGSLGYAMIDAKYADQVVILTDNLVPYPNTPISIPQTDVDYVVTVDAIGDPQGI
CCCCCCCCCCCHHEEEHHCCCEEEEEECCCCCCCCCCCCCCCCCCCEEEEEECCCCCHHH
AKGATRFTKNPKELLIAEYAAKVITNSPYFKEGFSFQTGTGGASLAVTRFMREAMIKENI
HHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCC
KASFALGGITNAMVELLEEELVEKILDVQDFDHPSAVSLGKHAEHYEIDANMYASPLSKG
HHEEECCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCCEEEECCCHHHCCCCCC
AVINQLDTCILSALEVDTNFNVNVMTGSDGVIRGASGGHCDTAFAAKMSLVISPLIRGRI
HHHHHHHHHHHHHHEECCCCEEEEEECCCCEEECCCCCCCHHHHHHHHHHHHHHHHHCCC
PTFVDEVNTVITPGTSVDVIVTEVGIAINPNRQDLVDHFKSLNVPQFSIEELKEKAYAIV
CHHHHCCCCEECCCCCEEEEEEEECEEECCCHHHHHHHHHHCCCCCHHHHHHHHHHEEEE
GTPERIQYGDKVVALIEYRDGSLMDVVYNV
CCHHHHCCCCEEEEEEEECCCCEEEHEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]