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The map label for this gene is murB

Identifier: 15675084

GI number: 15675084

Start: 900426

End: 901313

Strand: Direct

Name: murB

Synonym: SPy_1101

Alternate gene names: 15675084

Gene position: 900426-901313 (Clockwise)

Preceding gene: 15675083

Following gene: 15675085

Centisome position: 48.61

GC content: 41.33

Gene sequence:

>888_bases
ATGATAACTGAACTTCATGGGATTGATATTCGAGAGAATGAACCCCTAAAACATTACACTTACACTAAGGTAGGTGGCCC
AGCAGATTTCTTGGCTTTTCCTAGAAATCACTACGAGCTATCACGCATCGTTGCTTATGCCAACAAAGAAAATATGCCTT
GGCTTGTTTTGGGAAATGCCAGCAATCTCATTGTGCGAGATGGTGGTATTCGTGGCTTTGTCATCATGTTTGATAAGCTA
AATGCAGTGCACTTAAATGGTTATACCTTAGAAGCCGAAGCTGGTGCCAATCTGATTGAAACAACGAAGATTGCCAAATT
CCATAGTTTAACAGGGTTTGAATTTGCATGTGGCATTCCTGGAAGCATTGGGGGTGCTGTTTTTATGAATGCGGGTGCTT
ATGGTGGGGAAATATCACATATTTTCTTATCCGCTAAGGTGCTTACCCCAAGTGGAGAGATCAAAACCATTTCAGCTAGG
GATATGGCTTTTGGTTACCGTCACTCTGCCATTCAAGAAACAGGTGACATTGTCATTTCTGCTAAGTTTGCTCTTAAACC
GGGTAATTATGATACGATCAGCCAAGAAATGAATCGGTTAAATCACCTTCGCCAACTAAAACAACCTTTAGAATTTCCAT
CTTGTGGATCTGTGTTTAAGCGCCCGCCAGGACACTTTGCAGGTCAATTAATCATGGAAGCAAATCTTAAAGGGCATCGG
ATTGGTGGTGTGGAAGTTTCCGAAAAACACACTGGTTTTATGATTAATGTGGCAGATGGCACAGCTAAAGATTATGAAGA
TTTGATTGCTTATGTCATTGAGACAGTTGAAAACCATTCTGGTGTCAGGCTTGAACCAGAAGTTCGGATTATTGGGGAAA
ACCTGTAA

Upstream 100 bases:

>100_bases
CCAAATTCATCGTTATTTTTTCTAAAATTTGATATAATAGTATCGGCTTTATGCCGATTTTTTTACGTGTATAAGAAGTG
ATAAAAGAAAGAAGATAACT

Downstream 100 bases:

>100_bases
CCATTATTGATAATTAAAGAAAGTACCGTGGAGGATTTATGACAATTGACTAAGCCAATTATCACATTTAACAATGTTTC
AAAAACATTTGAGGACAGTG

Product: UDP-N-acetylenolpyruvoylglucosamine reductase

Products: NA

Alternate protein names: UDP-N-acetylmuramate dehydrogenase

Number of amino acids: Translated: 295; Mature: 295

Protein sequence:

>295_residues
MITELHGIDIRENEPLKHYTYTKVGGPADFLAFPRNHYELSRIVAYANKENMPWLVLGNASNLIVRDGGIRGFVIMFDKL
NAVHLNGYTLEAEAGANLIETTKIAKFHSLTGFEFACGIPGSIGGAVFMNAGAYGGEISHIFLSAKVLTPSGEIKTISAR
DMAFGYRHSAIQETGDIVISAKFALKPGNYDTISQEMNRLNHLRQLKQPLEFPSCGSVFKRPPGHFAGQLIMEANLKGHR
IGGVEVSEKHTGFMINVADGTAKDYEDLIAYVIETVENHSGVRLEPEVRIIGENL

Sequences:

>Translated_295_residues
MITELHGIDIRENEPLKHYTYTKVGGPADFLAFPRNHYELSRIVAYANKENMPWLVLGNASNLIVRDGGIRGFVIMFDKL
NAVHLNGYTLEAEAGANLIETTKIAKFHSLTGFEFACGIPGSIGGAVFMNAGAYGGEISHIFLSAKVLTPSGEIKTISAR
DMAFGYRHSAIQETGDIVISAKFALKPGNYDTISQEMNRLNHLRQLKQPLEFPSCGSVFKRPPGHFAGQLIMEANLKGHR
IGGVEVSEKHTGFMINVADGTAKDYEDLIAYVIETVENHSGVRLEPEVRIIGENL
>Mature_295_residues
MITELHGIDIRENEPLKHYTYTKVGGPADFLAFPRNHYELSRIVAYANKENMPWLVLGNASNLIVRDGGIRGFVIMFDKL
NAVHLNGYTLEAEAGANLIETTKIAKFHSLTGFEFACGIPGSIGGAVFMNAGAYGGEISHIFLSAKVLTPSGEIKTISAR
DMAFGYRHSAIQETGDIVISAKFALKPGNYDTISQEMNRLNHLRQLKQPLEFPSCGSVFKRPPGHFAGQLIMEANLKGHR
IGGVEVSEKHTGFMINVADGTAKDYEDLIAYVIETVENHSGVRLEPEVRIIGENL

