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The map label for this gene is ldh

Identifier: 15673313

GI number: 15673313

Start: 1369224

End: 1370201

Strand: Reverse

Name: ldh

Synonym: L0017

Alternate gene names: 15673313

Gene position: 1370201-1369224 (Counterclockwise)

Preceding gene: 15673314

Following gene: 15673308

Centisome position: 57.92

GC content: 40.8

Gene sequence:

>978_bases
ATGGCTGATAAACAACGTAAAAAAGTTATCCTTGTAGGTGACGGTGCTGTAGGTTCATCATACGCTTTTGCTCTTGTAAA
CCAAGGGATTGCACAAGAATTAGGAATTGTTGACCTTTTTAAAGAAAAAACTCAAGGAGATGCAGAAGACCTTTCTCATG
CCTTGGCATTTACTTCACCTAAAAAGATTTACTCTGCAGACTACTCTGATGCAAGCGACGCTGACCTCGTAGTCTTGACT
TCTGGTGCTCCACAAAAACCAGGTGAAACTCGTCTTGACCTTGTTGAAAAAAATCTTCGTATCACTAAAGATGTTGTCAC
TAAAATTGTTGCTTCAGGTTTCAAAGGAATCTTCCTTGTTGCTGCTAACCCAGTTGATATCTTGACATACGCTACTTGGA
AATTCTCAGGTTTCCCTAAAAACCGCGTTGTAGGTTCAGGTACTTCACTTGATACTGCACGTTTCCGTCAAGCATTGGCA
GAAAAAGTTGATGTTGACGCTCGTTCAATCCACGCATACATCATGGGTGAACACGGTGACTCAGAATTTGCCGTTTGGTC
ACACGCTAACGTTGCTGGTGTTAAATTGGAACAATGGTTCCAAGAAAATGACTACCTTAACGAAGCTGAAATCGTTGAAT
TGTTTGAATCTGTACGTGATGCTGCTTACTCAATCATCGCTAAAAAAGGTGCAACATTCTATGGTGTCGCTGTAGCTCTT
GCTCGTATTACTAAAGCAATTCTTGATGATGAACATGCAGTACTTCCAGTATCAGTATTCCAAGATGGACAATATGGCGT
AAGCGACTGCTACCTTGGTCAACCAGCTGTAGTTGGTGCTGAAGGTGTTGTTAACCCAATCCACATTCCATTGAATGATG
CTGAAATGCAAAAAATGGAAGCTTCTGGTGCTCAATTGAAAGCAATCATTGACGAAGCTTTTGCTAAAGAAGAATTTGCT
TCTGCAGTTAAAAACTAA

Upstream 100 bases:

>100_bases
TTAAAACAATAATGCCTTCCGCTATCGACAGGCCCTATTGTTGAAAAGATTATATTTTATAAAATACTCGTTTATAATTT
GAAAGGAATTAAAGAAAATC

Downstream 100 bases:

>100_bases
AAATAAAAAGAGTTGGTTGAGATTTCAACTAGCTCTTTTTATTTTAAATTAAATTTGTTAAGCTAAAAGTATAATAAAAA
AGTCTGTTCATCAACAGACT

Product: L-lactate dehydrogenase

Products: NA

Alternate protein names: L-LDH 1

Number of amino acids: Translated: 325; Mature: 324

Protein sequence:

>325_residues
MADKQRKKVILVGDGAVGSSYAFALVNQGIAQELGIVDLFKEKTQGDAEDLSHALAFTSPKKIYSADYSDASDADLVVLT
SGAPQKPGETRLDLVEKNLRITKDVVTKIVASGFKGIFLVAANPVDILTYATWKFSGFPKNRVVGSGTSLDTARFRQALA
EKVDVDARSIHAYIMGEHGDSEFAVWSHANVAGVKLEQWFQENDYLNEAEIVELFESVRDAAYSIIAKKGATFYGVAVAL
ARITKAILDDEHAVLPVSVFQDGQYGVSDCYLGQPAVVGAEGVVNPIHIPLNDAEMQKMEASGAQLKAIIDEAFAKEEFA
SAVKN

Sequences:

>Translated_325_residues
MADKQRKKVILVGDGAVGSSYAFALVNQGIAQELGIVDLFKEKTQGDAEDLSHALAFTSPKKIYSADYSDASDADLVVLT
SGAPQKPGETRLDLVEKNLRITKDVVTKIVASGFKGIFLVAANPVDILTYATWKFSGFPKNRVVGSGTSLDTARFRQALA
EKVDVDARSIHAYIMGEHGDSEFAVWSHANVAGVKLEQWFQENDYLNEAEIVELFESVRDAAYSIIAKKGATFYGVAVAL
ARITKAILDDEHAVLPVSVFQDGQYGVSDCYLGQPAVVGAEGVVNPIHIPLNDAEMQKMEASGAQLKAIIDEAFAKEEFA
SAVKN
>Mature_324_residues
ADKQRKKVILVGDGAVGSSYAFALVNQGIAQELGIVDLFKEKTQGDAEDLSHALAFTSPKKIYSADYSDASDADLVVLTS
GAPQKPGETRLDLVEKNLRITKDVVTKIVASGFKGIFLVAANPVDILTYATWKFSGFPKNRVVGSGTSLDTARFRQALAE
KVDVDARSIHAYIMGEHGDSEFAVWSHANVAGVKLEQWFQENDYLNEAEIVELFESVRDAAYSIIAKKGATFYGVAVALA
RITKAILDDEHAVLPVSVFQDGQYGVSDCYLGQPAVVGAEGVVNPIHIPLNDAEMQKMEASGAQLKAIIDEAFAKEEFAS
AVKN

Specific function: Catalyzes The Reversible Oxidation Of Malate To Oxaloacetate. [C]

COG id: COG0039

COG function: function code C; Malate/lactate dehydrogenases

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the LDH/MDH superfamily. LDH family

Homologues:

Organism=Homo sapiens, GI291575128, Length=302, Percent_Identity=38.7417218543046, Blast_Score=231, Evalue=5e-61,
Organism=Homo sapiens, GI4557032, Length=302, Percent_Identity=38.7417218543046, Blast_Score=231, Evalue=5e-61,
Organism=Homo sapiens, GI15082234, Length=308, Percent_Identity=37.987012987013, Blast_Score=226, Evalue=3e-59,
Organism=Homo sapiens, GI5031857, Length=310, Percent_Identity=37.0967741935484, Blast_Score=224, Evalue=8e-59,
Organism=Homo sapiens, GI260099723, Length=310, Percent_Identity=37.0967741935484, Blast_Score=224, Evalue=9e-59,
Organism=Homo sapiens, GI47059044, Length=311, Percent_Identity=37.620578778135, Blast_Score=222, Evalue=3e-58,
Organism=Homo sapiens, GI221136809, Length=311, Percent_Identity=37.620578778135, Blast_Score=222, Evalue=3e-58,
Organism=Homo sapiens, GI9257228, Length=296, Percent_Identity=39.527027027027, Blast_Score=210, Evalue=1e-54,
Organism=Homo sapiens, GI4504973, Length=296, Percent_Identity=39.527027027027, Blast_Score=210, Evalue=1e-54,
Organism=Homo sapiens, GI260099727, Length=193, Percent_Identity=44.559585492228, Blast_Score=186, Evalue=3e-47,
Organism=Homo sapiens, GI260099725, Length=193, Percent_Identity=44.559585492228, Blast_Score=186, Evalue=3e-47,
Organism=Homo sapiens, GI207028494, Length=195, Percent_Identity=37.4358974358974, Blast_Score=143, Evalue=2e-34,
Organism=Homo sapiens, GI103472011, Length=311, Percent_Identity=26.3665594855305, Blast_Score=119, Evalue=3e-27,
Organism=Homo sapiens, GI103472015, Length=185, Percent_Identity=30.8108108108108, Blast_Score=99, Evalue=6e-21,
Organism=Escherichia coli, GI1789632, Length=291, Percent_Identity=26.4604810996564, Blast_Score=63, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17535107, Length=291, Percent_Identity=40.893470790378, Blast_Score=224, Evalue=5e-59,
Organism=Caenorhabditis elegans, GI17554310, Length=296, Percent_Identity=28.0405405405405, Blast_Score=82, Evalue=3e-16,
Organism=Drosophila melanogaster, GI17136226, Length=301, Percent_Identity=38.2059800664452, Blast_Score=223, Evalue=2e-58,
Organism=Drosophila melanogaster, GI45550422, Length=302, Percent_Identity=30.794701986755, Blast_Score=159, Evalue=3e-39,
Organism=Drosophila melanogaster, GI24647881, Length=331, Percent_Identity=27.4924471299094, Blast_Score=78, Evalue=7e-15,

Paralogues:

None

Copy number: 2640 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2380 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 260 Molecules/Cell In: Stationary Phase

Swissprot (AC and ID): LDH1_LACLA (Q01462)

