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The map label for this gene is pyk

Identifier: 15673314

GI number: 15673314

Start: 1370319

End: 1371827

Strand: Reverse

Name: pyk

Synonym: L0003

Alternate gene names: 15673314

Gene position: 1371827-1370319 (Counterclockwise)

Preceding gene: 15673315

Following gene: 15673313

Centisome position: 57.99

GC content: 40.29

Gene sequence:

>1509_bases
ATGAATAAACGTGTAAAAATCGTCTCAACTCTTGGACCTGCTGTCGAAATTCGTGGTGGCAAAAAATTTGGTGAAAGCGG
CTATTGGGGAGAATCTCTTGACGTCGAAGCATCAGCAAAAAACATTGCAGCTTTGATTGAAGAAGGCGCAAATGTCTTCC
GTTTCAACTTCTCACACGGTGACCATCCTGAACAAGGTGCTCGTATGGCAACAGTTCACCGCGCTGAAGAAATCGCTGGT
CATAAAGTAGGTTTCTTGCTTGATACTAAAGGTCCTGAAATGCGTACAGAACTCTTTGCTGACGGTGCTGATGCAATCAG
CGTTGTTACTGGAGATAAATTCCGCGTTGCAACTAAACAAGGTCTTAAATCAACTCCTGAATTGATTGCTTTGAACGTAG
CTGGTGGTCTTGATATCTTTGATGATGTTGAAATCGGTCAAACTATTTTGATTGACGATGGTAAACTTGGTTTGTCATTG
ACTGGTAAAGATGCAGCAACTCGTGAGTTTGAAGTTGAAGCACAAAATGATGGCGTTATTGGTAAACAAAAAGGTGTTAA
CATTCCTAACACTAAAATTCCTTTCCCAGCTCTTGCTGAACGTGATGATGCGGATATCCGTTTCGGACTTTCACAACCTG
GTGGAATCAACTTTATCGCTATCTCATTCGTACGTACTGCAAATGACGTTAAAGAAGTACGTCGTATTTGTGAAGAAACT
GGAAATCCACACGTACAACTTTTGGCTAAAATTGAAAACCAACAAGGTATCGAAAACCTTGATGAAATCATCGAAGCAGC
TGATGGTATCATGATTGCTCGTGGTGACATGGGTATCGAAGTTCCATTTGAAATGGTTCCAGTTTACCAAAAATTGATCA
TCAGCAAAGTTAACAAAGCAGGTAAAATCGTTGTTACTGCAACAAACATGCTTGAATCAATGACTTACAACCCACGTGCT
ACTCGTTCAGAAATTTCAGACGTATTCAACGCTGTTATTGACGGAACTGACGCAACAATGCTTTCAGGTGAATCTGCTAA
TGGTAAATACCCACGCGAATCAGTTCGTACAATGGCTACTGTTAACAAAAATGCTCAAACAATGTTGAAAGAATACGGAC
GTCTTCACCCAGAACGTTACGACAAATCAACTGTAACAGAAGTAGTTGCTGCATCAGTTAAAAACGCTGCAGAAGCTATG
GATATCAAATTGATTGTTGCTTTGACTGAATCAGGTAACACTGCTCGTTTGATTTCTAAACACCGTCCTAATGCTGATAT
CTTGGCTATTACATTTGACGAAAAAGTTGAACGTGGATTGATGATTAACTGGGGTGTAATCCCAACAATGACTGAAAAAC
CTTCATCTACAGATGACATGTTTGAAGTTGCTGAAAAAGTTGCTTTGGCTTCAGGTTTAGTTGAATCTGGTGACAATATC
ATCATCGTTGCTGGTGTTCCAGTTGGTACTGGTCGTACAAATACAATGCGTATCCGCACTGTTAAATAA

Upstream 100 bases:

>100_bases
TAACCTTAACTAAGTTACTGCAAATCTGTTTCTAGTTAAGTGTTAAACGCATAATTAGGGCAGAGATATATAATTAATCA
TTATAGGAGAAAAACACAAA

Downstream 100 bases:

>100_bases
TTTGTCAATTAAGAATCTTAAAACAATAATGCCTTCCGCTATCGACAGGCCCTATTGTTGAAAAGATTATATTTTATAAA
ATACTCGTTTATAATTTGAA

Product: pyruvate kinase

Products: NA

Alternate protein names: PK

Number of amino acids: Translated: 502; Mature: 502

Protein sequence:

>502_residues
MNKRVKIVSTLGPAVEIRGGKKFGESGYWGESLDVEASAKNIAALIEEGANVFRFNFSHGDHPEQGARMATVHRAEEIAG
HKVGFLLDTKGPEMRTELFADGADAISVVTGDKFRVATKQGLKSTPELIALNVAGGLDIFDDVEIGQTILIDDGKLGLSL
TGKDAATREFEVEAQNDGVIGKQKGVNIPNTKIPFPALAERDDADIRFGLSQPGGINFIAISFVRTANDVKEVRRICEET
GNPHVQLLAKIENQQGIENLDEIIEAADGIMIARGDMGIEVPFEMVPVYQKLIISKVNKAGKIVVTATNMLESMTYNPRA
TRSEISDVFNAVIDGTDATMLSGESANGKYPRESVRTMATVNKNAQTMLKEYGRLHPERYDKSTVTEVVAASVKNAAEAM
DIKLIVALTESGNTARLISKHRPNADILAITFDEKVERGLMINWGVIPTMTEKPSSTDDMFEVAEKVALASGLVESGDNI
IIVAGVPVGTGRTNTMRIRTVK

Sequences:

>Translated_502_residues
MNKRVKIVSTLGPAVEIRGGKKFGESGYWGESLDVEASAKNIAALIEEGANVFRFNFSHGDHPEQGARMATVHRAEEIAG
HKVGFLLDTKGPEMRTELFADGADAISVVTGDKFRVATKQGLKSTPELIALNVAGGLDIFDDVEIGQTILIDDGKLGLSL
TGKDAATREFEVEAQNDGVIGKQKGVNIPNTKIPFPALAERDDADIRFGLSQPGGINFIAISFVRTANDVKEVRRICEET
GNPHVQLLAKIENQQGIENLDEIIEAADGIMIARGDMGIEVPFEMVPVYQKLIISKVNKAGKIVVTATNMLESMTYNPRA
TRSEISDVFNAVIDGTDATMLSGESANGKYPRESVRTMATVNKNAQTMLKEYGRLHPERYDKSTVTEVVAASVKNAAEAM
DIKLIVALTESGNTARLISKHRPNADILAITFDEKVERGLMINWGVIPTMTEKPSSTDDMFEVAEKVALASGLVESGDNI
IIVAGVPVGTGRTNTMRIRTVK
>Mature_502_residues
MNKRVKIVSTLGPAVEIRGGKKFGESGYWGESLDVEASAKNIAALIEEGANVFRFNFSHGDHPEQGARMATVHRAEEIAG
HKVGFLLDTKGPEMRTELFADGADAISVVTGDKFRVATKQGLKSTPELIALNVAGGLDIFDDVEIGQTILIDDGKLGLSL
TGKDAATREFEVEAQNDGVIGKQKGVNIPNTKIPFPALAERDDADIRFGLSQPGGINFIAISFVRTANDVKEVRRICEET
GNPHVQLLAKIENQQGIENLDEIIEAADGIMIARGDMGIEVPFEMVPVYQKLIISKVNKAGKIVVTATNMLESMTYNPRA
TRSEISDVFNAVIDGTDATMLSGESANGKYPRESVRTMATVNKNAQTMLKEYGRLHPERYDKSTVTEVVAASVKNAAEAM
DIKLIVALTESGNTARLISKHRPNADILAITFDEKVERGLMINWGVIPTMTEKPSSTDDMFEVAEKVALASGLVESGDNI
IIVAGVPVGTGRTNTMRIRTVK

Specific function: Glycolysis; final step. [C]

COG id: COG0469

COG function: function code G; Pyruvate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pyruvate kinase family

Homologues:

Organism=Homo sapiens, GI33286418, Length=485, Percent_Identity=37.5257731958763, Blast_Score=299, Evalue=5e-81,
Organism=Homo sapiens, GI33286422, Length=481, Percent_Identity=37.2141372141372, Blast_Score=293, Evalue=2e-79,
Organism=Homo sapiens, GI33286420, Length=481, Percent_Identity=37.2141372141372, Blast_Score=293, Evalue=2e-79,
Organism=Homo sapiens, GI10835121, Length=485, Percent_Identity=36.0824742268041, Blast_Score=286, Evalue=4e-77,
Organism=Homo sapiens, GI32967597, Length=485, Percent_Identity=36.0824742268041, Blast_Score=285, Evalue=5e-77,
Organism=Homo sapiens, GI310128732, Length=282, Percent_Identity=37.5886524822695, Blast_Score=175, Evalue=7e-44,
Organism=Homo sapiens, GI310128730, Length=282, Percent_Identity=37.5886524822695, Blast_Score=175, Evalue=7e-44,
Organism=Homo sapiens, GI310128736, Length=230, Percent_Identity=33.4782608695652, Blast_Score=119, Evalue=7e-27,
Organism=Homo sapiens, GI310128734, Length=230, Percent_Identity=33.4782608695652, Blast_Score=119, Evalue=7e-27,
Organism=Homo sapiens, GI310128738, Length=223, Percent_Identity=34.0807174887892, Blast_Score=118, Evalue=1e-26,
Organism=Escherichia coli, GI1787965, Length=499, Percent_Identity=39.2785571142285, Blast_Score=366, Evalue=1e-102,
Organism=Escherichia coli, GI1788160, Length=508, Percent_Identity=32.2834645669291, Blast_Score=214, Evalue=1e-56,
Organism=Caenorhabditis elegans, GI17544584, Length=518, Percent_Identity=36.1003861003861, Blast_Score=305, Evalue=3e-83,
Organism=Caenorhabditis elegans, GI17506829, Length=512, Percent_Identity=35.546875, Blast_Score=277, Evalue=9e-75,
Organism=Caenorhabditis elegans, GI71984413, Length=512, Percent_Identity=35.546875, Blast_Score=277, Evalue=1e-74,
Organism=Caenorhabditis elegans, GI71984406, Length=512, Percent_Identity=35.546875, Blast_Score=277, Evalue=1e-74,
Organism=Caenorhabditis elegans, GI17506831, Length=510, Percent_Identity=35.6862745098039, Blast_Score=277, Evalue=1e-74,
Organism=Saccharomyces cerevisiae, GI6319279, Length=477, Percent_Identity=37.5262054507338, Blast_Score=289, Evalue=8e-79,
Organism=Saccharomyces cerevisiae, GI6324923, Length=511, Percent_Identity=36.2035225048924, Blast_Score=274, Evalue=2e-74,
Organism=Drosophila melanogaster, GI24648964, Length=515, Percent_Identity=37.4757281553398, Blast_Score=307, Evalue=9e-84,
Organism=Drosophila melanogaster, GI28571814, Length=515, Percent_Identity=37.4757281553398, Blast_Score=306, Evalue=1e-83,
Organism=Drosophila melanogaster, GI24648966, Length=416, Percent_Identity=37.7403846153846, Blast_Score=254, Evalue=9e-68,
Organism=Drosophila melanogaster, GI24581235, Length=481, Percent_Identity=32.8482328482328, Blast_Score=240, Evalue=2e-63,
Organism=Drosophila melanogaster, GI24646914, Length=264, Percent_Identity=35.9848484848485, Blast_Score=163, Evalue=3e-40,

Paralogues:

None

Copy number: 500 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 124 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): KPYK_LACLA (Q07637)

Other databases:

- EMBL:   L07920
- EMBL:   AE005176
- PIR:   B40620
- PIR:   D86791
- RefSeq:   NP_267488.1
- ProteinModelPortal:   Q07637
- GeneID:   1114982
- GenomeReviews:   AE005176_GR
- KEGG:   lla:L0003
- NMPDR:   fig|272623.1.peg.1367
- HOGENOM:   HBG734376
- OMA:   HADHKTH
- ProtClustDB:   PRK05826
- BioCyc:   LLAC272623:L0003-MONOMER
- BioCyc:   MetaCyc:MONOMER-13043
- BRENDA:   2.7.1.40
- GO:   GO:0006096
- InterPro:   IPR001697
- InterPro:   IPR015813
- InterPro:   IPR011037
- InterPro:   IPR015794
- InterPro:   IPR018209
- InterPro:   IPR015793
- InterPro:   IPR015795
- InterPro:   IPR015806
- Gene3D:   G3DSA:2.40.33.10
- Gene3D:   G3DSA:3.20.20.60
- Gene3D:   G3DSA:3.40.1380.20
- PANTHER:   PTHR11817
- PRINTS:   PR01050
- TIGRFAMs:   TIGR01064

Pfam domain/function: PF00224 PK; PF02887 PK_C; SSF50800 PK_B_barrel_like; SSF52935 Pyruvate_kinase; SSF51621 Pyrv/PenolPyrv_Kinase_cat

