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The map label for this gene is folK

Identifier: 15675083

GI number: 15675083

Start: 899776

End: 900276

Strand: Direct

Name: folK

Synonym: SPy_1100

Alternate gene names: 15675083

Gene position: 899776-900276 (Clockwise)

Preceding gene: 15675082

Following gene: 15675084

Centisome position: 48.57

GC content: 39.92

Gene sequence:

>501_bases
ATGACCATTGTTTATTTAAGTTTAGGCACCAATATGGGGGACCGAGCAGCTTACTTGCAAAAAGCTCTTGAGGCTTTAGC
TGACTTACCTCAAACACGGTTGCTTGCTCAATCATCCATTTATGAAACGACTGCTTGGGGCAAGACAGGCCAGGCTGATT
TTCTCAATATGGCCTGTCAATTAGATACACAATTAACCGCGGCTGATTTCTTAAAAGAAACACAAGCTATTGAGCAATCT
CTTGGTCGTGTGAGGCATGAAAAATGGGGATCAAGAACTATTGACATTGATATCTTGCTATTTGGAGAAGAGGTTTATGA
CACAAAGGAATTGAAAGTGCCACACCCTTATATGACTGAGCGTGCTTTTGTTTTAATCCCCTTATTGGAATTGCAGCCAG
ATTTAAAATTGCCTCCTAATCATAAGTTTTTAAGGGATTATCTTGCAGCTTTAGATCAATCAGATATCACACTCTTTTCA
GCTCAACAGACAGAGTTCTAG

Upstream 100 bases:

>100_bases
AATTTCCGCCTATTGAAGCCATCAAAGTGGCCATTAAAAAGGAAAACCCACCGATTGCAGGCCACTATAAGGCAGTTGGT
ATTGAATTGGAGAGACAGAG

Downstream 100 bases:

>100_bases
ACTCTGTTTTCGGAGTAATGCCTGCTATAAGCCAATCCATAAAGTTTTGCCAAATTCATCGTTATTTTTTCTAAAATTTG
ATATAATAGTATCGGCTTTA

Product: 2-amino-4-hydroxy-6- hydroxymethyldihydropteridine pyrophosphokinase

Products: NA

Alternate protein names: 6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase; PPPK; 7,8-dihydro-6-hydroxymethylpterin-pyrophosphokinase; HPPK

Number of amino acids: Translated: 166; Mature: 165

Protein sequence:

>166_residues
MTIVYLSLGTNMGDRAAYLQKALEALADLPQTRLLAQSSIYETTAWGKTGQADFLNMACQLDTQLTAADFLKETQAIEQS
LGRVRHEKWGSRTIDIDILLFGEEVYDTKELKVPHPYMTERAFVLIPLLELQPDLKLPPNHKFLRDYLAALDQSDITLFS
AQQTEF

Sequences:

>Translated_166_residues
MTIVYLSLGTNMGDRAAYLQKALEALADLPQTRLLAQSSIYETTAWGKTGQADFLNMACQLDTQLTAADFLKETQAIEQS
LGRVRHEKWGSRTIDIDILLFGEEVYDTKELKVPHPYMTERAFVLIPLLELQPDLKLPPNHKFLRDYLAALDQSDITLFS
AQQTEF
>Mature_165_residues
TIVYLSLGTNMGDRAAYLQKALEALADLPQTRLLAQSSIYETTAWGKTGQADFLNMACQLDTQLTAADFLKETQAIEQSL
GRVRHEKWGSRTIDIDILLFGEEVYDTKELKVPHPYMTERAFVLIPLLELQPDLKLPPNHKFLRDYLAALDQSDITLFSA
QQTEF

Specific function: Dihydrofolate biosynthesis; first step. [C]

COG id: COG0801

COG function: function code H; 7,8-dihydro-6-hydroxymethylpterin-pyrophosphokinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HPPK family

Homologues:

Organism=Escherichia coli, GI1786335, Length=139, Percent_Identity=44.6043165467626, Blast_Score=127, Evalue=3e-31,
Organism=Saccharomyces cerevisiae, GI6324073, Length=141, Percent_Identity=39.0070921985816, Blast_Score=94, Evalue=1e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HPPK_STRP1 (P0C0G3)

Other databases:

- EMBL:   AE004092
- EMBL:   CP000017
- RefSeq:   NP_269257.1
- RefSeq:   YP_282187.1
- ProteinModelPortal:   P0C0G3
- SMR:   P0C0G3
- EnsemblBacteria:   EBSTRT00000000347
- EnsemblBacteria:   EBSTRT00000028018
- GeneID:   3572072
- GeneID:   901221
- GenomeReviews:   AE004092_GR
- GenomeReviews:   CP000017_GR
- KEGG:   spy:SPy_1100
- KEGG:   spz:M5005_Spy_0824
- GeneTree:   EBGT00050000027514
- HOGENOM:   HBG662565
- OMA:   ETDPVGY
- ProtClustDB:   CLSK797492
- BioCyc:   SPYO160490:SPY1100-MONOMER
- BioCyc:   SPYO293653:M5005_SPY0824-MONOMER
- InterPro:   IPR000550
- Gene3D:   G3DSA:3.30.70.560
- TIGRFAMs:   TIGR01498

Pfam domain/function: PF01288 HPPK; SSF55083 Hppk

EC number: =2.7.6.3

Molecular weight: Translated: 18824; Mature: 18693

Theoretical pI: Translated: 4.57; Mature: 4.57

Prosite motif: PS00794 HPPK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIVYLSLGTNMGDRAAYLQKALEALADLPQTRLLAQSSIYETTAWGKTGQADFLNMACQ
CEEEEEECCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHH
LDTQLTAADFLKETQAIEQSLGRVRHEKWGSRTIDIDILLFGEEVYDTKELKVPHPYMTE
HCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEEECCHHHCCCCCCCCCCCHHC
RAFVLIPLLELQPDLKLPPNHKFLRDYLAALDQSDITLFSAQQTEF
CCEEEEEHHHCCCCCCCCCCHHHHHHHHHHCCCCCEEEEECCCCCC
>Mature Secondary Structure 
TIVYLSLGTNMGDRAAYLQKALEALADLPQTRLLAQSSIYETTAWGKTGQADFLNMACQ
EEEEEECCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHH
LDTQLTAADFLKETQAIEQSLGRVRHEKWGSRTIDIDILLFGEEVYDTKELKVPHPYMTE
HCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEEECCHHHCCCCCCCCCCCHHC
RAFVLIPLLELQPDLKLPPNHKFLRDYLAALDQSDITLFSAQQTEF
CCEEEEEHHHCCCCCCCCCCHHHHHHHHHHCCCCCEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11296296