Other databases:

- EMBL:   M95919
- EMBL:   M88490
- EMBL:   L07920
- EMBL:   U04315
- EMBL:   U02386
- EMBL:   U78632
- EMBL:   U78636
- EMBL:   U78637
- EMBL:   U78638
- EMBL:   U78634
- EMBL:   AE005176
- PIR:   A45246
- PIR:   C86791
- PIR:   JN0449
- RefSeq:   NP_267487.1
- ProteinModelPortal:   Q01462
- SMR:   Q01462
- PhosSite:   Q01462
- GeneID:   1114981
- GenomeReviews:   AE005176_GR
- KEGG:   lla:L0017
- NMPDR:   fig|272623.1.peg.1366
- HOGENOM:   HBG566126
- OMA:   MDLNHGV
- ProtClustDB:   PRK00066
- BioCyc:   LLAC272623:L0017-MONOMER
- BRENDA:   1.1.1.27
- GO:   GO:0005737
- GO:   GO:0005488
- GO:   GO:0006096
- HAMAP:   MF_00488
- InterPro:   IPR001557
- InterPro:   IPR011304
- InterPro:   IPR018177
- InterPro:   IPR022383
- InterPro:   IPR001236
- InterPro:   IPR015955
- InterPro:   IPR016040
- Gene3D:   G3DSA:3.90.110.10
- Gene3D:   G3DSA:3.40.50.720
- PIRSF:   PIRSF000102
- PRINTS:   PR00086
- TIGRFAMs:   TIGR01771

Pfam domain/function: PF02866 Ldh_1_C; PF00056 Ldh_1_N; SSF56327 Lactate_DH/Glyco_hydro_4_C

EC number: =1.1.1.27

Molecular weight: Translated: 35051; Mature: 34920

Theoretical pI: Translated: 4.73; Mature: 4.73

Prosite motif: PS00064 L_LDH

Important sites: ACT_SITE 178-178 BINDING 91-91 BINDING 123-123 BINDING 154-154 BINDING 232-232

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADKQRKKVILVGDGAVGSSYAFALVNQGIAQELGIVDLFKEKTQGDAEDLSHALAFTSP
CCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHCHHHHHHHHCCCCHHHHHHHHHCCCC
KKIYSADYSDASDADLVVLTSGAPQKPGETRLDLVEKNLRITKDVVTKIVASGFKGIFLV
HHHEECCCCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEE
AANPVDILTYATWKFSGFPKNRVVGSGTSLDTARFRQALAEKVDVDARSIHAYIMGEHGD
ECCCEEEEEEEEEEECCCCCCCEEECCCCCCHHHHHHHHHHHHCCCHHEEEEEEEECCCC
SEFAVWSHANVAGVKLEQWFQENDYLNEAEIVELFESVRDAAYSIIAKKGATFYGVAVAL
CCEEEEECCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
ARITKAILDDEHAVLPVSVFQDGQYGVSDCYLGQPAVVGAEGVVNPIHIPLNDAEMQKME
HHHHHHHHCCCCCEEEEEEECCCCCCCCCEECCCCCEECCCCCCCCEEECCCHHHHHHHH
ASGAQLKAIIDEAFAKEEFASAVKN
CCCHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
ADKQRKKVILVGDGAVGSSYAFALVNQGIAQELGIVDLFKEKTQGDAEDLSHALAFTSP
CCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHCHHHHHHHHCCCCHHHHHHHHHCCCC
KKIYSADYSDASDADLVVLTSGAPQKPGETRLDLVEKNLRITKDVVTKIVASGFKGIFLV
HHHEECCCCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEE
AANPVDILTYATWKFSGFPKNRVVGSGTSLDTARFRQALAEKVDVDARSIHAYIMGEHGD
ECCCEEEEEEEEEEECCCCCCCEEECCCCCCHHHHHHHHHHHHCCCHHEEEEEEEECCCC
SEFAVWSHANVAGVKLEQWFQENDYLNEAEIVELFESVRDAAYSIIAKKGATFYGVAVAL
CCEEEEECCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
ARITKAILDDEHAVLPVSVFQDGQYGVSDCYLGQPAVVGAEGVVNPIHIPLNDAEMQKME
HHHHHHHHCCCCCEEEEEEECCCCCCCCCEECCCCCEECCCCCCCCEEECCCHHHHHHHH
ASGAQLKAIIDEAFAKEEFASAVKN
CCCHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1400245; 1452029; 8081494; 9023947; 11337471