Specific function: Cell wall formation

COG id: COG0812

COG function: function code M; UDP-N-acetylmuramate dehydrogenase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding PCMH-type domain

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MURB_STRP1 (Q99ZS9)

Other databases:

- EMBL:   AE004092
- EMBL:   CP000017
- RefSeq:   NP_269258.1
- RefSeq:   YP_282188.1
- ProteinModelPortal:   Q99ZS9
- SMR:   Q99ZS9
- EnsemblBacteria:   EBSTRT00000001260
- EnsemblBacteria:   EBSTRT00000027726
- GeneID:   3572073
- GeneID:   901222
- GenomeReviews:   AE004092_GR
- GenomeReviews:   CP000017_GR
- KEGG:   spy:SPy_1101
- KEGG:   spz:M5005_Spy_0825
- GeneTree:   EBGT00050000027116
- HOGENOM:   HBG686573
- OMA:   SKKHAGF
- ProtClustDB:   PRK13905
- BioCyc:   SPYO160490:SPY1101-MONOMER
- BioCyc:   SPYO293653:M5005_SPY0825-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00037
- InterPro:   IPR016169
- InterPro:   IPR016166
- InterPro:   IPR016167
- InterPro:   IPR003170
- InterPro:   IPR011601
- InterPro:   IPR006094
- Gene3D:   G3DSA:3.30.465.10
- Gene3D:   G3DSA:3.30.43.10
- Gene3D:   G3DSA:3.90.78.10
- PANTHER:   PTHR21071
- TIGRFAMs:   TIGR00179

Pfam domain/function: PF01565 FAD_binding_4; PF02873 MurB_C; SSF56176 FAD-binding_2; SSF56194 MurB_C

EC number: =1.1.1.158

Molecular weight: Translated: 32375; Mature: 32375

Theoretical pI: Translated: 6.65; Mature: 6.65

Prosite motif: PS51387 FAD_PCMH

Important sites: ACT_SITE 167-167 ACT_SITE 217-217 ACT_SITE 287-287

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MITELHGIDIRENEPLKHYTYTKVGGPADFLAFPRNHYELSRIVAYANKENMPWLVLGNA
CCCCCCCCCCCCCCCCCEEEEEECCCCHHHHCCCCCHHHHHHHHEECCCCCCCEEEECCC
SNLIVRDGGIRGFVIMFDKLNAVHLNGYTLEAEAGANLIETTKIAKFHSLTGFEFACGIP
CEEEEECCCCEEEEEEEECCCEEEECCEEEECCCCCCCHHHHHHHHHHCCCCCEEECCCC
GSIGGAVFMNAGAYGGEISHIFLSAKVLTPSGEIKTISARDMAFGYRHSAIQETGDIVIS
CCCCCEEEEECCCCCCCEEEEEEEEEEECCCCCEEEEEHHHHHCCHHHHHHHCCCCEEEE
AKFALKPGNYDTISQEMNRLNHLRQLKQPLEFPSCGSVFKRPPGHFAGQLIMEANLKGHR
EEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHCCCCCHHHEEEEEEECCCCEE
IGGVEVSEKHTGFMINVADGTAKDYEDLIAYVIETVENHSGVRLEPEVRIIGENL
ECCEEECCCCCCEEEEECCCCCCCHHHHHHHHHHHHHCCCCEEECCCEEEEECCC
>Mature Secondary Structure
MITELHGIDIRENEPLKHYTYTKVGGPADFLAFPRNHYELSRIVAYANKENMPWLVLGNA
CCCCCCCCCCCCCCCCCEEEEEECCCCHHHHCCCCCHHHHHHHHEECCCCCCCEEEECCC
SNLIVRDGGIRGFVIMFDKLNAVHLNGYTLEAEAGANLIETTKIAKFHSLTGFEFACGIP
CEEEEECCCCEEEEEEEECCCEEEECCEEEECCCCCCCHHHHHHHHHHCCCCCEEECCCC
GSIGGAVFMNAGAYGGEISHIFLSAKVLTPSGEIKTISARDMAFGYRHSAIQETGDIVIS
CCCCCEEEEECCCCCCCEEEEEEEEEEECCCCCEEEEEHHHHHCCHHHHHHHCCCCEEEE
AKFALKPGNYDTISQEMNRLNHLRQLKQPLEFPSCGSVFKRPPGHFAGQLIMEANLKGHR
EEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHCCCCCHHHEEEEEEECCCCEE
IGGVEVSEKHTGFMINVADGTAKDYEDLIAYVIETVENHSGVRLEPEVRIIGENL
ECCEEECCCCCCEEEEECCCCCCCHHHHHHHHHHHHHCCCCEEECCCEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11296296