EC number: =2.7.1.40

Molecular weight: Translated: 54256; Mature: 54256

Theoretical pI: Translated: 5.04; Mature: 5.04

Prosite motif: PS00110 PYRUVATE_KINASE

Important sites: BINDING 54-54 BINDING 275-275 BINDING 276-276 BINDING 308-308

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKRVKIVSTLGPAVEIRGGKKFGESGYWGESLDVEASAKNIAALIEEGANVFRFNFSHG
CCCEEEEEEECCCEEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCEEEEECCCC
DHPEQGARMATVHRAEEIAGHKVGFLLDTKGPEMRTELFADGADAISVVTGDKFRVATKQ
CCCCCCCEEHHHHHHHHHCCCEEEEEEECCCCHHHHHHHCCCCCEEEEEECCEEEEEHHH
GLKSTPELIALNVAGGLDIFDDVEIGQTILIDDGKLGLSLTGKDAATREFEVEAQNDGVI
CCCCCCCEEEEEECCCCCCCCCCCCCCEEEEECCCEEEEECCCCCCCEEEEEEECCCCCE
GKQKGVNIPNTKIPFPALAERDDADIRFGLSQPGGINFIAISFVRTANDVKEVRRICEET
ECCCCCCCCCCCCCCCCCCCCCCCCEEECCCCCCCCEEEEEEEEHHHHHHHHHHHHHHHC
GNPHVQLLAKIENQQGIENLDEIIEAADGIMIARGDMGIEVPFEMVPVYQKLIISKVNKA
CCCEEEEEEEHHCCCCHHHHHHHHHHHCCEEEEECCCCCCCCHHHHHHHHHHHHHHCCCC
GKIVVTATNMLESMTYNPRATRSEISDVFNAVIDGTDATMLSGESANGKYPRESVRTMAT
CEEEEEEHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCHHHHHHHHH
VNKNAQTMLKEYGRLHPERYDKSTVTEVVAASVKNAAEAMDIKLIVALTESGNTARLISK
CCCCHHHHHHHHHCCCCHHCCHHHHHHHHHHHHHHHHHHCEEEEEEEEECCCCEEEHHHC
HRPNADILAITFDEKVERGLMINWGVIPTMTEKPSSTDDMFEVAEKVALASGLVESGDNI
CCCCCCEEEEEECCHHCCCEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCE
IIVAGVPVGTGRTNTMRIRTVK
EEEEECCCCCCCCCEEEEEEEC
>Mature Secondary Structure
MNKRVKIVSTLGPAVEIRGGKKFGESGYWGESLDVEASAKNIAALIEEGANVFRFNFSHG
CCCEEEEEEECCCEEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCEEEEECCCC
DHPEQGARMATVHRAEEIAGHKVGFLLDTKGPEMRTELFADGADAISVVTGDKFRVATKQ
CCCCCCCEEHHHHHHHHHCCCEEEEEEECCCCHHHHHHHCCCCCEEEEEECCEEEEEHHH
GLKSTPELIALNVAGGLDIFDDVEIGQTILIDDGKLGLSLTGKDAATREFEVEAQNDGVI
CCCCCCCEEEEEECCCCCCCCCCCCCCEEEEECCCEEEEECCCCCCCEEEEEEECCCCCE
GKQKGVNIPNTKIPFPALAERDDADIRFGLSQPGGINFIAISFVRTANDVKEVRRICEET
ECCCCCCCCCCCCCCCCCCCCCCCCEEECCCCCCCCEEEEEEEEHHHHHHHHHHHHHHHC
GNPHVQLLAKIENQQGIENLDEIIEAADGIMIARGDMGIEVPFEMVPVYQKLIISKVNKA
CCCEEEEEEEHHCCCCHHHHHHHHHHHCCEEEEECCCCCCCCHHHHHHHHHHHHHHCCCC
GKIVVTATNMLESMTYNPRATRSEISDVFNAVIDGTDATMLSGESANGKYPRESVRTMAT
CEEEEEEHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCHHHHHHHHH
VNKNAQTMLKEYGRLHPERYDKSTVTEVVAASVKNAAEAMDIKLIVALTESGNTARLISK
CCCCHHHHHHHHHCCCCHHCCHHHHHHHHHHHHHHHHHHCEEEEEEEEECCCCEEEHHHC
HRPNADILAITFDEKVERGLMINWGVIPTMTEKPSSTDDMFEVAEKVALASGLVESGDNI
CCCCCCEEEEEECCHHCCCEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCE
IIVAGVPVGTGRTNTMRIRTVK
EEEEECCCCCCCCCEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8478320; 11337471